scholarly journals Waste fish oil as an alternative carbon source in microbial oil production by Yarrowia lipolytica yeast

2019 ◽  
pp. 3-13
Author(s):  
Agata Fabiszewska ◽  
Patrycja Mazurczak-Zieniuk ◽  
Dorota Nowak ◽  
Małgorzata Wołoszynowska ◽  
Bartłomiej Zieniuk
Keyword(s):  
Carbon Source ◽  
Fish Oil ◽  
Yarrowia Lipolytica ◽  
Oil Production ◽  
Microbial Oil ◽  
Alternative Carbon Source

scholarly journals Aeration and Stirring in Yarrowia lipolytica Lipase Biosynthesis during Batch Cultures with Waste Fish Oil as a Carbon Source

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 88
Author(s):  
Paulina Snopek ◽  
Dorota Nowak ◽  
Bartłomiej Zieniuk ◽  
Agata Fabiszewska
Keyword(s):  
Carbon Source ◽  
Fish Oil ◽  
Yarrowia Lipolytica ◽  
Lipolytic Activity ◽  
Culture Conditions ◽  
Agitation Speed ◽  
Oxygen Deficit ◽  
Shear Stresses ◽  
Process Selection ◽  
Batch Cultures

Yarrowia lipolytica is one of the most studied non-conventional forms of yeast, exhibiting a high secretory capacity and producing many industrially important and valuable metabolites. The yeast conceals a great biotechnological potential to synthesize organic acids, sweeteners, microbial oil, or fragrances. The vast majority of bioprocesses are carried out in bioreactors, where suitable culture conditions are provided. In the current study, the effect of agitation speed (200–600 rpm) and air flow rate (0.0375–2.0 dm3/(dm3 × min)) on the biomass yield and lipase activity of Y. lipolytica KKP 379 is analyzed in a growth medium containing waste fish oil. The increase of aeration intensity limited the period of oxygen deficit in the medium. Simultaneously, an increase in lipolytic activity was observed from 2.09 U/cm3 to 14.21 U/cm3; however, an excessive agitation speed likely caused oxidative or shear stresses, and a reduction in lipolytic activity was observed. Moreover, it is confirmed that the synthesis of lipases is related to oxygen consumption, pH, and the yeast growth phase, and appropriate process selection may provide two advantages, namely, the maximum use of the waste carbon source and the production of lipolytic enzymes that are valuable in many industries.

scholarly journals Simultaneous lipid biosynthesis and recovery for oleaginous yeast Yarrowia lipolytica

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Pratik Prashant Pawar ◽  
Annamma Anil Odaneth ◽  
Rajeshkumar Natwarlal Vadgama ◽  
Arvind Mallinath Lali
Keyword(s):  
Yarrowia Lipolytica ◽  
Oil Recovery ◽  
Oleaginous Yeast ◽  
Fermentation Broth ◽  
Continuous Production ◽  
Oil Production ◽  
Biofuel Production ◽  
Oil Yield ◽  
Microbial Oil

Abstract Background Recent trends in bioprocessing have underlined the significance of lignocellulosic biomass conversions for biofuel production. These conversions demand at least 90% energy upgradation of cellulosic sugars to generate renewable drop-in biofuel precursors (Heff/C ~ 2). Chemical methods fail to achieve this without substantial loss of carbon; whereas, oleaginous biological systems propose a greener upgradation route by producing oil from sugars with 30% theoretical yields. However, these oleaginous systems cannot compete with the commercial volumes of vegetable oils in terms of overall oil yields and productivities. One of the significant challenges in the commercial exploitation of these microbial oils lies in the inefficient recovery of the produced oil. This issue has been addressed using highly selective oil capturing agents (OCA), which allow a concomitant microbial oil production and in situ oil recovery process. Results Adsorbent-based oil capturing agents were employed for simultaneous in situ oil recovery in the fermentative production broths. Yarrowia lipolytica, a model oleaginous yeast, was milked incessantly for oil production over 380 h in a media comprising of glucose as a sole carbon and nutrient source. This was achieved by continuous online capture of extracellular oil from the aqueous media and also the cell surface, by fluidizing the fermentation broth over an adsorbent bed of oil capturing agents (OCA). A consistent oil yield of 0.33 g per g of glucose consumed, corresponding to theoretical oil yield over glucose, was achieved using this approach. While the incorporation of the OCA increased the oil content up to 89% with complete substrate consumptions, it also caused an overall process integration. Conclusion The nondisruptive oil capture mediated by an OCA helped in accomplishing a trade-off between microbial oil production and its recovery. This strategy helped in realizing theoretically efficient sugar-to-oil bioconversions in a continuous production process. The process, therefore, endorses a sustainable production of molecular drop-in equivalents through oleaginous yeasts, representing as an absolute microbial oil factory.

Potential of food waste hydrolysate as an alternative carbon source for microbial oil synthesis

2021 ◽  
pp. 126312
Author(s):  
Yaning Xu ◽  
Xuemei Wang ◽  
Zifu Li ◽  
Shikun Cheng ◽  
Jiacheng Jiang
Keyword(s):  
Carbon Source ◽  
Food Waste ◽  
Microbial Oil ◽  
Oil Synthesis ◽  
Alternative Carbon Source

Enhancement of microbial oil production by alpha-linolenic acid producing Yarrowia lipolytica strains QU22 and QU137

2014 ◽  
Vol 23 (6) ◽  
pp. 1929-1934 ◽  
Author(s):  
Paula Mattanna ◽  
Priscila Dallé da Rosa ◽  
Ana Paula Gusso ◽  
Neila S. P. S. Richards ◽  
Patrícia Valente
Keyword(s):  
Linolenic Acid ◽  
Yarrowia Lipolytica ◽  
Oil Production ◽  
Microbial Oil ◽  
Alpha Linolenic Acid

scholarly journals Rhodotorula toruloides Single Cell Oil Production Using Eucalyptus urograndis Hemicellulose Hydrolysate as a Carbon Source

Energies ◽  
2020 ◽  
Vol 13 (4) ◽  
pp. 795 ◽  
Author(s):  
Helberth Júnnior Santos Lopes ◽  
Nemailla Bonturi ◽  
Everson Alves Miranda
Keyword(s):  
Carbon Source ◽  
Lipid Production ◽  
Carbon Sources ◽  
Oil Production ◽  
Research Area ◽  
Microbial Oil ◽  
Hemicellulose Hydrolysate ◽  
Initial Ph ◽  
Different Temperatures ◽  
Active Research

Microbial oil is a potential substitute for vegetable oils in the biodiesel industry. Efforts to obtain cheap carbon sources for the cultivation of lipid-producing microorganisms comprise an active research area. This work aimed to extract the hemicellulose fraction from Eucalyptus uograndis and to use its hydrolysate as a carbon source for Rhodotorula toruloides (an oleaginous yeast) cultivation for microbial oil production. Hemicellulose hydrothermal extractions were performed at different temperatures, times, and ratios of solid to liquid (S/L). Temperature and time showed a stronger effect on the solubilization of hemicellulose. Hemicellulose extraction at 155 °C, 195 min, and an S/L ratio of 1/2 resulted in a hydrolysate with a xylose content of 37.0 g/l. R. toruloides cultivation in this hydrolysate showed that initial pH had a strong influence on cell growth. At an initial pH of 6.2, cells grew to 6.0 g/l of biomass with a lipid content of 50%. Therefore, we believe that E. urograndis hemicellulose hydrolysate could be a potential substrate for R. toruloides for lipid production based on the biorefinery concept.

The strain Gordonia alkanivorans 135 Is a Promising Destructor of Dibenzothiophene

2020 ◽  
Vol 36 (4) ◽  
pp. 121-125
Author(s):  
Е.Е. Frantsuzova ◽  
A.A. Vetrova
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Carbon Source ◽  
High Performance ◽  
Microbial Oil ◽  
Free Medium ◽  
Russian Science ◽  
Technological Method ◽  
Gordonia Alkanivorans

Genes involved in the dibenzothiophene degradation have been identified in the genome of Gordonia alkanivorans 135. The efficiency of the degradation was evaluated by high-performance liquid chromatography after the strain cultivation in mineral sulfur-free medium with glucose (hexadecane) as a carbon source at a temperature of 28 °C. The results obtained in this work allow us to consider the Gordonia alkanivorans 135 strain as promising for development of bio technological method for microbial oil desulfurization. Gordonia, dibenzothiophene, biodegradation. This work was financially supported by the Russian Science Foundation (Grant no. 19-74-00097).

Use of bacteriostatic antibiotics for the optimization of new media in view of supplementing POME as an alternative carbon source for PHA production - a statistical approach

2019 ◽  
Vol 16 ◽  
pp. 1692-1701
Author(s):  
Ponnaiah Paulraj ◽  
Harvie Anak Shukri ◽  
Vnootheni Nagiah ◽  
Nagaraja Suryadevara ◽  
Balavinayagamani Ganapathy
Keyword(s):  
Carbon Source ◽  
New Media ◽  
Statistical Approach ◽  
Alternative Carbon Source ◽  
Pha Production
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