scholarly journals Active constituents of Litsea cubeba

2020 ◽  
Vol 24 (3 Part A) ◽  
pp. 1745-1752
Author(s):  
Quan Zhou ◽  
Dongfang Zheng ◽  
Yong Zhao ◽  
Ting Wang ◽  
Yafeng Yang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Large Scale ◽  
Gas Chromatography Mass Spectrometry ◽  
Gravimetric Analysis ◽  
Litsea Cubeba ◽  
Chromatography Mass Spectrometry ◽  
Wide Range ◽  
Large Scale Cultivation ◽  
Active Substances

Litsea cubeba is a plant of Lauraceae and Litsea. It is a valuable plant and has a wide range of uses, including in traditional Chinese medicine. Herein, Litsea cubeba wood was harvested from Henan Province, The active ingredients were extracted from Litsea cubeba wood by modern techniques such as gas chromatography-mass spectrometry (GC-MS), thermal gravimetric analysis, and thermal desorption gas chromatography-mass spectrometry. The analysis results show that the wood of Litsea cubeba contains a large amount of valuable active substances that can be utilized in medicine, bio-energy, and spices and flavorings, and large-scale cultivation of this plant could be beneficial.

scholarly journals EVALUATION OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND CYTOTOXICITY OF ETHANOLIC LEAF EXTRACT OF ACACIA CAESIA (L.) WILLD. AGAINST HELA CELL LINE

2019 ◽  
Vol 12 (1) ◽  
pp. 520
Author(s):  
Sharmila S ◽  
Ramya E K
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Leaf Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Wide Range ◽  
Ethanolic Leaf Extract

Objective: The objective of this study is to analyze the bioactive compounds of the ethanolic leaf extract of Acacia caesia using gas chromatography-mass spectrometry (GC-MS) method and also screen the in vitro cytotoxic activity against HeLa-E 72 cancer cell line.Methods: The present research was carried out using GC-MS analysis, while mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology and Wiley library. Cytotoxicity was assessed with 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide assay, and cellular morphological alterations were studied using phase contrast inverted light microscope of 400×. The ethanol extract of A. caesia was screened for their cytotoxicity at different concentrations (12.5–200 μg/ml), to determine the mean percentage (%) cell viability.Results: The results of GC/MS analysis showed the presence of 41 major compounds. In terms of percentage amounts, 1,8-diphenyl-3,4,10,11- tetrahydro[1,4]dioxino[2,3-g:5,6-g’]diisoquinoline, 6-(chloromethyl)-4-(3,4-dimethoxy-2-(phenylmethoxy)-phenyl)-3-methyl-2-yridinecarboxylate, and 2’,4’,6’-Trinitro-5’-phenyl-1,1’:3’,1”-terphenyl were predominant in the extract and have the property of antioxidant, antidepressant potential, antibacterial activity, cytotoxic, diabetic, and induced brain activity. The results of cytotoxicity at highest concentration (200 μg/ml) of the cells became rounder, shrunken and showed signs of detachment from the surface of the wells denoting cell death.Conclusions: From this study, it is obvious that A. caesia leaf extracts contain various bioactive constituents with a wide range of medicinal properties which is used to treat multiple disorders and it also gives a detailed insight about the phytochemical profile which could be exploited for the development of plant-based drugs. Further, the ethanolic extract of A. caesia exhibits potent cytotoxic activity against HeLa-E 72 cell line.

scholarly journals Lowering cutoffs for initial and confirmation testing for cocaine and marijuana: large-scale study of effects on the rates of drug-positive results

1997 ◽  
Vol 43 (1) ◽  
pp. 100-103 ◽  
Author(s):  
William E Wingert
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Large Scale ◽  
Gas Chromatography Mass Spectrometry ◽  
Initial Testing ◽  
Chromatography Mass Spectrometry ◽  
Large Scale Study ◽  
Positive Rate ◽  
Positive Results

Abstract A large-scale study was conducted to determine whether lowering the initial testing and confirmation testing cutoffs in urine would significantly affect the positive rates for cocaine (COC) and marijuana (THC). Customary cutoffs for COC are 300 μg/L and 150 μg/L for initial testing (screening) and gas chromatography–mass spectrometry (GC-MS; confirmation), respectively; for THC, the usual respective cutoffs are 50 μg/L and 10 μg/L. By applying a screening cutoff of 100 μg/L for COC and lowering the GC-MS cutoff to 50 μg/L, the COC-positive rate increased from 1.2% to 2.1%. For THC, lowering the screening cutoff to 20 μg/L while leaving the GC-MS cutoff at 10 μg/L increased the THC-positive rate from 2.8% to 4.1%. These increases appear noteworthy.

Determining wildland fire markers in residential structures using thermal desorption gas chromatography mass spectrometry

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
M. Katie Martin ◽  
Dale J. LeCaptain ◽  
Alice Delia
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Thermal Desorption ◽  
Wildland Fire ◽  
The United States ◽  
Gas Chromatography Mass Spectrometry ◽  
Organic Chemical ◽  
Wildland Fires ◽  
Chromatography Mass Spectrometry ◽  
Wide Range

Abstract Each year thousands of wildland fires blaze across the United States causing secondary (“smoke”) damage to numerous businesses and personal property. Currently there are no specific industry standards or guidelines for determining wildfire combustion residues. Remediation decisions often rely on anecdotal evidence from occupants. A variety of particulate methods are used to assess surface contamination but there are few methods for evaluating organic chemical residues that encompass the wide range of chemical classes produced during wildland fires. A new method was developed employing a thermal desorption gas chromatography mass spectrometry (TD-GCMS). TD-GCMS using novel sorbent beds decreases the sample preparation substantially and enables sampling of bulk materials by off-gassing. Furthermore, the method developed is specific to wildland fire events. Results from a simulated wildland fire event are also provided.

scholarly journals EVALUATION OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND CYTOTOXICITY OF ETHANOLIC LEAF EXTRACT OF ACACIA CAESIA (L.) WILLD. AGAINST HELA CELL LINE

2019 ◽  
Vol 12 (1) ◽  
pp. 520
Author(s):  
Sharmila S ◽  
Ramya E K
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Leaf Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Wide Range ◽  
Ethanolic Leaf Extract

Objective: The objective of this study is to analyze the bioactive compounds of the ethanolic leaf extract of Acacia caesia using gas chromatography-mass spectrometry (GC-MS) method and also screen the in vitro cytotoxic activity against HeLa-E 72 cancer cell line.Methods: The present research was carried out using GC-MS analysis, while mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology and Wiley library. Cytotoxicity was assessed with 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide assay, and cellular morphological alterations were studied using phase contrast inverted light microscope of 400×. The ethanol extract of A. caesia was screened for their cytotoxicity at different concentrations (12.5–200 μg/ml), to determine the mean percentage (%) cell viability.Results: The results of GC/MS analysis showed the presence of 41 major compounds. In terms of percentage amounts, 1,8-diphenyl-3,4,10,11- tetrahydro[1,4]dioxino[2,3-g:5,6-g’]diisoquinoline, 6-(chloromethyl)-4-(3,4-dimethoxy-2-(phenylmethoxy)-phenyl)-3-methyl-2-yridinecarboxylate, and 2’,4’,6’-Trinitro-5’-phenyl-1,1’:3’,1”-terphenyl were predominant in the extract and have the property of antioxidant, antidepressant potential, antibacterial activity, cytotoxic, diabetic, and induced brain activity. The results of cytotoxicity at highest concentration (200 μg/ml) of the cells became rounder, shrunken and showed signs of detachment from the surface of the wells denoting cell death.Conclusions: From this study, it is obvious that A. caesia leaf extracts contain various bioactive constituents with a wide range of medicinal properties which is used to treat multiple disorders and it also gives a detailed insight about the phytochemical profile which could be exploited for the development of plant-based drugs. Further, the ethanolic extract of A. caesia exhibits potent cytotoxic activity against HeLa-E 72 cell line.

scholarly journals WiPP: Workflow for Improved Peak Picking for Gas Chromatography-Mass Spectrometry (GC-MS) Data

Metabolites ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 171 ◽  
Author(s):  
Borgsmüller ◽  
Gloaguen ◽  
Opialla ◽  
Blanc ◽  
Sicard ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Large Scale ◽  
Automated Analysis ◽  
Peak Detection ◽  
Performance Comparison ◽  
Gas Chromatography Mass Spectrometry ◽  
Peak Picking ◽  
Chromatography Mass Spectrometry ◽  
Biological Dataset

Lack of reliable peak detection impedes automated analysis of large-scale gas chromatography-mass spectrometry (GC-MS) metabolomics datasets. Performance and outcome of individual peak-picking algorithms can differ widely depending on both algorithmic approach and parameters, as well as data acquisition method. Therefore, comparing and contrasting between algorithms is difficult. Here we present a workflow for improved peak picking (WiPP), a parameter optimising, multi-algorithm peak detection for GC-MS metabolomics. WiPP evaluates the quality of detected peaks using a machine learning-based classification scheme based on seven peak classes. The quality information returned by the classifier for each individual peak is merged with results from different peak detection algorithms to create one final high-quality peak set for immediate down-stream analysis. Medium- and low-quality peaks are kept for further inspection. By applying WiPP to standard compound mixes and a complex biological dataset, we demonstrate that peak detection is improved through the novel way to assign peak quality, an automated parameter optimisation, and results in integration across different embedded peak picking algorithms. Furthermore, our approach can provide an impartial performance comparison of different peak picking algorithms. WiPP is freely available on GitHub (https://github.com/bihealth/WiPP) under MIT licence.

scholarly journals An Effective and Efficient Sample Preparation Method for 2-Methyl-Isoborneol and Geosmin in Fish and Their Analysis by Gas Chromatography-Mass Spectrometry

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Liang-liang Tian ◽  
Feng Han ◽  
Essy Kouadio Fodjo ◽  
Wenlei Zhai ◽  
Xuan-Yun Huang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Sample Preparation ◽  
Large Scale ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Fish Samples

The intensive aquaculture strategy and recirculating aquaculture system often lead to the production of off-flavor compounds such as 2-methyl-isoborneol (2-MIB) and Geosmin (GSM). The regular purge and trap extraction followed by analysis with gas chromatography-mass spectrometry (GC-MS) usually involve a complicated assembly of facilities, more working space, long sample preparation time, and headspace solid-phase microextraction (SPME). In this work, a method with easier sample preparation, fewer and simplified facilities, and without SPME on GC-MS analysis is developed for the determination of 2-MIB and GSM in fish samples. Unlike previous methods, solvent extract from samples, QuEChERS-based cleanup, and solid-phase extraction for concentration are applied. The LOD (S/N > 3) and LOQ (S/N > 10) of this method were validated at 0.6 μg/kg and 1.0 μg/kg for both 2-MIB and GSM, which are under the sensory limit (1 μg/kg). Application of this method for incurred fish samples demonstrated acceptable analytical performance. This method is suitable for large-scale determination of 2-MIB and GSM in fish samples, owing to the use of simple facility and easy-to-operate procedure, rapid sample preparation, and shorter time for GC-MS analysis without SPME.

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