scholarly journals Corresponding erdosteine changes autophagy genes expression in hippocampus on Rhinitis medicamentosa model

Genetika ◽  
2015 ◽  
Vol 47 (3) ◽  
pp. 1091-1098
Author(s):  
Recep Dokuyucu ◽  
Bulent Gogebakan ◽  
Cengiz Cevik
Keyword(s):  
Control Group ◽  
Gene Expressions ◽  
Autophagy Gene ◽  
Genes Expression ◽  
Left Hippocampus ◽  
Antioxidant Agent ◽  
Group 3 ◽  
Group 2 ◽  
Rhinitis Medicamentosa

In our study, rats were subjected to Oxymetazoline hydrochloride treatment and Rhinitis medicamentosa (RM) was formed and then autophagy gene expression levels were determined after the application of an antioxidant agent erdosteine (ED). The rats were divided into three groups; Group 1 was the control group. Group 2 (RM) and group 3 (RM+ED) rats received two spray puffs of 0.05% oxymetazoline into the nasal cavities three times daily for eight weeks. After determination of RM in the rats, the RM group were killed. The ED+RM group received 10 mg/kg of an ED suspension. At the end of seven days, these rats were also killed. All groups? hippocampus tissues were obtained for the measurement of autophagy gene expressions. In rhinitis medicamentosa group Atg5, Atg7 and Atg10 gene expressions in the left hippocampus were reduced as compared to control group (p=0.01, p>0.05, p=0.01, respectively). Also, erdosteine treatments were restored mRNA expression of autophagy genes. In right hippocampus of rhinitis medicamentosa group, Atg5 and Atg10 gene expressions was found to be down-regulated as compared to control group (p>0.05, p<0.05, respectively). Both BECN1 and ULK genes expression were found to be reduced in left hippocampus of rhinitis medicamentosa group. Erdosteine applications was restored the expression of these genes (p=0.03, p=0.03, respectively). Additionally, in right hippocampus, Erdosteine application was restored the expression of ULK gene (p=0.01). This is the first report that evaluated the expression autophagy genes in RM rat models and the changes observed after erdosteine applications.

scholarly journals Cross Talk Between Down Regulation of Steroidogenic Genes Expression, Oxidative and Apoptotic Biomarkers in Testes Induced by Administration of Tramadol and Boldenone and Their Combination in Male Albino Rats

2021 ◽  
Author(s):  
Gihan F. Asaad ◽  
Noha Mowaad ◽  
Marwa E.A. El-Shamarka ◽  
Sahar Khalil
Keyword(s):  
Control Group ◽  
Albino Rats ◽  
Group 4 ◽  
Vital Functions ◽  
Concurrent Use ◽  
Genes Expression ◽  
Group 3 ◽  
Group 2 ◽  
Oxidative Biomarkers ◽  
Reproductive Gland

Abstract BackgroundThe testis is the male reproductive gland or gonad having two vital functions - to produce both sperm and androgens, primarily testosterone.PurposeThe study aimed to investigate the effect of tramadol and boldenone injected alone or in combinatio for 2 months in rats on testicular function.MethodsGroup 1; normal control, Group 2; tramadol Hcl (TRAM) (20 mg/kg bwt.) (i.p). Group 3; boldenone undecylenate (BOLD) (5 mg/kg bwt) (i.m). Group 4; combination of TRAM (20 mg/kg bwt.) and BOLD (5 mg/kg), respectively for 2 months.ResultsTRAM and BOLD alone and in combination rats showed deteriorated testicular functions, lowered serum steroid levels (FSH, LH and testesterone), elevation in oxidative biomarkers (MDA & NO) and reduction in GSH and SOD, downregulation of StaR and HSD17B3 as well as assessment of testicular histopathological using H&E staining, PAS stain for histochemical assessment of polysaccharides and glycoproteins in the testes and Masson trichrome stain to assess the changes in the collagen fibers.ConclusionThe study illuminated the hazard of administration of these drugs for a long period as well as the prominent deleterious effects reported on concurrent use of both drugs.

scholarly journals Determination of fibrin glue with antibiotics on collagen production in colon anastomosis

2008 ◽  
Vol 65 (9) ◽  
pp. 681-687
Author(s):  
Zoran Stanojkovic ◽  
Goran Stanojevic ◽  
Miroslav Stojanovic ◽  
Dragan Milic ◽  
Sasa Zivic
Keyword(s):  
Fibrin Glue ◽  
Morbidity Rate ◽  
Control Group ◽  
Colon Anastomosis ◽  
Colonic Anastomoses ◽  
Collagen Production ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Background/Aim. Fibrin glue is used as a matrix for local application of antibiotics. The aim of this study was to determine whether application of fibrin glue in combination with antibiotics can strengthen collagen production, prevent dehiscence of colon anastomoses due to infection, and reduce frequency of mortality and morbidity comparing to the control group and the group with fibrin glue application. Methods. The adult male Wistar rats divided into three groups were used in the experiment. The group 1 was the control one (after partial colon resection, colonic anastomoses performed were not treated), while to the group 2 and the group 3 were applied fibrin glue and fibrin glue with antibiotics (clindamycin and ceftriaxon) on the site of anastomoses, respectively. Quality of colonic anastomoses were estimated by means of determination of collagen (L-hydroxyproline) amount in the collon wall with anastomoses and histological analysis of this colon segment using light and electronic microscope on the days 5, 7 and 13 postoperatively. Results. The highest morbidity rate was registered in the group 1 (30%), then in the group 2 (13.3%) and the lowest one in the group 3 (3.33%; p < 0,05 vs group 1). Mortality rate was significantly higher in the group 1 than in the group 3 (20% and 0%, respectively; p < 0,05). In the postoperative course, the highest concentrations of collagen in the colon wall on the site of anastomoses, which was confirmed by both light and electronic microscopy, were found in the group 3. Conclusion. The application of fibrin glue with antibiotics on colon anastomoses reduces the number of dehiscence, provides good mechanical protection and shorten the time of anastomoses healing.

scholarly journals OXIDATIVE STATUS OF BLOOD PLASMA IN REFRACTORY PREGNANCY LOSS

2017 ◽  
Vol 4 (4) ◽  
pp. 214-219
Author(s):  
Marya Sh. Pokaleneva ◽  
A. M Nesterova ◽  
E. A Sosnova ◽  
S. B Bolevich ◽  
E. V Proskurnina
Keyword(s):  
Blood Plasma ◽  
Antioxidant Capacity ◽  
Pregnant Women ◽  
Tryptophan Fluorescence ◽  
Normal Pregnancy ◽  
Oxidative Modification ◽  
Control Group ◽  
Group 3 ◽  
Group 2

The habitual miscarriage of the pregnancy is one of the most urgent problems in obstetrics and gynecology. There was performed a study of the antioxidant activity of blood plasma by kinetic chemiluminescence, the determination of tryptophan fluorescence was used to evaluate the oxidative modification of albumin. The study included 28 pregnant women with the threat of abortion (TA), 19 of them had a diagnosis of habitual miscarriage, and 9 women - an undeveloped pregnancy. The control group 1 included 61 women with normal pregnancy, the control group 2 - 22 non-pregnant women, control group 3 - 5 women after spontaneous miscarriage (up to 22 weeks).The results showed spontaneous abortion to be followed by the decline in the antioxidant capacity of the plasma, which indicates to the development of oxidative stress; on the contrary, in women with an undeveloped pregnancy, the antioxidant capacity was higher. In the determination of the proportion of oxidized albumin in all patients, but nonpregnant women, there were noted negative values indicating to the presence of specific antibodies or, possibly, cytokines possessing tryptophan fluorescence.

Active head movements facilitate compensation for effects of prism displacement on dynamic gait12

2006 ◽  
Vol 16 (1-2) ◽  
pp. 29-33
Author(s):  
Kim R. Gottshall ◽  
Michael E. Hoffer ◽  
Helen S. Cohen ◽  
Robert J. Moore
Keyword(s):  
Head Movement ◽  
Sensory Modality ◽  
Normal Subjects ◽  
The Body ◽  
Head Movements ◽  
Head Motion ◽  
Control Group ◽  
Entire Body ◽  
Group 3 ◽  
Group 2

Study design: Four groups, between-subjects study. Objectives: To investigate the effects of exercise on adaptation of normal subjects who had been artificially spatially disoriented. Background: Many patients referred for rehabilitation experience sensory changes, due to age or disease processes, and these changes affect motor skill. The best way to train patients to adapt to these changes and to improve their sensorimotor skills is unclear. Using normal subjects, we tested the hypothesis that active, planned head movement is needed to adapt to modified visual input. Methods and measures: Eighty male and female subjects who had normal balance on computerized dynamic posturography (CDP) and the dynamic gait index (DGI), were randomly assigned to four groups. All groups donned diagonally shift lenses and were again assessed with CDP and DGI. The four groups were then treated for 20 min. Group 1 (control group) viewed a video, Group 2 performed exercise that involved translating the entire body through space, but without separate, volitional head movement, Group 3 performed exercises which all incorporated volitional, planned head rotations, and Group 4 performed exercises that involved translating the body (as in Group 2) and incorporated volitional, planned head motion (as in Group 3). All subjects were post-tested with CDP and DGI, lenses were removed, and subjects were retested again with CDP and DGI. Results: The groups did not differ significantly on CDP scores but Groups 3 and 4 had significantly better DGI scores than Groups 1 and 2. Conclusions: Active head movement that is specifically planned as part of the exercise is more effective than passive attention or head movements that are not consciously planned, for adapting to sensorimotor change when it incorporates active use of the changed sensory modality, in this case head motion.

scholarly journals Preemptive analgesic effect of lidocaine in a chronic neuropathic pain model

2009 ◽  
Vol 67 (4) ◽  
pp. 1088-1092 ◽  
Author(s):  
Leonardo M. Batista ◽  
Igor M. Batista ◽  
João P. Almeida ◽  
Carlos H. Carvalho ◽  
Samuel B. de Castro-Costa ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Control Group ◽  
Thermal Stimulus ◽  
Lidocaine Group ◽  
Local Infiltration ◽  
Neuropathic Pain Model ◽  
Group 3 ◽  
Group 2 ◽  
The Right

Preemptive analgesia inhibits the progression of pain caused by surgical lesions. To analyze the effect of lidocaine on postoperative pain relief, we performed compression of the right sciatic nerve in Wistar rats and observed the differences on behavior between the group that received lidocaine and the group that was not treated with the local anesthetics pre-operatively. Group 1 was not operated (control); group 2 underwent the sciatic nerve ligature without lidocaine; group 3, underwent surgery with previous local infiltration of lidocaine. Group 2 showed significantly longer scratching times with a peak on day 14 post-operative (p=0.0005) and reduction in the latency to both noxious (p=0.003) and non-noxious (p=0.004) thermal stimulus. Group 3 presented significantly shorter scratching times (p=0.004) and longer latency times when compared to Group 2. Preemptive use of lidocaine 2% can potentially reduce the postoperative neuropathic pain associated with sciatic nerve compression.

Postoperative Morphine Use and Hyperalgesia Are Reduced by Preoperative but Not Intraoperative Epidural Analgesia

2003 ◽  
Vol 98 (6) ◽  
pp. 1449-1460 ◽  
Author(s):  
Joel Katz ◽  
Lorenzo Cohen ◽  
Roger Schmid ◽  
Vincent W.S. Chan ◽  
Adarose Wowk
Keyword(s):  
Morphine Consumption ◽  
Gynecologic Surgery ◽  
Control Group ◽  
Epidural Administration ◽  
Blinded Manner ◽  
Group 3 ◽  
Group 2 ◽  
Double Blinded ◽  
Postoperative Morphine ◽  
Group 1

Background The aim of this study was to evaluate the postoperative morphine-sparing effects and reduction in pain and secondary mechanical hyperalgesia after preincisional or postincisional epidural administration of a local anesthetic and an opioid compared with a sham epidural control. Methods Patients undergoing major gynecologic surgery by laparotomy were randomly assigned to three groups and studied in a double-blinded manner. Group 1 received epidural lidocaine and fentanyl before incision and epidural saline 40 min after incision. Group 2 received epidural saline before incision and epidural lidocaine and fentanyl 40 min after incision. Group 3 received a sham epidural control (with saline injected into a catheter taped to the back) before and 40 min after incision. All patients underwent surgery with general anesthesia. Results One hundred forty-one patients completed the study (group 1, n = 45; group 2, n = 49; group 3, n = 47). Cumulative patient-controlled analgesia morphine consumption at 48 h was significantly lower (P = 0.04) in group 1 (89.8 +/- 43.3 mg) than group 3 (112.5 +/- 71.5 mg) but not group 2 (95.4 +/- 60.2 mg), although the hourly rate of morphine consumption between 24 and 48 h after surgery was significantly lower (P &lt; 0.0009) in group 1 (1.25 +/- 0.02 mg/h) than group 2 (1.41 +/- 0.02 mg/h). Twenty-four hours after surgery, the visual analog scale pain score on movement was significantly less intense (P = 0.005) in group 1 (4.9 +/- 2.2 cm) than group 3 (6.0 +/- 2.6 cm) but not group 2 (5.3 +/- 2.5 cm), and the von Frey pain threshold near the wound was significantly higher (P = 0.03) in group 1 (6.4 +/- 0.6 log mg) than in group 3 (6.1 +/- 0.8 log mg) but not group 2 (6.2 +/- 0.7 log mg). Conclusions Preincisional administration of epidural lidocaine and fentanyl was associated with a significantly lower rate of morphine use, lower cumulative morphine consumption, and reduced hyperalgesia compared with a sham epidural condition. These results highlight the importance of including a standard treatment control group to avoid the problems of interpretation that arise when two-group studies of preemptive analgesia (preincisional vs. postsurgery) fail to find the anticipated effects.

scholarly journals Antioxidant Effect of MnTE-2-PyP on Lung in Asthma Mice Model

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Lyudmil Terziev ◽  
Violeta Dancheva ◽  
Veneta Shopova ◽  
Galya Stavreva
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Control Group ◽  
Mice Model ◽  
Antioxidant Defence ◽  
Group 4 ◽  
Antioxidant Defence System ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Aim. To investigate the effects of MnTE-2-PyP on some markers of antioxidant defence system in asthma mice model.Material and Methods. The animals were divided into four groups: group 1, controls; group 2, injected with ovalbumin, group 3, treated with MnTE-2-PyP, and group 4, treated with ovalbumin and MnTE-2-PyP. The activities of superoxide dismutase, catalase, glutathione peroxidase and nonprotein sulfhydryl groups content (NPSH) were determined in lung homogenate.Results. The activities of superoxide dismutase and catalase in group 2 decreased significantly as compared to control group. The decrease of the same enzymes in group 4 was lower and significant as compared to group 2. Changes in the glutathione peroxidase activity showed a similar dynamics. The NPSH groups content decreased in group 2. In group 4 this decrease was relatively lower as compared to group 2.Conclusions. The application of MnTE-2-PyP mitigated the effects of oxidative stress in asthma mice model.

scholarly journals Effectiveness of medical treatment in overcoming the ureteral double-J stent related symptoms

2012 ◽  
Vol 6 (6) ◽  
pp. 234 ◽  
Author(s):  
Ugur Kuyumcuoglu ◽  
Bilal Eryildirim ◽  
Murat Tuncer ◽  
Gokhan Faydaci ◽  
Fatih Tarhan ◽  
...  
Keyword(s):  
The Body ◽  
Control Group ◽  
Group 4 ◽  
Double J Stent ◽  
Urinary Tract Symptoms ◽  
Stone Treatment ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

Background: We investigated whether the frequency of lower urinary tract symptoms (LUTS) increased in patients in whom double-J stents were applied. We also evaluated several medical therapy protocols to treat symptoms related with ureteral stents.Materials and Methods: A total of 108 patients, in whom unilateral double-j stent was applied during ureteral stone treatment, were included. Before the double-J stent was applied, all patients completed storage components of the “International Prostate Symptom Score” (IPSSs), quality of life components of the IPSS (IPSS-QOL) and “Overactive Bladder Questionnaire” (OABq) forms and scores were calculated. After the procedure, cases were randomized into 5 groups, an antiinflammatory was given to Group 1, spasmolytic to Group 2, anticholinergic to Group 3 and α-blocker to Group 4. No additional drug was given to Group 5 as this control group. During the fourth week of the procedure, IPSSs, IPSS-QOL and OABq forms were again completed and scores were compared with the previous ones.Results: When all the cases were evaluated, the IPSSs, IPSS-QOLand OABq scores of patients in whom the double-J stent was applied were statistically significantly higher the procedure. Compared to the control group, the cases where the double-J stent was applied showed a higher IPSSs, IPSS-QOL and OABq scores and none of the medical therapies could prevent this increase.Interpretation: The frequency of LUTS increased in cases where the ureteral stent was applied and discomfort continued as long as the stent stayed in the body.

scholarly journals Hibiscus sabdariffa Aqueous Leaf Extract Reverses Hematological Alterations in Phenylhydrazine Anemic Wistar Rats

2020 ◽  
pp. 30-38
Author(s):  
E. B. Umoren ◽  
T. A. Kolawole ◽  
I. Wopara ◽  
O. G. Adebayo ◽  
B. Ben-Azu ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Leaf Extract ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Hibiscus Sabdariffa ◽  
Aqueous Leaf Extract ◽  
Group 3 ◽  
Group 2 ◽  
Hb Concentration

Background: The extract of Hibiscus sabdariffa commonly known as Sobo is widely consumed for its nutritional and health benefits. This study investigated the effect of aqueous leaf extract of  H. sabdariffa on anemic condition caused by phenylhydrazine in rats. Materials and Methods: Thirty (30) rats used for this study were divided into three groups of 10 rats each. Group 1 received distilled water and served as control. Group 2 received phenylhydrazine (40 mg/kg, i.p.). Group 3 was treated with phenylhydrazine (40 mg/kg, i.p.) 30 minutes prior to administration of (200 mg/kg, p.o.) aqueous extract of H. sabdariffa (Sobo) once daily for 14 days. At the end, 2 ml blood samples were collected through cardiac puncture into clean sample bottles containing ethylenediamine tetra acetic acid (EDTA) for hematological analyses. Results: Sobo significantly increased (P<0.05) hemoglobin (Hb) concentration and packed cell volume (PCV) in the phenylhdrazine-treated rats. Also, Sobo significantly (P<0.05) increased total white blood cell (TWBC) in phenylhydrazine administered rats. However, the extract did not produce any significant effect on mean corpuscular hemoglobin concentration (MCHC) relative to control and anemic groups. Conclusion: The findings from this study revealed that the aqueous extract of H. sabdariffa demonstrates anti-anemic effect in rats treated with phenylhydrazine, suggesting its ethno-pharmacological beneficial effect in anemic conditions.

scholarly journals Pengaruh Monoklonal Antibodi Bovine Zona Pelusida 3 (bZP3) terhadap Diameter dan Atresia Folikel Ovarium Mencit (Mus musculus)

2018 ◽  
Vol 24 (1) ◽  
pp. 37
Author(s):  
Annisa Trissatharra ◽  
Sri Ratna Dwiningsih ◽  
Ratna Sofaria Munir
Keyword(s):  
Mus Musculus ◽  
Ovarian Follicles ◽  
Observation Time ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Objectives: To identify the effect of monoclonal antibody bZP3 at ovarian follicles that undergo atresia and diameter of various ovarian follicles.Materials and Methods: This is a true experimental research with post only control group design. Samples were 36 female mices (Mus musculus) which is divided into 6 groups, there are 3 control groups (group 1, 2, and 3) injected by Phospatase Buffer Saline (PBS) 50µl and 3 treatment groups (group 4, 5, and 6) injected by Mab bZP3 50µl. Group 1 and 4 terminated at 5th day, group 2 and 5 terminated at 10th day, and group 3 and 6 terminated at 20th day. Evaluation of atretic ovarian follicles and diameter of ovarian follicles performed by hematoxylin eosin (HE) and the data processed by parametric statistic.Results: There are no significant in different among groups in the aspect of atretic follicles and diameter of folicles (p>0.05), but descriptively, number of follicles undergo atresia of the follicle primary, secondary, and tertiary treatment group was higher than the control group, except on the 20th day of observation time.Conclusion: administration of Mab bZP3 had no effect to amount of atretic follicles and diameter of folicles during observation time.

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