scholarly journals The effect of lactic acid additive on the quality and chemical composition of meadow grass silage

2003 ◽  
Vol 19 (3-4) ◽  
pp. 61-69
Author(s):  
Jan Pyś ◽  
Wladislaw Migdal ◽  
Branislav Zivkovic ◽  
Olga Kosovac ◽  
Mihal Fabjan ◽  
...  

In the experiment, forage from the 1st cut of meadow grasses mown at the heading stage of dominant grasses was ensiled. The control silages were made from fresh forage (dry matter 223.5 g?kg-1) and wilted forage (dry matter 351.9 g?kg-1). The experimental silages were made from fresh forages supplemented with a 5% water solution of lactic acid (0.5 and 0.7 l?100 kg-1 of forage) and 8% water solution of lactic acid (0.4 and 0.6 l?100 kg-1 of forage). The highest contents of crude protein, water-soluble carbohydrates and energy were found in silages supplemented with 5% lactic acid applied at 0.7 l?100 kg-1 of forage and in silages supplemented with 8% lactic acid applied at 0.6 l?100 kg-1 of forage. The above silage variants were also characterized by the lowest content of NH3-N in total-N, butyric acid and acetic acid, as well as the highest contents of lactic acid and the best indicators of fermentation quality. No important differences were found between silages made from wilted forage and silages made with lactic acid supplement.

1996 ◽  
Vol 127 (4) ◽  
pp. 517-523 ◽  
Author(s):  
T. H. Aagnes ◽  
A. S. Blix ◽  
S. D. Mathiesen

SUMMARYReindeer calves(Rangifer tarandus tarandus) were used in parallel feeding trials with two different qualities of round-bale timothy silage in September 1992 (summer) and April 1993 (winter) in northern Norway, to determine the effect of season on their food intake and ability to utilize silage of different fibre contents. The silages were prepared from first-cut (FC) and regrowth (RG) timothy. The FC silage contained (on a dry matter (DM) basis) 12·3% crude protein, 30·4% cellulose and 6·2% water soluble carbohydrates (WSC), whereas the RG silage contained 14·3% crude protein, 18·7% cellulose and 30·0% WSC. For each trial, six male calves were taken directly from a natural range and divided into two groups of three animals. One group was offered FC silage and one RG silagead libitum, for 46 days. Mean daily DM food intake (g/kg liveweight) was not significantly different in summer compared to winter(P> 0·05), but significantly higher in the animals fed RG silage compared to the animals fed FC silage (P< 0·0001). The apparent digestibility coefficient of DM was high for both silages both in summer and winter, ranging from 74·4 to 86·6%. However, the digestibility in winter was significantly higher than in summer(P< 0·002) and the digestibility of RG silage was higher than that of the FC silage (P< 0·009). The ruminal VFA concentration was significantly higher in winter than in summer (P< 0·03) and in the animals fed RG silage (range 55·3–104·9 mM) compared to the animals fed FC silage (range 35·2–61·7 mM) (P< 0·002). In spite of the high digestibility of both the FC and RG silages, the nitrogen balance was significantly higher in the animals in winter compared to summer(P< 0·0001) and in the animals fed RG silage compared to the animals fed FC silage(P< 0·0001). Thus, utilization of grass silage in reindeer appears to be influenced both by season and by plant quality.


1981 ◽  
Vol 61 (3) ◽  
pp. 669-680 ◽  
Author(s):  
J. G. BUCHANAN-SMITH ◽  
Y. T. YAO

A silage additive containing lactic acid bacteria was tested for its effect upon preservation of corn silage, 35–40% dry matter (DM), in four pairs of upright silos (100-t capacity). The additive did not affect (P > 0.05) final pH, lactic or acetic acid concentration, crude protein or NPN-N and NH4+-N expressed as a percent of total N. Recovery of energy from treated silage was greater than control in the two pairs of silos where this was determined, but recoveries of dry matter and crude protein were not consistently affected. Two silage additives, containing hydrolytic enzymes and an antioxidant with or without lactic acid bacteria, were tested on alfalfa, 20, 30, 36.5 and 47.3% DM, in 250-mL laboratory silos. The experimental design used a4 (DM level) × 4 (added glucose — 0,4,8 and 12%, DM basis) × 3 (additive — control, a hydrolytic enzyme/antioxidant additive (E/AO), and E/AO plus lactic acid bacteria (E/AO +)) factorial arrangement of treatments. Alfalfa was harvested using farm equipment. For 60-day silage, the additives as either a primary factor in the design or in second-order interactions with either silage DM or glucose showed no effect (P > 0.05) upon pH, lactic acid, acetic acid, butyric acid, Fleig score, residual water-soluble carbohydrate and percent of total N as NPN-N or ammonia-N. Poor quality silage resulted from the fermentation of alfalfa at 20 and 30% DM, and although additional glucose resolved this problem the additives did not. Beneficial effects of additional water-soluble carbohydrate in alfalfa silage fermentation are evident in data presented. Thus an additive containing an antioxidant and hydrolytic enzymes was not very effective and numbers of lactic acid bacteria in untreated alfalfa harvested with farm equipment must already be sufficient for adequate fermentation.


1966 ◽  
Vol 66 (3) ◽  
pp. 351-357 ◽  
Author(s):  
W. Ellis Davies ◽  
G. ap Griffith ◽  
A. Ellington

The primary growth of eight varieties of three species–white clover (3), red clover (4) and lucerne (1)–was sampled at fortnightly intervals and the percentage dry matter, in vitro digestibility, crude protein, water soluble carbohydrates, P, Ca, K, Na and Mg were determined.Differences between species were nearly always significant and the general order of merit was white clover, red clover and lucerne. The exceptions were for dry-matter percentage where this order was reversed, and red clover had the lowest Na and highest Mg content.


Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1575
Author(s):  
Fuhou Li ◽  
Zitong Ding ◽  
Adegbola T. Adesogan ◽  
Wencan Ke ◽  
Yun Jiang ◽  
...  

The effects of two strains of class IIa bacteriocin-producing lactic acid bacteria, Lactobacillus delbrueckii F17 and Lactobacillus plantarum (BNCC 336943), or a non-bacteriocin Lactobacillus plantarum MTD/1 (NCIMB 40027), on fermentation quality, microbial counts, and aerobic stability of alfalfa silage were investigated. Alfalfa was harvested at the initial flowering stage, wilted to a dry matter concentration of approximately 32%, and chopped to 1 to 2 cm length. Chopped samples were treated with nothing (control, CON), Lactobacillus delbrueckii F17 (F17), Lactobacillus plantarum (BNCC 336943) (LPB), or Lactobacillus plantarum MTD/1 (NCIMB 40027) (LPN), each at an application rate of 1 × 106 colony-forming units/g of fresh weight. Each treatment was ensiled in quadruplicate in vacuum-sealed polyethylene bags packed with 500 g of fresh alfalfa per bag and ensiled at ambient temperature (25 ± 2 °C) for 3, 7, 14, 30, and 60 days. The samples were then subjected to an aerobic stability test after 60 days of ensiling. Compared with the CON silage, the inoculants reduced the pH after 14 days of ensiling. After 60 days, pH was lowest in the LPB-treated silage, followed by the F17 and LPN-treated silages. Inoculation of F17 increased concentrations of lactic acid in silages fermented for 7, 14, 30, and 60 days relative to other treatments, except for the LPN-treated silages ensiled for 30 and 60 days, in which the lactic acid concentrations were similar to that of F17 silage. Application of F17 and LPB decreased the number of yeast and mold relative to CON and LPN-treated silages. Compared with the CON silage, inoculant-treated silages had greater aerobic stability, water-soluble carbohydrate, and crude protein concentrations, and lower neutral detergent fiber, amino acid nitrogen, and ammonia nitrogen concentrations. The LPB-treated silage had the greatest aerobic stability followed by the F17-treated silage. Both class IIa bacteriocin producing inoculants improved alfalfa silage fermentation quality, reduced the growth of yeasts and molds, and improved the aerobic stability of the ensiled forage to a greater extent than the proven LPN inoculant. However, higher crude protein concentration and lower ammonia nitrogen concentration were observed in LPN-treated silage relative to other treatments.


1984 ◽  
Vol 64 (2) ◽  
pp. 411-425 ◽  
Author(s):  
G. W. MATHISON ◽  
L. P. MILLIGAN ◽  
J. WOHLLEBE ◽  
R. M. ELOFSON

Four experiments are described in which the effectiveness of SO2 as a forage treatment before ensiling was evaluated in terms of its influence on silage composition and nutritive value. Retention of added S to the time of feeding was 40% in one experiment and ranged from 17 to 32% in another experiment where SO2 was either applied in the field or at the silo. In general, more water-soluble carbohydrates were preserved in SO2-treated silage. Across all experiments the acetate concentration was reduced (P < 0.05) from 1.7 to 0.9% in the treated silage dry matter (DM). SO2 had no consistent effect on concentrations of other end products of microbial fermentation. In five experiments with legume or alfalfa-grsss silages the apparent digestibility of energy was increased (P < 0.05) by an average 2.6 percentage units whereas DM digestibility was not influenced (P > 0.05) by SO2 treatment. In contrast, energy (P < 0.05) and DM digestibilities were decreased when two cereal silages were treated with SO2. Cattle fed legume or legume-grass silage grew an average of 12.9% faster (P < 0.05) and exhibited a 12.7% improvement (P < 0.05) in DM conversion to liveweight gain in eight comparisons which have been made with SO2-treated forage to date. In contrast SO2 had no influence (P > 0.05) on the performance of steers in one 42-day trial with cereal silage. Further, SO2 was successfully applied in the field as well as at the silo and addition of a silage inoculant to SO2-treated forage was of no value. The use of SO2 as a preservative for legume and alfalfa-grass silage would be economically feasible under some circumstances but at this time treatment of cereal silages with the chemical cannot be recommended. Key words: Sulfur dioxide, silage, silage preservative, steers, nutritive value


2019 ◽  
Vol 28 (1) ◽  
Author(s):  
Marzenna Olszewska ◽  
Stefan Grzegorczyk ◽  
Anna Bałuch-Małecka

A field experiment was performed in 2011–2013. The aim of the study was to determine the effect of different proportions of Medicago media in mixtures with Festulolium braunii on the yield and feed value of green fodder, including the content of crude protein (CP) and water-soluble carbohydrates (WSC), and the carbohydrate-to-protein ratio. It was found that alfalfa had a high competitive advantage over Festulolium. In the second and third year of full utilization, alfalfa was the predominant species in the tested mixtures regardless of the proportion of sown seeds. The mixtures with alfalfa were characterized by high and stable dry matter yields and high protein content, whereas Festulolium significantly increased the content of WSC and improved the WSC:CP ratio. The evaluated mixtures had a higher feed value than pure-sown components. The results of the study indicate that Festulolium mixtures with a lower proportion (30% and 50%) of alfalfa are characterized by a higher feed value.


Animals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 253 ◽  
Author(s):  
M. Jordana Rivero ◽  
Oscar L. Balocchi ◽  
Fabián L. Neumann ◽  
Juan A. Siebald

The objective of this study was to evaluate the pasture performance of different cultivars of perennial ryegrass, two “high sugar” and two standard cultivars, under two contrasting agronomic managements (aimed at either decreasing or increasing water soluble carbohydrates concentration), and their effects on the grazing preference of dairy cows. Eight treatments arising from the factorial combination of four cultivars and two managements were randomly applied to 31-m2 plots in three blocks. Pasture dry matter production and growth rate were measured for one year. Three grazing assessments were performed to establish the grazing preferences of six dairy cows in spring, summer and autumn. High sugar cultivars produced less dry matter per hectare than the standard cultivars. Cows consumed more grass and harvested a greater proportion of the pasture under the agronomic management aimed at decreasing sugar concentration, i.e., with a greater nitrogen fertilization rate and under a more frequent defoliation regime, which could be explained by the greater crude protein concentration achieved under this management. The results suggest that the genetic selection for greater levels of sugars was at the expense of herbage yield, and that cows preferred to graze herbage with a greater crude protein level instead of a greater sugar concentration.


1982 ◽  
Vol 6 ◽  
pp. 187-188 ◽  
Author(s):  
A. R. Austin ◽  
M. Gill

The object of the experiment to be reported was to determine the extent to which intake and eating behaviour are influenced by differences in the ratio of nitrogen (N) to water soluble carbohydrates (WSC) in the feed and the liquid phase of boli obtained from oesophageal fistulae. Four feeds were chosen as being likely to cover a range of N to WSC ratios in the liquid phase; these were frozen lucerne, lucerne silage, grass silage and grass silage treated with formaldehyde (46 g/100 g crude protein).


2021 ◽  
Vol 9 (2) ◽  
pp. 420
Author(s):  
Hong Yang ◽  
Bing Wang ◽  
Qing Zhang ◽  
Hui Cheng ◽  
Zhu Yu

The use of the fermented total mixed ration (FTMR) is a promising approach for the preservation of homogeneous feed, but changes during fermentation and links with the bacterial community of FTMR are not fully understood. This study investigated the effects of adding oat silage (OS) to the fermented total mixed ration (FTMR) in terms of fermentation, chemical composition, and the bacterial community. The fermentation quality of FTMR with 22% OS was greatly improved, as demonstrated by decreases in the butyric acid concentration, a lower lactic acid/acetic acid ratio, a larger population of lactic acid bacteria (LAB), and quicker spoilage yeast death. Further examination of the effects of various ensiling days on nutritive values showed stable crude protein and nonprotein nitrogen (NPN) contents. The concentrations of acetic acid, propionic acid, and ammonia–nitrogen (NH3–N) were increased following all FTMR treatments after 15 d, while the concentration of water-soluble carbohydrates (WSC) was decreased. More heterofermentative LAB, such as Lentilactobacillus buchneri, Lentilactobacillus brevis, and Companilactobacillus versmoldensis were found after adding 11% and 22% OS. Moreover, the addition of 22% OS caused a marked increase in both bacterial richness and diversity, dominated by the Lactobacillus genus complex. Among species of the Lactobacillus genus complex, the occurrence of Loigolactobacillus coryniformis was positively correlated with lactic acid, NPN, and NH3–N concentrations, suggesting its potential role in altering the fermentation profiles.


2020 ◽  
Vol 33 (4) ◽  
pp. 556-567
Author(s):  
Zhumei Du ◽  
Na Risu ◽  
Ge Gentu ◽  
Yushan Jia ◽  
Yimin Cai

Objective: To improve the utility of native grass resources as feed in China, we investigated the dynamics of protein and carbohydrate fractions among Inner Mongolian native grasses, during ensiling and the aerobic stage, using the Cornell Net Carbohydrate and Protein System.Methods: Silages were prepared without or with lactic acid bacteria (LAB) inoculant. We analyzed the protein and carbohydrate fractions and fermentation quality of silages at 0, 5, 15, 20, 30, and 60 d of ensiling, and the stability at 0.5, 2, 5, and 10 d during the aerobic stage.Results: Inner Mongolian native grass contained 10.8% crude protein (CP) and 3.6% water-soluble carbohydrates (WSC) on a dry matter basis. During ensiling, pH and CP and WSC content decreased (p<0.05), whereas lactic acid and ammonia nitrogen (N) content increased (p<0.05). Non-protein N (PA) content increased significantly, whereas rapidly degraded true protein (PB<sub>1</sub>), intermediately degraded true protein (PB<sub>2</sub>), total carbohydrate (CHO), sugars (CA), starch (CB<sub>1</sub>), and degradable cell wall carbohydrate (CB<sub>2</sub>) content decreased during ensiling (p<0.05). At 30 d of ensiling, control and LAB-treated silages were well preserved and had lower pH (<4.2) and ammonia-N content (<0.4 g/kg of fresh matter [FM]) and higher lactic acid content (>1.0% of FM). During the aerobic stage, CP, extract ether, WSC, lactic acid, acetic acid, PB<sub>1</sub>, PB<sub>2</sub>, true protein degraded slowly (PB<sub>3</sub>), CHO, CA, CB<sub>1</sub>, and CB<sub>2</sub> content decreased significantly in all silages, whereas pH, ammonia-N, PA, and bound true protein (PC) content increased significantly.Conclusion: Control and LAB-treated silages produced similar results in terms of fermentation quality, aerobic stability, and protein and carbohydrate fractions. Inner Mongolian native grass produced good silage, nutrients were preserved during ensiling and protein and carbohydrate losses largely occurred during the aerobic stage.


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