scholarly journals HE EFFECT OF BINJAI LEAVES EXTRACT (Mangifera caesia) ON INCISIONAL BACK WOUND OF MICE (Mus musculus) TO THE NUMBER OF NEUTROPHIL CELLS (Preliminary Study of Drug Preparations for Wound Healing)

2020 ◽  
Vol 5 (1) ◽  
pp. 21
Author(s):  
Desty Ayu Dwiyanti ◽  
Irham Taufiqurrahman ◽  
Didit Aspriyanto
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Group Design ◽  
Inflammatory Phase ◽  
Significant Difference

Background: Ethanol extract of binjai leaves contain bioactive compounds in the form of flavonoid and saponin which is potential as anti-inflammatory, so it will be able to control the wound healing process. Binjai leaves extract can affect the number of neutrophil on inflammatory phase, as it is characterized by neutrophil cells infiltration to the wound tissue, so its number will increase at the beginning of inflammatory phase and decrease on the 3rd day. Objective: To prove the effect of binjai leaves extract in 1 mg/mL, 2 mg/mL, and 3 mg/mL which given topically to the number of neutrophil in day 1, 3, and 5 of wound incision on the back of male mice. Methods: The study design was a pure laboratory experimental study with post-test only with control group design which involved 36 mice divided into 4 groups: negative control group design which was given BR2 feed, treatment group which was given binjai leaves extract in 1 mg/mL, 2 mg/mL, and 3 mg/mL. Results: The result of One-way ANOVA showed that there was significant difference of all groups in day 1 (p=0.000), day 3 (p=0.001), and day 5 (p=0.000). Post-Hoc LSD test showed that there was significant difference (p<0.05) between the negative control group and treatment groups. Conclusion: Binjai leaves extract has an effect to increase the number of neutrophil in day 1 and decrease the number of neutrophil in day 3 and 5 on the healing of back wound incision in mice.

scholarly journals Effect of haruan (Channa striata) extract on fibroblast cells count in wound healing

2016 ◽  
Vol 1 (2) ◽  
pp. 226
Author(s):  
Ardi Siswanto ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Fibroblast Cells ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The purpose of this study is to assess the effect of haruan extract on wistar rat’s buccal mucosa wound healing on day 3, 5, and 7 histopathologically. This study was experimental with post-test with control group design. Samples were consisted of 4 treatment groups, 25%, 50%, 100% haruan extract treatment groups and negative control treated with<em> aquadest</em> for 7 days. Mean fibroblast cells count reached its peak on day 7. Mean fibroblast cells count in each group was 26.995; 40.500; 49.165; 24.495 respectively. Two way ANOVA and Post Hoc Least Significant Difference tests indicated there was a significant difference (p&lt;0.05) between haruan extract treatment group and negative control. In conclusion, haruan extract treatment significantly increase fibroblast cells count in wound healing process.

scholarly journals Effect of haruan (channa striata) extract on fibroblast cells count in wound healing

2016 ◽  
Vol 1 (2) ◽  
pp. 89
Author(s):  
Ardi Siswanto ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Fibroblast Cells ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The purpose of this study is to assess the effect of haruan extract on wistar rat’s buccal mucosa wound healing on day 3, 5, and 7 histopathologically. This study was experimental with post-test with control group design. Samples were consisted of 4 treatment groups, 25%, 50%, 100% haruan extract treatment groups and negative control treated with aquadest for 7 days. Mean fibroblast cells count reached its peak on day 7. Mean fibroblast cells count in each group was 26.995; 40.500; 49.165; 24.495 respectively. Two way ANOVA and Post Hoc Least Significant Difference tests indicated there was a significant difference (p<0.05) between haruan extract treatment group and negative control. In conclusion, haruan extract treatment significantly increase fibroblast cells count in wound healing process.

scholarly journals Pengaruh gel ekstrak daun binahong (Anredera cordifolia) 5% terhadap peningkatan osteoblas pada proses penyembuhan luka pasca pencabutan gigi tikus strain WistarEffect of 5% binahong (Anredera cordifolia) leaf extract in increasing the osteoblast amount at the tooth extraction wound healing process of Wistar rat

2020 ◽  
Vol 32 (1) ◽  
pp. 9
Author(s):  
Jannatus Salis Sa'diyah ◽  
Dewi Ayu Septiana ◽  
Nanda Nailul Farih ◽  
Juwita Raditya Ningsih
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design

Pendahuluan: Pencabutan gigi di dunia kedokteran gigi adalah tindakan yang sering dilakukan. Pasca terjadinya pencabutan gigi, maka tulang pada soket akan rusak. Penyembuhan tulang ditandai dengan terbentuknya sel-sel osteoblas baru. Daun binahong merupakan daun yang mengandung flavonoid, alkaloid, terpenoid, asam askorbat, saponin, tanin dan asam oleanolik. Kandungan dari daun binahong dapat meningkatkan jumlah osteoblas pasca pencabutan gigi. Tujuan dari penelitian adalah menganalisis pengaruh pemberian gel ekstrak daun binahong (Anredera cordifolia) konsentrasi 5% terhadap peningkatan osteoblas pada proses penyembuhan luka pasca pencabutan gigi. Metode: Jenis penelitian ini merupakan penelitian eksperimental laboratorium murni dengan rancangan post test only control group design. Subyek penelitian berupa 45 ekor tikus dibagi menjadi 3 kelompok, yaitu kelompok gel ekstrak daun binahong, kontrol negatif dan kontrol positif. Gigi insisivus sentral kiri rahang bawah dilakukan pencabutan gigi kemudian diaplikasikan gel ekstrak daun binahong 5%, CMC-Na 1% dan iod gliserin. Tikus diterminasi pada hari ke 3,5,7,14 dan 21 pasca dilakukan pencabutan gigi untuk selanjutnya dibuat preparat histologis dengan pengecatan hematoksilin eosin. Jumlah sel osteoblas dihitung dengan optilab dan software image raster. Hasil: Hasil uji parametrik one way anova menunjukan terdapat perbedaan jumlah osteoblas yang bermakna antar kelompok (p<0,05) pada masing-masing hari. Hasil LSD (Least Significance Different) menunjukkan terdapat perbedaan yang bermakna (p<0,05) pada hari ke 5,7, 14 dan 21 antara kelompok perlakuan dengan kontrol negatif dan terdapat perbedaan yang bermakna pada hari ke 7 dan 14 antara kelompok perlakuan dan kontrol positif. Simpulan: Gel ekstrak daun binahong konsentrasi 5% berpengaruh dalam meningkatkan jumlah sel osteoblas pasca dilakukan pencabutan gigi.Kata kunci: Pencabutan gigi, gel ekstrak daun binahong, osteoblas. ABSTRACTIntroduction: Tooth extraction is the most common treatment in dentistry. After tooth extraction, the bone in the socket will be damaged. The formation of new osteoblast characterises bone healing. Binahong leaves contain flavonoids, alkaloids, terpenoids, ascorbic acid, saponins, tannins and oleanolic acids. This research was aimed to investigate the effectivity of binahong (Anredera cordifolia) leaf extract gel with a concentration of 5% in increasing the number of osteoblasts at the tooth extraction wound healing process. Methods: This research was an experimental laboratory with a post-test only control group design. Forty-five rats were divided into 3 groups; binahong leaf gel group, negative control group, and positive control group. Mandibular left central incisive was extracted, then binahong leaf gel, 1% CMC-Na, and glycerin were applied on the wound socket in different groups. All rats then terminated at day 3, 5, 7, 14, and 21 post-extraction and processed for the histological examination then coloured by haematoxylin-eosin. The number of osteoblasts was calculated by Optilab and raster image software. Results: The ANOVA test result showed significant differences in the number of osteoblasts among groups (p<0.05) on each day. The LSD (Least Significance Different) test result showed significant differences (p<0.05) at day 5, 7, 14, and 21 post-extraction, between binahong leaf gel group compared to the negative control group, and also at day 7 and 14 post-extraction between binahong leaf gel group compared to the positive control group. Conclusion: Binahong leaf extract gel with a concentration of 5% is effective in increasing the number of osteoblast in the wound healing process after tooth extraction.Keywords: Tooth extraction, Binahong leaf extract gel, osteoblast.

scholarly journals Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Muhammad Reza Setiawan ◽  
Nurdiana Dewi ◽  
Ika Kustiyah Oktaviyanti
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Channa Striata ◽  
Post Hoc

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract hassubstantial properties such as albumin, Zn,Cu, and Feto accelerate wound healing. The purpose of this study is to assessthe effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. Thisstudy was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatmentgroups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, eachtreatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of eachgroup was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant differencebetween negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increasedneocapillaries count in wound healing process.

scholarly journals Perbandingan Pemberian Madu Lokal Bunga Cengkeh (Syzygium aromaticum) dan Madu Impor Bunga Manuka (Leptospermum scoparium) Secara Topikal Terhadap Penyembuhan Luka Sayat Pada Mencit Putih (Mus musculus)

2019 ◽  
Vol 6 (2) ◽  
pp. 25
Author(s):  
Nikita Tenritojang Mustafa ◽  
Dini Kurnia Ikliptikawati ◽  
Abdul Wahid Jamaluddin
Keyword(s):  
Wound Healing ◽  
Povidone Iodine ◽  
Healing Process ◽  
Colour Change ◽  
Negative Control Group ◽  
Negative Control ◽  
Wound Dressings ◽  
Control Group ◽  
Wound Healing Process ◽  
Syzygium Aromaticum

ABSTRAK Luka merupakan trauma fisik mengakibatkan kulit terbuka. Luka merupakan jalur masuk bagi mikroorganisme dari luar penyebab infeksi. Dahulu madu dimanfaatkan untuk mengobati luka bakar dan luka sayat. Madu memiliki keunggulan yakni memiliki hidrogen peroksida, eugenol dan methylglyoxal yang merupakan antibiotik yang unggul. Penelitian ini dilakukan dengan uji ekperimental. Hewan coba dibagi dalam 4 kelompok. Setiap kelompok diberikan perlakukan antara lain: dengan pemberian madu lokal, madu impor, dan salep povidone iodine (kontrol positif), dan kelompok kontrol negatif tanpa perlakuan. Dilanjutkan pemantauan dan pencatatan untuk proses penyembuhan tiap kelompok. Hal ini diukur dengan indikator antara lain: luas luka, kelembaban luka, warna, serta perubahan keropeng. Pada penelitian ini ditemukan bahwa dengan pemberian madu mempercepat proses penyembuhan luka dibandingkan dengan pemberian air dan povidone iodine (p<0,05). Selain itu ditemukan madu lokal memberikan efek penyembuhan luka yang lebih cepat dibandingkan madu impor. Pemberian madu memberikan efek yang lebih baik terhadap penyembuhan luka. Dibandingkan dengan pemberian salep Povidone iodine dan tanpa perlakuan.Kata kunci : Madu Bunga Cengkeh, Madu Bunga Manuka, Waktu penyebuhan, Warna Luka, Kelembaban. ABSTRACT Wound is a physical trauma causes discontinuity, Leads physiological dysfunction. Wound is an entry point for microorganisms from outside as source of infection. In the past, honey was used to treat burns and cuts. Honey is a natural ingredient as a moist wound-dressings. Besides that, honey contain hydrogen peroxide, eugenol, and methylglyoxal which is a superior antibiotic. This research was carried out by experimental test. The experimental animals were divided into 4 groups, each group consisted of 6 animals. Each group is treated, among others; by giving local honey, imported honey, povidone iodine (positive control), and negative control group without a treatment. Continued monitoring and recording for the healing process of each group. Statistical analysis is performed by calculation of frequency distribution and Independent-t, Anova, and regression analysis to determine the relationship between each variable. Statistical test results are significant if the value of p <0.05. This research found that addition of honeyaccelerated the wound healing process compared to the administration of water and povidone iodine (p <0.05). This is measured by indicators including; wide wounds, moisture wounds, colour change, and scab changes. By giving honey the perfect wound healing process begins on the 11th day until the 13th day. Besides that, local honey is found to provide healing effects that are faster than imported honey. Giving honey provides a better effect on wound healing. compared with Povidone iodine and without a treatment Keywords: Clove Flower Honey, Manuka Flower Honey, Time of Wasting, Colour of Wound, Moisture

scholarly journals Ekstrakharuan(Channa striata)secara efektif menurunkan jumlah limfosit fase inflamasi dalam penyembuhan luka (Extract of haruan (Channa striata) decreases lymphocyte count in inflammatory phase of wound healing process effectively)

2014 ◽  
Vol 13 (3) ◽  
pp. 176
Author(s):  
Arisa Izzaty ◽  
Nurdiana Dewi ◽  
Dewi Indah Noviana Pratiwi
Keyword(s):  
Wound Healing ◽  
Lymphocyte Count ◽  
Healing Process ◽  
Simple Random Sampling ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Inflammatory Phase ◽  
Channa Striata

Haruan is river-water fish usually consumed by South Kalimantan people. Haruan extract contains albumin, Zn, Fe,Cu and unsaturated fatty acid which can act as antiinflammatory substances and accelarate wound healing. The aim ofthis study is to examine effectiveness of haruan extract on lymphocyte count in infammatory phase of wound healingprocess and compare it to ibuprofen. This study used true experimental method with post-test only control group designand simple random sampling. Samples were divided into 5 treatment groups:which were given the extract for 7 sevendays: 25% haruan extract group, 50% haruan extract group, 100% haruan extract group, negative control K(-) usingaquadest, positive control K(+) given ibuprofen suspension with dosage of 20mg/kg weight/day. Lymphocyte cells wereobserved using microscope and counted per field of view. Lymphocyte count reached its peak on day 5. Lymphocytecount means in each group were 4,2 ± 0,566; 1± 0,000; 3,8 ± 2,546; 7,1 ± 0,141; dan 7,5 ± 3,818 respectively. Two way ANOVA and post hoc LSD tests showed that lymphocyte count significantly decreased in 50% and 100% treatmentgroups (p<0,05) and has equal effect with ibuprofen in decreasing lymphocyte count. This study concluded that haruanextract significantly decreased lymphocyte count in wound healing process.

scholarly journals Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Muhammad Reza Setiawan ◽  
Nurdiana Dewi ◽  
Ika Kustiyah Oktaviyanti
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference ◽  
Channa Striata ◽  
Post Hoc

<p>Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties suchas albumin, Zn,Cu, andFeto accelerate wound healing. The purposeof this study is to assess the effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. This study was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatment groups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, each treatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of each group was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant difference between negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increased neocapillaries count in wound healing process.</p><p>Haruan adalah ikankhas Kalimantan yangberpotensi mempercepat penyembuhanluka. Ekstrak ikan haruan mengandung zat penting seperti albumin, Zn , Cu, dan Fe untuk mempercepat penyembuhan luka. Penelitian ini dimaksudkan untuk mengetahui pengaruh ekstrak ikan haruan pada proses penyembuhan luka pada mukosa bukal tikus wistar hari ke-1, 3, 5, dan 7 secara histopatologi. Penelitian eksperimental murni dengan pretest-posttest with control group ini, meliputi 4 kelompok,yaitu kelompok hewan yang diberi ekstrak haruan selama7 hari dengan konsentrasi berturut-turut 25%, 50%, 100%, dan kelompok kontrol negatif diberi akuades. Hasilnya menunjukkan bahwa jumlah neokapiler mencapai puncak pada hari ke-5. Rerata jumlah neokapiler pada tiap kelompok berturut adalah 12; 16,5; 18,5; dan 6. Uji Anova 2-jalur dan uji post hoc LSDmenunjukkan adanya perbedaan bermakna antara kontrol negatif dan ekstrak ikan haruan. Berdasarkan hasil penelitian, disimpulkan bahwa pemberian ekstrak ikan haruan secara bermakna meningkatkan jumlah neokapiler pada proses penyembuhan luka.</p>

scholarly journals Effectiveness Cream and Ointment of Spirulina platensis extract against amount of Fibroblas and Wound Area: Study on white rats whose skin is incised

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Bimby Irenesia ◽  
Renni Yuniarti ◽  
Endang Mahati
Keyword(s):  
Wound Healing ◽  
Spirulina Platensis ◽  
Wistar Rats ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Wound Area ◽  
White Rats

Spirulina platensishas been well-known as an abundant protein and phycocyanin. Spirulina platensis has the effect of anti-inflammatory, so it is widely used to apply cosmetics and biomedicine in the healing process of wounds. To prove that the administration of an extract ofSpirulina platensiscan increase the number of fibroblasts and accelerate the wound healing process in Wistar rats with an incision on their skin. This study used a randomizedpost-test only control group design. The skins of thirty (30) male Wistar rats were incised. This treatment was divided into 4 groups, namely the group that received the 0,1% cream (X1) of S.platensisextract, and the group that was administered with the 0,1% ointment (X2) of S.platensis extract; the negative control group was only treated by a saline solution (C1), and the positive control group was with the administration of Gentamycin 0,1% ointment (C2). The total numbers of fibroblasts were examined on day 14 and calculated using the method of tissue biopsy and hematoxylin-eosin (HE) staining. The wound was checked after 14-day Data analysis was performed with a one-wayANOVA test and was continued by Post Hoc Test LSD. The total numbers of fibroblasts on day 14 from the group of XI, X2, C1, and C2 were 15.00; 17,50; 3,83, 10,33 with p<0.001. The average size of the wounds on day 14 from the groups of X1, X2, C1, and C2 was 551,78; 435,76; 1795,95; 1193,57 with p<0.001, respectively. PostHoc Testresults on the total number of fibroblasts and extensive wound area showed significant differences between them. Administration of the 0.1% ointment of Spirulina platensisextract believed to potentially increasing the number of fibroblasts and accelerate the process of wound healing.

scholarly journals Sappanwood (Caesalpinia sappan) Extract Gel Do Not Heal Skin Excisional Wound on Balb/c Mice

2019 ◽  
Vol 12 (1) ◽  
pp. 375-381
Author(s):  
Andri Rezano ◽  
Thifal Indra Zhalfani Siregar ◽  
Adi Santosa Maliki ◽  
Melia Juwita Adha ◽  
Listya Hanum
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Healing Process ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Caesalpinia Sappan ◽  
Significant Difference ◽  
Group Control ◽  
Excisional Wound

Caesalpinia sappan has been studied for its biological activities in the wound healing process. Therefore, this study is conducted to investigate the wound healing activity of sappanwood extract in an excisional wound of Balb/c mice. Animal experiments were designated with, positive control group, negative control group, and three groups of treatment (G1, G2 and G3). The groups consist of povidone iodine ointment, blank gel, 5%, 30%, 90% concentration of sappanwood extract gel, respectively. All groups were applied topically on the surface of the biopsy wound once daily for 10 days. The wounds of the 2nd, 8th and 10th day of treatment were observed and measured. There was a significant difference from each group on day 2, 6 and 10 after wound induction (p=0,001, p=0,001 and p=0,006). The tendency of wound closure happened in positive group control, negative group control and G1. Negative control group showed the highest percentage of wound closure amongst all groups. Meanwhile, in G2 and G3 showed less percentage of closure and healing compared to the others. Despite there is a tendency of ethanolic C. sappan extract gels to the wound healing, administration of C. sappan extract gels topically do not heal the skin excisional wound.

scholarly journals BIOSYNTHESIS OF pH RESPONSIVE SHAPE MEMORY HYDROGEL AND ITS BIOMEDICAL POTENTIAL

2017 ◽  
Vol 6 (3) ◽  
pp. 946-952
Author(s):  
AHMAD MOHAMMED GUMEL
Keyword(s):  
Wound Healing ◽  
Shape Memory ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Ph Responsive ◽  
Tissue Samples ◽  
Significant Difference ◽  
Biomedical Potential

Multifunctional hydrogels combining the capabilities of cellular pH responsiveness and shape memory, are highly promising for the realization of smart membrane filters, controlled drug released devices, and functional tissue-engineering scaffolds. Free radical biocatalytic polymerization catalyzed by immobilized Candida antarctica lipase B was used to fabricate the pH-responsive and shape memory hydrogel using medium-chain-length poly-3-hydroxyalkanoates-co-polyethylene glycol methacrylate (PHA-PEGMA) as macromer. The accelerative wound healing potential of the biosynthesized smart PHA based hydrogel was evaluated herein. The thermal stability of the macromere highly depends on the PEGMA fraction from 10 to 50% (mass). Similarly, the change in PEGMA fraction was also found to highly influence the hydrogel’s hydration rate (r) from 2.83 x 10-5 to 7.63  x 10-5  mL/s.  The hydrogel’s equilibrium weight swelling ratio (qe), protein release and its diffusion coefficient (Dm) were all found to be pH dependent. For example, increasing the phosphate buffer pH from 2.4 to 13 resulted in increased qe from 2 to 16 corresponding to the enlarging of network pore size (ξ) from 150 to 586 nm.The biomedical potential of the synthesized material based on its accelerative wound healing in rats was observed. Biochemical, histomorphometric and immunohistomorphometric analyses revealed a significant difference in area closure and re-epithelialization on days 7 and 14 in PPH or Intrasite® gel group compared to gum acacia or PEGMA-alone groups. Furthermore, wounds dressed with PPH or Intrasite® gel showed evident collagen deposition, enhanced fibrosis and extensively organized angiogenesis on day 14 compared to the negative control group. The findings suggested that topical application of PPH accelerated the rats’ wound healing process by improving angiogenesis attributed to the increased microvessel density (MVD) and expressions of VEGF-A in tissue samples. Thus, PPH has been demonstrated to be effective in the treatment of cutaneous wounds in rats, and could be a potential novel agent in the management and acceleration of wound healing in humans and animals.

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