scholarly journals Chemical composition, free-radical-scavenging and insecticidal activities of the aerial parts of Stachys byzantina

2010 ◽  
Vol 62 (3) ◽  
pp. 653-662 ◽  
Author(s):  
S. Asnaashari ◽  
A. Delazar ◽  
Seyed Alipour ◽  
L. Nahar ◽  
Angela Williams ◽  
...  
Keyword(s):  
High Pressure ◽  
Free Radical ◽  
Methanolic Extract ◽  
Solid Phase ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Spectroscopic Methods ◽  
Phase Extraction ◽  
Aerial Parts ◽  
The Family

Stachys byzantina K. Koch. is an Iranian endemic species of the genus Stachys L., which comprises about 300 species, and is one of the largest genera of the family Lamiaceae. A combination of solid phase extraction (SPE) and high pressure liquid chromatography (HPLC) of the methanolic extract of the aerial parts of S. byzantina afforded three phenylethanoids, 2'-O-arabinosyl verbascoside (1), verbascoside (2), aeschynanthoside C (3) and three flavones apigenin 7-O-glucoside (4), apigenin 7-O-(6-p-coumaroyl)-glucoside (5) and apigenin (6). The structures of these compounds were determined by spectroscopic methods. Free-radical-scavenging and insecticidal properties of the crude extracts, the fractions and the isolated compounds were assessed. .

scholarly journals Bioactivity of extracts of Centaurea polyclada dc. (Asteraceae)

2009 ◽  
Vol 61 (3) ◽  
pp. 447-452 ◽  
Author(s):  
Melanie Granger ◽  
Emilie Samson ◽  
Severine Sauvage ◽  
Anisha Majumdar ◽  
Poonam Nigam ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Free Radical ◽  
Solid Phase Extraction ◽  
Brine Shrimp ◽  
Solid Phase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phase Extraction ◽  
Brine Shrimp Lethality Assay

The free radical-scavenging property, antibacterial activity, and brine shrimp toxicity of n-hexane, dichloromethane (DCM), and methanol (MeOH) extracts of Centaurea polyclada, an endemic Turkish species, were assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, the resazurin microtiter plate-based assay, and the brine shrimp lethality assay, respectively. The DCM and MeOH extracts of C. polyclada exhibited free radical-scavenging ability with RC50 values 1.17 and 0.015 mg/mL, respectively. Among solid-phase extraction fractions of the MeOH extract, the fraction eluted with 60% MeOH in water demonstrated the highest level of free radical-scavenging activity (RC50 = 0.016 mg/mL). Only the DCM extract showed considerable antibacterial activity against all nine test strains except Escherichia coli, with MIC ranging from 1.25 to 2.50 mg/mL. This antibacterial activity pattern was also observed with solid-phase extraction fractions of the DCM extract with varied potencies. None of the extracts showed any significant toxicity towards brine shrimps (LD50 = >1.00 mg/mL).

scholarly journals Triterpenes from Warszewiczia coccinea (Rubiaceae) as Inhibitors of Acetylcholinesterase

2009 ◽  
Vol 4 (10) ◽  
pp. 1934578X0900401
Author(s):  
Angela I. Calderón ◽  
Johayra Simithy ◽  
Giuliana Quaggio ◽  
Alex Espinosa ◽  
Jose Luis López-Pérez ◽  
...  
Keyword(s):  
Free Radical ◽  
Thin Layer ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Spectroscopic Methods ◽  
Ache Inhibitors ◽  
Ache Inhibitory Activity ◽  
Stem Extract ◽  
Layer Chromatography ◽  
Free Radical Scavenging Activities

Seventy-six plant extracts from the Panamanian flora have been screened for acetylcholinesterase (AChE) inhibitors by thin-layer chromatography (TLC) bioautography. The most promising extracts with AChE inhibitory and free radical scavenging activities at 100 μg were those of Tabernaemontana panamensis (Markgr., Boiteau & L. Allorge) Leeuwenb., Pentagonia macrophylla Benth., and Warszewiczia coccinea (Vahl) Klotzsch. Bioguided fractionation of W. coccinea stem extract afforded two triterpenes, 3β,6β,19α-trihydroxy-urs-12-en-28-oic acid (1) and 3β,6β-dihydroxy-olean-12-en-28-oic acid (sumaresinolic acid) (2), with AChE inhibitory activity. Their structures were determined by spectroscopic methods. This is the first report of these bioactive triterpenes in W. coccinea.

scholarly journals Domestic Cooking Effects of Bambara Groundnuts and Common Beans in the Antioxidant Properties and Polyphenol Profiles

2017 ◽  
Vol 6 (2) ◽  
pp. 24 ◽  
Author(s):  
V. Nyau ◽  
S. Prakash ◽  
J. Rodrigues ◽  
J. Farrant
Keyword(s):  
Free Radical ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Esi Ms ◽  
Positive Effects ◽  
Phytochemical Profiles

Processing of legumes before consumption has several effects on micronutrients, macronutrients and phytonutrients. This study was undertaken to investigate the effect of domestic processing on antioxidant activities and phenolic phytochemicals of the red bambara groundnuts and red beans. The study employed in vitro antioxidant assays (DPPH and FRAP) to screen for antioxidant properties, HPLC-PDA-ESI-MS and Folin Ciocalteu assay to screen for phenolic phytochemical profiles. Domestic cooking displayed positive effects on the antioxidant activity and phenolic phytochemical profiles of the two legumes. The free radical scavenging speed increased 10-fold in the methanolic extract from cooked red bambara groundnuts compared to uncooked. By contrast, the free radical scavenging speed increased 20-fold in the methanolic extract from cooked red beans compared to uncooked. HPLC-PDA-ESI-MS profiles of the cooked red bambara groundnuts and red beans revealed a number of emergent phenolic compounds, mainly flavonoids. These data indicate that cooking appear to enhance the nutraceutical profiles of the legumes investigated.

Evaluation of the antioedematogenic, free radical scavenging and antimicrobial activities of aerial parts of Tillandsia streptocarpa Baker – Bromeliaceae

2004 ◽  
Vol 95 (2-3) ◽  
pp. 229-233 ◽  
Author(s):  
R.H. Delaporte ◽  
M.H. Sarragiotto ◽  
O.S. Takemura ◽  
G.M. Sánchez ◽  
B.P.D. Filho ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Free Radical Scavenging ◽  
Aerial Parts

scholarly journals Bioactivity of Centaurea persica boiss. (Asteraceae)

2012 ◽  
Vol 64 (2) ◽  
pp. 517-523 ◽  
Author(s):  
Satyajit Sarker ◽  
Lutfun Nahar ◽  
Srikanth Gujja ◽  
Shazi Begum ◽  
Sezgin Celik
Keyword(s):  
Antibacterial Activity ◽  
Free Radical ◽  
Solid Phase ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Disc Diffusion ◽  
Dpph Assay ◽  
Meoh Extract ◽  
E Coli ◽  
Diffusion Assay

The free-radical-scavenging property, antibacterial activity and brine shrimp toxicity of petroleum ether (PE), dichloromethane (DCM) and methanol (MeOH) extracts of Centaurea persica, a Turkish medicinal plant, were assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, the resazurin microtiter plate based assay, and the brine shrimp lethality assay, respectively. Additionally, the disc diffusion assay was also used to assess antibacterial activity. Only the MeOH extract of C. persica exhibited a significant free-radical-scavenging property in the DPPH assay, with an RC50 value of 0.025 mg/mL. However, in the TLC-based qualitative assay, petroleum ether and DCM extracts showed an extremely low level of free-radical-scavenging property. Among the solid-phase extraction fractions of the MeOH extract, the fractions eluted with 60% and 80% MeOH in water exhibited the highest level of free-radicalscavenging activity (RC50 = 0.010 and 0.015 mg/mL, respectively). While DCM extract showed reasonable antibacterial activity against five out of the nine test strains both in the disc diffusion assay and in the resazurin assay, the MeOH extract was highly active against both Escherichia coli and ampicillin-resistant E. coli strains. Among the solid-phase extraction fractions of the MeOH extract, fractions eluted with 80% MeOH in water and 100% MeOH displayed significant antibacterial potencies against both E. coli species. None of the extracts showed any significant toxicity towards brine shrimps (LD50 = >1.00 mg/mL).

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