scholarly journals Study of Genetic Diversity of the Aphid Predator Hippodamia variegata (Goeze) in Different Areas of Syria Using SRAP markers

2021 ◽  
Vol 39 (3) ◽  
pp. 189-196
Author(s):  
Alaa Mohamad ◽  
◽  
Abdelnabi Basheer ◽  
Salam Lawand ◽  
◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Biological Control ◽  
Phylogenetic Tree ◽  
Genetic Distance ◽  
Geographical Distribution ◽  
Research Center ◽  
Srap Markers ◽  
Polymorphism Rate ◽  
Hippodamia Variegata

This study was conducted in 2020 at the Laboratory of Biotechnology, Agronomy Department and Research Center for Biological Control Studies, Faculty of Agriculture, Damascus University. This study aimed to determine the genetic diversity of the variegated ladybug aphid predator Hippodamia variegata (Goeze) (Coleoptera: Coccinellidae) collected from different regions in Syria using the SRAP technique (Sequence-related Amplified polymorphism). The number of amplified fragments was 101 bands, 99 of which were polymorphic with a polymorphism rate of 97.99%. The number of polymorphic fragments ranged between two bands by using the primers pair SRAP-12 and 13 bands by using the primers pair SRAP-1, with an average of 6.6 bands for each primers pair. The lowest polymorphism rate was 83.33% for primers pairs SRAP-4 and SRAP-9, whereas the highest polymorphism rate was 100% for the rest of the primers pairs. The results obtained on the degree of genetic diversity of the predator's individuals H. variegata showed that the highest value for percent disagreement values was 0.904 between samples from Damascus and Latakia (Fedio) and Homs and Latakia (Fedio), whereas the lowest value for percent disagreement values was 0.271 between samples from Damascus and Sweida. The phylogenetic tree showed that the studied populations were divided according to their geographical distribution into two main groups; the first group included samples collected from the regions of Damascus, Sweida, and Homs with a genetic distance of 15.51, whereas the second group included samples collected from the regions of Tartous, Jabla (Beit Yashout), and Lattakia (Fedio) with a genetic distance of 11.08. This is the first study to determine genetic diversity of H. variegata in Syria. Keywords: Genetic diversity, predator, Hippodamia variegata, SRAP, Syria

Evaluation of Genetic Diversity in Castor (Ricinus communis L.) using RAPD Markes

2011 ◽  
Vol 343-344 ◽  
pp. 981-987
Author(s):  
Feng Juan Li ◽  
Chang Lu Wang ◽  
Dong He ◽  
Ya Qiong Liu ◽  
Mian Hua Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Tree ◽  
Genetic Distance ◽  
Oil Content ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Ricinus Communis ◽  
Major Group ◽  
Ricinus Communis L ◽  
Polymorphic Bands

RAPD markers are used to study the genetic diversity of the main planting on 37 castor varieties widely cultivated in china according to the oil content and other characteristic of different castor varieties. Genetic distance of 37 Chinese castor varieties is studied by RAPD markers analysis. RAPD analysis shows that a total of 122 bands are amplified from random primers of 20 S series, including 71 polymorphic bands with polymorphic rate of 58.20%. 37 castor beans are divided into four major groups in the phylogenetic tree. One castor germplasm is included in1, 2, 3 groups respectively, and two sub-groups are included in the 4 major group.

scholarly journals KERAGAMAN GENETIK 22 AKSESI PADI LOKAL TORAJA UTARA BERBASIS MARKA SIMPLE SEQUENCE REPEATS (SSR)

2019 ◽  
Vol 5 (2) ◽  
pp. 230
Author(s):  
Holy Ekklesia Ladjao ◽  
Rinaldi Sjahril ◽  
Muh. Riadi
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Ssr Markers ◽  
Simple Sequence Repeats ◽  
Genetic Similarity ◽  
Rice Accession ◽  
Polymorphism Rate ◽  
Simple Sequence

Genetic Diversity of 22 Local Rice Accessions from North Toraja Based on Simple Sequence Repeats (SSR) MarkersABSTRACTOne way to explore the potential of local rice is by the characterization that could obtain genetic diversity of that plants. The aim of this study was to obtain the genetic diversity of 22 local rice accession from North Toraja. Twenty-two of local rice accessions from North Toraja were characterized by 30 SSR markers and using NTSYS pc 2.1 program to analyze genetic diversity. The results showed that twenty-six SSR markers that had been analyzed produced some alelles with a size between 106.75-311 bp, the average number of alleles were 3 and the polymorphism rate was 0.53. On coefficient genetic similarity at 0.38, the population formed three clusters. Cluster I and II were dominated by rice that had no hair on the tip of the grain and cluster III were dominated by rice that had hair on the tip of the grain. There were 105 opportunities to crossing between accessions when the genetic distance was above 0.7.Keywords: genetic diversity, local rice, North Toraja, polymorphism rate, SSR markers ABSTRAKSalah satu cara untuk menggali potensi padi lokal adalah dengan karakterisasi. Dengan adanya kegiatan karakterisasi tersebut maka dapat diketahui bagaimana keragaman genetik dari suatu tanaman. Penelitian ini bertujuan untuk mengetahui keragaman genetik dari 22 aksesi padi lokal Toraja Utara. Duapuluh dua aksesi padi lokal Toraja Utara dikarakterisasi menggunakan 30 marka SSR dan dianalisis keragaman genetiknya menggunakan program NTSYS pc 2.1. Hasil penelitian menunjukkan bahwa duapuluh enam marka SSR yang dianalisis memiliki kisaran ukuran alel antara 106.75-311 bp, dengan jumlah alel rata-rata 3 dan tingkat polimorfisme sebesar 0,53. Koefisien kemiripan genetik 0,38 dan terbentuk 3 klaster. Pada klaster I dan klaster II didominasi oleh padi yang tidak memiliki rambut pada ujung gabahnya, dan pada klaster III didominasi oleh padi yang memiliki rambut pada ujung gabahnya. Selain itu, pada jarak genetik diatas 0,7 terdapat 105 peluang persilangan.Kata Kunci: keragaman genetik, marka SSR, padi lokal, tingkat polimorfisme, Toraja Utara

scholarly journals Genetic diversity of Sragen Black Cattle based on D-Loop gene sequencing analysis

2020 ◽  
Vol 18 (2) ◽  
pp. 124
Author(s):  
Rahayu Kusumaningrum ◽  
Sutopo Sutopo ◽  
Edy Kurnianto
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Tree ◽  
Genetic Distance ◽  
Genetic Relationship ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Evolutionary Genetics ◽  
Sequencing Analysis ◽  
Dna Sequence Polymorphism ◽  
D Loop

<p class="MDPI17abstract"><strong>Objective: </strong>The objective of this study was to investigate the genetic diversity of Sragen Black Cattle based on D-loop sequences analysis.</p><p class="MDPI17abstract"><strong>Methods: </strong>A total of 25 blood samples belonged to Sragen Black Cattle that had no genetic relationship within sample. The DNA genome was extracted based on the manufacturer’s standard protocol using gSYNC DNA Mini Kit (Geneaid Biotech Ltd.). D-loop gene was amplified using specific primer forward: 5’- TAGTGCTAATACCAACGGCC-3’ and reverse: 5’- AGGCATTTTGAGTGCCTTGC-3’ and then was sequenced. The sequencing result was aligned and analyzed by Molecular Evolutionary Genetics Analysis (MEGA) version 6.0 to reveal genetic distance and phylogenetic tree. Genetic diversity and haplotype were analysed by DNA Sequence Polymorphism (DnaSp) v6.12.03.<strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong>The results revealed that there were 11 haplotypes with Pi = 0.00675±0.00201 and Hd = 0.767±0.086. Sragen Black Cattle was divided by two cluster in phylogenetic tree with average of genetic distance was 0.0032.<strong></strong></p><p class="MDPI17abstract"><strong>Conclusions: </strong>In conclusion, all of Sragen Black Cattle are on the same cluster and have closer genetic relationship to Bos indicus rather than Bos taurus with similarity level 85.76 % based on BLAST program.</p>

Genetic Diversity and Relationships of 24 Strains of Genus Auricularia (Agaricomycetes) Assessed Using SRAP Markers

2016 ◽  
Vol 18 (10) ◽  
pp. 945-954
Author(s):  
Ping Du ◽  
Tian-Xu Cao ◽  
Bao-Kai Cui ◽  
Yuan Yuan ◽  
Yu-Cheng Dai
Keyword(s):  
Genetic Diversity ◽  
Srap Markers

Genetic diversity in fiveMacrobrachiumhainanensepopulations using SRAP markers

2010 ◽  
Vol 18 (2) ◽  
pp. 150
Author(s):  
Fu Hongtuo ◽  
Qiao Hui ◽  
Yao Jianhua ◽  
Gong Yongsheng ◽  
Wu Yan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Srap Markers

ANALYSIS OF GENETIC DIVERSITY OF FOUR TAKIFUGU OBSCURUS POPULATIONS USING SRAP MARKERS

2013 ◽  
Vol 36 (5) ◽  
pp. 858-864
Author(s):  
Chang-Hong CHENG ◽  
Min-Ying ZHANG ◽  
Kai LIU ◽  
Dong-Po XU ◽  
Jin-Rong DUAN ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Srap Markers ◽  
Takifugu Obscurus

Reduce Disease Burden of Human Schistosomiasis in Asia Through Biological Control

2020 ◽  
Vol 20 (12) ◽  
pp. 1118-1132
Author(s):  
Sidra Saleem ◽  
Haroon Ahmed ◽  
Tooba Siddiqui ◽  
Seyma Gunyakti Kilinc ◽  
Aisha Khan ◽  
...  
Keyword(s):  
Biological Control ◽  
Geographical Distribution ◽  
Disease Burden ◽  
Management Practices ◽  
Control Strategies ◽  
Parasitic Disease ◽  
Asian Countries ◽  
Neglected Disease ◽  
Current Review ◽  
Near Future

Schistosomiasis is a chronic parasitic disease caused by a trematode blood fluke of the genus Schistosoma that belongs to the Schistosomatidae family. It is a neglected disease in different regions of Asia. In this review, 218 articles (between 2000 and 2017) related to the topic were collected from PubMed and Google scholar and reviewed. After thoroughly reading collected articles, due to irrelevant topic requirements, 94 articles were excluded. Articles that have data associated with Asian regions are considered. In Asia, the disease is prevalent in China, Philippines, Indonesia, Yemen, Nepal and Laos, etc. While in Pakistan, India and Bangladesh, the disease is not endemic and very few cases were reported. The disease was eliminated from Japan and Iran. The current review highlights the geographical distribution among Asian countries, transmission patterns, diagnosis, control strategies based on the use of anthelmintic plants and management practices implemented in Asia for the control of schistosomiasis. However, new implementations to treat schistosomiasis in humans should be proved to eliminate the disease finally in the future. This review emphasizes the biological control of schistosomiasis for the eradication of the disease from Asia in the near future.

scholarly journals Unveiling a unique genetic diversity of cultivated Coffea arabica L. in its main domestication center: Yemen

2021 ◽  
Author(s):  
C. Montagnon ◽  
A. Mahyoub ◽  
W. Solano ◽  
F. Sheibani
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Coffea Arabica ◽  
Genetic Improvement ◽  
The World ◽  
Genetic Clusters ◽  
Domestication Process ◽  
Almost All ◽  
Coffea Arabica L ◽  
Cup Quality

AbstractWhilst it is established that almost all cultivated coffee (Coffea arabica L.) varieties originated in Yemen after some coffee seeds were introduced into Yemen from neighboring Ethiopia, the actual coffee genetic diversity in Yemen and its significance to the coffee world had never been explored. We observed five genetic clusters. The first cluster, which we named the Ethiopian-Only (EO) cluster, was made up exclusively of the Ethiopian accessions. This cluster was clearly separated from the Yemen and cultivated varieties clusters, hence confirming the genetic distance between wild Ethiopian accessions and coffee cultivated varieties around the world. The second cluster, which we named the SL-17 cluster, was a small cluster of cultivated worldwide varieties and included no Yemen samples. Two other clusters were made up of worldwide varieties and Yemen samples. We named these the Yemen Typica-Bourbon cluster and the Yemen SL-34 cluster. Finally, we observed one cluster that was unique to Yemen and was not related to any known cultivated varieties and not even to any known Ethiopian accession: we name this cluster the New-Yemen cluster. We discuss the consequences of these findings and their potential to pave the way for further comprehensive genetic improvement projects for the identification of major resilience/adaptation and cup quality genes that have been shaped through the domestication process of C. arabica.

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