Effect of Low Atmospheric Pressure on Bubble Stability of Air-Entrained Concrete

The effect of low atmospheric pressure of the environment on the air content and bubble stability of air-entrained concrete was investigated in Beijing and Lhasa. The results indicate that the reduction of atmospheric pressure can weaken the air-entraining capability of air-entraining agents (AEAs). The air content of fresh concrete decreased by 9%–39% when the atmospheric pressure dropped to 64 kPa. The bubble stability of concrete mixed at a low atmospheric pressure becomes worse. Within 50–55 min after mixing, the air content of concrete mixed at a low atmospheric pressure decreases greatly, and the void spacing factor increases obviously. The concrete mixed at a low atmospheric pressure will lose more air content when vibration time increases, leading to the decrease of air content and the increase of the spacing factor, which are more significant than the concrete mixed at normal atmospheric pressure. On the basis of the experiment results in this study, the type of AEAs must be carefully selected, and the vibration time must be strictly controlled to ensure that the air content of concrete will meet the design requirements in low atmospheric pressure areas.

RSF1 in cancer: interactions and functions

RSF1, remodelling and spacing factor 1, is an important interphase centromere protein and is overexpressed in many types of cancers and correlated with poor overall survival. RSF1 has functions mainly in maintaining chromosome stability, facilitating DNA repair, maintaining the protein homeostasis of RSF1 and suppressing the transcription of some oncogenes when RSF1 protein is expressed at an optimal level; however, RSF1 overexpression facilitates drug resistance and cell cycle checkpoint inhibition to prompt cancer proliferation and survival. The RSF1 expression level and gene background are crucial for RSF1 functions, which may explain why RSF1 has different functions in different cancer types. This review summarizes the functional domains of RSF1, the overexpression status of RSF1 and SNF2H in cancer based on the TCGA and GTEX databases, the cancer-related functions of RSF1 in interacting with H2Aub, HDAC1, CENP-A, PLK1, ATM, CENP-S, SNF2H, HBX, BubR1, cyclin E1, CBP and NF-κB and the potential clinical value of RSF1, which will lay a theoretical foundation for the structural biology study of RSF1 and application of RSF1 inhibitors, truncated RSF1 proteins and SNF2H inhibitors in the treatment of RSF1-overexpressing tumours.

Maleic Rosin-based Gemini Surfactant as Air Entraining Agent for Concrete under Low Air Pressure

The effectiveness of Air entraining agent (AEA) in concrete under low air pressure in the plateau area decreased. A type of new AEA, named MRE was synthesized to increase bubbles` stability in fresh concrete under low air pressure. The performance of MRE solution and concrete with MRE were tested under 60 kPa and 100 kPa compared with commercially gemini AEA (DCC). The test results showed that the foam volume of MRE and DCC solution under 60 kPa was reduced by 3% and 9% than under 100 kPa. The bubble liquid film strength of MRE is 63% higher than that of DCC. For concrete with MRE and DCC under 60 kPa, the air content was 2% and 16% lower, the relative dynamic modulus of concrete reduced by 6% and 15%, and the bubble spacing factor under 60 kPa increased by 17% and 39% respectively compared with that under 100 kPa. MRE can increase the freeze-thaw resistance of concrete under low air pressure without affecting concrete strength and is suitable for high altitude concrete.

Basis of specificity for a conserved and promiscuous chromatin remodeling protein

Eukaryotic genomes are organized dynamically through the repositioning of nucleosomes. Isw2 is an enzyme that has been previously defined as a genome-wide, non-specific nucleosome spacing factor. Here, we show that Isw2 instead acts as an obligately targeted nucleosome remodeler in vivo through physical interactions with sequence-specific factors. We demonstrate that Isw2- recruiting factors use small and previously uncharacterized epitopes, which direct Isw2 activity through highly conserved acidic residues in the Isw2 accessory protein Itc1. This interaction orients Isw2 on target nucleosomes, allowing for precise nucleosome positioning at targeted loci. Finally, we show that these critical acidic residues have been lost in the Drosophila lineage, potentially explaining the inconsistently characterized function of Isw2-like proteins. Altogether, these data suggest an 'interacting barrier model' where Isw2 interacts with a sequence-specific factor to accurately and reproducibly position a single, targeted nucleosome to define the precise border of phased chromatin arrays.

Basis of Specificity for a Conserved and Promiscuous Chromatin Remodeling Protein

Eukaryotic genomes are organized dynamically through the repositioning of nucleosomes. Isw2 is an enzyme that has been previously defined as a genome-wide, non-specific nucleosome spacing factor. Here, we show that Isw2 instead acts as an obligately targeted nucleosome remodeler in vivo through physical interactions with sequence-specific factors. We demonstrate that Isw2-recruiting factors use small and previously uncharacterized epitopes, which direct Isw2 activity through highly conserved acidic residues in the Isw2 accessory protein Itc1. This interaction orients Isw2 on target nucleosomes, allowing for precise nucleosome positioning at targeted loci. Finally, we show that these critical acidic residues have been lost in the Drosophila lineage, potentially explaining the inconsistently characterized function of Isw2-like proteins. Altogether, these data suggest an “interacting barrier model” where Isw2 interacts with a sequence-specific factor to accurately and reproducibly position a single, targeted nucleosome to define the precise border of phased chromatin arrays.

Effects of Parameters of Air-Avid Structure on the Salt-Frost Durability of Hardened Concrete

Through laboratory testing, this research studied the connection between air-void structures of hardened concrete and fresh concrete and discussed the effects of the air-void structure on the salt-frost durability of the concrete. The results demonstrate that, in comparison with fresh concrete, the air-void spacing factor shows a close correlation with hardened concrete air-content and decreases in the form of a power function as the air-content increases. When the fresh concrete air-content is more than 6% and the hardened concrete air-void spacing factor is less than 0.18 mm, the influence of parameters of air-void structure on the salt-frost resistance of the concrete reduces. The air-void spacing factor more significantly affects the salt-frost resistance of the concrete compared with air content and the correlation reaches 0.93. Therefore, air-content and air-void spacing factor are recommended for dual control.

Additional Porosity as a Side Effect of Polycarboxylate Addition and Its Influence on Concrete’s Scaling Resistance

A side effect of using modified polycarboxylates to liquefy a concrete mix is additional pores in the concrete. They change the air void system in hardened concretes, and can be used to evaluate the freeze–thaw resistance of concretes. The purpose of this study is to determine the impact of the abovementioned quantitative and qualitative parameters on the freeze–thaw resistance of concretes. The research program was performed on eight sets of air-entraining and non-air-entraining concretes with a variable content of superplasticizer based on modified polycarboxylates. The basic composition of and air-entraining admixture content in the air-entraining concrete mixtures were held constant. Pore structure tests were performed according to EN 480-11. Scaling resistance was determined according to PKN-CEN/TS 12390-9. The results showed that as the content of modified polycarboxylates increased, the pore structure was adversely affected, and, consequently, the air void parameters deteriorated. At the same time, the freeze–thaw resistance of the non-air-entraining concretes decreased. The pores sizes also changed. As the fluidity increased, the specific surface area decreased, and, consequently, the spacing factor increased. The air-entraining concretes, despite the deterioration in the pore structure due to the modified polycarboxylates, were found to be very good quality concretes after 56 freeze–thaw cycles in the presence of 3% NaCl.

Comparative Evaluation on Performance Characteristics of an Impact Sprinkler with Nozzle-Dispersion Devicesand Rotary Plate Sprinkler

A set of nozzle and water dispersion devices were designed to obtain a desirable jet breakup and save water from an impact sprinkler (IS) at low pressure conditions. Comparative performance indicators were obtained for the commercial LP and IS sprinkler heads. Results show that under the same working conditions, the IS produced a large spray range and the water distribution in the middle of the spray range (R) was better than the LP. The best simulated uniformity from the IS was obtained for the combined spacing factor of 1.4*R and the combined uniformity was 4% larger than the LP. Considering the middle position of the spray range, the number and diameter of the droplets from the IS were larger than the droplets from the LP. However, in the end position of the spray range, the droplet diameters from the IS were quite smaller than the LP. Velocities from IS ranged between 0.2 and 6.4 m/s, while that from LP sprinkler were slightly higher ranging from 0.15 to 7.7 m/s at 150 kPa. Overall, introduction of the fixed dispersion device with a pointed tip profile combined with the 6 mm nozzle offered some advantages for the IS over the LP sprinkler at low-pressure conditions. Keywords: Dispersion device, Droplet distribution, Impact sprinkler, Low pressure sprinkler, Nozzle, Spray range, Uniformity.