scholarly journals Effect of haruan (channa striata) extract on fibroblast cells count in wound healing

2016 ◽  
Vol 1 (2) ◽  
pp. 89
Author(s):  
Ardi Siswanto ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Fibroblast Cells ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The purpose of this study is to assess the effect of haruan extract on wistar rat’s buccal mucosa wound healing on day 3, 5, and 7 histopathologically. This study was experimental with post-test with control group design. Samples were consisted of 4 treatment groups, 25%, 50%, 100% haruan extract treatment groups and negative control treated with aquadest for 7 days. Mean fibroblast cells count reached its peak on day 7. Mean fibroblast cells count in each group was 26.995; 40.500; 49.165; 24.495 respectively. Two way ANOVA and Post Hoc Least Significant Difference tests indicated there was a significant difference (p<0.05) between haruan extract treatment group and negative control. In conclusion, haruan extract treatment significantly increase fibroblast cells count in wound healing process.

scholarly journals Effect of haruan (Channa striata) extract on fibroblast cells count in wound healing

2016 ◽  
Vol 1 (2) ◽  
pp. 226
Author(s):  
Ardi Siswanto ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Fibroblast Cells ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The purpose of this study is to assess the effect of haruan extract on wistar rat’s buccal mucosa wound healing on day 3, 5, and 7 histopathologically. This study was experimental with post-test with control group design. Samples were consisted of 4 treatment groups, 25%, 50%, 100% haruan extract treatment groups and negative control treated with<em> aquadest</em> for 7 days. Mean fibroblast cells count reached its peak on day 7. Mean fibroblast cells count in each group was 26.995; 40.500; 49.165; 24.495 respectively. Two way ANOVA and Post Hoc Least Significant Difference tests indicated there was a significant difference (p&lt;0.05) between haruan extract treatment group and negative control. In conclusion, haruan extract treatment significantly increase fibroblast cells count in wound healing process.

scholarly journals Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Muhammad Reza Setiawan ◽  
Nurdiana Dewi ◽  
Ika Kustiyah Oktaviyanti
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Channa Striata ◽  
Post Hoc

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract hassubstantial properties such as albumin, Zn,Cu, and Feto accelerate wound healing. The purpose of this study is to assessthe effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. Thisstudy was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatmentgroups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, eachtreatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of eachgroup was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant differencebetween negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increasedneocapillaries count in wound healing process.

scholarly journals Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Muhammad Reza Setiawan ◽  
Nurdiana Dewi ◽  
Ika Kustiyah Oktaviyanti
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference ◽  
Channa Striata ◽  
Post Hoc

<p>Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties suchas albumin, Zn,Cu, andFeto accelerate wound healing. The purposeof this study is to assess the effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. This study was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatment groups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, each treatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of each group was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant difference between negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increased neocapillaries count in wound healing process.</p><p>Haruan adalah ikankhas Kalimantan yangberpotensi mempercepat penyembuhanluka. Ekstrak ikan haruan mengandung zat penting seperti albumin, Zn , Cu, dan Fe untuk mempercepat penyembuhan luka. Penelitian ini dimaksudkan untuk mengetahui pengaruh ekstrak ikan haruan pada proses penyembuhan luka pada mukosa bukal tikus wistar hari ke-1, 3, 5, dan 7 secara histopatologi. Penelitian eksperimental murni dengan pretest-posttest with control group ini, meliputi 4 kelompok,yaitu kelompok hewan yang diberi ekstrak haruan selama7 hari dengan konsentrasi berturut-turut 25%, 50%, 100%, dan kelompok kontrol negatif diberi akuades. Hasilnya menunjukkan bahwa jumlah neokapiler mencapai puncak pada hari ke-5. Rerata jumlah neokapiler pada tiap kelompok berturut adalah 12; 16,5; 18,5; dan 6. Uji Anova 2-jalur dan uji post hoc LSDmenunjukkan adanya perbedaan bermakna antara kontrol negatif dan ekstrak ikan haruan. Berdasarkan hasil penelitian, disimpulkan bahwa pemberian ekstrak ikan haruan secara bermakna meningkatkan jumlah neokapiler pada proses penyembuhan luka.</p>

scholarly journals HE EFFECT OF BINJAI LEAVES EXTRACT (Mangifera caesia) ON INCISIONAL BACK WOUND OF MICE (Mus musculus) TO THE NUMBER OF NEUTROPHIL CELLS (Preliminary Study of Drug Preparations for Wound Healing)

2020 ◽  
Vol 5 (1) ◽  
pp. 21
Author(s):  
Desty Ayu Dwiyanti ◽  
Irham Taufiqurrahman ◽  
Didit Aspriyanto
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Group Design ◽  
Inflammatory Phase ◽  
Significant Difference

Background: Ethanol extract of binjai leaves contain bioactive compounds in the form of flavonoid and saponin which is potential as anti-inflammatory, so it will be able to control the wound healing process. Binjai leaves extract can affect the number of neutrophil on inflammatory phase, as it is characterized by neutrophil cells infiltration to the wound tissue, so its number will increase at the beginning of inflammatory phase and decrease on the 3rd day. Objective: To prove the effect of binjai leaves extract in 1 mg/mL, 2 mg/mL, and 3 mg/mL which given topically to the number of neutrophil in day 1, 3, and 5 of wound incision on the back of male mice. Methods: The study design was a pure laboratory experimental study with post-test only with control group design which involved 36 mice divided into 4 groups: negative control group design which was given BR2 feed, treatment group which was given binjai leaves extract in 1 mg/mL, 2 mg/mL, and 3 mg/mL. Results: The result of One-way ANOVA showed that there was significant difference of all groups in day 1 (p=0.000), day 3 (p=0.001), and day 5 (p=0.000). Post-Hoc LSD test showed that there was significant difference (p<0.05) between the negative control group and treatment groups. Conclusion: Binjai leaves extract has an effect to increase the number of neutrophil in day 1 and decrease the number of neutrophil in day 3 and 5 on the healing of back wound incision in mice.

scholarly journals Extract of haruan (Channa striata) extract increasing reepithelialisation count in wound healing process on wistar rat’s buccal mucosa

2016 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Devintha Ayu Mellyana Tamales ◽  
Nurdiana Dewi ◽  
Lena Rosida
Keyword(s):  
Wound Healing ◽  
Buccal Mucosa ◽  
Healing Process ◽  
Negative Control ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Epithelial Thickness ◽  
Significant Difference ◽  
Post Hoc

<p>Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The aim of this study was to examine the effect of haruan extract on reepithelialization of wistar rats’ buccal mucosa wound healing on day 3, 5, 7, and 10 histopathologically. This study was true experimental with posttest with control design. Samples were divided in 16 treatment groups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, each treatment was performed for 10 days. Epithelial thickness count reached its peak on day 10. Mean epithelial thickness scoring of each group was 50.40 µm; 56.85 µm; 62.81 µm; 38.28 µm respectively. Two way Anova and Post Hoc LSD tests presented there was a significant difference between negative control and haruan extract groups. Haruan extract treatment significantly increases epithelial thickness count in wound healing process.</p>

scholarly journals Extract of haruan (channa striata) extract increasing reepithelialisation count in wound healing process on wistar rat’s buccal mucosa

2016 ◽  
Vol 1 (1) ◽  
pp. 12
Author(s):  
Devintha AM. Tamales ◽  
Nurdiana Dewi ◽  
Lena Rosida
Keyword(s):  
Wound Healing ◽  
Buccal Mucosa ◽  
Healing Process ◽  
Negative Control ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Epithelial Thickness ◽  
Significant Difference ◽  
Post Hoc

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties such as albumin, Zn, Cu, and Fe to accelerate wound healing. The aim of this study was to examine the effect of haruan extract on reepithelialization of wistar rats’ buccal mucosa wound healing on day 3, 5, 7, and 10 histopathologically. This study was true experimental with posttest with control design. Samples were divided in 16 treatment groups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, each treatment was performed for 10 days. Epithelial thickness count reached its peak on day 10. Mean epithelial thickness scoring of each group was 50.40 µm; 56.85 µm; 62.81 µm; 38.28 µm respectively. Two way Anova and Post Hoc LSD tests presented there was a significant difference between negative control and haruan extract groups. Haruan extract treatment significantly increases epithelial thickness count in wound healing process.

scholarly journals Investigating the healing effect of the hydroalcoholic extract of pomegranate seed (Punica granatum) on the full thickness wound in rabbit

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Ali Asghar Hemmati ◽  
Iran Rashidi ◽  
Sedigheh Dahanzadeh ◽  
Mahmoud Moeini
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Punica Granatum ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Hydroxyproline Content ◽  
Tissue Samples ◽  
Treatment Groups ◽  
Wound Recovery

Objective: This research designed to investigate the wound healing process with pomegranate hydroalcoholic seed extract (Punica granatum) in comparison with no-treatment, betamethasone, phenytoin and eucerin in rabbits. Methods: The positive group including groups that received phenytoin cream (1%) and topical eucerin, respectively, twice a day to complete wound healing. negative Control group did not obtain any treatment. Treatment groups were received cream of PSE (2, 5,7,10 w/w) in eucerin and 75% w/w as purified extract twice daily. In order to measure the percentage of wound healing, the zone of the wound was evaluated daily. Histological studies were done on the 7th and 15th days of treatment. Next, hydroxyproline content of wounds healed and tensile strength of wound tissue samples were measured. Results: The results demonstrated between PSE treatment groups and eucerin animals were statistically significant aadifferences (P<0.05) in most of the days reviewed. Treatment of Rabbits with 10% PSE had the best results (complete wound recovery in 12 days). Also, this treatment showed higher hydroxyproline content and higher tissue strength. Conclusion: This research reveals that the extract of 10% PSE administrated topically has the proper potential to induce wound recovery in the wound model of rabbits. In addition, 10% PSE accelerates the healing of the wound. Further study needs to clarify the results of this research.

The Effect of Chicken (Gallus gallus domesticus) Eggshell’s Powder towards Wound Healing of Incision Wound on Male Swiss-Webster Mice

2016 ◽  
Vol 1 (4) ◽  
Author(s):  
Rahman A. Nugraha ◽  
Harijadi Pramono ◽  
Roro Wahyudianingsih
Keyword(s):  
Wound Healing ◽  
Microscopic Examination ◽  
Average Length ◽  
Healing Process ◽  
Domestic Chicken ◽  
Control Group ◽  
Wound Healing Process ◽  
Accelerate Wound Healing ◽  
Significant Difference ◽  
Incision Wound

Domestic chicken eggshells contain calcium and protein that could be useful in wound healing process. This research was conducted to determine whether domestic chicken eggshells can accelerate wound healing. Twenty one male Swiss-Webster mice  with 2 cm incision were divided into 3 groups. Negative control group were given Carboxymethyl cellulose 1% (CMC), positive control group were given feracrylum 1% (FER), and the eggshells group given domestic chicken eggshells powder (KT). Treatment and data mesurement was measured every day until  the 7th day. On the 7th  day the average length of the wound were analyzed and the skin tissue is taken for microscopic examination. Data analyzed using one-way ANOVA followed by Post Hoc Least Significant Difference (LSD) with p=0,05. On macroscopic examination, there were  highly significant differences between KT compared to CMC and highly significant differences between KT compared to FER. the average length of wound on the 7th day on KT 0,63 cm shorter than CMC 1,07 (p=0,000) and FER 0,81 cm (p=0,003). On microscopic examination, the degree of reepithelization on KT groups are severe, moderate for collagen density, fibroblasts density, subepithelial neutrophil density, degree of edema, and mild for angiogenesis. We conclude domestic chicken eggshells powder can accelerate wound healing. Key words: domestic chicken eggshells, incision wound, wound healing

scholarly journals Ellagic Acid Activity in Healing Process of Incision Wound on Male Albino Rats (Rattus norvegicus)

2017 ◽  
Vol 3 (6) ◽  
pp. 224
Author(s):  
Hardany Primarizky ◽  
Wiwik Misaco Yuniarti ◽  
Bambang Sektiari Lukiswanto
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Ellagic Acid ◽  
Healing Process ◽  
Control Group ◽  
Albino Rats ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Significant Difference ◽  
Incision Wound

This study was conducted to prove whether ellagic acid can be used as a regulator of incision wound healing process in male albino rats as experimental animals. A total of 24 male healthy rats based on physical examination, aged at 3 months old and weight at 150-180 grams used in this study. Rats were divided into three groups and each group has 8 replications, group of control (P0) has no incision wound and no medication, treatment group (P1) has incision wound and treated with Betadine® and (P2) has incision wound and treated with 2,5 % ellagic acid. The variable that observed are histopathology changes (collagen, PMN, angiogenesis and fibrosis) from the male albino rats. The samples collection was taken at day 15 and data were collected and analyzed by Analysis of Variance (ANOVA) test. The result of collagen and PMN cells score in the control group (P0) were 1.80 ± 0.16 and 1.6 ± 0.08 with mild angiogenesis and no fibrosis. The results in the treatment (P2) group, where the collagen and PMN scores were 1.60 ± 0.08 and 1.5 ± 0.8, accompanied by mild angiogenesis and fibrosis. Meanwhile, in the treatment (P1) group, the results of scoring of collagen and PMN were lower than the other groups, 0, 80 ± 0.08 and 0.6 ± 0.16 accompanied by moderate angiogenesis and fibrosis. There was a significant difference of collagen scoring, PMN cells number, fibrosis and angiogenesis among the treatment groups (p<0.05). P1 treatment group has significant different with treatment groups P0 and P2 (p<0.05), but there is no significant difference between treatment groups P0 and P2 (p>0.05).  From the results, it can be concluded that the topical application of ellagic acid ointment was proved to provide healing process on incision wound. Topical administration of ellagic acid ointment for 14 days was able to improve the skin condition and promoted the wound healing.Keywords: Ellagic acid ; wound healing ; collagen ; angiogenesis ; fibrosis ; anti-inflammatory ; antioxidant ; antibacterial.

scholarly journals FGF-2 expression and the amount of fibroblast in the incised wounds of Rattus norvegicus rats induced with Mauli banana (Musa acuminata) stem extract

2018 ◽  
Vol 50 (3) ◽  
pp. 121
Author(s):  
Didit Aspriyanto ◽  
Intan Nirwana ◽  
Hendrik Setia Budi
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Fibroblast Cells ◽  
Treatment Groups ◽  
Incised Wound ◽  
Stem Extract ◽  
Banana Stem

Background: Traditional wound treatment using herbal medicine is thought to maintain the health of families and society in general economically, effectively, and efficiently without inducing side effects. One genus of plant that can be used as a traditional medicine is the Mauli banana, indigenous to South Borneo. Mauli banana stem contains bioactive compounds, most of which are tannins along with ascorbic acid, saponin, β-carotene, flavonoids, lycopene, alkaloids, and flavonoids. Tanin has antibacterial and antioxidant effects at low concentrations, as wells as antifungal ones at high concentrations. Purpose: This study aimed to analyze the effects of Mauli banana stem extract at concentrations of 25%, 37.5%, and 50% on the quality of incised wound healing in male Rattus norvegicus rats by assessing FGF-2 expression and fibroblast concentration on days 3 and 7. Methods: This research represented an experimental laboratory-based investigation involving 32 rats of the Rattus norvegicus strain aged 2-2.5 months old. Sampling was performed using a simple random sampling technique since the research population was considered homogeneous and divided into 8 treatment groups (C3, M3-25, M3-37.5, M3-50, C7, M7-25, M7-37.5, M7-50). The rats in each group were anesthetized before their back was incised with length and width of 15x15mm with a depth of 2mm. Gel hydroxy propyl cellulose medium (HPMC) was applied to the incised wound of each rat in the control group, while stem Mauli banana extract was applied to that of each rat in the treatment groups three times a day at an interval of 6-8 hours. On day 3, four rats from each group were sacrificed, while, in the remaining groups, the same procedure was performed until day 7, at which point they (8 groups) were sacrificed for HE examination in order to assess the amount of fibroblast and for IHC examination to examine FGF-2 expression. Data regarding FGF-2 expression and the amount of fibroblast were analysed by means of One-way Anova and HSD. Results: The results showed that the Mauli banana stem extract could significantly improve the expression of FGF-2 and the amount of fibroblast cells compared to C3 and C7 groups. The highest increase in FGF-2 expression and fibroblast amount were found in all groups on days 3 and 7 treated with the Mauli banana stem extract at a concentration of 50%. Conclusion: There was an increase of FGF-2 expression and the amount of fibroblast cells in the incision wound healing process that induced with Mauli banana stem extract.

Sign in / Sign up

Export Citation Format

Share Document