The effects of casing soil treatment with bacillus subtilis Ch-13 biofungicide on green mould control and mushroom yield

The impact of a biofungicide based on Bacillus subtilis Ch-13 on mushroom yield and efficacy in suppression of Trichoderma aggressivum f. europaeum T77 from Serbia was estimated in comparision with a similar microbial fungicide, Bacillus velezensis QST713, and the chemical fungicide prochloraz manganese. The biofungicide B. velezensis QST713 is registered for treatments of mushrooms and other crops in many countries but it is not currently available on the Serbian market. The tested B. subtilis Ch-13 fungicide enhanced mushroom yield 12%, compared with an uninoculated control, and notably more than B. velezensis QST713 applied at its higher test concentrations. Regarding the efficacy of the biofungicides in control of the compost pathogen T. aggressivum f. europaeum, B. subtilis Ch-13 applied in concentration of 3 ? 108 CFU per m2 showed higher efficacy than the higher concentrations (5 ? 109 and 1 ? 1010 CFU per m2) of B. velezensis QST713. The biofungicide based on B. subtilis Ch-13 should be further investigated regarding its different modes of application to ensure better efficacy in disease control as it showed beneficial features in both promoting A. bisporus production and suppressing the growth of the aggressive compost pathogen T. aggressivum, the causal agent of devastating green mould disease.

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A large-scale study on the effectiveness of a Bacillus subtilis Ch-13-based biofungicide against green mould disease and mushroom yield improvement

Pesticidi i fitomedicina ◽

10.2298/pif2102083p ◽

2021 ◽

Vol 36 (2) ◽

pp. 83-90

Author(s):

Ivana Potocnik ◽

Biljana Todorovic ◽

Svetlana Milijasevic-Marcic ◽

Jelena Lukovic ◽

Gabriella Kanizai-Saric ◽

...

Keyword(s):

Bacillus Subtilis ◽

Untreated Control ◽

Agaricus Bisporus ◽

Large Scale ◽

Natural Infection ◽

Subtilis Strain ◽

Cultivated Mushroom ◽

Green Mould ◽

Mushroom Yield ◽

Final Amount

The aim of this study was to test a biofungicide based on Bacillus subtilis Ch-13 and its effectiveness in the control of green mould disease of cultivated mushroom in comparison with the fungicide prochloraz. Biofungicide effectiveness in disease control and impact on yield were evaluated on Agaricus bisporus after its natural infection with Trichoderma aggressivum in a commercial mushroom growing facility. An assay for testing the microbial efficacy of the biofungicide was conducted in two different procedures involving either three or two split doses. The highest statistically significant effectiveness in green mould control was shown by the fungicide prochloraz (71.43%), followed by the biofungicide applied in tree split doses (53.57%), and finally its two doses (45.46%). The biofungicide significantly improved yield in comparison with an untreated control and the fungicide prochloraz. Three split applications of B. subtilis strain Ch-13 enhanced mushroom yield to a larger extent than its two split doses, although the same final amount was used in both procedures. Biofungicide application in three split doses increased the total mass of harvested mushrooms 8.41% compared to the untreated control, and 10.53% compared to the fungicide prochloraz. These results implied that the biofungicide should be applied in three split applications: 30 ml (second day after casing) + 15 ml (two weeks after casing) + 15 ml (after first flush, 20-25 days after casing). The biofungicide B. subtilis Ch-13 should be further investigated regarding its joint usage with chemical fungicides in different application procedures, as it showed remarkable characteristics both in terms of promoting mushroom yield and inhibiting the spread of mycopathogenic T. aggressivum.

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Adaptive Reactions of Winter Wheat (Triticum aestivum L.) Affected by Eyespot Causal Agent under the Action of Bacillus subtilis Bacterial Isolates

Mikrobiolohichnyi Zhurnal ◽

10.15407/microbiolj81.01.049 ◽

2019 ◽

Vol 81 (1) ◽

pp. 49-60

Author(s):

L.M. Batsmanova ◽

◽

Y.M. Pysmenna ◽

T.O. Kondratiuk ◽

N.Y. Taran ◽

...

Keyword(s):

Triticum Aestivum ◽

Bacillus Subtilis ◽

Winter Wheat ◽

Triticum Aestivum L ◽

Causal Agent ◽

Bacterial Isolates ◽

Adaptive Reactions

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Effects of Bacillus velezensis on Fusarium avenaceum, a causal agent of post-harvest apple fruit rot

10.26226/morressier.5f97e7baaee6041e8a666207 ◽

2020 ◽

Author(s):

Mladen Petres ◽

Marta Loc ◽

Mila Grahovac ◽

Vera Stojsin ◽

Dragana Budakov ◽

...

Keyword(s):

Apple Fruit ◽

Causal Agent ◽

Fruit Rot ◽

Fusarium Avenaceum ◽

Bacillus Velezensis ◽

Post Harvest

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Lectin activity of winter wheat seedlings affected by eyespot causal agent under the action of Bacillus subtilis 537/Б1 bacterial isolates

Visnyk agrarnoi nauky ◽

10.31073/agrovisnyk201810-04 ◽

2018 ◽

Vol 96 (10) ◽

pp. 27-34

Author(s):

M. Musiienko ◽

L. Batsmanova ◽

Ju. Pys'menna ◽

T. Kondratiuk ◽

N. Taran ◽

...

Keyword(s):

Bacillus Subtilis ◽

Winter Wheat ◽

Causal Agent ◽

Bacterial Isolates ◽

Lectin Activity ◽

Wheat Seedlings

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Confronting Two Crises: The COVID-19 Pandemic, the Opioid Epidemic, and the Industrial Hygienist

NEW SOLUTIONS A Journal of Environmental and Occupational Health Policy ◽

10.1177/10482911211029580 ◽

2021 ◽

pp. 104829112110295

Author(s):

Jonathan Rosen ◽

Peter Harnett

Keyword(s):

Disease Control ◽

Drug Overdose ◽

Industrial Hygiene ◽

Prevention Programs ◽

Opioid Epidemic ◽

Opioid Misuse ◽

Opioid Crisis ◽

The Impact ◽

Overdose Deaths ◽

Industrial Hygienist

This article was originally written for and published in the January 2021 issue of The Synergist, a monthly publication of the American Industrial Hygiene Association. The article addresses the convergence of the COVID-19 and opioid crises, the impact of the opioid crisis on the workplace and workers, and the role that industrial hygienists can play in developing workplace programs to prevent and respond to opioid misuse. While the article is specifically written for industrial hygienists, the review and recommendations will be useful to others who are developing workplace opioid prevention programs. Note that the data presented in this article were current as of January 2021. Centers for Disease Control and Prevention’s latest available data are for the twelve-month period ending October 2020 and include 88,990 total overdose deaths and 91,862 predicted, when reporting is completed. Source: https://www.cdc.gov/nchs/nvss/vsrr/drug-overdose-data.htm (accessed on 15 June 2021).

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129 Effects of a multi-strain Bacillus subtilis-based direct-fed microbial on growth performance, nutrient digestibility, and colonic pH in diets fed to weanling pigs

Journal of Animal Science ◽

10.1093/jas/skaa278.185 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 101-102

Author(s):

Jaron R Lewton ◽

Adrienne Woodward ◽

Ronny Moser ◽

Kyan M Thelen ◽

Adam J Moeser ◽

...

Keyword(s):

Amino Acids ◽

Bacillus Subtilis ◽

Growth Performance ◽

Distal Colon ◽

Nutrient Digestibility ◽

Nursery Pigs ◽

Fiber Digestion ◽

Males And Females ◽

Dietary Treatments ◽

The Impact

Abstract A study was conducted to evaluate the effects of a multi-strain Bacillus subtilis-based direct-fed microbial (DFM) on apparent digestibility and colonic pH of nursery pigs. Eighty pigs, of equal number of barrows and gilts (initial BW: 6.99 ± 1.67 kg), were weaned at 21 ± 1 d and randomly allotted to sixteen pens, with five pigs per pen. Two dietary treatments were implemented, a basal control (CON) and a control plus DFM (DFM). Both diets were corn, soybean meal, and distillers dried grains based, formulated to meet all or exceed all nutritional requirements, and manufactured on site. Diets were fed for 42 days. Performance measures were recorded weekly. On d 21 and 42 of the experiment, one pig per pen was randomly selected and euthanized, with equal number of males and females represented. Digestibility of specific nutrients was evaluated within the duodenum, jejunum, ileum, ascending and distal colon. There were no overall differences in growth performance. Overall means ± SD were 0.51 ± 0.05 kg/d, 0.79 ± 0.05 kg/d and 0.66 ± 0.05 for ADG, ADFI, and G:F, respectively. Digestibility of tryptophan within the jejunum tended (P = 0.06) to increase with addition of DFM, as did cysteine (P = 0.12) and methionine (P = 0.10). The analysis also suggested that the impact of the DFM on the digestibility of amino acids may be early in the nursery phase. The pH of contents in ascending colon, a possible indicator of varied fiber digestion, did not differ. Likewise, no differences were observed between treatment in apparent total tract nitrogen and energy digestibility (analysis of distal colon contents). The addition of a multi-strain Bacillus subtilis-based DFM appears to impact digestibility of select amino acids depending upon location in the gastrointestinal tract.

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Combined Application of Rhizosphere Bacteria with Endophytic Bacteria Suppresses Root Diseases and Increases Productivity of Black Pepper (Piper nigrum L.)

Agriculture ◽

10.3390/agriculture11010015 ◽

2020 ◽

Vol 11 (1) ◽

pp. 15

Author(s):

Sy Dinh Nguyen ◽

Thi Huyen Trang Trinh ◽

Trung Dzung Tran ◽

Tinh Van Nguyen ◽

Hoang Van Chuyen ◽

...

Keyword(s):

Bacillus Subtilis ◽

Endophytic Bacteria ◽

Growth Promotion ◽

Black Pepper ◽

Rhizosphere Bacteria ◽

Growth And Yield ◽

Piper Nigrum ◽

Bacillus Velezensis ◽

Root Knot Nematode ◽

Central Highland

Black pepper (Piper nigrum L.) is one of the most important crops and global demand continues to increase, giving it a high export value. However, black pepper cultivation has been seriously affected by a number of pathogenic diseases. Among them, “quick wilt” caused by Phytophthora sp., “slow decline” caused by Fusarium sp., and root-knot nematode Meloidogyne sp. have a serious negative effect on black pepper growth and productivity. There have been different chemical and biological methods applied to control these diseases, but their effectiveness has been limited. The aim of this research was to evaluate different combinations of rhizosphere bacteria and endophytic bacteria isolated from black pepper farms in the Central Highland of Vietnam for their ability to suppress pathogens and promote black pepper growth and yield. Formula 6, containing the strains Bacillus velezensis KN12, Bacillus amyloliquefaciens DL1, Bacillus velezensis DS29, Bacillus subtilis BH15, Bacillus subtilis V1.21 and Bacillus cereus CS30 exhibited the largest effect against Phytophthora and Fusarium in the soil and in the roots of black pepper. These bio-products also increased chlorophyll a and b contents, which led to a 1.5-fold increase of the photosynthetic intensity than the control formula and a 4.5% increase in the peppercorn yield (3.45 vs. 3.30 tons per hectare for the control). Our results suggest that the application of rhizosphere and endophytic bacteria is a promising method for disease control and growth-promotion of black pepper.

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Radiosurgery and stereotactic radiotherapy with cyberknife system for meningioma treatment

The Neuroradiology Journal ◽

10.1177/1971400917744885 ◽

2017 ◽

Vol 31 (1) ◽

pp. 18-26 ◽

Cited By ~ 10

Author(s):

Rossella Di Franco ◽

Valentina Borzillo ◽

Vincenzo Ravo ◽

Sara Falivene ◽

Francesco Jacopo Romano ◽

...

Keyword(s):

Disease Control ◽

Stereotactic Radiosurgery ◽

Local Control ◽

Stereotactic Radiotherapy ◽

Histological Grade ◽

Fractionated Stereotactic Radiotherapy ◽

Subtotal Resection ◽

Local Disease ◽

Local Disease Control ◽

The Impact

Objective The aim of this work was to evaluate the impact of stereotactic radiosurgery/fractionated stereotactic radiotherapy with the Cyberknife system on local disease control, clinical outcome and toxicity in patients with meningioma, according to the site and histological grade of lesion. From January 2013 to April 2017, 52 patients with intracranial meningiomas were treated with the Cyberknife system. Twenty-four patients had undergone previous surgery: 38% gross total resection, 10% subtotal resection; 27 patients underwent no surgery; 22 patients had a recurrence of meningioma. Methods Radiosurgery was used for lesions smaller than 2 cm, stereotactic radiotherapy for lesions larger than 2 cm, or smaller but close to a critical site such as the optical chiasm, optic pathway or brainstem. Results Local control and clinical outcomes were analysed. Median follow-up was 20 months: six patients died, one after re-surgery died from post-surgical sepsis, three from heart disease. Progression-free survival had a mean value of 38.3 months and overall survival of 41.6 months. We evaluated at 12 months 28 patients (100% local control); at 24 months 19 patients (89% local control); at 36 months nine patients (89% local control). At baseline, 44/52 patients (85%) were symptomatic: 19 visual disorders, 17 motor disorders, six hearing disorders, 10 headache and six epilepsy. Visual symptoms remained unchanged in 52%, improved in 32%, resolved in 16%. Headache was improved in 40%, resolved in 10%, unchanged in 50%. Epilepsy was resolved in 17%, unchanged in 33%, worsened in 33%. Conclusions Stereotactic radiosurgery/fractionated stereotactic radiotherapy with Cyberknife provides a good local disease control, improving visual, hearing and motor symptoms.

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