Micropropagation, metabolite profiling, antioxidant activities and chromatographic determination of bioactive molecules across in vitro conditions and subsequent field cultivation stages of ‘Shampoo Ginger’ (Zingiber zerumbet L. Roscoe ex Sm)

Osteoarthritis (OA) is a disease associated to age or conditions that precipitate aging of articular cartilage, a post-mitotic tissue that remains functional until the failure of major homeostatic mechanisms. OA severely impacts the national health system costs and patients’ quality of life because of pain and disability. It is a whole-joint disease sustained by inflammatory and oxidative signaling pathways and marked epigenetic changes responsible for catabolism of the cartilage extracellular matrix. OA usually progresses until its severity requires joint arthroplasty. To delay this progression and to improve symptoms, a wide range of naturally derived compounds have been proposed and are summarized in this review. Preclinical in vitro and in vivo studies have provided proof of principle that many of these nutraceuticals are able to exert pleiotropic and synergistic effects and effectively counteract OA pathogenesis by exerting both anti-inflammatory and antioxidant activities and by tuning major OA-related signaling pathways. The latter are the basis for the nutrigenomic role played by some of these compounds, given the marked changes in the transcriptome, miRNome, and methylome. Ongoing and future clinical trials will hopefully confirm the disease-modifying ability of these bioactive molecules in OA patients.

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Liquid Chromatographic Determination of Dexamethasone and Fluoroquinolones; in vitro Study

South African Journal of Chemistry ◽

10.17159/0379-4350/2019/v72a16 ◽

2019 ◽

Vol 72 ◽

pp. 130-135 ◽

Cited By ~ 1

Author(s):

Nawab Sher ◽

Nasreen Fatima ◽

Shahnaz Perveen ◽

Farhan Ahmed Siddiqui

Keyword(s):

In Vitro Study ◽

Chromatographic Determination ◽

Vitro Study ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic

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Chemical Profiling of Polysaccharides Present in Peels of Citrus limetta and Bioassay based Screening of in vitro Antioxidant Activities

Asian Journal of Chemistry ◽

10.14233/ajchem.2020.22798 ◽

2020 ◽

Vol 32 (9) ◽

pp. 2308-2314

Author(s):

DIPAN ADHIKARI ◽

TUHIN GHOSH

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Low Cost ◽

Standard Procedure ◽

Bioactive Molecules ◽

Size Exclusion ◽

Chemical Compositions ◽

Antioxidant Power ◽

Citrus Limetta

In this analyses, the chemical compositions of polysaccharides isolated from the peels of Citrus limetta had been studied and discussed its antioxidant activity of different active fractions. To emphasize the chemical structure of polysaccharides, a rhamnoglucan polysaccharide was identified with probable ester linked phenolic acid. The sugar composition and purification by size exclusion chromatography (SEC) has been presented. The antioxidant capacities of the extracts prepared from Citrus limetta peel powder were determined using well known in vitro systems and standard procedure for ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH•), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid) (ABTS•+), hydroxyl radical (OH•), nitric oxide (NO) assay, total antioxidant activity (TAA) and metal chelation property. The rhamnoglucan (A) present exhibited the highest bioactivity potentiality succeeded by traces of uronic acid and galactan. From the investigation, it could be emphasized that water extracted polysaccharide, which brings forth potent pharmacological activities figures out the importance as alternative natural compounds as to-be-exploited leads for low-cost sources of efficient bioactive molecules with strong antioxidant activities in different pharmaceutical and cosmoceutical formulations.

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Determination of Flavonoid Contents and Evaluation of in vitro Antioxidant Activities of the Extract of Selected Citrus Fruit Peel

International Journal of Secondary Metabolite ◽

10.21448/ijsm.660578 ◽

2020 ◽

pp. 8-18

Author(s):

Olyad ERBA ◽

Dereje ATOMSA ◽

Meseret CHİMDESSA ◽

Teshome GONFA

Keyword(s):

Antioxidant Activities ◽

Citrus Fruit ◽

Fruit Peel

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Leaf Extracts of Aerva lanata Inhibit the Activities of Type 2 Diabetes-Related Enzymes and Possess Antioxidant Properties

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/3439048 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 5

Author(s):

Musbau Adewunmi Akanji ◽

Samson Olasunkanmi Olukolu ◽

Mutiu Idowu Kazeem

Keyword(s):

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Leaf Extracts ◽

Chemically Induced ◽

Radical Scavenging Ability ◽

Aerva Lanata ◽

Radical Scavenging Properties

The leaves of Aerva lanata are one of the indigenous medicinal plants used in the management of diabetes mellitus and its associated complications in Africa. However, its effect on the activities of diabetes-related enzymes has not been investigated. This study evaluated the in vitro inhibitory effects of different extracts of the A. lanata leaf on the activities of diabetes-related enzymes (α-amylase and α-glucosidase) and chemically induced free radicals. Aqueous, ethanol, and hydroethanol extracts of A. lanata leaves were subjected to a standard enzyme inhibition assay followed by determination of modes of inhibition of the enzymes. The antioxidant activities of the extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). The results obtained showed that the hydroethanol extract of the A. lanata leaf optimally inhibited both α-amylase (IC50: 2.42 mg/mL) and α-glucosidase (IC50: 0.23 mg/mL). The Lineweaver-Burk plot revealed that the mode of inhibition of both enzymes by the hydroethanol extract was uncompetitive. However, the hydroethanol and aqueous extracts displayed the best DPPH and ABTS radical-scavenging ability, respectively. It can be concluded that the A. lanata extract inhibited the activities of both α-amylase and α-glucosidase uncompetitively, which may be attributed to its free radical-scavenging properties and rich phenolic composition.

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CHEMICAL CONSTITUENTS, IN VITRO ANTIOXIDANT ACTIVITY, ORAL ACUTE TOXICITY AND LD50 DETERMINATION OF MORINGA OLEIFERA LEAVES

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i5.17834 ◽

2017 ◽

Vol 9 (5) ◽

pp. 240

Author(s):

Manal Mortady Hamed ◽

Aboelfetoh Mohamed Abdalla ◽

Mosad Ahmed Ghareeb ◽

Said Abdelhalim Saleh

Keyword(s):

Antioxidant Activity ◽

Acute Toxicity ◽

Antioxidant Activities ◽

Total Phenolic ◽

Dry Extract ◽

Phenolic Contents ◽

Moringa Oleifera Leaves ◽

Oral Acute Toxicity

Objective: The objective of this study was undertaken to estimate the total phenolic contents (TPCs), in vitro antioxidant of different solvent extracts of M. oleifera leaves, oral acute toxicity and LD50 determination of the 85% methanolic extract as well as the chromatographic isolation and identification of the extract constituents.Methods: The antioxidant activity of different solvent extracts of Moringa oleifera leaves were estimated using three antioxidant assays and the total phenolic contents (TPCs) were also evaluated using Folin-Ciocalteu’s assay. The n-BuOH extract undergoes further chromatographic isolation owing to the high antioxidant activity using 2, 2'-diphenyl-1-picrylhydrazyl radical (DPPH) method, which resulted in the isolation of seven compounds.Results: The results showed that the TPCs values of the tested extracts were varied from 309.52 to 43.28 mg gallic acid equivalent/g dry extract. The reducing power antioxidant activities (RPAA) were 0.434, 0.402, 0.395, 0.149, 0.143 and 0.124, while the total antioxidant capacity (TAC) values were 316.43, 203.35, 181.56, 86.70, 76.62 and 50.83 mg ascorbic acid equivalent/g dry extract; for n-BuOH, EtOAc, 85% MeOH, H2O, CH2Cl2, and pet. ether extracts, respectively. The oral acute toxicity study of the 85% methanol extracts of M. oleifera and M. peregrina revealed that; their LD50 values were 3458.3 and 4125 mg/kg respectively, thus the two plants could be classified as slightly toxic in the scale of Hodge and Sterner which reflected their nutrient values as edible plants. The isolated compounds were identified on the basis of their 1H and 13C-NMR spectra as; cis-p-coumaric acid 4-O-(2'-O-β-D-apiofuranosyl)-β-D-glucopyranoside (1), chlorogenic acid (2), niazirin (3), 3,4-dihydroxy-β-phenylethoxy-O-α-L-rhamnopyranosyl-(l→2)-α-L-rhamnopyranosyl-(1→3)-4-O-caffeoyl-β-D-glucopyranoside (4), gallic acid (5), taxifolin (6), and benzyl-carbamo-thioethionate (7).Conclusion: The M. oleifera leaves showed promising antioxidant activities and slightly toxic behavior.

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