scholarly journals Extracellular enzymatic activities of 32 fungal species

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
A. Crippa ◽  
E. Bruno ◽  
A. M. Mangiarotti ◽  
G. Caretta
Keyword(s):  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Aureobasidium Pullulans ◽  
Fungal Species ◽  
Penicillium Digitatum ◽  
Microsporum Gypseum ◽  
Gliocladium Roseum ◽  
Rhizopus Stolonifer ◽  
Penicillium Brevicompactum ◽  
Extracellular Enzymatic Activities

Se investigó en 32 especies fúngicas, la producción de enzimas extracelulares y ms determinaciones cualitativas (amilasa, celulosa, caseinasa, fosfatasa, lipasa, pectinasa y ureasa). La detección de los enzimas en los cultivos fúngicos en agar varió ampliamente.En la mayoría de los cultivos fúngicos se detectaron: amilasas, ureasas, y fosfatasas. Las celulosas se pesquizaron particularmente en cultivo de Aspergillus flavus, A. fumigatus, Cladosporium cladosporioides Penicillium brevicompactum, Rhizopus stolonifer, Scopulariopsis flava y Trichothecium roseum. Penicillium digitatum y P.duclauxi fueron productores de caseinasa en un alto grado. La actividad lipolítica se detectó en cultivos con grasas sintéticas tweens 20, 40, 60, y 80 como sustrato para lipasa. Indicaron actividad lipolítico para estos sustratos A. flavus, Aureobasidium pullulans, C. cladosporioides, Gliocladium roseum, Mycothypha microspora, Microsporum gypseum, P. brevicompactum y Scopulariopsis brumptii. Productores de pectinasas fueron las cepas de A. flavus, C. cladosporioides y Fusarium oxysporum, P. brevicompoctum y Ulocladium atrum

scholarly journals Qualidade sanitária e fisiológica de sementes de abóbora variedade Menina Brasileira

2006 ◽  
Vol 31 (2) ◽  
pp. 158-163 ◽  
Author(s):  
Derblai Casaroli ◽  
Danton C. Garcia ◽  
Marlove F. B. Muniz ◽  
Nilson L. de Menezes
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Alternaria Alternata ◽  
Penicillium Digitatum ◽  
Cucurbita Moschata ◽  
Rhizopus Stolonifer ◽  
Cladosporium Cucumerinum

O trabalho teve como objetivos avaliar e correlacionar a qualidade sanitária e fisiológica de sementes de abóbora, variedade Menina Brasileira (Cucurbita moschata.). Foram avaliados dois lotes de sementes de abóbora produzidas no sistema agroecológico e quatro no sistema convencional, com e sem tratamento químico. Os lotes foram submetidos aos testes de sanidade, seguindo a metodologia do "Blotter test", com congelamento, germinação e vigor (primeira contagem, índice de velocidade de germinação, envelhecimento acelerado e emergência de plântulas). Os resultados indicaram a separação dos lotes de diferentes origens a partir da qualidade sanitária e fisiológica, onde as maiores incidências de fungos foram observadas nos lotes agroecológicos e o maior potencial fisiológico foi observado nos lotes de origem convencional não tratados. Foram encontrados os fungos Fusarium oxysporum, Alternaria alternata, Cladosporium cucumerinum, Aspergillus niger, Penicillium digitatum, Rhizopus stolonifer e Phoma terrestris. A qualidade sanitária não interferiu na qualidade fisiológica das sementes de abóbora, variedade Menina Brasileira.

scholarly journals Phylloplane mycoflora of Gerbera spp. and their Pathogenic potentiality

2014 ◽  
Vol 37 (2) ◽  
pp. 211-217 ◽  
Author(s):  
Fatema Yeasmin ◽  
Shamim Shamsi
Keyword(s):  
Aspergillus Flavus ◽  
New Record ◽  
Trichoderma Viride ◽  
Fungal Species ◽  
Rhizopus Stolonifer ◽  
Penicillium Sp ◽  
Colletotrichum Spp ◽  
Chaetomella Raphigera ◽  
Fusarium Nivale ◽  
Academy Of Sciences

Two types of symptoms, blight and anthracnose, were recorded on two species of Gerbera viz. Gerbera aurantiaca L. and Gerbera viridifolia L. The most common fungal species isolated from Gerbera spp. were Alternaria citrii, A. tennuisssima, Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Bipolaris hawaiiensis, Chaetomella raphigera, Cladosporium cladosporoides, Colletotrichum capsici, C. coffeanum, C. dematium, C. gleosporioides, C. lindemuthianum, C. musae, C. orbiculare, Colletorichum sp.1, Colletotrichum sp.2, Colletotrichum sp.3, Colletotrichum sp.4, Curvularia clavata, C. lunata, Fusarium nivale, F. semitectum, Phomopsis sp., Penicillium sp., Pestalotia sp., Rhizopus stolonifer, Trichoderma viride, one cleistothecial ascomycetes and one sterile fungus. The frequency percentage of association of Colletotrichum spp. with Gerbera spp. was higher than any other fungi. Bipolaris hawaiiensis is a new record for Bangladesh. Among the isolated fungi Alternaria citrii, A. tennuissima, Colletotrichum capcisi, C. dematium, C. coffeanum and Curvularia clavata were found to be pathogenic to Gerbera plant. DOI: http://dx.doi.org/10.3329/jbas.v37i2.17562 Journal of Bangladesh Academy of Sciences, Vol. 37, No. 2, 211-217, 2013

scholarly journals Isolation and Identification of Soil Mycoflora in the Upland and Lowland Soils of Usmanu Danfodiyo University, Sokoto, Sokoto State

2018 ◽  
pp. 1-7
Author(s):  
I. Y. Tafinta ◽  
K. Sheh ◽  
H. M. Maishanu ◽  
S. S. Noma ◽  
S. A. Yusif ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Fungal Species ◽  
Potato Dextrose Agar ◽  
Morphological Features ◽  
Rhizopus Stolonifer ◽  
Isolation And Identification ◽  
Dilution Plate ◽  
Cotton Blue

A total of 14 different fungal species belonging to 7 genera were isolated from the upland and lowland soils of Usmanu Danfodiyo University, Sokoto, between October to December, 2015. The mycoflora were isolated using dilution plate technique on Potato dextrose agar amended by 1% streptomycin. Identification was made microscopically using the lacto-phenol cotton blue method and macroscopically by comparing the cultural and morphological features with the help of authentic fungal manual and taxonomic key. The identified species are; Aspergillus niger, A. flavus, A. fumigatus, A. nidulans, A. oryzae, Alternaria longifes, Fusarium oxysporum, F. solani, F. mangifera, Rhizopus stolonifer, R. orizae, Saccharomyces cerevisae, Trichoderma harzianum and Trichophytum quallinum. The highest number of fungi (50 isolates) were obtained from lowland uncultivated soils (D), followed by the upland uncultivated soils (B) with about 34 strains, and the least (22 isolates) were obtained from upland cultivated soils (A) out of the total 132 isolates. Variations between sites were statistically analyzed.

scholarly journals Fungi causing rots in onions at storage and market

1970 ◽  
Vol 6 (2) ◽  
pp. 245-251 ◽  
Author(s):  
MAM Ara ◽  
ML Khatun ◽  
M Ashrafuzzaman
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Fusarium Moniliforme ◽  
Room Temperature ◽  
Storage Period ◽  
Fungal Species ◽  
Disease Development ◽  
Duration Of Storage ◽  
Penicillium Spp

Five varieties of diseased onion samples, four local (Taherpuri, Faridpuri, Kalashnagari, and Zitka) and one Indian (Pusa Red) were collected from different markets of Mymensingh, to isolate and identify the associated fungi. Five different fungal species belonging to three genera were repeatedly isolated. Healthy looking and diseased onions were kept at room temperature. The population of associated fungi increased proportionately with the duration of storage period. The isolated fungi were Aspergillus niger, Aspergillus flavus, Penicillium spp., Fusarium oxysporum and Fusarium moniliforme. Healthy onion bulbs were inoculated (with injury and without injury) with these fungi. Individual fungi produced distinguishable symptom of disease in incubation chamber at 22-23°C temperature. After 30 days of inoculation, bulbs showed comparatively low amount of disease development than those at 60 and 90 days. Maximum disease development was found in the variety Pusa Red (with injury) and it was minimum in Zitka variety (without injury). Keywords: Fungi; Onion; Disease development DOI: 10.3329/jbau.v6i2.4818 J. Bangladesh Agril. Univ. 6(2): 245-251, 2008

scholarly journals Fungi associated with anthracnose of mango (Mangifera indica L.) fruits and their pathogenic potentiality

2018 ◽  
Vol 27 (1) ◽  
pp. 93-100
Author(s):  
Farhana Islam ◽  
Shamim Shamsi ◽  
Md Abul Bashar
Keyword(s):  
Aspergillus Flavus ◽  
Alternaria Alternata ◽  
New Record ◽  
Colletotrichum Gloeosporioides ◽  
Mangifera Indica ◽  
Fungal Species ◽  
Rhizopus Stolonifer ◽  
Penicillium Sp ◽  
Fusarium Semitectum

A total of ten fungal species were isolated from the anthracnose symptoms of three varieties of Mangifera indica L. viz., Himsagar, Langra and Amrapali. The isolated fungi were Alternaria alternata (Fr.) Keissler, Aspergillus flavus Link, A. fumigatus Fresenius, A. niger van Tiegh., Colletotrichum gloeosporioides (Penz.) Sacc., Fusarium semitectum Berk. & Rav., Papulaspora sp. Preuss., Penicillium sp. Link., Pestalotiopsis guepinii (Desm.) Stey. and Rhizopus stolonifer (Ehrenb.) Vull. Colletotrichum gloeosporioides showed the highest per cent (26.82) frequency in Himsagar whereas R. stolonifer showed the lowest (2.22). Pestalotiopsis guepinii showed the highest per cent (46.83) frequency in Langra whereas R. stolonifer showed the lowest (1.17). Colletotrichum gloeosporioides showed the highest per cent (38.67) frequency in Amrapali whereas Papulaspora sp. showed the lowest (4.00). Among the isolated fungi C. gloeosporioides, F. semitectum and P. guepinii were found to be pathogenic to three mango varieties. Association of Fusarium semitectum, Papulaspora sp. and Pestalotiopsis guepinii with mango is new record in Bangladesh. Dhaka Univ. J. Biol. Sci. 27(1): 93-100, 2018 (January)

scholarly journals Mycoflora Associated with Decomposition of Rice Stubble Mixed with Soil

2008 ◽  
Vol 48 (2) ◽  
pp. 247-254 ◽  
Author(s):  
Vibha Pandey ◽  
Asha Sinha
Keyword(s):  
Moisture Content ◽  
Aspergillus Flavus ◽  
Trichoderma Harzianum ◽  
Climatic Factors ◽  
Alternaria Solani ◽  
Fungal Species ◽  
Monthly Variation ◽  
Rhizopus Stolonifer ◽  
Substrate Availability ◽  
Fungal Population

Mycoflora Associated with Decomposition of Rice Stubble Mixed with SoilFungi inhabiting rice stubble mixed with soil were isolated by dilution plate technique. Moisture content of decomposed rice stubble mixed with soil was 25.32% in the month of August and minimum (5.35%) in the month of April and pH varied between 6.8-7.2. The highest (47.68 x 104) fungal population in 1 g of rice stubble mixed with soil was recorded in the month of October and the lowest (16.88 x 104) in the month of May. A total of twenty-nine fungal species were recorded during the entire period of decomposition. Dueteromycetous fungi constituted 75.86 per cent of total fungal population followed by zygomycetous, oomycetous and ascomycetous fungi. Monthly variation in fungal population was correlated with substrate availability and climatic factors. Dominance ofRhizopus stolonifer, Aspergillus flavusandTrichoderma harzianumwas recorded during the decomposition whilePestalotia mangiferae, Torula graminisandAlternaria solaniwere obtained as rare fungal species.

scholarly journals Application of clove and dill oils as an alternative of salphos for chickpea food seed storage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Narendra Kumar ◽  
S. M. Paul Khurana ◽  
Vashist N. Pandey
Keyword(s):  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Chemical Properties ◽  
Seed Storage ◽  
Fungal Species ◽  
Complete Inhibition ◽  
Grocery Stores ◽  
Callosobruchus Chinensis ◽  
Fungicidal Action ◽  
Growth Of Fungi

AbstractMycological investigations of 25 samples of stored chickpea food seeds (Cicer arietinum L.) from grocery stores of Gurgaon and Gorakhpur revealed occurrence of seventeen fungal species belonging to genus viz., Alternaria, Aspergillus, Chaetomium, Colletotrichum, Curvularia, Fusarium, Penicillium, Rhizopus, Rhizoctonia, and Sclerotium. In these Aspergillus flavus, A. niger, Fusarium oxysporum had dominance in terms of per cent occurrence. Only one species of Bruchid (Callosobruchus chinensis L.) occurred in all the 25 samples. The biodeterioration of seeds inoculated with fungi: A. flavus, A. niger, F. oxysporum and the insect—C. chinensis, revealed their role in seed deterioration. For chickpea food seed protection essential oils were extracted from edible commodity(clove(Lavang and dill(sowa) leaf). Clove(Lavang) oil registered highest antifungal activity inhibiting (100%) mycelial growth of fungi, viz. species Aspergillus flavus, A. niger, Fusarium oxysporum at 300 ppm but was fungicidal at 400 ppm. Dill (Sowa) oil showed complete inhibition at 400 ppm and was fungicidal at 500 ppm. While mixture of both the oils (clove and dill) showed complete inhibition (100%) and fungicidal action at 400 ppm against the dominant fungi. The oils showed 100% insect repellent activity and were found fungicidal at 0.02 ml dose and also insecticidal. The mixture of oils was cidal at 0.02 ml dose. The mixture of oils showed a broad antifungal spectrum at 500 ppm while only 70–93% inhibitory activity at 300 ppm. The oils' mixture's activity was not affected by temp, storage and autoclaving up to 150 days. Oils physico-chemical properties were studied. GC–MS analysis of clove(Lavang) oil depicted major components: 75.63%eugenol while dill(sowa) leaf oil had 25.14% apiole. Formulation of Mixture of oils was more effective showing complete seed protection i.e.no growth of fungi and insects upto 150 days storage than salphos (150 days). While salphos controlled only maximum three fungi (A. terreus, C. dematium, F. moniliforme). The formulated oils mixture did not have any adverse effect on the chickpea seeds and increased their shelf life.

scholarly journals In Vitro Studies of Antifungal Activity of Colloidal Silver against Important Plants Pathogens

2018 ◽  
Vol 46 (2) ◽  
pp. 533-537 ◽  
Author(s):  
Oana VENAT ◽  
Beatrice IACOMI ◽  
Adrian G. PETICILĂ
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Botrytis Cinerea ◽  
Aspergillus Flavus ◽  
Mycelial Growth ◽  
Fusarium Culmorum ◽  
Penicillium Digitatum ◽  
Alternaria Brassicicola ◽  
Colloidal Silver

Colloids and especially silver are increasingly used in a variety of worldwide applications because of their potential antimicrobial activity and their plasmotic and conductivity properties. This research reports the fungitoxic properties of colloidal silver on mycelial growth of important plant pathogens: Alternaria brassicicola, Botrytis cinerea, Aspergillus flavus, Aspergillus niger, Fusarium culmorum, Fusarium oxysporum, Penicillium digitatum and Sclerotinia sclerotiorum. Although variable responses towards each compound were observed within the species the results revealed a clear reaction to limiting mycelium growth relative to various concentration of Colloidal silver (CS). Results were expressed as effective concentrations which inhibit mycelial growth by 50% and 90% respectively (EC50 and EC90). Efficiency of colloidal silver on mycelial growth inhibition of different isolates based on EC50 have the following values: 3.69 ppm for Alternaria brassicicola, 7.32 ppm for Botrytis cinerea, 18.21 ppm for Aspergillus flavus, 10.43 ppm for Aspergillus niger, 11.99 ppm for Fusarium culmorum, 12.27 ppm for Fusarium oxysporum, 10.82 ppm for Penicillium digitatum and 6.34 ppm for Sclerotinia. According to the obtained results the antifungal activity of colloidal silver particles as biocide has potential for using it as a non-aggressive treatment in horticulture and sustainable horticulture.

scholarly journals Discrimination of Aspergillosis, Mucormycosis, Fusariosis, and Scedosporiosis in Formalin-Fixed Paraffin-Embedded Tissue Specimens by Use of Multiple Real-Time Quantitative PCR Assays

2016 ◽  
Vol 54 (11) ◽  
pp. 2798-2803 ◽  
Author(s):  
Elham Salehi ◽  
Mohammad T. Hedayati ◽  
Jan Zoll ◽  
Haleh Rafati ◽  
Maryam Ghasemi ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Ffpe Tissue ◽  
Content Type ◽  
Real Time Quantitative Pcr ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Tissue Specimens ◽  
Formalin Fixed

In a retrospective multicenter study, 102 formalin-fixed paraffin-embedded (FFPE) tissue specimens with histopathology results were tested. Two 4- to 5-μm FFPE tissue sections from each specimen were digested with proteinase K, followed by automated nucleic acid extraction. Multiple real-time quantitative PCR (qPCR) assays targeting the internal transcribed spacer 2 (ITS2) region of ribosomal DNA, using fluorescently labeled primers, was performed to identify clinically important genera and species of Aspergillus , Fusarium , Scedosporium , and the Mucormycetes . The molecular identification was correlated with results from histological examination. One of the main findings of our study was the high sensitivity of the automated DNA extraction method, which was estimated to be 94%. The qPCR procedure that was evaluated identified a range of fungal genera/species, including Aspergillus fumigatus , Aspergillus flavus , Aspergillus terreus , Aspergillus niger , Fusarium oxysporum , Fusarium solani , Scedosporium apiospermum , Rhizopus oryzae , Rhizopus microsporus , Mucor spp., and Syncephalastrum . Fusarium oxysporum and F. solani DNA was amplified from five specimens from patients initially diagnosed by histopathology as having aspergillosis. Aspergillus flavus , S. apiospermum , and Syncephalastrum were detected from histopathological mucormycosis samples. In addition, examination of four samples from patients suspected of having concomitant aspergillosis and mucormycosis infections resulted in the identification of two A. flavus isolates, one Mucor isolate, and only one sample having both R. oryzae and A. flavus . Our results indicate that histopathological features of molds may be easily confused in tissue sections. The qPCR assay used in this study is a reliable tool for the rapid and accurate identification of fungal pathogens to the genus and species levels directly from FFPE tissues.

scholarly journals Phytochemical screening and antifungal potentials of Citrus limon peels against Fusarium oxysporum and Rhizopus stolonifer causing rots in water melon (Citrullus lanatus L.)

2020 ◽  
pp. 17-21
Author(s):  
L.U. Bashir ◽  
A. Abdulkadir ◽  
M. M. Shah ◽  
A. Hamisu ◽  
U. Sharif ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Citrullus Lanatus ◽  
Citrus Limon ◽  
Pathogenicity Test ◽  
Phytochemical Screening ◽  
Rhizopus Stolonifer ◽  
Methanol Extracts ◽  
Antifungal Potential ◽  
Phytochemical Constituents ◽  
Water Melon

This study investigated the phytochemical constituents and antifungal potential of Citrus limon peels using aqueous and methanol extracts against Fusarium oxysporum and Rhizopus stolonifer causing rots in water melon. The result of phytocemical screening revealed the presence of Saponin, Tannin, Phenols, Alkaloid and Flavanoid. Pathogenicity test conducted showed that F. oxysporum and R. stolonifer causes rots of Citrullus lanatus. The extracts inhibited the growth of F. oxysporum (76.67% and 60.00 %) methanol and aqueous respectively. R. stolonifer growth was inhibited (85.93% and 71.11%) methanol and aqueous extracts. The fungitoxicity of synthetic fungicide Benomyl was 95.56%. The methanol extracts has highest inhibitory activity than aqueous extract. The result of the findings indicates the Biofungicide potentials of Citrus limon peels as alternative to synthetic fungicide in management of phyto-pathogens of water lemon.

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