scholarly journals Induction and micropropagation potential of sugar beet haploids

Genetika ◽  
2004 ◽  
Vol 36 (3) ◽  
pp. 187-194 ◽  
Author(s):  
Nevena Nagl ◽  
Snezana Mezei ◽  
Lazar Kovacev ◽  
Dragana Vasic ◽  
Nikola Cacic
Keyword(s):  
Sugar Beet ◽  
Correlation Coefficients ◽  
Ovule Culture ◽  
Axillary Buds ◽  
Haploid Plant ◽  
Multiplication Rate ◽  
Adventitious Buds ◽  
Nutrient Media

The aim of research was obtaining sugar beet haploids via gyno-genesis and their micropropagation. Haploids were obtained by ovule culture from fourteen diploid, monogerm, fertile genotypes. On the tested nutrient media genotypes exhibited different gynogenic potential. Eight haploid plant were chosen for further investigation and after development of first leaves put on micropropagation medium. The presence of cyto-kinin in medium stimulated development of axillary buds, while in some genotypes adventitious buds developed as well. Multiplication rate was not consistent, although number of developed plants grew after each sub-cultivation. Differences in plant multiplication started to differ after four subcultures. By testing of differences between correlation coefficients, i.e. multiplication rate during six subcultivations, it was determined that they significantly differ between tested genotypes.

Experiments on the spacing of sugar beet: I. Results based on plot yields

1939 ◽  
Vol 29 (1) ◽  
pp. 48-57 ◽  
Author(s):  
F. H. Garner ◽  
H. G. Sanders
Keyword(s):  
Sugar Beet ◽  
Compensatory Growth ◽  
Correlation Coefficients ◽  
General Rule ◽  
The West ◽  
Spacing Factor ◽  
West Midlands ◽  
Series Of Experiments ◽  
Effect On Yield ◽  
The Relationship

Since sugar beet was first introduced into this country many experiments have been conducted to determine the effects of spacing on yield: the general advice based on these experiments is that beet should be spaced as closely as working conditions permit. Davies (1931) carried out a series of experiments in the West Midlands, and came to the conclusion that yield was not related to the number of roots per acre, but was affected by their distribution. His work demonstrated that wide row distances could not be compensated by narrow spacing in the row. He found that yield increased as row distance decreased down to 16 in., but that singling distances of 4—10 in. produced no differences in yield of roots: the yield of green leaves, on the other hand, was increased as singling distance decreased, but was unaffected by row distance (Davies & Dudley, 1929). Although Davies' results would be generally accepted as a true expression of the general rule, many isolated spacing experiments fail to conform to them; it is possible that discrepancies in results, that undoubtedly occur, may be due to variations in the “plant” actually obtained in the experiments. Engledow et al. (1928), as a result of counts and weights taken on ordinary farm crops of sugar beet, concluded that uniformity of “plant” was a most important spacing factor affecting yield. In America Brewbaker & Deming (1935) have found yield to be related to percentage stand (correlation coefficients varying from +0·35 to +0·70), the regression between the two variables being approximately linear over the range studied. They also found that uniformity of “plant” was more important than spacing distances, either between or in the rows. Their work showed that single gaps had little effect on yield, because neighbouring beet compensated for them to the extent of 96·2%; serious loss of yield only occurred, therefore, with adjacent gaps. Pedersen (1933) studied the relationship between percentage of gaps and yield in a large number of Danish experiments with sugar beet and mangolds. In the case of sugar beet he found that the compensatory growth of neighbouring roots amounted to 76% for a single gap, and that the percentage compensation decreased as the size of gap (i.e. number of missing beet) increased. In an earlier paper (Pedersen, 1931) he had shown that under ordinary field conditions the distribution of gaps was approximately random.

scholarly journals Effect of Winter Oilseed Rape Cropping on the Development of the Sugar Beet Cyst Nematode, Heterodera schachtii, and Control of Volunteer Plants as a Trap Crop Method

Agronomy ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 355
Author(s):  
Daub Matthias
Keyword(s):  
Sugar Beet ◽  
Oilseed Rape ◽  
Cyst Nematode ◽  
Heterodera Schachtii ◽  
Population Increase ◽  
Management Tool ◽  
Multiplication Rate ◽  
Degree Days ◽  
Trap Crop ◽  
Beet Cyst Nematode

The integration of oilseed rape (OSR) into sugar beet rotation systems is restricted due to the very good host status of OSR for the beet cyst nematode (BCN) Heterodera schachtii. In contrast to sugar beet, the cultivation of winter OSR covers a longer period, but at a lower soil temperature regime. Thus, presumably one or two generations of BCN may develop during the cultivation of winter OSR, resulting in moderate multiplication rates of 1–2 in the present study. This multiplication rate was year-dependent, but not affected by different sowing times. For the first time, the present study identified volunteer OSR emerging in high densities post-harvest as a major risk for a high multiplication of BCN at optimum temperatures. The emergence of BCN females with offspring was observed very early, resulting in a significant population increase before 350-degree days (>8 °C) in inoculation experiments and in field investigations. Conducting treatment trials with glyphosate to control volunteer OSR in micro-plots and field experiments confirmed effective suppression of BCN reproduction when growth of volunteer OSR was interrupted at 250–350-degree days. Thus, data gained from BCN reproduction studies under controlled and field conditions provided a unique basis for the development of a trap crop method. The degree day model has been successfully implemented as part of an open access management tool.

scholarly journals Modern issues of sugar beet (Beta vulgaris L.) hybrid breeding

2021 ◽  
Vol 25 (4) ◽  
pp. 394-400
Author(s):  
S. D. Karakotov ◽  
I. V. Apasov ◽  
A. A. Nalbandyan ◽  
E. N. Vasilchenko ◽  
T. P. Fedulova
Keyword(s):  
Mitochondrial Genome ◽  
Sugar Beet ◽  
High Performance ◽  
High Efficiency ◽  
Practical Importance ◽  
Hybrid Breeding ◽  
Haploid Plant ◽  
Male Sterile ◽  
Rapd Primer

High efficiency of the cultivation of unfertilized sugar beet ovules and preparation of haploid regenerants (microclones) of pollinators – maintainers of О-type sterility and MS forms of the RMS 120 hybrid components has been shown. A technological method that accelerates the creation of new uniform starting material is proposed. It speeds up the breeding process two to threefold. The identification of haploid regenerants with sterile cytoplasm in initial populations is of great theoretical and practical importance for breeding, as it facilitates the production of homozygous lines with cytoplasmic male sterility and high-performance hybrids on sterile basis. As shown by molecular analysis, a single-nucleotide polymorphism never reported hitherto is present in the mitochondrial genome of the haploid plant regenerants. It allows identification of microclones as fertile and sterile forms. It has been found that DNA markers of the sugar beet mitochondrial genome belonging to the TR minisatellite family (TR1 and TR3) enable reliable enough identification of haploid microclonal plants as MSor O-type forms. Fragments of 1000 bp in length have been detected in monogenic forms in the analysis of 11 sugar beet plants cultured in vitro by PCR with the OP-S4 random RAPD primer. Testing of the OP-S4 marker’s being in the same linkage group as the genes responsible for expression of the economically valuable trait monogermity demonstrates its relative reliability. By the proposed method, dihaploid lines (DH) of the male-sterile form and the О-type sterility maintainer of the RMS 120 sugar beet hybrid have been obtained in in vitro culture. These lines are highly uniform in biomorphological traits, as proven under field conditions.

scholarly journals Perbanyakan tanaman kina Cinchona ledgeriana Moens. dan C. succirubra Pavon melalui penggandaan tunas aksiler Propagation of cinchona plant Cinchona ledgeriana Moens. and C. succirubra Pavon through axillary buds multiplication

2016 ◽  
Vol 72 (1) ◽  
Author(s):  
. JOKO-SANTOSO ◽  
Nurita TORUAN-MATHIUS ◽  
U SASTRAPRAWIRA ◽  
G SURYATMANA ◽  
D SAODAH
Keyword(s):  
Tissue Culture ◽  
Rice Husk ◽  
Shoot Multiplication ◽  
Culture Technique ◽  
Axillary Buds ◽  
Multiplication Rate ◽  
Plant Materials ◽  
Tissue Culture Technique ◽  
Cinchona Ledgeriana

SummaryCinchona ledgeriana (Ledger) and C. succirubra (Succi) were industrial commodities which their barks of the trunk  contain alkaloid   used as raw materials in pharmaceutical, food, drug and beverages and chemical industries. The problem  faced in conventional plant propagation are. incompatibilities, high numbers of death caused by transportation, limited numbers and time consume in  plant materials  production. These problems may be  overcome by axillary buds multiplication.  The aim of the experiment were to find out propagation technology of Ledger and Succi by  tissue culture technique.  Experiments were conducted in three consecutive steps, viz the effect of (i) BAP on multiplication and growth of axillary’s bud of Ledger and Succi in vitro culture, (ii) IBA on root initiation and growth, (iii)  growth medium on the growth of plantlets in  acclimatization.The design of the experiments were Complete Randomized Design with 15 (i & ii) and four (iii) replications. The treatments were (i) 0,1,2,3,4, dan 5 mg/L BAP, (ii) 0.0; 0.5; 1.0; 1.5; 2.0; dan 2.5 mg/L IBA, and (iii) mixture of soil and rice husk charcoal (1:1), mixture of soil and compost (1:1),  mixture of soil, rice husk charcoal, and  compost  (1:1:1).  Parameters measured in the experiments were (i) the initiation of buds multiplication rate twice at axillary buds at subculture.  (ii) initiation  and  roots vigor. (iii) numbers of survived  plants and plants vigor. The explant source used derived from two-month old axillary buds cultured in Murashige and Skoog (MS) medium without growth regulator. Results of the experiment showed  that the best shoot multiplication of Ledger  and Succi  was obtained from the application of 3 mg/L BAP, with buds multipli-cation rate 7 buds/explant/month for Ledger, and 3-4 buds/explants/month for succi. The best root initation and root growth were found from the application of 2 mg/L IBA. The highest percentage of survived plantlets (100%) in acclimatization was obtained from mixture of soil and rice husk charcoal (1:1) medium.  Therefore it is  concluded that tissue culture technique could be used for planlet  mass propagation    of  elite C. Ledgeriana and C. Succirubra through axillary bud multiplication.Ringkasan Tanaman kina Cinchona ledgeriana (Ledger) dan C. succirubra (Succi)  merupakan tanaman industri yang mengandung alkaloid di dalam kulit batangnya dan berguna dalam bidang industri farmasi, makanan, minuman dan kimia. Kendala yang dihadapi dalam perbanyakan tanaman kina secara konvensional dengan sistem sambung  adalah inkompatibilitas,    kematian akibat pengangkutan cukup tinggi, jumlah bahan tanam yang diproduksi sangat terbatas dan waktu penyediaan yang cukup lama. Masalah tersebut dapat diatasi dengan menggunakan teknik kultur jaringan. Penelitian ini bertujuan untuk men-dapatkan teknologi perbanyakan tanaman kina Ledger dan Succi dengan teknik kultur jaringan. Penelitian terdiri atas (i) pengaruh BAP terhadap inisiasi dan penggandaan  tunas aksilar, (ii) pengaruh IBA terhadap inisiasi serta pertum-buhan akar planlet,   dan (iii) pengaruh beberapa medium terhadap pertumbuhan planlet dalam aklimatisasi. Percobaan menggunakan Rancangan Acak Lengkap, masing-masing diulang 15 (i & ii)  dan (iii) empat kali. Peubah yang diukur untuk percobaan (i) adalah waktu inisiasi tunas dan laju penggandaan tunas aksiler pada dua  kali  subkulur. (ii)  Waktu  inisiasi  dan vigor akar. (iii) Jumlah tanaman yang bertahan hidup setelah aklimatisasi, serta vigor tanaman. Sumber eksplan yang digunakan adalah tunas aksilar dari kecambah terpilih berumur dua bulan yang dikulturkan dalam medium Murashige dan Skoog tanpa zat pengatur tumbuh. Perlakuan untuk percobaan (i) adalah 0,0; 1,0; 2,0; 3,0; 4,0 dan  5,0 mg/L BAP, (ii) adalah 0,0; 0,5; 1,0; 1,5; 2,0; dan 2,5 mg/L IBA, sedang (iii) adalah medium tanam tanah, tanah : arang sekam (1:1),  tanah : kompos (1:1), tanah : arang sekam : kompos (1:1:1). Hasil yang diperoleh menunjukkan bahwa konsentrasi BAP terbaik untuk inisiasi dan penggandaan tunas tanaman kina Ledger dan Succi adalah 3 mg/L BAP, dengan laju penggandaan tujuh tunas/eksplan/bulan untuk Ledger dan 3-4 tunas/eksplan/bulan untuk Succi. Sedang untuk perakaran diperoleh dari medium MS dengan penambahan 2 mg/L IBA. Persentase tertinggi planlet (100%) yang mampu bertahan hidup pada aklimatisasi diperoleh dari medium campuran tanah : arang sekam (1:1). Berdasarkan hasil tersebut di atas dapat disimpulkan bahwa perbanyakan tanaman kina secara in vitro untuk menghasilkan bibit bermutu dapat dilakukan melalui teknik penggandaan tunas aksiler

The assessment of the quality of sugar-beet seed

1985 ◽  
Vol 104 (1) ◽  
pp. 71-84 ◽  
Author(s):  
M. J. Durrant ◽  
S. J. Brown ◽  
A. Bould
Keyword(s):  
Sugar Beet ◽  
Field Experiments ◽  
Correlation Coefficients ◽  
Germination Percentage ◽  
Controlled Conditions ◽  
High Viability ◽  
Rate Of Emergence ◽  
Average Standard Error

SummaryThe accurate prediction of the performance of individual lots of sugar-beet seed in the field is critical when constituting blends of seed for commercial use. Previous studies indicated a need for ‘vigour tests’ to supplement the standard germination test. In many of the studies emphasis was placed on evaluation of tests in the laboratory, whereas in this study equal emphasis was placed on the determination of the values to be predicted. The performance of 20 seed lots with a range of germination between 75 and 95% was assessed in field and laboratory studies in 1981. Further investigations of ten seed lots were made in 1982 and in 1983.The results in 1981 showed little evidence of agronomically important differences in the relative performance of seed lots with respect to establishment in the field at different sites. Similarly, most seed lots showed consistent differences over a range of tests under controlled conditions. However, use of the experiment mean as a measure of stress revealed marked differences in the range of performance between the best and poorest lota in both the field and laboratory. There were clear indications that performance in the field could be modified by non-random factors that tended to reduce the magnitude of differences among seed lots. In 1982 grazing of seedlings by birds was identified as a factor that could cause greater reduction in the establishment of seed lots with high viability relative to those with low viability. Thus, under some circumstances, a single count of seedlings in non-protected field experiments can give misleading estimates of the differences among seed lots (and probably among other treatments which affect the rate of emergence). Correlation coefficients (γ) were about 0·88 for relationships between establishment from pelleted seed in plots protected from birds and germination or emergence under controlled conditions. These results reduced the need for additional vigour tests. However, the studies emphasized that small differences in germination percentage are important as they can be associated with much larger differences in establishment, particularly under adverse seed-bed conditions. The average standard error for the mean viability of individual seed lots was ±1·89 in germination tests with four replicates of 100 seeds at each of six centres. Although inincreased accuracy may not be possible without much greater replication, we found that the distinction between normal and abnormal seedlings was an important source of experimental error. A less subjective measurement, based on hypocotyl height, warrants further study.

A refined method for ovule culture in sugar beet (Beta vulgaris L.)

2021 ◽  
Author(s):  
Sara Sohrabi ◽  
Mohammad Reza Abdollahi ◽  
Asghar Mirzaie-Asl ◽  
Hassan Ebrahimi Koulaei ◽  
Mohsen Aghaeezadeh ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Ovule Culture ◽  
Refined Method

scholarly journals In vitro rhizogenesis of sugar beet microclones

2017 ◽  
Vol 8 (4) ◽  
pp. 616-622
Author(s):  
V. V. Polishchuk ◽  
L. M. Karpuk ◽  
V. P. Mykolaiko ◽  
A. A. Polishchuk ◽  
I. I. Mykolaiko
Keyword(s):  
Sugar Beet ◽  
Chromosomal Abnormalities ◽  
Morphological Changes ◽  
Lateral Roots ◽  
Morphological Characteristics ◽  
In Vitro Cultivation ◽  
Male Sterile ◽  
Nutrient Media ◽  
Lower Efficiency

The features of rhizogenesis of male-sterile simple hybrids, maternal components of sugar beet heterozosis hybrid and O-types of Darinka variety grown from seeds were investigated. The paper presents the results of studying the main basic nutrient media (B5–A1, B5–A2, B5–A3, B5–A4, B5–A5 and B5–A6) for rhizogenesis, in which the content of macro- and microelements has been reduced by three times, the content of sucrose is reduced by almost 6 times, the amount of agar is reduced by almost five times, while the vitamin РР content is increased by 1.6 times, and the BAP is completely removed. The largest number of rooted microclones was obtained with NAAs by the high indexes of average number of roots and medium indexes of average roots length. To the second, by the number of rooted microclones is assigned, media of 2,4–D, 2,4–DB containing, potassium salt of NAA and IBA were classified, while IAA was characterized by a lower efficiency. The possibilities of regulation of growth and development processes of sugar beet in vitro explants in favor of undifferentiated growth at the stage of proliferation or organogenesis (hemo- and rhizogenesis) have been determined. To improve the quality of rooting of sugar beet plants-regenerants, we have developed the method for dimming agarized nutrient medium with methylene blue at a 0.05% concentration, which made it possible to reduce the inhibitory effect of light on the development of lateral roots. The average index of micro-roots rooting for MS-forms was 81.5 %. The technology of adaptation of micro-roots rooting was developed, in which the plant material at the initial stage of growth had slight morphological changes in the puffer apparatus and stem, but by the end of the vegetation, the plants acquired the appearance characteristic of the donor plants of explants. The clone’s resiliency is reached about 100% in the soil. According to the variability of morphological characteristics in reproduced in vitro sugar beet plants of the MS-forms of the Darynka hybrid, all the plants grown from the regenerants were more, than seed progeny from seed sowing of the same component obtained as a result of the attachment of sterility to the corresponding O-type. It has been found that in vitro cultivation has not weakened, and in some cases even increased the mitotic index of meristem cells of young roots of sugar beet. Most genotypes have reduced the number of pathological mitoses, apparently due to the activation of reparative systems, but did not extend the duration of individual phases of mitosis; without increasing the number of anaphase with bridges and fragments, but changed the number of anaphases with other chromosomal abnormalities. In all variants of the experiment, where activation of growth processes has been observed, the lowest variability of the signs is observed, that is, under the influence of stimulants, which are part of nutrient media, obviously there is unification of cell populations. 

Sign in / Sign up

Export Citation Format

Share Document