Moleculr characterization of antibiotic resistant gene of Staphylococcus aureus isolated from hospitals

2017 ◽  
Vol 8 (3) ◽  
Author(s):  
HUSSEIN HAMEED ◽  
JASIM MOHAMMED SADEQ
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistant ◽  
Resistant Gene

scholarly journals Genetic Relatedness and Diversity of Staphylococcus aureus from Different Reservoirs: Humans and Animals of Livestock, Poultry, Zoo, and Aquaculture

2020 ◽  
Vol 8 (9) ◽  
pp. 1345
Author(s):  
Vanessa Salgueiro ◽  
Vera Manageiro ◽  
Narcisa M. Bandarra ◽  
Eugénia Ferreira ◽  
Lurdes Clemente ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Relatedness ◽  
Meca Gene ◽  
Resistance Mechanisms ◽  
Animal Origin ◽  
Antibiotic Resistant ◽  
Typing Methods ◽  
Phylogenetic Lineages ◽  
Spa Types

The main aim of this study was the characterization of antibiotic resistance mechanisms in 82 Staphylococcus aureus strains isolated from humans and animals. Antibiotic susceptibility testing was performed on all S. aureus isolates accordingly, and antibiotic-resistant genes were investigated by genotypic methods. The genetic diversity of S. aureus was studied through spa, multilocus sequence typing (MLST), and agr typing methods. The majority of S. aureus from human sources were resistant to cefoxitin (and harbor the mecA gene) and fluoroquinolones, whereas only four strains of S. aureus from animal sources revealed resistance to ciprofloxacin. In the set of S. aureus isolated from humans, the most frequent spa, MLST, and agr group were t032, ST22, and I, respectively. In strains from animal origin the most common spa, MLST, and agr group found were t2383, ST398, and III/not typable, respectively. S. aureus from humans and animals were identified either in clonal complexes CC5, CC30, and CC398, suggesting that they have the same putative founder in their evolution. Considering the three CCs encompassing strains from human and animal reservoirs with different spa-types, we can hypothesize that this might reflect an adaptation to different phylogenetic lineages in those reservoirs (host species) probably associated to genetic diversification of pre-existing strains.

scholarly journals Isolation and Genetic Characterization of Vancomycin-resistant and mecC+ Methicillin-resistant Staphylococcus aureus Strains in Clinical Samples of Bojnurd, Northeastern Iran

2021 ◽  
Vol 14 (10) ◽  
Author(s):  
Parastoo Zarghami Moghaddam ◽  
Amir Azimian ◽  
Abbas Akhavan Sepahy ◽  
Alireza Iranbakhsh
Keyword(s):  
Staphylococcus Aureus ◽  
Tertiary Hospital ◽  
Clinical Samples ◽  
Spa Typing ◽  
Antibiotic Resistant ◽  
Methicillin Resistant ◽  
Alpha Hemolysin ◽  
Pcr Method ◽  
Vancomycin Resistant

Background: The emergence of antibiotic-resistant Staphylococcus aureus strains is one of the major concerns about the various staphylococcal infections. Vancomycin is one the most important effective antibiotics on staphylococcal lethal infections. To date, vancomycin-resistant strains are increasingly isolated in different parts of the world, and it is alerting. Objectives: The current study was designed to evaluate the prevalence, and antibiotic susceptibility pattern of methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S. aureus (VRSA) isolates in the main tertiary hospital of Bojnurd, Iran. Methods: S. aureus isolates were collected from different clinical samples in Imam Reza Hospital of Bojnurd. After identification of isolates through using conventional methods, they were evaluated by agar screening, disk diffusion, and minimum inhibitory concentration (MIC) methods to determine resistance to vancomycin and methicillin. We also performed polymerase chain reaction (PCR) for the detection of mecA, mecC, vanA, and vanB genes. After confirmation of vancomycin resistance, genetic analysis was performed using SCCmec, agr, and spa typing, and multilocus sequence typing (MLST) methods on VRSA isolates. Results: We found four vancomycin-resistant isolates (1.29%). Also, 75% of isolates were resistant to cefoxitin. Using the PCR method, mecA was found in 73%, mecC in 0.64%, and vanA in 1.29% of isolates. Interestingly, we found two mecC positive isolates in MRSA isolates. The alpha-hemolysin (81.81%) and enterotoxin C (27%) had the highest and lowest toxins percentage, respectively. Among mecA positive isolates, SCCmecIV (37%), SCCmecIII (31.27%), SCCmecI (14%), SCCmecII (11%), and SCCmecV (5.7%) were the most prevalent SCCmec types, respectively. It should be noted that the two mecC positive isolates belonged to SCCmecXI. AgrI (76.29%) was the highest agr type. We recognized t037 as the dominant spa type, and ST239, ST6, ST97, and ST8 were found in VRSA isolates. Conclusions: In our study, the frequency of mecA genes in MRSA isolates was very high. It seems that the resistant isolates belonged to endemic clones of Iran.

scholarly journals Antibiotic Resistant Pattern and Resistant Gene Identification of Staphylococcus aureus from Chicken Farm in Bogor

2021 ◽  
Vol 22 (2) ◽  
pp. 262-270
Author(s):  
Nabila Swarna Puspa Hermana ◽  
Usamah Afiff ◽  
Safika Safika ◽  
Agustin Indrawati ◽  
Fachriyan Hasmi Pasaribu
Keyword(s):  
Staphylococcus Aureus ◽  
Cloacal Swab ◽  
Resistance Pattern ◽  
Poultry Farm ◽  
Drug Resistant ◽  
Antibiotic Resistant ◽  
Mannitol Salt Agar ◽  
Resistant Gene ◽  
Ermb Gene ◽  
Layer Farm

Chicken is one of the important protein source in Indonesia. Moreover, the largest population of chicken layer and poultry in Indonesia is known situated at West Java province with Bogor manicipality as the main producer. The aims of this study were to determine the antibiotic resistance pattern of Staphylococcus aureus isolated from poultry and layer farm in Bogor. The study also identified gene encoded the resistance. Cloacal swab samples were collected from chicken broiler and layer farm in Bogor manicipality. The samples were then cultured in Mannitol Salt Agar (MSA) medium to obtain S. aureus. Suspected colony was then confirmed by biochemical test. Positive strains were tested against several antibiotics and the diameter of clear zone arround of colony was interpreted based on Clinical and Laboratory Standard Institute. Furthermore, the DNA from resistant strains were then extracted, followed by detection of the resistance gene by using polymerase chain reaction (PCR) method. A total of 14 isolates of S. aureus were positive from poultry farm, and 15 isolates from layer farm. Most of all were resistant to tetracycline, ampicillin, oxytetracycline, erythromycin and nalidixic acid. On the other hands, several strains were sensitive to gentamycin and chloramphenicol. The study showed 28 isolates out of them were multi-drug resistant. Resistant gene such as blaTEM, gyrA and tetA were also identified in some isolates except for ErmB gene which was found in isolates originated from poultry farm. In conclussion, S. aureus in both farm showed mostly multi-drug resistant to several antibiotics which were supported by identification of resistant gene among isolates.

scholarly journals Molecular characterization of antibiotic-resistant Staphylococcus aureus from livestock (bovine and swine)

2017 ◽  
Vol 10 (6) ◽  
pp. 598-604 ◽  
Author(s):  
Asima Zehra ◽  
Randhir Singh ◽  
Simranpreet Kaur ◽  
J. P. S. Gill
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Antibiotic Resistant

Characterization of antibiotic resistant Staphylococcus aureus isolates from ready-to-eat foods in Korea

2014 ◽  
Vol 57 (3) ◽  
pp. 387-395 ◽  
Author(s):  
Kyung Min Park ◽  
Su Kyung Oh ◽  
Jeong-Ok Cha ◽  
Yoeng Seon Lee ◽  
Minseon Koo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistant

Antibiotic Susceptibility of Bacteria Isolated from Pasteurized Milk and Characterization of Macrolide-Lincosamide-Streptogramin Resistance Genes

2005 ◽  
Vol 68 (2) ◽  
pp. 347-352 ◽  
Author(s):  
AGNÈS PERRIN-GUYOMARD ◽  
CHRISTOPHE SOUMET ◽  
ROLAND LECLERCQ ◽  
FLORENCE DOUCET-POPULAIRE ◽  
PASCAL SANDERS
Keyword(s):  
Staphylococcus Aureus ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Selective Media ◽  
Pasteurized Milk ◽  
Gram Positive Bacteria ◽  
Milk Samples

The presence of antibiotic-resistant bacteria in pasteurized milk was detected by plating 18 milk samples on selective media containing β-lactams, macrolides, or a glycopeptide. Most samples contained gram-positive bacteria that grew on agar plates containing oxacillin, erythromycin, and/or spiramycin. The disk-diffusion method confirmed resistance to erythromycin and/or spiramycin in 86 and 65% of the coryneform bacteria and Micrococcaceae tested, respectively. PCR and sequence analysis revealed the presence of an ermC gene in 2 of the 25 Micrococcaceae strains investigated for their resistance to erythromycin and/or spiramycin. None of the 14 corynebacteria strains resistant to erythromycin and/or spiramycin harbored the erm(X) gene. No gene transfer could be demonstrated between the two erm(C) staphylococcal isolates and recipient strains of Enterococcus faecalis JH2-2 or Staphylococcus aureus 80CR5.

Sign in / Sign up

Export Citation Format

Share Document