scholarly journals Immune complexes in blood serum of calves with clinical symptoms of bronchopneumonia

2011 ◽  
Vol 65 (1-2) ◽  
pp. 13-24
Author(s):  
Natalija Fratric ◽  
Dragan Gvozdic ◽  
Olivera Savic ◽  
Ivana Jaglicic ◽  
Vesna Ilic
Keyword(s):  
Immune Complexes ◽  
Immune Status ◽  
Clinical Symptoms ◽  
Electrophoretic Analysis ◽  
Circulating Immune Complexes ◽  
Infectious Agents ◽  
Diagnostic Parameter ◽  
Environment Management ◽  
Additional Diagnostic Parameter

Pneumonia in preruminant calves is a multifactorial disease. Infectious agents, the environment, management and the immune status of the calves are all important factors in determining the outcome of an infection. Until today, the level and composition of circulating immune complexes in preruminant calves with pneumonia have not been studied in detail. We performed this work with the aim to determine whether pneumonia in three-month-old calves is followed by changes in the immune complex level and changes in the ?-globulin level as their possible constituents. Immune complexes from the calves? sera were isolated by polyethylene glycol (PEG) precipitation methods. Optical density at 350 nm (OD350) of redissolved precipitates was measured to determine the circulating immune complexes level. The OD350 level of PEG precipitates of calves with pneumonia at the time of diagnosis was 0.577?0.206 and it was statistically significantly higher (p<0.001) than OD350 the level of PEG precipitates of healthy calves (0.286?0.080). Electrophoretic analysis of sera and PEG precipitates showed that both slow and fast ?-globulins are found among serum and immune-complexes' ?-globulins, but the concentration of fast ?-globulins was significantly lower in sera of diseased calves. The level of PEG precipitable immune complexes was not correlated with the concentration of serum and PEG precipitable g-globulins. The results of this study have shown that by relatively simple PEG precipitation assay it is possible to detect an increased level of circulating immune complexes in calves with pneumonia. This can be used as an additional diagnostic parameter for the detection and follow up of the disease.

Dynamics of immunologic parameters in elder children with atopic dermatitis

1999 ◽  
Vol 80 (4) ◽  
pp. 253-255
Author(s):  
В. А. Shamov ◽  
A. G. Shamova ◽  
T. G. Malanicheva
Keyword(s):  
Atopic Dermatitis ◽  
Immune Complexes ◽  
Immune Status ◽  
Circulating Immune Complexes ◽  
Natural Killers ◽  
Cd8 Cells ◽  
Immunologic Parameters

In children aged 10 to 17 with atopic dermatitis the following changes are revealed: the decrease of the content of CD3+-cells, CD8+-cells, natural killers, CH50, the increase of immunoregulator index indicators, CD19+-cells, igg, ige circulating immune complexes. More pronounced disorders of the immune status are found in boys and girls aged 13 to 17 than in children aged 10 to 12. After the treatment the favourable dynamics of the indicators of CD8+-cells, immunoregulator index, concentration of igg, ige and circulating immune complexes is found.

scholarly journals The effect of interferons-α and –γ and their combination with dimethyl dipyrazolyl selenide on the immune status and functional state of the reproductive system of cows in adverse ecological conditions

2019 ◽  
pp. 53-57
Author(s):  
A. G. Shakhov ◽  
I. T. Shaposhnikov ◽  
Yu. N. Brigadirov ◽  
V. N. Kotsarev ◽  
V. N. Skorikov ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Functional State ◽  
Reproductive System ◽  
Immune Complexes ◽  
Immune Status ◽  
Lysozyme Activity ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Ecological Conditions ◽  
Positive Effect

The results of studying the effect of interferons alpha and gamma and their combination with dimethyl dipyrazolyl selenide on the immune status and functional state of the reproductive system of cows in adverse ecological conditions are presented. The positive effect of the drugs, especially their combination, on erythropoiesis and the immune status of animals, which was manifested by an increase in the content of erythrocytes, hemoglobin, hematocrit, leukocytes, total immunoglobulins, serum bactericidal and lysozyme activity, absorptive function of phagocytes, relative amount of T lymphocytes, and a decrease in the level of circulating immune complexes was stated. An increase in the immune status of animals at the effect of immunocorrecting drugs was accompanied by a decrease in the pathology of calving and postpartum complications and favorably affected the functional state of their reproductive system. The cows which were introduced interferons and their combination with dimethyl dipyrazolyl selenide demonstrated a shorter period from calving to successful insemination, as well as the insemination index and fertility, compared with the animals of the control group, which were not introduced any drugs.

scholarly journals The characteristics of serum circulating immune complexes of patients with atopic asthma with different severity degree

2013 ◽  
Vol 94 (5) ◽  
pp. 744-748 ◽  
Author(s):  
Y V Skibo ◽  
N S Kurmaeva ◽  
V N Tsibulkina ◽  
I G Mustafin ◽  
Z I Abramova
Keyword(s):  
Severe Asthma ◽  
Bronchial Asthma ◽  
Immune Complexes ◽  
Wave Length ◽  
Molecular Complexes ◽  
Electrophoretic Analysis ◽  
Circulating Immune Complexes ◽  
Atopic Asthma ◽  
Control Group ◽  
Serum Samples

Aim. To evaluate the serum level of pathogenic circulating immune complexes in patients with mild and severe atopic bronchial asthma. Methods. Serum samples of patients with atopic asthma of mild persistent (30 patients) and severe persistent (20 patients) forms were analyzed. The control group consisted of 15 healthy volunteers. To detect the giant, large, medium and small-sized serum immune complexes, 3, 3.5, 4 and 7% polyethyleneglycol-6000 solutions were used. For quantitative evaluation of the immune complexes we measured the ultraviolet optical density at 280 nm wave length. To separate the immune complexes from immunoglobulin, Protein-G-Sepharose was used. Determination of the protein composition of circulating immune complexes was performed by electrophoresis in 8% polyacrylamide gel. Results. The concentration of immune complexes was increased in patients with bronchial asthma compared to healthy donors. Small and medium-sized immune complexes were prevailing, their concentrations correlated with the severity of asthma. Large, medium and small-sized immune complexes participated in immunopathological reactions in patients with both mild and severe asthma, with immune complexes pathogenicity coefficient significantly increased depending on the severity of the disease. Electrophoretic analysis of circulating immune complexes has shown the presence of proteins with molecular weight of 60 kDa in the complexes of all sizes. In the severe asthma group, an antigen fraction with a molecular mass of 36 kDa within the small-sized molecular complexes was revealed. Conclusion. The observed increase of small and medium-sized circulating immune complexes serum levels in patients with bronchial asthma may be an indicator of of these patients predisposal to autoimmune reactions development.

scholarly journals Immune related prognosis biomarkers for outcomes of joint preservation surgery in children with ischemic deformity of the proximal femur (preliminary results)

2020 ◽  
Vol 26 (4) ◽  
pp. 516-520
Author(s):  
M.P. Teplenky ◽  
◽  
M.V. Chepeleva ◽  
E.I. Kuznetsova ◽  
◽  
...  
Keyword(s):  
Proximal Femur ◽  
Immune Complexes ◽  
Immune Status ◽  
Circulating Immune Complexes ◽  
Immunological Parameters ◽  
Humoral Immune ◽  
Joint Preservation ◽  
Group I ◽  
Low Levels ◽  
Group Ii

Objective Assess the immune status of children with ischemic deformity of the proximal femur taking into account the treatment outcome. Material and methods Preoperative assessment of the immune status was performed for 16 adolescents with ischemic deformity of the proximal femur. Patients' age was 14.4 ± 0.45 years. Immunological parameters of 12 adolescents with satisfactory (group I) and 4 patients with poor (group II) anatomical and functional outcome of surgical treatment were compared. Peripheral blood lymphocyte typing was produced with laser cytometry assay, and IgA, IgM, IgG, IgE, IL-18, IFNγ and circulating immune complexes were quantified with enzyme-linked immunoassay. Results Сomparative analysis of preoperative immunological parameters showed statistically significant decline of Т-helper cells (СD3+СD4+), high levels of activated СD3+CD25 and СD3+HLADR T lymphocytes, low levels of activated NК cells (СD8dimCD38+) and IFNγ in group II. Humoral immune parameters indicated to low levels of IgA, IgM, IgG and higher levels of circulating immune complexes in group II relative to group I. Conclusion Preliminary data obtained suggest that cellular and humoral immune deficiency can be associated with unfavorable outcome of joint preservation procedure. Such immunological parameters as T lymphocytes subpopulation, immunoglobulins, circulating immune complexes and IFNγ can be used as significant prognostic factors for preoperative planning and postoperative rehabilitation of adolescents with ischemic deformity of the proximal femur.

Prognostic Significance of Circulating Immune Complexes in a Long-Term Follow-Up of Breast Cancer Patients

Oncology ◽  
1988 ◽  
Vol 45 (5) ◽  
pp. 337-343 ◽  
Author(s):  
Rosario Vincenzo laffaioli ◽  
Armando Bianchin ◽  
Giuseppina Ruggiero ◽  
Giuseppe Pirozzi ◽  
Achille Ungaro ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Immune Complexes ◽  
Prognostic Significance ◽  
Circulating Immune Complexes ◽  
Breast Cancer Patients ◽  
Long Term Follow Up

scholarly journals Immunological Indicators in Dogs Affected by Deep Pyoderma

2007 ◽  
Vol 76 (2) ◽  
pp. 283-290
Author(s):  
F. Špruček ◽  
M. Toman ◽  
M. Faldyna ◽  
M. Svoboda
Keyword(s):  
Immune Complexes ◽  
Clinical Symptoms ◽  
Young Age ◽  
Circulating Immune Complexes ◽  
Immunological Parameters ◽  
Immune State ◽  
The Relationship ◽  
Reduced Activity ◽  
Cd4 Lymphocytes

We examined the immune state of dogs with deep pyoderma to elucidate the relationship between clinical symptoms and immunological processes. We measured selected immunological parameters in control dogs (n = 10) and dogs of various breeds (German Shepherds were not included) affected by deep pyoderma (n = 25). We found that the affected dogs had increased relative counts of neutrophils, decreased relative and absolute counts of lymphocytes, higher relative counts of CD3+ lymphocytes and CD8+ lymphocytes but lower relative counts of CD21+ lymphocytes and CD4+ lymphocytes. The affected dogs exhibited a reduced activity of nonstimulated as well as mitogen-stimulated lymphocytes. Lower values of circulating immune complexes (CIC) in affected dogs were conditioned by the young age of animals. Nevertheless, the changes found in our study were not expressed in all animals.

scholarly journals Detection of circulating immune complexes in patients with hemorrhagic fever with renal syndrome

1985 ◽  
Vol 66 (4) ◽  
pp. 306-307
Author(s):  
I. N. Gavrilovskaya ◽  
E. A. Gorbachkova ◽  
M. A. Zakharova ◽  
S. B. Bogdanova ◽  
V. K. Podgorodnichenko
Keyword(s):  
Incubation Period ◽  
Immune Complexes ◽  
Clinical Symptoms ◽  
Hemorrhagic Fever ◽  
Circulating Immune Complexes ◽  
Renal Pathology

Severe renal pathology, a long incubation period (up to 3 weeks), a rise in the level of immunoglobulins in the first days from the onset of the disease, slowly developing clinical symptoms (within 2-3 weeks) suggest an important role of immune complexes in the pathogenesis of hemorrhagic fever with renal syndrome ( HFRS) in humans.

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