Growth and photosynthetic traits differ between shoots originated from axillary buds or from adventitious buds in Populus balsamifera L. cuttings

2021 ◽  
Author(s):  
Lei Wang ◽  
Qing‐Lai Dang
Keyword(s):  
Axillary Buds ◽  
Adventitious Buds ◽  
Populus Balsamifera ◽  
Photosynthetic Traits

scholarly journals Induction and micropropagation potential of sugar beet haploids

Genetika ◽  
2004 ◽  
Vol 36 (3) ◽  
pp. 187-194 ◽  
Author(s):  
Nevena Nagl ◽  
Snezana Mezei ◽  
Lazar Kovacev ◽  
Dragana Vasic ◽  
Nikola Cacic
Keyword(s):  
Sugar Beet ◽  
Correlation Coefficients ◽  
Ovule Culture ◽  
Axillary Buds ◽  
Haploid Plant ◽  
Multiplication Rate ◽  
Adventitious Buds ◽  
Nutrient Media

The aim of research was obtaining sugar beet haploids via gyno-genesis and their micropropagation. Haploids were obtained by ovule culture from fourteen diploid, monogerm, fertile genotypes. On the tested nutrient media genotypes exhibited different gynogenic potential. Eight haploid plant were chosen for further investigation and after development of first leaves put on micropropagation medium. The presence of cyto-kinin in medium stimulated development of axillary buds, while in some genotypes adventitious buds developed as well. Multiplication rate was not consistent, although number of developed plants grew after each sub-cultivation. Differences in plant multiplication started to differ after four subcultures. By testing of differences between correlation coefficients, i.e. multiplication rate during six subcultivations, it was determined that they significantly differ between tested genotypes.

In vitro regeneration of plantlets of Scots pine (Pinus sylvestris) with mycorrhizal roots from subcultured callus initiated from needle adventitious buds

1994 ◽  
Vol 72 (8) ◽  
pp. 1144-1150 ◽  
Author(s):  
Supriyanto ◽  
R. Rohr
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
In Vitro Regeneration ◽  
Histological Study ◽  
Axillary Buds ◽  
Adventitious Buds ◽  
Meristematic Tissue ◽  
Hebeloma Cylindrosporum ◽  
Half Strength

Plantlets were regenerated from cultures established from Pinus sylvestris (Scots pine) meristematic tissue. Seedling explants were first stimulated to develop axillary buds. Developing axillary buds produced numerous new meristems that gave rise to globular adventitious buds located along the needles on half-strength modified Murashige and Skoog medium supplemented with coconut milk and 6-benzylaminopurine. A histological study showed that these new buds originated from dedifferentiated mesophyll and epidermal tissues of the needles. Some of these buds were used for the regeneration of whole plantlets, others were excised and transferred to woody plant medium, on which calli developed at the bases of the microcuttings These calli were organogenic when subcultured on a hormone-free medium and initiated a large number of rooted plantlets that showed high potential to multiply themselves indefinitely. This is the first report of regeneration of Scots pine from a subculturable organogenic line. Mycorrhizae were initiated for both types of plantlets with Hebeloma cylindrosporum on a perlite substrate under fully controlled conditions. Mycorrhizae improved the transfer of the plantlets to ex vitro conditions. Key words: organogenesis, mycorrhizae, tissue culture, Pinus sylvestris.

Reiteration in the short lived root-sprouting herb Rorippa palustris: does the origin of buds matter?

Botany ◽  
2010 ◽  
Vol 88 (7) ◽  
pp. 630-638 ◽  
Author(s):  
Alena Bartušková ◽  
Jitka Klimešová
Keyword(s):  
Aboveground Biomass ◽  
Adventitious Shoots ◽  
Fruit Production ◽  
Axillary Buds ◽  
Adventitious Buds ◽  
Extrinsic Factors ◽  
Axillary Shoots ◽  
Stem Base ◽  
Intrinsic Factors ◽  
Architectural Characteristics

Regrowth of injured plants occurs from dormant meristems. Its success is known to be dependent on extrinsic factors, such as the severity of an injury and nutrient availability, although little is known about the effect of intrinsic factors. We conducted a growth-chamber experiment with the short-lived root-sprouting herb Rorippa palustris (L.) Besser, where we evaluated the role of bud origin on plant regrowth after disturbance. In particular, we investigated whether shoots sprouting from either axillary or adventitious buds differ in compensatory growth and architecture. We triggered resprouting from axillary versus adventitious buds by two levels of disturbance: (i) removal of the whole aboveground biomass, with axillary buds on the stem base and adventitious buds on roots left intact; and (ii) removal of whole aboveground biomass including axillary buds on the stem base, and only adventitious buds left intact. Despite the fact that the adventitious buds were larger and contained more leaf primordia than axillary buds at the time of disturbance, the latter preferentially resprouted when both types of buds were present. Both axillary and adventitious shoots compensated for lost aboveground biomass; nevertheless, only axillary shoots compensated for fruit production. Shoot reiteration after injury was only partial in both types of shoot; however, adventitious shoots in differed more in their architectural characteristics than axillary shoots did. We confirmed that the output of plant regeneration after disturbance depends also on intrinsic factors such as the origin of the buds.

Development of supernumerary buds from the axillary meristem of pea, Pisum sativum (Fabaceae)

2000 ◽  
Vol 48 (2) ◽  
pp. 271 ◽  
Author(s):  
Joel P. Stafstrom ◽  
Vinita B. Sarup
Keyword(s):  
Pisum Sativum ◽  
Apical Dominance ◽  
Higher Plants ◽  
External Morphology ◽  
Axillary Buds ◽  
Leaf Axil ◽  
Adventitious Buds ◽  
Axillary Meristem ◽  
Pisum Sativum L

Leaf axils of higher plants commonly contain vegetative axillary buds, which are derived from an axillary meristem. The persistence and continued organogenic activity of the axillary meristem has been studied experimentally in only two species. Pea (Pisum sativum L.) leaf axils contain up to four preformed axillary buds. Decapitating plants above Node 5 promoted the development of preformed buds at all nodes. Buds at each node were removed as soon as they began to grow. These manipulations eventually led to the growth of all the preformed buds and promoted the development of supernumerary buds in the axil of each leaf. Adventitious buds were not observed anywhere on these plants. Examination of leaf axil anatomy and external morphology indicated that new buds were derived from the axillary meristem. The axillary meristem at Node 2 was capable of forming at least six supernumerary buds. ‘Alaska’ plants (Rms-2) exhibited strong apical dominance whereas WL5951 plants (rms-2) contained branches at several nodes. Patterns of bud organogenesis and development were similar in both cultivars.

scholarly journals Efficient Cryopreservation of Populus tremula by In Vitro-Grown Axillary Buds and Genetic Stability of Recovered Plants

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 77
Author(s):  
Elena O. Vidyagina ◽  
Nikolay N. Kharchenko ◽  
Konstantin A. Shestibratov
Keyword(s):  
Survival Rate ◽  
Axillary Buds ◽  
Long Term Storage ◽  
Average Survival ◽  
Transgenic Lines ◽  
Ssr Loci ◽  
One Step ◽  
The One

Axillary buds of in vitro microshoots were successfully frozen at –196 °C by the one-step freezing method using the protective vitrification solution 2 (PVS2). Microshoots were taken from 11 transgenic lines and three wild type lines. Influence of different explant pretreatments were analyzed from the point of their influence towards recovery after cryopreservation. It was found out that the use of axillary buds as explants after removal of the apical one increases recovery on average by 8%. The cultivation on growth medium of higher density insignificantly raises the regenerants survival rate. Pretreatment of the osmotic fluid (OF) shows the greatest influence on the survival rate. It leads to the increase in survival rate by 20%. The cryopreservation technology providing regenerants average survival rate of 83% was developed. It was based on the experimental results obtained with explant pretreatment. Incubation time in liquid nitrogen did not affect the explants survival rate after thawing. After six months cryostorage of samples their genetic variability was analyzed. Six variable simple sequence repeat (SSR) loci were used to analyze genotype variability after the freezing-thawing procedure. The microsatellite analysis showed the genetic status identity of plants after cryopreservation and of the original genotypes. The presence of the recombinant gene in the transgenic lines after cryostorage were confirmed so as the interclonal variation in the growth rate under greenhouse conditions. The developed technique is recommended for long-term storage of various breeding and genetically modified lines of aspen plants, as it provides a high percentage of explants survival with no changes in genotype.

scholarly journals Using Discrete-Point LiDAR to Classify Tree Species in the Riparian Pacific Northwest, USA

2021 ◽  
Vol 13 (14) ◽  
pp. 2647
Author(s):  
Julia Tatum ◽  
David Wallin
Keyword(s):  
Pacific Northwest ◽  
Tree Species ◽  
3D Structure ◽  
Alnus Rubra ◽  
Populus Balsamifera ◽  
Segmentation Method ◽  
Black Cottonwood ◽  
Riparian Landscape ◽  
Individual Trees ◽  
Species Mapping

Practical methods for tree species identification are important for both land management and scientific inquiry. LiDAR has been widely used for species mapping due to its ability to characterize 3D structure, but in structurally complex Pacific Northwest forests, additional research is needed. To address this need and to determine the feasibility of species modeling in such forests, we compared six approaches using five algorithms available in R’s lidR package and Trimble’s eCognition software to determine which approach most consistently identified individual trees across a heterogenous riparian landscape. We then classified segments into Douglas fir (Pseudotsuga menziesii), black cottonwood (Populus balsamifera ssp. trichocarpa), and red alder (Alnus rubra). Classification accuracies based on the best-performing segmentation method were 91%, 92%, and 84%, respectively. To our knowledge, this is the first study to investigate tree species modeling from LiDAR in a natural Pacific Northwest forest, and the first to model Pacific Northwest species at the landscape scale. Our results suggest that LiDAR alone may provide enough information on tree species to be useful to land managers in limited applications, even under structurally challenging conditions. With slight changes to the modeling approach, even higher accuracies may be possible.

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