scholarly journals Comparison of erythrocyte sedimentation rate between blood anticoagulated with sodium citrate and EDTA among Tuberculosis suspected patients at University of Gondar Hospital, North West Ethiopia

2019 ◽  
Author(s):  
Zegeye Getaneh ◽  
Fekadu Ayelgn ◽  
Geletaw Asemahegn ◽  
Habtamu Geleta ◽  
Aregawi Yalew ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Sodium Citrate ◽  
Northwest Ethiopia ◽  
Cross Sectional ◽  
North West ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
The Mean ◽  
Study Participants

Abstract Objective: The purpose of this study was comparison of erythrocyte sedimentation rate results of sodium citrate and ethylene diamine tetra-acetic acid anticoagulant. A comparative cross-sectional study was conducted at University of Gondar Referral Hospital, Northwest, Ethiopia. A total of 70 TB suspected study participants were recruited. From each study participants, 3ml blood was collected in to EDTA tube and 1.6ml blood was collected in to a test tube containing 0.4ml Sodium Citrate anticoagulant. Results: The mean ± SD values for ESR were 57.9 ± 41.45 in EDTA and 50.99 ± 43.5 in citrate anticoagulated blood. The mean difference of ESR values between EDTA and citrated blood was 6.91 ± 13.66 mm/hr with a significant difference. The mean ± SD of ESR for EDTA and citrate blood was 59.57 ± 42.31 and 54.71 ± 40.44 mm/hr for males and 53.57 ± 44.61 and 46.04 ± 41.82 mm/hr for females, respectively. This study indicated that there was a significant difference between ESR values with EDTA and tri-sodium citrate anticoagulants.

scholarly journals A comparison of erythrocyte sedimentation rate between blood anticoagulated with sodium citrate and EDTA among Tuberculosis suspected patients at University of Gondar Hospital, North West Ethiopia

2019 ◽  
Author(s):  
Zegeye Getaneh ◽  
Fekadu Ayelgn ◽  
Geletaw Asemahegn ◽  
Habtamu Geleta ◽  
Aregawi Yalew ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Sodium Citrate ◽  
Northwest Ethiopia ◽  
Cross Sectional ◽  
North West ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
The Mean ◽  
Study Participants

Abstract Objective: The main aim of this study was comparison of erythrocyte sedimentation rate results of sodium citrate and ethylene diamine tetra-acetic acid anticoagulant. A comparative cross-sectional study was conducted at University of Gondar Referral Hospital, Northwest, Ethiopia. A total of 70 TB suspected study participants were recruited. From each study participants, 3ml blood was collected in to EDTA tube and 1.6ml blood was collected in to a test tube containing 0.4ml Sodium Citrate anticoagulant. Results: The mean ± SD values for ESR were 57.9 ± 41.45 in EDTA and 50.99 ± 43.5 in citrate anticoagulated blood. The mean difference of ESR values between EDTA and citrated blood was 6.91 ± 13.66 mm/hr with a significant difference. The mean ± SD of ESR for EDTA and citrate blood was 59.57 ± 42.31 and 54.71 ± 40.44 mm/hr for males and 53.57 ± 44.61 and 46.04 ± 41.82 mm/hr for females, respectively. This study indicated that there was a significant difference between ESR values with EDTA and tri-sodium citrate anticoagulants.

scholarly journals A comparison of erythrocyte sedimentation rates of bloods anticoagulated with trisodium citrate and EDTA among TB presumptive patients at the University of Gondar comprehensive specialized hospital, northwest Ethiopia

2020 ◽  
Author(s):  
Zegeye Getaneh ◽  
Fekadu Ayelgn ◽  
Geletaw Asemahegn ◽  
Habtamu Geleta ◽  
Aregawi Yalew ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Sodium Citrate ◽  
Trisodium Citrate ◽  
Northwest Ethiopia ◽  
Cross Sectional ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
The Mean ◽  
The University

Abstract Objective: The purpose of this study was comparing the erythrocyte sedimentation rate (ESR) results of trisodium citrate (TSC) and ethylene diamine tetra-acetic acid (EDTA) anticoagulants. A comparative cross-sectional study was conducted at the University of Gondar specialized referral hospital, northwest, Ethiopia. A total of 70 TB presumptive participants were recruited. From each of the 70 participants of the study, 3 and 1.6 ml of blood was collected in EDTA tubes and 0.4 ml of trisodium Citrate anticoagulant containing test tubes, respectively. Results: The mean ± SD values of ESR were 57.9 ± 41.45 mm/hr in EDTA and 50.99 ± 43.5 mm/hr in TSC anticoagulated blood. The mean difference of ESR values between EDTA and TSC blood (6.91 ± 13.66 mm/hr) was statistically significant. The Mean ± SD of ESR values using EDTA and TSC in males were 59.57 ± 42.31 and 53.57 ± 44.61 mm/hr while for females it was 54.71±40.44 and 46.04 ± 41.82 mm/hr, respectively. The study indicated that there was a significant difference between ESR values with EDTA and tri-sodium citrate anticoagulants. Keywords: Erythrocyte sedimentation rate, EDTA, Tri-sodium citrate, Westergren Method, Comparison

scholarly journals A comparison of erythrocyte sedimentation rates of bloods anticoagulated with trisodium citrate and EDTA among TB presumptive patients at the University of Gondar comprehensive specialized hospital, northwest Ethiopia

2020 ◽  
Author(s):  
Zegeye Getaneh ◽  
Fekadu Ayelgn ◽  
Geletaw Asemahegn ◽  
Habtamu Geleta ◽  
Aregawi Yalew ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Sodium Citrate ◽  
Trisodium Citrate ◽  
Northwest Ethiopia ◽  
Cross Sectional ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
The Mean ◽  
The University

Abstract Objective: The purpose of this study was comparing the erythrocyte sedimentation rate (ESR) results of trisodium citrate (TSC) and ethylene diamine tetra-acetic acid (EDTA) anticoagulants. A comparative cross-sectional study was conducted at the University of Gondar specialized referral hospital, northwest, Ethiopia. A total of 70 TB presumptive participants were recruited. From each of the 70 participants of the study, 3 and 1.6 ml of blood was collected in EDTA tubes and 0.4 ml of trisodium Citrate anticoagulant containing test tubes, respectively. Results: The mean ± SD values of ESR were 57.9 ± 41.45 mm/hr in EDTA and 50.99 ± 43.5 mm/hr in TSC anticoagulated blood. The mean difference of ESR values between EDTA and TSC blood (6.91 ± 13.66 mm/hr) was statistically significant. The Mean ± SD of ESR values using EDTA and TSC in males were 59.57 ± 42.31 and 53.57 ± 44.61 mm/hr while for females it was 54.71±40.44 and 46.04 ± 41.82 mm/hr, respectively. The study indicated that there was a significant difference between ESR values with EDTA and tri-sodium citrate anticoagulants. Keywords: Erythrocyte sedimentation rate, EDTA, Tri-sodium citrate, Westergren Method, Comparison

scholarly journals A Comparative Study of Erythrocyte Sedimentation Rate using Saline Diluted and Undiluted EDTA with TSC

2021 ◽  
Vol 9 (VI) ◽  
pp. 494-499
Author(s):  
Sruthy Raphel
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Acute Phase Proteins ◽  
Specific Measure ◽  
International Council ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
The Mean ◽  
Edta Blood ◽  
Edta Anticoagulated Blood

Erythrocyte sedimentation rate (ESR) is the rate at which RBC sediment in a period of 1 hr. It is a common Haematology test that is a non-specific measure of inflammation and it became a common screening test worldwide for acute phase proteins and chronic diseases. The International Council for Standardization in Haematology (ICSH) recommended the Westergren method as the method of choice for ESR determination. TSC is almost exclusively used as the diluent of choice for setting up ESR, but some contemporary laboratories have resolved to use Normal saline (NS) as the diluent of choice while other set ESR using EDTA anticoagulated Blood (BLD) without any diluent. The objective of this study is to assess the comparison between saline diluted and undiluted EDTA with TSC as an anticoagulant in ESR detection and to find out any gender wise variations by using these anticoagulants. A total of 50 students were participated in this study. From each of the participants 5 ml of BLD was collected and it is then divided into 3 parts. 1.6 ml BLD to 0.4ml 3.8% TSC tube, 1.6 ml EDTA blood to 0.4 ml NS tube. 3rd tube with 2 ml EDTA BLD and set for ESR and obtained result within 1 hour. The Result is the mean +SD value of ESR were 19.48+5.7 mm/hr. in undiluted EDTA, 15.22+4.6 mm/hr. in saline diluted EDTA & 15.36+4.5 mm/hr. in TSC. The mean difference of ESR value between saline diluted EDTA with TSC BLD was 0 and it with undiluted EDTA was 4 mm /hr. The study indicates that there was a significant difference between ESR value with undiluted EDTA and TSC while diluted EDTA and TSC were there is no significant difference. The mean +SD of ESR value using undiluted, diluted EDTA and TSC in males were 16.20+3.3, 11.05+2.8, 11.29+2.6 and while it for females were 21.69+5.2, 17.36+3.8, 17.45+3.8 respectively. In conclusion, TSC is the best diluent to be used in contemporary lab to set ESR as compared to EDTA BLD. But we can use saline diluted EDTA as an alternative to citrate diluted BLD to set ESR and it also showed there is a gender wise variation in ESR using these anticoagulants.

scholarly journals Is Vision C interchangeable with the modified Westergren method for the erythrocyte sedimentation rate?

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Serpil Erdogan ◽  
Ridvan Firat ◽  
Gamze Avcioglu ◽  
Gulsen Yilmaz ◽  
Ozcan Erel ◽  
...  
Keyword(s):  
Quality Control ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Room Temperature ◽  
Temperature Correction ◽  
Mean Difference ◽  
Automated System ◽  
Cross Sectional ◽  
Erythrocyte Sedimentation ◽  
The Mean

Abstract Objectives As one of the most widely used tests, the erythrocyte sedimentation rate (ESR) is a measurement of sedimentation reaction in blood samples. Although the recommended method for ESR measurement is the Westergren method, this method has some disadvantages in comparison with automated ESR systems. In this cross-sectional study, we aimed to compare Vision c with the Westergren method. Methods The Vision c automated ESR system and the modified Westergren method were compared using K3EDTA-containing tubes and citrated blood tubes from randomly selected 100 patients. Precision, bias, and interference calculations were performed. Results The mean difference between the Vision c (room temperature) and the modified Westergren method was 22.8 ± 26.6 mm/h (95% CI for the mean was 17.50–28.08 mm/h). The mean difference between the Vision c (18 °C) and the modified Westergren method was 27.4 ± 30.6 mm/h (95% CI for the mean was 21.3–33.5 mm/h). The calculated regression analysis equation was “y= 0.263 + 1.053x” and “y= −0.530 + 0.851x” for the Vision c (room temperature) and the Vision c (18 °C), respectively. The imprecision values ranged at 7.55–17.09%. According to our external quality control results, bias was 11.11 and 9.66 for the low- and high-level samples, respectively. Conclusions The Vision c automated ESR system has a comparable analytical performance with the modified Westergren method. The Vision c automated system may be utilized in ESR measurements with quality control evaluations. Nevertheless, temperature correction using Manley’s monogram causes an important negative bias and should be taken into consideration during the evaluation of the Vision c results.

scholarly journals Estimation of Variations in the Erythrocyte Sedimentation Rate in COVID-19 Recovered Patients

2021 ◽  
pp. 413-418
Author(s):  
Manoj Aravindan ◽  
Palati Sinduja ◽  
R. Priyadharshini ◽  
V. Meghashree
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Signs And Symptoms ◽  
Mean Value ◽  
Multiple Organ ◽  
P Value ◽  
Cross Sectional ◽  
Erythrocyte Sedimentation ◽  
The Mean ◽  
Novel Coronavirus

Background: A cluster of patients with pneumonia and severe acute respiratory syndrome developed in Wuhan, China in December 2019, and infection with a novel coronavirus virus called COVID -19 was later verified. Although fever and cough were the most common early signs and symptoms of COVID -19, extrapulmonary symptoms have also been reported. Many studies demonstrate that called COVID -19 swiftly progresses to acute respiratory distress syndrome and even multiple organ dysfunction. Aim:The study aims to evaluate the erythrocyte sedimentation rate count in COVID -19 recovered individuals and compare it with healthy controls. Materials & Methods: A cross-sectional pilot study in 5 healthy COVID -19 uninfected and 5   COVID -19 recovered individuals was conducted, the blood samples were collected and the erythrocyte sedimentation rate was calculated for each set of controls and samples. Statistical analysis was performed using SPSS software. An independent t-test was done to compare the results. Results: The mean value of the control participants was found to be 8.08 ± 4.38 and the mean value of COVID -19 recovered patients was 20.60 ± 1.81. The difference in Erythrocyte Sedimentation Rate (ESR) values between control individuals and COVID -19 recovered patients was statistically significant with p value of less than 0.005. Conclusion: Within the limitations of the study, we conclude that the COVID -19 recovered patients has higher ESR values compared to the healthy uninfected individuals.

Comparison of K2 and K3 EDTA Anticoagulant on Complete Blood Count and Erythrocyte Sedimentation Rate

2021 ◽  
Vol 28 (1) ◽  
pp. 75
Author(s):  
Harida Zahraini ◽  
Yulia Nadar Indrasari ◽  
Hartono Kahar
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Blood Count ◽  
Complete Blood Count ◽  
Rank Test ◽  
Cross Sectional ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
The Difference ◽  
Cross Sectional Design

The use of anticoagulants is one of the important pre-analytic factors in hematological tests. Both dipotassium (K2) andtripotassium (K3) Ethylene Diamine Tetraacetic Acid (EDTA) are widely used anticoagulants. International CouncilStandardization of Hematology (ICSH) and several researchers recommend the use of K2 EDTA due to its less hyperosmolareffect on blood cells compared to K3 EDTA. This study aimed to compare the results of Complete Blood Count (CBC) andErythrocyte Sedimentation Rate (ESR) using anticoagulant K2 EDTA and K3 EDTA. This study was an analytic observationalstudy with a cross-sectional design conducted from April to December 2018. The subject of the study were 103 healthyadults selected by consecutive sampling. Blood samples were collected in both anticoagulant tubes with a volume of 3 mLeach. Samples were tested twice, in the first 0 hours and the next 6 hours using Sysmex XN 1000 and Alifax Roller 20 LC.Kolmogorov-Smirnov test, paired T-test and Wilcoxon rank test were used for statistical analysis. The agreement testbetween both anticoagulants was carried out using the Bland Altman plot for parameters with a significant difference. Therewas a significant difference between both anticoagulants for the parameters of hemoglobin, hematocrit MCV, MCHC, RDW,PDW, MPV, PLC-R, and erythrocyte sedimentation rate in both the first and second tests. The agreement test using the BlandAltman plot showed that the difference in these parameters was within the Limit of Agreement (LOA) range of 95%. Thisstudy showed that there were differences in some parameters of complete blood count and erythrocyte sedimentation ratebetween the two anticoagulants (K2 K3 EDTA), but these differences were within the LOA range.

scholarly journals Variation in Early Inflammatory Marker Testing for Infection-Related Hospitalizations in Children

2020 ◽  
Vol 10 (10) ◽  
pp. 851-858
Author(s):  
Jessica L. Markham ◽  
Cary W. Thurm ◽  
Matt Hall ◽  
Samir S. Shah ◽  
Ricardo Quinonez ◽  
...  
Keyword(s):  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Inflammatory Marker ◽  
Diagnostic Testing ◽  
Health Information System ◽  
C Reactive Protein ◽  
Cross Sectional ◽  
Reactive Protein ◽  
Erythrocyte Sedimentation ◽  
Significant Difference

BACKGROUND AND OBJECTIVES: Inflammatory marker testing in children has been identified as a potential area of overuse. We sought to describe variation in early inflammatory marker (C-reactive protein and erythrocyte sedimentation rate) testing for infection-related hospitalizations across children’s hospitals and to determine its association with length of stay (LOS), 30-day readmission rate, and cost. METHODS: We conducted a cross-sectional study of children aged 0 to 17 years with infection-related hospitalizations using the Pediatric Health Information System. After adjusting for patient characteristics, we examined rates of inflammatory marker testing (C-reactive protein or erythrocyte sedimentation rate) during the first 2 days of hospitalization. We used k-means clustering to assign each hospital to 1 of 3 groups on the basis of similarities in adjusted diagnostic testing rates across 12 infectious conditions. Multivariable regression was used to examine the association between hospital testing group and outcomes. RESULTS: We included 55 771 hospitalizations from 48 hospitals. In 7945 (14.3%), there was inflammatory marker testing in the first 2 days of hospitalization. We observed wide variation in inflammatory marker testing rates across hospitals and infections. Group A hospitals tended to perform more tests than group B or C hospitals (37.4% vs 18.0% vs 10.4%; P < .001) and had the longest adjusted LOS (3.2 vs 2.9 vs 2.8 days; P = .01). There was no significant difference in adjusted 30-day readmission rates or costs. CONCLUSIONS: Inflammatory marker testing varied widely across hospitals. Hospitals with higher inflammatory testing for one infection tend to test more frequently for other infections and have longer LOS, suggesting opportunities for diagnostic stewardship.

scholarly journals Emotional Bonding and Sexual Activity During COVID-19 Lockdown: A Cross-National Pilot Study

2021 ◽  
pp. 263183182110274
Author(s):  
Deblina Roy ◽  
Sujita Kumar Kar ◽  
SM Yasir Arafat ◽  
Pawan Sharma ◽  
Russell Kabir
Keyword(s):  
Online Survey ◽  
Sampling Technique ◽  
Sexual Relationships ◽  
Sexually Active ◽  
Online Questionnaire ◽  
Cross Sectional ◽  
Emotional Bonding ◽  
Significant Difference ◽  
The Mean ◽  
Study Participants

Background: The COVID-19 pandemic and lockdown measures have affected the sexuality and emotional bonding among the couple across the world. Objectives: We aimed to assess the effects of the COVID-19 pandemic and lockdown on the married people’s emotional bonding and sexual relationships in 3 south Asian counties (Bangladesh, India, and Nepal). Methods: A cross-sectional online survey was conducted among Bangladesh, India, and Nepal residents from April 3 to April 15, 2020. The survey was designed in English. The participants were selected through convenience sampling technique, the link of the online questionnaire was shared with the participants. Only participants older than 18 years and above, married, and living with their spouses were included in the study. Results: A total number of 120 respondents were included finally for analysis from the participating countries (India, Nepal, and Bangladesh). The mean age of the participants was 35.42 (±5.73) years; the majority were males under the age of 40 years and had completed postgraduation as their qualification. Among the study participants, more than half (53.8%) of the women reported being sexually active during the lockdown, whereas 41% of the men reported being sexually active. Among the sexually active participants, most women (57.7%) reported that they perceived positive emotional bonding with their partners. Nevertheless, there was no significant difference observed when compared with men. There are variations in responses. However, no significant association was identified. Conclusion: There are a few insights from the study, that is, there was no significant difference found in almost 3 countries in emotional intimacy. There had been a trend that there is improved emotional bonding with their partners, although no significant difference was observed.

Association of Inflammation With Markers of Atherogenicity in Subjects With New-Onset Atria Fibrillation in a Nigerian Community

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S80-S80
Author(s):  
Constance Nwadike ◽  
Amarachi Nwakamma ◽  
Harrison Nwanjo ◽  
Denis Nwosu ◽  
Roy Ezekwe
Keyword(s):  
Platelet Count ◽  
Prothrombin Time ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Serum Lipids ◽  
High Density Lipoproteins ◽  
Mean Values ◽  
Erythrocyte Sedimentation ◽  
The Mean ◽  
New Onset

Abstract Objectives The evidence supporting the role of circulating markers of inflammation in the pathogenesis of atria fibrillation remains controversial. This study investigated the levels of serum lipids, C-reactive proteins, erythrocyte sedimentation rate, prothrombin time, and platelet count in subjects with new-onset atria fibrillation (NAF), but with no established cardiac disease, in a Nigerian community. Methods The case control study involved a total of 200 subjects: 110 subjects with NAF and 90 apparently healthy subjects without AF, as control. AF was confirmed by a 12-lead electrocardiogram (ECG), while excluding subjects with high risk. Blood samples from the subjects were analyzed for the following parameters: C-reactive proteins using ELISA method and serum lipids using an enzymatic, colorimetric method; erythrocyte sedimentation rate, by Westergreen method; platelet count using a Midray hematology auto analyzer; and the prothrombin time using a tissue thromboplastin method. Data collected from the result were analyzed using SPSS version 15. Results There was a significant increase in the levels of C-reactive proteins and ESR in subjects with NAF when compared with the control subjects. A significant increase was observed in the mean values of total cholesterol and low-density lipoproteins in subjects with NAF, while that of high-density lipoproteins was lower in NAF than in controls. There was no significant difference in the mean values of triglyceride in NAF and the control. Results also showed significantly reduced prothrombin time, with more platelet count in subjects with NAF. The result demonstrated that females (56.3%) had greater risk of developing AF than males (45.8%), while incidence of AF was observed to increase with age. Conclusion The results of the markers assayed suggest marked dyslipidemia, inflammation, and thrombogenesis in subjects with new-onset AF. Hence dyslipidemia and inflammation play critical roles in the pathogenesis of atria fibrillation.

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