Investigation of the presence of biofilm in Bacillus subtilis, Bacillus licheniformis and Bacillus cereus which are isolated from raw milks

2013 ◽  
Vol 24 ◽  
pp. S101-S102 ◽  
Author(s):  
Güven Uraz ◽  
Seda Teke Gündüz
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacillus Licheniformis

Detection and quantification of spoilage and pathogenic Bacillus cereus, Bacillus subtilis and Bacillus licheniformis by real-time PCR

2011 ◽  
Vol 28 (3) ◽  
pp. 605-610 ◽  
Author(s):  
I.C. Fernández-No ◽  
M. Guarddon ◽  
K. Böhme ◽  
A. Cepeda ◽  
P. Calo-Mata ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Real Time ◽  
Bacillus Licheniformis ◽  
Real Time Pcr ◽  
Detection And Quantification

scholarly journals RECONSTITUTION OF DIFFERENT SIGNAL PEPTIDES FOR ENHANCED THERMOSTABLE ALPHA AMYLASE SECRETION IN Bacillus subtilis

2018 ◽  
Vol 56 (1) ◽  
pp. 7 ◽  
Author(s):  
Nguyen Thị Da ◽  
Tran Dinh Man ◽  
Nguyen Kim Thoa
Keyword(s):  
Molecular Weight ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacillus Licheniformis ◽  
Signal Peptide ◽  
Amylase Activity ◽  
Alpha Amylase ◽  
Amylase Secretion ◽  
Signal Peptides ◽  
Extracellular Amylase

Three signal peptides of alpha amylase genes of three isolated strains that were Bacillus licheniformis DA23, Bacillus subtilis D5-2, Bacillus cereus CN1-5 were successfully sequenced. Three predicted “Sec – type” signal peptides have a length varying from 27 (CN1-5) to 33 residues (D5-2).  The secretion of alpha amylase of the recombination B. subtilis 168MPgrac strain (pHV33–PgracAmy3BT2) with 71.4U/ml was larger than that of 168MPamy with 53.2U/ml. Base on analyzed rerults of PAGE and zymogram about molecular weight, alpha amylases in both strains were the same size, nearly 58kDa. The extracellular amylase activity of signal peptide SsubtilisD5.2 in 168M was highest with 76.4±4.3 U/ml in four signal peptide targets.  

Characterisation and profiling of Bacillus subtilis, Bacillus cereus and Bacillus licheniformis by MALDI-TOF mass fingerprinting

2013 ◽  
Vol 33 (2) ◽  
pp. 235-242 ◽  
Author(s):  
I.C. Fernández-No ◽  
K. Böhme ◽  
M. Díaz-Bao ◽  
A. Cepeda ◽  
J. Barros-Velázquez ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacillus Licheniformis ◽  
Maldi Tof

scholarly journals Evaluación del crecimiento de cuatro especies del género Bacillus sp., primer paso para entender su efecto biocontrolador sobre Fusarium sp.

Nova ◽  
2016 ◽  
Vol 14 (26) ◽  
pp. 53 ◽  
Author(s):  
Estefania Castañeda Alvarez ◽  
Ligia Consuelo Sánchez
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Bacillus Licheniformis ◽  
Bacillus Sp ◽  
Fusarium Sp

<p>Evaluar las condiciones de crecimiento de cuatro especies de <em>Bacillus </em>sp. nativas a escala de 10ml en Medio Mínimo de Sales (MMS) como primer paso para entender su acción biocontroladora contra <em>Fusarium </em>sp. <strong>Métodos. </strong>El procedimiento para evaluar el crecimiento de los aislamientos UCMC-TB1, UCMC-TB2, UCMC-TB3 y UCMC-TB4 se realizó utilizando espectrofotometría y recuento directo en placa y pruebas de antagonismo dual en placa para evaluar el efecto controlador contra <em>Fusarium </em>sp. <strong>Resultados. </strong>Se confirmó la identificación por pruebas bioquímicas de los cuatro aislamientos: <em>Bacillus licheniformis, Bacillus subtilis, Bacillus </em><em>pumilus y Bacillus cereus; </em>todas las cepas presentaron antagonismo <em>in vitro</em>. El <em>Bacillus subtilis </em>fue la especie que demostró mayor capacidad antagónica (79,73%PICR) y las características más destacadas de esta cepa fueron su velocidad de crecimiento. El género <em>Bacillus </em>es uno de los más reportados para usar en el control biológico de hongos como <em>Fusarium </em>sp. el cual ataca un gran número de cultivos de interés económico para el sector agrícola en Colombia.</p>

Probiotics in the diets of laying quails

2020 ◽  
pp. 60-66
Author(s):  
O. Merzlyakova ◽  
V. Rogachyev ◽  
V. Chegodaev
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Egg Production ◽  
Positive Impact ◽  
Hematological Parameters ◽  
Economic Effect ◽  
Egg Laying ◽  
Control Group ◽  
Egg Mass ◽  
The Cost

The efficiency of introducing probiotics based on strains of Bacillus subtilis, Bacillus licheniformis and their consortium in the amount of 150 g/t of feed into the diets of laying quails has been studied. The experiment lasting 182 days has been carried out on four groups of quails with 30 heads in each. The quails have been housed in the broiler battery in compliance with the required microclimate conditions. Quails of all groups have been received the main diet (compound feed) developed taking into account their age and physiological characteristics. The quails of the 1st, 2nd and 3rd experimental groups in addition to the main diet received probiotics (150 g/t compound feed) based on strains Bacillus subtilis, Bacillus licheniformis and their consortium, respectively. It has been found that feeding the laying quails of the consortium of strains Bacillus subtilis and Bacillus licheniformis had the most significant positive impact on their productive performance, it allowed to increase egg production by 7,81 %, egg laying intensity by 5,0 %, egg mass yield by 9,77 %, while reducing feed expenditures for 10 eggs by 13,35 %. The yield of hatching eggs has been increased by 7,03 %, hatchability of chickens from laid and fertilized eggs by 8,33 and 8,35 %, brooding waste decreased by 21,74 %. Hematological parameters of quails during the whole experiment were within the physiological norm. The economic effect calculated on the basis of data on the cost of compound feed, probiotics and the cost of sold eggs of quail laying was 14,56 % in the 3rd experimental group (in relation to the control group).

scholarly journals Effect of BioPlus YC Probiotic Supplementation on Gut Microbiota, Production Performance, Carcass and Meat Quality of Pigs

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1581
Author(s):  
Artur Rybarczyk ◽  
Elżbieta Bogusławska-Wąs ◽  
Alicja Dłubała
Keyword(s):  
Bacillus Subtilis ◽  
Meat Quality ◽  
Bacillus Licheniformis ◽  
Average Daily Gain ◽  
Production Performance ◽  
Probiotic Bacteria ◽  
Control Group ◽  
Production Traits ◽  
Feed Conversion

The objective of the study was to determine the effects of probiotic bacteria Bacillus licheniformis and Bacillus subtilis on microbiological properties of feed mixtures and on the digestive tract content as applicable to production traits and carcass characteristics of fatteners. The experiment was performed on 83,838 fatteners from four successive (insertions) productions in two groups. From the seventy eighth day of age till marketing to the slaughter plant, the pigs were supplied with BioPlus YC probiotic (Chr. Hansen) in the amount of 400 g/t. The preparation contained a complex of probiotic bacteria Bacillus licheniformis DSM 5749, and Bacillus subtilis DSM 5750 spores in a 1:1 ratio. From the fourth insertion, after reaching a body weight of approximately 112 kg, 60 fatteners were selected from each group to measure carcass quality and half of them for meat quality evaluation. Moreover, microbiological analyses in feed and colon were performed. The study showed that BioPlus YC probiotics supplementation resulted in a significantly higher count of B. subtilis and B. licheniformis in the feed, a higher count of B. subtilis, B. licheniformis and LAB, as well as a lower count of Enterobacteriaceae, Enterococcus, Clostridium and Bacillus sp. in the mucosa and in the colorectal content of the test pigs. Our work has shown that supplementation with the BioPlus YC probiotic had a positive effect on the production traits of pigs mainly by reducing mortality (2.83%, p = 0.010), lowering feed conversion ratio—FCR (2.59 kg/kg, p = 0.013), better average daily gain—ADG (0.95 kg/day, p = 0.002) and shorter fattening period (77.25 days, p = 0.019) when compared to the control group (4.19%; 2.79 kg/kg; 0.89 kg/day; 92.8 days, respectively). The addition of the specific Bacillus bacteria did not influence carcass and meat characteristics of the test fatteners.

scholarly journals Intracellular localization of the mycobacterial stressosome complex

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Malavika Ramesh ◽  
Ram Gopal Nitharwal ◽  
Phani Rama Krishna Behra ◽  
B. M. Fredrik Pettersson ◽  
Santanu Dasgupta ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Fluorescence Microscopy ◽  
Bacillus Cereus ◽  
Intracellular Localization ◽  
Sigma Factors ◽  
Alternative Sigma Factors ◽  
Expression Of Genes ◽  
Molecular Machinery ◽  
Stress Signals ◽  
Activation Pathways

AbstractMicroorganisms survive stresses by alternating the expression of genes suitable for surviving the immediate and present danger and eventually adapt to new conditions. Many bacteria have evolved a multiprotein "molecular machinery" designated the "Stressosome" that integrates different stress signals and activates alternative sigma factors for appropriate downstream responses. We and others have identified orthologs of some of the Bacillus subtilis stressosome components, RsbR, RsbS, RsbT and RsbUVW in several mycobacteria and we have previously reported mutual interactions among the stressosome components RsbR, RsbS, RsbT and RsbUVW from Mycobacterium marinum. Here we provide evidence that "STAS" domains of both RsbR and RsbS are important for establishing the interaction and thus critical for stressosome assembly. Fluorescence microscopy further suggested co-localization of RsbR and RsbS in multiprotein complexes visible as co-localized fluorescent foci distributed at scattered locations in the M. marinum cytoplasm; the number, intensity and distribution of such foci changed in cells under stressed conditions. Finally, we provide bioinformatics data that 17 (of 244) mycobacteria, which lack the RsbRST genes, carry homologs of Bacillus cereus genes rsbK and rsbM indicating the existence of alternative σF activation pathways among mycobacteria.

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