Primary Metabolite Profiling and Potential Antioxidants Activity from Fagonia cretica (Stem and Roots)

Many carbonic compounds are found in medicinal plants, which work as a drug for human body. Fagonia cretica (Linn.) is most valuable therapeutic medicinal plant, commonly known as Dhamasa and belong to Zygophyllaceae family. It is used as: piles, urinary disorders, dysentery, stomach ache, typhoid, cancer and as a blood purifier. Fagonia cretica is a small spiny under-shrub, found in North West India, Punjab, Deccan and Afghanistan. The present study deals in Primary Metabolite Profiling and Potential Antioxidant activity from root and stems of experiment plants. In vitro study of Primary Metabolites such as carbohydrates (starch & total soluble sugar), proteins, phenols and lipids, primary metabolites are a good source of nutrient and energy for cells and Antioxidant activities like: catalase, peroxidase, lipid peroxidase and FRAP by crude extract of various plant parts (root and stems) was investigated of Fagonia cretica. Antioxidants fight as anti cancer agents in human beings. Keywords: Fagonia cretica; Primary metabolites; UV spectroscopy; Antioxidants.

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Primary Metabolites Profiling of Vetiveria Lawsonii from Leaf and Root

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i4-a.3497 ◽

2019 ◽

Vol 9 (4-A) ◽

pp. 373-375

Author(s):

Anuradha Gauttam ◽

Nakuleshwer Dutt Jasuja ◽

Rakesh Kumar

Keyword(s):

Medicinal Plant ◽

Soluble Sugar ◽

Soxhlet Extraction ◽

High Concentration ◽

Primary Metabolites ◽

Total Soluble Sugar ◽

Medicinal Value ◽

Quantitative Extraction ◽

Poaceae Family ◽

Ethanol Extracts

Various traditional systems of medicine enlightened the importance of Indian plants to have a great medicinal value. The present study was aimed to evaluate the Primary Metabolites study of Vetiveria lawsonii, belong to Poaceae family. Extracts were prepared in methanol, ethanol by Soxhlet extraction. Quantitative extraction of preliminary phytochemicals investigation revealed the presence of Carbohydrates (Starch and Total Soluble Sugar), Lipid, Proteins, and Phenol by using UV spectrometer. Experimental medicinal plant Vetiveria lawsonii are showing high concentration of primary metabolites. Hence, we can conclude that the methanol and ethanol extracts of Vetiveria lawsonii was possess primary metabolites. Keywords: - Vetiveria lawsonii; Primary Metabolites.

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METABOLITE PROFILING AND IN VITRO ASSESSMENT OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF LICHEN RAMALINA INFLATA

International Research Journal of Pharmacy ◽

10.7897/2230-8407.0712159 ◽

2017 ◽

Vol 7 (12) ◽

pp. 132-138

Author(s):

Ayyappadasan Ganesan ◽

Deepak Kumar Purushothaman ◽

Uthira Muralitharan ◽

Rubavathi Subbaiyan

Keyword(s):

Metabolite Profiling ◽

Antioxidant Activities ◽

In Vitro Assessment

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Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence Lycoris sprengeri Bulblet Regeneration In Vitro

International Journal of Molecular Sciences ◽

10.3390/ijms222111890 ◽

2021 ◽

Vol 22 (21) ◽

pp. 11890

Author(s):

Ziming Ren ◽

Yunchen Xu ◽

Xuesi Lvy ◽

Dong Zhang ◽

Cong Gao ◽

...

Keyword(s):

In Vitro Regeneration ◽

Expression Patterns ◽

Soluble Sugar ◽

Cleavage Pattern ◽

Regeneration System ◽

Total Soluble Sugar ◽

Bulb Yield ◽

Sucrose Cleavage

Bulblet formation and development determine the quantitative and qualitative traits, respectively, of bulb yield for most flowering bulbs. For Lycoris species, however, the underlying molecular mechanism remains elusive. Here, clonal bulblets of Lycoris sprengeri (Ls) derived from the same probulb were used as explants to establish efficient and inefficient in vitro regeneration systems by adjusting the 6-benzyladenine (BA) concentrations in media. BA application did not change the biological processes among groups but led to earlier decreases in sucrose and total soluble sugar (TSS) contents. Correlation analyses showed that the BA treatments changed the interaction between carbohydrate and endogenous hormone contents during bulblet regeneration. We found that two sucrose degradation enzyme-related genes, cell wall invertase (CWIN) and sucrose synthase, exhibited exactly opposite expression patterns during the competence stage. In addition, the regeneration system that obtained more bulblets showed significantly higher expression of LsCWIN2 than those that obtained fewer bulblets. Our data demonstrate the essential role of BA in accelerating sucrose degradation and the selection of a dominant sucrose cleavage pattern at the competence stage of in vitro bulblet regeneration. We propose that a relatively active CWIN-catalyzed pathway at the competence stage might promote bulblet regeneration, thus influencing bulb yield.

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Phytochemical Analysis and Antioxidant Potential of Rhizome Extracts of Curcuma amada Roxb and Curcuma caesia Roxb

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i5.3609 ◽

2019 ◽

Vol 9 (5) ◽

pp. 123-126

Author(s):

Mamta Yadav ◽

K. Kalyan Saravanan

Keyword(s):

Manufacturing Industry ◽

Antioxidant Activities ◽

Perennial Herb ◽

Assay Method ◽

Phytochemical Analysis ◽

Human Beings ◽

Dpph Assay ◽

Test Protocol ◽

Curcuma Amada

Plants have served human beings as a natural source for treatments and therapies from ancient times, amongst them medicinal herbs have gain attention because of its wide use and less side effects. In the recent years plant research has increased throughout the world and a huge amount of evidences have been collected to show immense potential of medicinal plants used in various traditional systems. Curcuma amada Roxb (C. amada, Zingiberaceae) is a perennial, rhizomatous, aromatic herb commonly known as Amada or Amahaldi or mango ginger due to the raw mango-like aroma of the rhizome. It is used medicinally as a coolant, astringent and to promote digestion. Curcuma caesia Roxb (C. caesia, Zingiberacea) is a perennial herb with bluish black rhizomes commonly known as black turmeric and are traditionally used in treatment of various ailments and metabolic disorders like leukoderma, asthma, tumours, piles, bronchitis, etc. in Indian system of medicine. The aim of the present study was to evaluate in vitro antioxidant activities and qualitative and quantitative phytochemical analysis of rhizome of C. amada and C. caesia collected from Bhopal region of Madhya Pradesh. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. The in vitro antioxidant activity of methanolic extract of the rhizome was assessed against DPPH assay method using standard protocols. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, saponins, alkaloids. The total flavonoids content of methanolic rhizomes extract of C. caesia and C. amada was found to be 2.752 and 2.920 mg/100mg respectively. The activities of methanolic rhizomes extract against DPPH assay method were concentration dependent with IC 50 values of ascorbic acid and extracts 14.11 and 170.81, 63.69μg/ml respectively. The diverse array of phytochemicals present in the plant thus suggests its therapeutic potentials which may be explored in drug manufacturing industry as well as in traditional medicine. Keywords: Curcuma amada, Curcuma caesia, Zingiberacea, Phytochemical analysis, Antioxidant

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Study on the in vitro development of Chrysanthymum indicum L. shoots in the salinity stress

Science and Technology Development Journal - Natural Sciences ◽

10.32508/stdjns.v2i4.804 ◽

2019 ◽

Vol 2 (4) ◽

pp. 5-12

Author(s):

Thang Thanh Tran ◽

Trinh Thi Diem Phan ◽

Huong Thanh Tran

Keyword(s):

Salinity Stress ◽

Stress Condition ◽

Starch Content ◽

Sugar Content ◽

Soluble Sugar ◽

Total Soluble Sugar ◽

Chrysanthemum Indicum ◽

Parenchymal Cells ◽

Vitro Development

In this study, NaCl at varrious concentrations of 4 – 10 g/L was used to investigate the salt tolerance of in vitro shoot cuttings of Chrysanthemum indicum. Morphological, physiological and biochemical changes during the response of shoot cuttings in the salinity stress were analyzed. NaCl at 6 g/L reduced the development of shoot cuttings. Under salinity stress conditions, there have just a little reduction of the chloroplast in parenchymal cells near the midrib of leaf before they turn brown and die. Besides, carotenoid, starch content, and photosynthesis intensity were decreased. In contrast, respiration rate, proline and total soluble sugar content, and the activity of IAA and gibberellin were strongly increased. The application of IAA 0.25 mg/L, zeatin 0.1 mg/L and GA3 0.1 mg/L improved the shoot development in the salinity stress condition. Shoots in MS medium supplemented with BA 0.2 mg/L, NAA 2 mg/L and NaCl 6 g/L grow better in salinity stress condition.

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Micropropagation, metabolite profiling, antioxidant activities and chromatographic determination of bioactive molecules across in vitro conditions and subsequent field cultivation stages of ‘Shampoo Ginger’ (Zingiber zerumbet L. Roscoe ex Sm)

Biocatalysis and Agricultural Biotechnology ◽

10.1016/j.bcab.2018.07.015 ◽

2018 ◽

Vol 16 ◽

pp. 79-89 ◽

Cited By ~ 2

Author(s):

Jaykumar J. Chavan ◽

Parthraj R. Kshirsagar ◽

Sandeep R. Pai ◽

Nilesh V. Pawar

Keyword(s):

Metabolite Profiling ◽

Antioxidant Activities ◽

Chromatographic Determination ◽

Bioactive Molecules ◽

Zingiber Zerumbet

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Effects of contrasting shade treatments on the carbon production and antioxidant activities of soybean plants

Functional Plant Biology ◽

10.1071/fp19213 ◽

2020 ◽

Vol 47 (4) ◽

pp. 342 ◽

Cited By ~ 3

Author(s):

Muhammad Ali Raza ◽

Ling Yang Feng ◽

Nasir Iqbal ◽

Imran Khan ◽

Tehseen Ahmad Meraj ◽

...

Keyword(s):

Antioxidant Activities ◽

Soluble Sugar ◽

Photosynthetic Characteristics ◽

Total Soluble Protein ◽

Protein Accumulation ◽

Total Soluble Sugar ◽

Relay Intercropping ◽

Soybean Plants ◽

Normal Light ◽

Soybean Leaves

In China, maize-soybean relay-intercropping system follow the two main planting-patterns: (i) traditional relay-intercropping; maize-soybean equal row planting, where soybean experience severe maize shading on both sides of plants, and (ii) modern relay-intercropping; narrow-wide row planting, in this new planting pattern only one side of soybean leaves suffer from maize shading. Therefore, in this study, changes in morphological traits, cytochrome content, photosynthetic characteristics, carbon status, and the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were investigated at 30 days after treatment (DAT) in shade-tolerant soybean variety Nandou-12 subjected to three different types of shading conditions; normal light (NL, all trifoliate-leaves of soybean plants were under normal light); unilateral shade (US, all right-side trifoliate-leaves of soybean plants from top to bottom were under shade while all the left-side of trifoliate-leaves from top to bottom were in normal light); bilateral shade (BS, all trifoliate-leaves of soybean plants were under complete shade). Compared with BS, US conditions decreased plant height and increased stem diameter, leaf area, and biomass at 30 DAT. Biomass distribution rates to stem, petiole and leaves, and photosynthetic characteristics were markedly improved by the US at all sampling stages, which proved to be a better growing condition than BS with respect to shade tolerance. The enhanced net photosynthesis and transpiration rates in the left-side leaves (LS) of soybean plants in US, when compared with the LS in BS, allowed them to produce higher total soluble sugar (by 70%) and total soluble protein (by 17%) at 30 DAT which reduce the adverse effects of shading at right-side leaves (RS) of the soybean plants. Similarly, soybean leaves under US accumulated higher proline content in US than the leaves of BS plants. Soybean leaves grown in shading conditions (LS and RS of BS and RS of US) developed antioxidative defence-mechanisms, including the accelerated activities of SOD, POD, APX, and CAT. Comparatively, soybean leaves in US displayed lower activity levels of the antioxidative enzymes than the leaves of BS plants, showing that soybean plants experienced less shade stress in US as compared with BS treatment. Overall, these results indicate that the association of improved photosynthetic characteristics, sugar and protein accumulation and optimum antioxidative defences could be an effective approach for growing soybean in intercropping environments.

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Morphological and Biochemical Responses to Gibberellic Acid in Magnolia × ‘Spectrum’ in Vitro

Acta Biologica Cracoviensia s Botanica ◽

10.1515/abcsb-2016-0010 ◽

2016 ◽

Vol 58 (1) ◽

pp. 103-111 ◽

Cited By ~ 5

Author(s):

Agnieszka Wojtania ◽

Edyta Skrzypek ◽

Eleonora Gabryszewska

Keyword(s):

Gibberellic Acid ◽

Sugar Content ◽

Soluble Sugar ◽

Shoot Formation ◽

Antioxidant Enzyme Activities ◽

Axillary Shoot ◽

Soluble Sugar Content ◽

Total Soluble Sugar ◽

Leaf Formation

AbstractThe total soluble sugar content and antioxidant enzyme activities were studied for the first time during axillary shoot formation inMagnolia× ‘Spectrum’ in vitro in response to BAP (0.3 mg l−1), different levels of gibberellic acid (GA3; 0.0, 0.1, 0.5, 1.0 mg l−1), sucrose (20 and 30 g l−1) and nitrogen salts (KNO3/NH4NO3; 100/100% and 75/50% relative to MS medium). Among various GA3and sucrose/nitrogen salts ratios, the most effective axillary multiplication (5.9 shoots/explant) and leaf formation (25.7 leaves per multiplied clumps) were obtained after addition of GA3at 0.1 mg l−1to a BAP medium containing 20 g l−1sucrose and reduced levels of nitrogen salts (75% KNO3and 50% NH4NO3). The addition of GA3to the BAP medium enhanced shoot formation by 36% and leaf formation by 27%. The highest shoot formation capacity ofM.× ‘Spectrum’ in vitro coincided with enhanced levels of soluble sugar and peroxidase (POD) activity. Increasing GA3concentration from 0.1 to 1.0 mg l−1in the above medium resulted in inhibition of shoot and leaf formation and a decrease in the soluble sugar content. The influence of GA3on the activities of catalase (CAT) and POD depended on its concentration and the levels of sucrose and nitrogen salts in the medium. The highest increase in CAT and POD activities, that coincided with the enhanced shoot formation capacity ofM.× ‘Spectrum’ in vitro, was observed after addition of GA3to the medium containing high levels of sucrose and nitrogen salts.

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