scholarly journals Investigating the healing effect of the hydroalcoholic extract of pomegranate seed (Punica granatum) on the full thickness wound in rabbit

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Ali Asghar Hemmati ◽  
Iran Rashidi ◽  
Sedigheh Dahanzadeh ◽  
Mahmoud Moeini
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Punica Granatum ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Hydroxyproline Content ◽  
Tissue Samples ◽  
Treatment Groups ◽  
Wound Recovery

Objective: This research designed to investigate the wound healing process with pomegranate hydroalcoholic seed extract (Punica granatum) in comparison with no-treatment, betamethasone, phenytoin and eucerin in rabbits. Methods: The positive group including groups that received phenytoin cream (1%) and topical eucerin, respectively, twice a day to complete wound healing. negative Control group did not obtain any treatment. Treatment groups were received cream of PSE (2, 5,7,10 w/w) in eucerin and 75% w/w as purified extract twice daily. In order to measure the percentage of wound healing, the zone of the wound was evaluated daily. Histological studies were done on the 7th and 15th days of treatment. Next, hydroxyproline content of wounds healed and tensile strength of wound tissue samples were measured. Results: The results demonstrated between PSE treatment groups and eucerin animals were statistically significant aadifferences (P<0.05) in most of the days reviewed. Treatment of Rabbits with 10% PSE had the best results (complete wound recovery in 12 days). Also, this treatment showed higher hydroxyproline content and higher tissue strength. Conclusion: This research reveals that the extract of 10% PSE administrated topically has the proper potential to induce wound recovery in the wound model of rabbits. In addition, 10% PSE accelerates the healing of the wound. Further study needs to clarify the results of this research.

scholarly journals Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Muhammad Reza Setiawan ◽  
Nurdiana Dewi ◽  
Ika Kustiyah Oktaviyanti
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Channa Striata ◽  
Post Hoc

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract hassubstantial properties such as albumin, Zn,Cu, and Feto accelerate wound healing. The purpose of this study is to assessthe effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. Thisstudy was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatmentgroups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, eachtreatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of eachgroup was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant differencebetween negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increasedneocapillaries count in wound healing process.

scholarly journals Effect of haruan (Channa striata) extract on fibroblast cells count in wound healing

2016 ◽  
Vol 1 (2) ◽  
pp. 226
Author(s):  
Ardi Siswanto ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Fibroblast Cells ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The purpose of this study is to assess the effect of haruan extract on wistar rat’s buccal mucosa wound healing on day 3, 5, and 7 histopathologically. This study was experimental with post-test with control group design. Samples were consisted of 4 treatment groups, 25%, 50%, 100% haruan extract treatment groups and negative control treated with<em> aquadest</em> for 7 days. Mean fibroblast cells count reached its peak on day 7. Mean fibroblast cells count in each group was 26.995; 40.500; 49.165; 24.495 respectively. Two way ANOVA and Post Hoc Least Significant Difference tests indicated there was a significant difference (p&lt;0.05) between haruan extract treatment group and negative control. In conclusion, haruan extract treatment significantly increase fibroblast cells count in wound healing process.

scholarly journals Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Muhammad Reza Setiawan ◽  
Nurdiana Dewi ◽  
Ika Kustiyah Oktaviyanti
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference ◽  
Channa Striata ◽  
Post Hoc

<p>Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties suchas albumin, Zn,Cu, andFeto accelerate wound healing. The purposeof this study is to assess the effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. This study was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatment groups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, each treatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of each group was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant difference between negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increased neocapillaries count in wound healing process.</p><p>Haruan adalah ikankhas Kalimantan yangberpotensi mempercepat penyembuhanluka. Ekstrak ikan haruan mengandung zat penting seperti albumin, Zn , Cu, dan Fe untuk mempercepat penyembuhan luka. Penelitian ini dimaksudkan untuk mengetahui pengaruh ekstrak ikan haruan pada proses penyembuhan luka pada mukosa bukal tikus wistar hari ke-1, 3, 5, dan 7 secara histopatologi. Penelitian eksperimental murni dengan pretest-posttest with control group ini, meliputi 4 kelompok,yaitu kelompok hewan yang diberi ekstrak haruan selama7 hari dengan konsentrasi berturut-turut 25%, 50%, 100%, dan kelompok kontrol negatif diberi akuades. Hasilnya menunjukkan bahwa jumlah neokapiler mencapai puncak pada hari ke-5. Rerata jumlah neokapiler pada tiap kelompok berturut adalah 12; 16,5; 18,5; dan 6. Uji Anova 2-jalur dan uji post hoc LSDmenunjukkan adanya perbedaan bermakna antara kontrol negatif dan ekstrak ikan haruan. Berdasarkan hasil penelitian, disimpulkan bahwa pemberian ekstrak ikan haruan secara bermakna meningkatkan jumlah neokapiler pada proses penyembuhan luka.</p>

scholarly journals BIOSYNTHESIS OF pH RESPONSIVE SHAPE MEMORY HYDROGEL AND ITS BIOMEDICAL POTENTIAL

2017 ◽  
Vol 6 (3) ◽  
pp. 946-952
Author(s):  
AHMAD MOHAMMED GUMEL
Keyword(s):  
Wound Healing ◽  
Shape Memory ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Ph Responsive ◽  
Tissue Samples ◽  
Significant Difference ◽  
Biomedical Potential

Multifunctional hydrogels combining the capabilities of cellular pH responsiveness and shape memory, are highly promising for the realization of smart membrane filters, controlled drug released devices, and functional tissue-engineering scaffolds. Free radical biocatalytic polymerization catalyzed by immobilized Candida antarctica lipase B was used to fabricate the pH-responsive and shape memory hydrogel using medium-chain-length poly-3-hydroxyalkanoates-co-polyethylene glycol methacrylate (PHA-PEGMA) as macromer. The accelerative wound healing potential of the biosynthesized smart PHA based hydrogel was evaluated herein. The thermal stability of the macromere highly depends on the PEGMA fraction from 10 to 50% (mass). Similarly, the change in PEGMA fraction was also found to highly influence the hydrogel’s hydration rate (r) from 2.83 x 10-5 to 7.63  x 10-5  mL/s.  The hydrogel’s equilibrium weight swelling ratio (qe), protein release and its diffusion coefficient (Dm) were all found to be pH dependent. For example, increasing the phosphate buffer pH from 2.4 to 13 resulted in increased qe from 2 to 16 corresponding to the enlarging of network pore size (ξ) from 150 to 586 nm.The biomedical potential of the synthesized material based on its accelerative wound healing in rats was observed. Biochemical, histomorphometric and immunohistomorphometric analyses revealed a significant difference in area closure and re-epithelialization on days 7 and 14 in PPH or Intrasite® gel group compared to gum acacia or PEGMA-alone groups. Furthermore, wounds dressed with PPH or Intrasite® gel showed evident collagen deposition, enhanced fibrosis and extensively organized angiogenesis on day 14 compared to the negative control group. The findings suggested that topical application of PPH accelerated the rats’ wound healing process by improving angiogenesis attributed to the increased microvessel density (MVD) and expressions of VEGF-A in tissue samples. Thus, PPH has been demonstrated to be effective in the treatment of cutaneous wounds in rats, and could be a potential novel agent in the management and acceleration of wound healing in humans and animals.

scholarly journals Effect of haruan (channa striata) extract on fibroblast cells count in wound healing

2016 ◽  
Vol 1 (2) ◽  
pp. 89
Author(s):  
Ardi Siswanto ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Fibroblast Cells ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The purpose of this study is to assess the effect of haruan extract on wistar rat’s buccal mucosa wound healing on day 3, 5, and 7 histopathologically. This study was experimental with post-test with control group design. Samples were consisted of 4 treatment groups, 25%, 50%, 100% haruan extract treatment groups and negative control treated with aquadest for 7 days. Mean fibroblast cells count reached its peak on day 7. Mean fibroblast cells count in each group was 26.995; 40.500; 49.165; 24.495 respectively. Two way ANOVA and Post Hoc Least Significant Difference tests indicated there was a significant difference (p<0.05) between haruan extract treatment group and negative control. In conclusion, haruan extract treatment significantly increase fibroblast cells count in wound healing process.

scholarly journals HE EFFECT OF BINJAI LEAVES EXTRACT (Mangifera caesia) ON INCISIONAL BACK WOUND OF MICE (Mus musculus) TO THE NUMBER OF NEUTROPHIL CELLS (Preliminary Study of Drug Preparations for Wound Healing)

2020 ◽  
Vol 5 (1) ◽  
pp. 21
Author(s):  
Desty Ayu Dwiyanti ◽  
Irham Taufiqurrahman ◽  
Didit Aspriyanto
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Group Design ◽  
Inflammatory Phase ◽  
Significant Difference

Background: Ethanol extract of binjai leaves contain bioactive compounds in the form of flavonoid and saponin which is potential as anti-inflammatory, so it will be able to control the wound healing process. Binjai leaves extract can affect the number of neutrophil on inflammatory phase, as it is characterized by neutrophil cells infiltration to the wound tissue, so its number will increase at the beginning of inflammatory phase and decrease on the 3rd day. Objective: To prove the effect of binjai leaves extract in 1 mg/mL, 2 mg/mL, and 3 mg/mL which given topically to the number of neutrophil in day 1, 3, and 5 of wound incision on the back of male mice. Methods: The study design was a pure laboratory experimental study with post-test only with control group design which involved 36 mice divided into 4 groups: negative control group design which was given BR2 feed, treatment group which was given binjai leaves extract in 1 mg/mL, 2 mg/mL, and 3 mg/mL. Results: The result of One-way ANOVA showed that there was significant difference of all groups in day 1 (p=0.000), day 3 (p=0.001), and day 5 (p=0.000). Post-Hoc LSD test showed that there was significant difference (p<0.05) between the negative control group and treatment groups. Conclusion: Binjai leaves extract has an effect to increase the number of neutrophil in day 1 and decrease the number of neutrophil in day 3 and 5 on the healing of back wound incision in mice.

scholarly journals Althaea officinalis extracts heal skin wounds in second- and third-degree burns in mice

2021 ◽  
Author(s):  
Ali Mohammad Ranjbar ◽  
Arefeh Sojaeemehr ◽  
Mohsen Zabihi ◽  
Fatemeh Shishehbor ◽  
Vahid Ramezani
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Metal Plate ◽  
Control Group ◽  
Wound Healing Process ◽  
Aqueous Extracts ◽  
Treatment Groups ◽  
Iranian Traditional Medicine ◽  
Althaea Officinalis ◽  
Skin Healing

Abstract Background and Purpose: Burns are one of the most common injuries. There are few medications to repair skin in burns. Although antibacterial drugs such as topical silver sulfadiazine may prevent wound infections, they usually can’t attenuate skin healing. Marshmallow is used in Iranian traditional medicine to improve skin burns. We aimed to evaluate the effect of hydroalcoholic and aqueous extract of Althaea officinalis on the histopathologic parameters of second- and third-degree burns in mice. Experimental approach: The mice were randomly divided into 9 groups (n=6) including control and treatment groups. Experimental second- and third-degree burns were induced on dorsal skin of the mice by a metal plate (2 cm in diameter) under general anesthesia. Control and treatment groups were treated twice daily for 21 days topically. Normal saline was used for control group and hydroalcoholic and aqueous extracts of Althaea officinalis in three concentrations (1%, 2% and 4%) were used for the treatment groups. All of the preparations were made in hydrogel base. Finally, the histopathological parameters of the burned skin including polymorphonuclear (PMN) migration, epithelialization, angiogenesis and collagenization were scored and total score of wound healing was calculated. Key Results: The findings showed that topical application of both hydroalcoholic and aqueous extracts of Althaea officinalis attenuates the wound healing process. they reduce PMN migration, collagenization, epithelialization and angiogenesis scores compared to control group. Conclusions & Implications: Althaea officinalis can be used as an adjunct medication to attenuate wound healing in skin burns.

scholarly journals Perbandingan Pemberian Madu Lokal Bunga Cengkeh (Syzygium aromaticum) dan Madu Impor Bunga Manuka (Leptospermum scoparium) Secara Topikal Terhadap Penyembuhan Luka Sayat Pada Mencit Putih (Mus musculus)

2019 ◽  
Vol 6 (2) ◽  
pp. 25
Author(s):  
Nikita Tenritojang Mustafa ◽  
Dini Kurnia Ikliptikawati ◽  
Abdul Wahid Jamaluddin
Keyword(s):  
Wound Healing ◽  
Povidone Iodine ◽  
Healing Process ◽  
Colour Change ◽  
Negative Control Group ◽  
Negative Control ◽  
Wound Dressings ◽  
Control Group ◽  
Wound Healing Process ◽  
Syzygium Aromaticum

ABSTRAK Luka merupakan trauma fisik mengakibatkan kulit terbuka. Luka merupakan jalur masuk bagi mikroorganisme dari luar penyebab infeksi. Dahulu madu dimanfaatkan untuk mengobati luka bakar dan luka sayat. Madu memiliki keunggulan yakni memiliki hidrogen peroksida, eugenol dan methylglyoxal yang merupakan antibiotik yang unggul. Penelitian ini dilakukan dengan uji ekperimental. Hewan coba dibagi dalam 4 kelompok. Setiap kelompok diberikan perlakukan antara lain: dengan pemberian madu lokal, madu impor, dan salep povidone iodine (kontrol positif), dan kelompok kontrol negatif tanpa perlakuan. Dilanjutkan pemantauan dan pencatatan untuk proses penyembuhan tiap kelompok. Hal ini diukur dengan indikator antara lain: luas luka, kelembaban luka, warna, serta perubahan keropeng. Pada penelitian ini ditemukan bahwa dengan pemberian madu mempercepat proses penyembuhan luka dibandingkan dengan pemberian air dan povidone iodine (p<0,05). Selain itu ditemukan madu lokal memberikan efek penyembuhan luka yang lebih cepat dibandingkan madu impor. Pemberian madu memberikan efek yang lebih baik terhadap penyembuhan luka. Dibandingkan dengan pemberian salep Povidone iodine dan tanpa perlakuan.Kata kunci : Madu Bunga Cengkeh, Madu Bunga Manuka, Waktu penyebuhan, Warna Luka, Kelembaban. ABSTRACT Wound is a physical trauma causes discontinuity, Leads physiological dysfunction. Wound is an entry point for microorganisms from outside as source of infection. In the past, honey was used to treat burns and cuts. Honey is a natural ingredient as a moist wound-dressings. Besides that, honey contain hydrogen peroxide, eugenol, and methylglyoxal which is a superior antibiotic. This research was carried out by experimental test. The experimental animals were divided into 4 groups, each group consisted of 6 animals. Each group is treated, among others; by giving local honey, imported honey, povidone iodine (positive control), and negative control group without a treatment. Continued monitoring and recording for the healing process of each group. Statistical analysis is performed by calculation of frequency distribution and Independent-t, Anova, and regression analysis to determine the relationship between each variable. Statistical test results are significant if the value of p <0.05. This research found that addition of honeyaccelerated the wound healing process compared to the administration of water and povidone iodine (p <0.05). This is measured by indicators including; wide wounds, moisture wounds, colour change, and scab changes. By giving honey the perfect wound healing process begins on the 11th day until the 13th day. Besides that, local honey is found to provide healing effects that are faster than imported honey. Giving honey provides a better effect on wound healing. compared with Povidone iodine and without a treatment Keywords: Clove Flower Honey, Manuka Flower Honey, Time of Wasting, Colour of Wound, Moisture

scholarly journals Extract of haruan (Channa striata) extract increasing reepithelialisation count in wound healing process on wistar rat’s buccal mucosa

2016 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Devintha Ayu Mellyana Tamales ◽  
Nurdiana Dewi ◽  
Lena Rosida
Keyword(s):  
Wound Healing ◽  
Buccal Mucosa ◽  
Healing Process ◽  
Negative Control ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Epithelial Thickness ◽  
Significant Difference ◽  
Post Hoc

<p>Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The aim of this study was to examine the effect of haruan extract on reepithelialization of wistar rats’ buccal mucosa wound healing on day 3, 5, 7, and 10 histopathologically. This study was true experimental with posttest with control design. Samples were divided in 16 treatment groups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, each treatment was performed for 10 days. Epithelial thickness count reached its peak on day 10. Mean epithelial thickness scoring of each group was 50.40 µm; 56.85 µm; 62.81 µm; 38.28 µm respectively. Two way Anova and Post Hoc LSD tests presented there was a significant difference between negative control and haruan extract groups. Haruan extract treatment significantly increases epithelial thickness count in wound healing process.</p>

scholarly journals Ellagic Acid Activity in Healing Process of Incision Wound on Male Albino Rats (Rattus norvegicus)

2017 ◽  
Vol 3 (6) ◽  
pp. 224
Author(s):  
Hardany Primarizky ◽  
Wiwik Misaco Yuniarti ◽  
Bambang Sektiari Lukiswanto
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Ellagic Acid ◽  
Healing Process ◽  
Control Group ◽  
Albino Rats ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Significant Difference ◽  
Incision Wound

This study was conducted to prove whether ellagic acid can be used as a regulator of incision wound healing process in male albino rats as experimental animals. A total of 24 male healthy rats based on physical examination, aged at 3 months old and weight at 150-180 grams used in this study. Rats were divided into three groups and each group has 8 replications, group of control (P0) has no incision wound and no medication, treatment group (P1) has incision wound and treated with Betadine® and (P2) has incision wound and treated with 2,5 % ellagic acid. The variable that observed are histopathology changes (collagen, PMN, angiogenesis and fibrosis) from the male albino rats. The samples collection was taken at day 15 and data were collected and analyzed by Analysis of Variance (ANOVA) test. The result of collagen and PMN cells score in the control group (P0) were 1.80 ± 0.16 and 1.6 ± 0.08 with mild angiogenesis and no fibrosis. The results in the treatment (P2) group, where the collagen and PMN scores were 1.60 ± 0.08 and 1.5 ± 0.8, accompanied by mild angiogenesis and fibrosis. Meanwhile, in the treatment (P1) group, the results of scoring of collagen and PMN were lower than the other groups, 0, 80 ± 0.08 and 0.6 ± 0.16 accompanied by moderate angiogenesis and fibrosis. There was a significant difference of collagen scoring, PMN cells number, fibrosis and angiogenesis among the treatment groups (p<0.05). P1 treatment group has significant different with treatment groups P0 and P2 (p<0.05), but there is no significant difference between treatment groups P0 and P2 (p>0.05).  From the results, it can be concluded that the topical application of ellagic acid ointment was proved to provide healing process on incision wound. Topical administration of ellagic acid ointment for 14 days was able to improve the skin condition and promoted the wound healing.Keywords: Ellagic acid ; wound healing ; collagen ; angiogenesis ; fibrosis ; anti-inflammatory ; antioxidant ; antibacterial.

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