Presence of histopathological premalignant lesions and infection caused by high-risk genotypes of human papillomavirus in patients with suspicious cytological and colposcopy results: A prospective study

Background/Aim. In patients with premalignant cervical lesions, human papillomavirus (HPV) infection, at any moment, may be spontaneously eliminated, or may persist or transform cervical epithelium from a lower to a higher degree. Due to that, it is necessary to wisely select the patients who are at high risk of cancer development. The aim of the study was to establish the interdependence between a suspicious Papanicolaou (Pap) test and colposcopy with the infection caused by high-risk genotypes of human papillomavirus and the presence of premalignant cervical lesions. Methods. This prospective study used cytological, colposcopy, real-time polymerase chain reaction (PCR) of high-risk genotypes of human papillomavirus and histopathological analysis of cervical biopsy specimen. Out of 2,578 female patients sent to cytological analyses in Clinical Center of Montenegro, during 2012, 2013 and 2014, the study included 80 women who had to submit their biopsy specimens due to a suspicious Pap test and atypical colposcopy results. Results. In the group of 80 (3.1%; n = 80/2,578) of the selected female patients with suspicious Pap test and colposcopy, 2/3 or 56 (70%) of them had cervicitis, and 1/3 or 24 (30%) had cervical intraepithelial neoplasia. The most common type in cervical intraepithelial neoplasia was HPV16 in 8 female patients, ie 61.53% out of the number of infected, or 33.33% out of the total number of premalignant lesions. Conclusion. Patients with suspicious Papanicolaou test, colposcopy results and infection which is caused by high-risk HPV infection (HPV 16 in particular) often have premalignant cervical lesions. In these cases, histopathological confirmation of lesions is mandatory, since it serves as a definitive diagnostic procedure.

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Correlation between Human Papillomavirus Codetection Profiles and Cervical Intraepithelial Neoplasia in Japanese Women

Microorganisms ◽

10.3390/microorganisms8121863 ◽

2020 ◽

Vol 8 (12) ◽

pp. 1863

Author(s):

Kaori Okayama ◽

Hirokazu Kimura ◽

Koji Teruya ◽

Yasuyoshi Ishii ◽

Kiyotaka Fujita ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Cervical Intraepithelial Neoplasia ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Hpv Genotypes ◽

High Risk Type ◽

Pcr Method ◽

Polymerase Chain ◽

High Risk Hpv

Human papillomavirus (HPV) infection is thought to be strongly associated with the precarcinomatous state cervical intraepithelial neoplasia (CIN) and cervical carcinoma. To accurately assess the correlation between HPV detection profiles and CIN, the uniplex E6/E7 polymerase chain reaction (PCR) method was used. We detected HPV (37 genotypes) in 267 CIN cases. The detection of a single high-risk HPV genotype occurred in 69.7% of CIN1 and worse than CIN1 (CIN1+) cases whereas other types were detected in 11.6% of cases. Codetection of high-risk HPV genotypes occurred in 4.9% of CIN1+ cases. The high-risk genotype HPV16 was the most frequently detected genotype in CIN1+ lesions; the genotype HPV34 (not a high-risk type) was detected in some CIN3 cases. Furthermore, HPV codetection may not be associated with CIN grades. These results suggest that various HPV genotypes are associated with CIN across all analyzed cases.

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Clinical Utility of Adjunctive High-Risk Human Papillomavirus DNA Testing in Women With Papanicolaou Test Findings of Atypical Glandular Cells

Archives of Pathology & Laboratory Medicine ◽

10.5858/2008-0755-oar1.1 ◽

2010 ◽

Vol 134 (1) ◽

pp. 103-108

Author(s):

Chengquan Zhao ◽

Anca Florea ◽

R. Marshall Austin

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Cervical Intraepithelial Neoplasia ◽

Pap Test ◽

Intraepithelial Neoplasia ◽

Adenocarcinoma In Situ ◽

Test Results ◽

Pap Tests

Abstract Context. Atypical glandular cell (AGC) Papanicolaou (Pap) test interpretations are challenging. Most biopsy findings are benign, but AGC results may also reflect highly significant noninvasive neoplastic and malignant histologic outcomes. High-risk human papillomavirus (hrHPV) test use with AGC Pap test results is evolving. Objective. To further evaluate the utility and limitations of hrHPV testing with AGC Pap tests. Design. Hospital records were searched for AGC Pap tests results from June 1, 2005, to August 31, 2007. Cases of AGC with hrHPV tests and histopathologic follow-up were included. Results. Of the 662 women with AGC Pap test results and follow-up analyzed, hrHPV results were available for 309 (46.7%) and were positive in 75 cases (24.3%). Among the 75 cases with hrHPV+ AGC results, 13 (17.3%) had cervical intraepithelial neoplasia grades 2/3, 10 (13.3%) had adenocarcinoma in situ, and 3 (4.0%) had cervical invasive adenocarcinoma, whereas for 234 women with hrHPV− results, 1 (0.4%) had cervical intraepithelial neoplasia grades 2/3, 1 (0.4%) had adenocarcinoma in situ, 1 each (0.4%) had cervical adenocarcinoma and ovarian carcinoma, and 8 (3.4%) had endometrial carcinoma. Conclusions. Positive hrHPV AGC results were most strongly associated with cervical intraepithelial neoplasia grades 2/3 and adenocarcinoma in situ in women younger than 50 years. Positive hrHPV AGC results were also present in all 3 cases of invasive cervical adenocarcinoma in women younger than 50 years. Of note, hrHPV− AGC results were present in 10 of 13 carcinomas (76.9%) detected after AGC Pap tests, all in women 40 years or older with endometrial adenocarcinomas (n = 8), ovarian carcinoma (n = 1), and cervical adenosquamous carcinoma in a woman (n = 1) in her 50s. Testing for hrHPV after AGC Pap testing was most helpful in the detection of cervical intraepithelial neoplasia grades 2/3, adenocarcinoma in situ, and invasive cervical adenocarcinomas in women younger than 50 years.

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HPV Detection Methods

Disease Markers ◽

10.1155/2007/147429 ◽

2007 ◽

Vol 23 (4) ◽

pp. 273-281 ◽

Cited By ~ 46

Author(s):

Antoinette A. T. P. Brink ◽

Peter J. F. Snijders ◽

Chris J. L. M. Meijer

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

High Risk ◽

Cervical Intraepithelial Neoplasia ◽

Causal Relation ◽

Cervical Screening ◽

Intraepithelial Neoplasia ◽

Detection Methods ◽

High Grade ◽

Cervical Lesions

Given the causal relation between a persistent high-risk human papillomavirus (hrHPV) infection and the development of high-grade cervical intraepithelial neoplasia (CIN) and cervical cancer, hrHPV testing has been advocated in addition to cytology for the detection of clinically relevant cervical lesions. HrHPV testing is thought to improve cervical screening algorithms, the management of women with cytologically equivocal smears, and the management of women treated for high grade CIN. In this chapter we discuss different methods for HPV detection and genotyping and their respective applications.

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Associations of Cervicovaginal Lactobacilli With High-Risk Human Papillomavirus Infection, Cervical Intraepithelial Neoplasia, and Cancer: A Systematic Review and Meta-Analysis

The Journal of Infectious Diseases ◽

10.1093/infdis/jiz325 ◽

2019 ◽

Vol 220 (8) ◽

pp. 1243-1254 ◽

Cited By ~ 7

Author(s):

Huiyan Wang ◽

Yan Ma ◽

Ruonan Li ◽

Xixi Chen ◽

Lijuan Wan ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Cervical Intraepithelial Neoplasia ◽

Protective Factor ◽

Meta Analysis ◽

Intraepithelial Neoplasia ◽

Fixed Effect Model ◽

Hpv Infection ◽

Lactobacillus Species ◽

Lactobacillus Spp

Abstract Background Vaginal dysbiosis characterized by depleted lactobacilli is usually correlated with human papillomavirus (HPV) infection and cervical carcinogenesis, but the effect of the Lactobacillus genus and represented species on this process remains unclear. Methods PubMed, EMBASE, and CENTRAL databases were searched up to February 15, 2019. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using a fixed-effect model and Review Manager (version 5.3) for Mac. Results Eleven studies comprising 1230 cases were included. Lactobacillus spp. was associated with the decreased detection of high-risk subtype (hr)HPV infection (OR = 0.64, 95% CI = 0.48–0.87, I2 = 6%), cervical intraepithelial neoplasia (CIN) (OR = 0.53, 95% CI = 0.34–0.83, I2 = 0%), and cervical cancer (CC) (OR = 0.12, 95% CI = 0.04–0.36, I2 = 0%). At the level of Lactobacillus species, Lactobacillus crispatus, but not Lactobacillus iners, was correlated with the decreased detection of hrHPV infection (OR = 0.49, 95% CI = 0.31–0.79, I2 = 10%) and CIN (OR = 0.50, 95% CI = 0.29–0.88, I2 = 0%). Conclusions Cervicovaginal Lactobacillus spp. is associated with the decreased detection of hrHPV infection, CIN, and CC; L. crispatus may be the critical protective factor.

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The specific features of cervical intraepithelial neoplasia associated with persistent HPV infection

GYNECOLOGY ◽

10.26442/20795696.2019.3.190519 ◽

2019 ◽

Vol 21 (3) ◽

pp. 35-39

Author(s):

Tatiana V Klinyshkova ◽

Mariia S Buyan

Keyword(s):

Human Papillomavirus ◽

Prospective Study ◽

Cervical Intraepithelial Neoplasia ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Complex Treatment ◽

Human Papillomavirus Infection ◽

Papillomavirus Infection ◽

Laboratory Features ◽

Hpv Types

Aim. Of the study was to assess the clinical and laboratory features of cervical intraepithelial neoplasia (CIN) with persistent human papillomavirus infection and the development of prognostic criteria for persistence of HPV. Materials and methods. The prospective study included 63 patients with HPV-associated CIN. Assessment of persistence of HPV was based on detection of HPV when retesting 12 months. Depending on the results of retesting there were 2 groups: group 1A (main group, n=26), including patients with CIN and HPV persistence after treatment, mean age - 33.69±1.92 years; group 1B (comparison, n=37) - patients with CIN without HPV persistence after treatment, mean age - 34.43±2.09 years. Results. According to the results of the first HPV genotyping (before treatment) among patients of group 1A there was a predominance of two or more HPV types (34.6% vs 16.2% of patients of group 1B; p0.05). According to the results of the second genotyping (12 months after complex treatment) there was a 3.5-fold prevalence of patients with mono-infection against HPV co-infection in group 1A (p

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The Correlation Between Bacterial Vaginosis, High-Risk Human Papillomavirus Infection, and Cervical Intraepithelial Neoplasia Progression

10.21203/rs.3.rs-998392/v1 ◽

2021 ◽

Author(s):

Xiaolin Xu ◽

Yichan Zhang ◽

Liqun Yu ◽

Xingxian Shi ◽

Min Min ◽

...

Keyword(s):

Risk Factors ◽

Human Papillomavirus ◽

High Risk ◽

Bacterial Vaginosis ◽

Cervical Intraepithelial Neoplasia ◽

Protective Factor ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Vaginal Microbiota ◽

Papillomavirus Infection

Abstract Persistent infection with high-risk human papillomavirus (HR-HPV) is an important reason for the progression of cervical intraepithelial neoplasia (CIN). Bacterial vaginosis (BV) is a genital infection that frequently presents in women infected with HPV, but the correlation between BV and HPV during CIN development is still elusive. In this study, we enrolled 624 participants and obtained 423 samples of vaginal secretions from them, including 193 HPV-negative samples and 230 HPV-positive samples. We used 16S rRNA sequencing to measure the vaginal microbiota diversity in women with or without BV and HPV co-infection and then calculated risk factors for CIN progression by logistic regression. We found that condom use (OR=3.480; 95% CI=1.069-11.325; P < .05) was a protective factor against CIN, whereas BV (OR=0.358; 95% CI=0.195-0.656; P < .05) and HR-HPV infection (OR= 0.016; 95% CI=0.004-0.072; P < .001) were risk factors for CIN. BV and HPV infection could trigger an increase in the diversity of vaginal microbiota and decrease Lactobacillus domination, which is conducive to CIN regression. The depletion of the carbohydrate metabolism pathway may induce Lactobacillus reduction. Treating BV in the clinical setting could block CIN development and L. iners may be a crucial species during this process.

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The relationship between human papillomavirus infection and cervical intraepithelial neoplasia in Turkish women

International Journal of Gynecological Cancer ◽

10.1111/j.1525-1438.2007.00944.x ◽

2007 ◽

Vol 17 (6) ◽

pp. 1266-1270 ◽

Cited By ~ 25

Author(s):

M. M. Inal ◽

Ş. KÖSE ◽

Y. Yildirim ◽

Y. ÖZDEMIR ◽

E. TÖZ ◽

...

Keyword(s):

Human Papillomavirus ◽

Cervical Intraepithelial Neoplasia ◽

Pap Smear ◽

Intraepithelial Neoplasia ◽

Turkish Population ◽

Hpv Infection ◽

Pap Smears ◽

Turkish Women ◽

Cervical Lesions ◽

Chi Square

To compare the prevalence of cervical human papillomavirus (HPV) infection in Turkish women with normal Papanicolaou (Pap) smear and cervical intraepithelial neoplasia (CIN). In between March 2002 and November 2005, the study was designed as case–control study. Cytologic abnormalities in Pap smears were classified according to the Bethesda System (2001). Identification of the presence of HPV was carried out by the Hybrid Capture II test for all patients. To compare the groups, Chi-square test was used. A total of 1353 reproductive aged women were screened. Of them, 1344 (99.3%) had normal or class I Pap smear. Remaining nine cases (0.7%) had CIN at several degrees (five CIN I, three CIN II, and one carcinomain situ). While all these nine cases with cervical pathologies had HPV, only 20 cases from the other group (1.5%) had HPV (χ2466.1;P= 0.0001). This is the first study of the evaluation of the association between HPV and preinvasive cervical lesions in Turkish population. In spite of low general frequency (2.1%) of cervical HPV colonization in this population, a strong correlation was found between HPV and CIN.

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Molecular characterization of high-risk human papillomavirus (HR-HPV) in women in Lomé, Togo

BMC Infectious Diseases ◽

10.1186/s12879-021-05956-5 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Akouélé P. Kuassi-Kpede ◽

Essolakina Dolou ◽

Théodora M. Zohoncon ◽

Ina Marie Angèle Traore ◽

Gnatoulma Katawa ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

West Africa ◽

Public Health Problem ◽

Hpv Infection ◽

Cervical Lesions ◽

Hpv Genotypes ◽

Precancerous Cervical Lesions ◽

High Risk Hpv ◽

First Time

Abstract Background The causative agent of cervical cancer referred to as Human papillomavirus (HPV) remains a real public health problem. Many countries in West Africa, such as Togo have no data on the high-risk HPV (HR-HPV) infection and genotypes distribution. In order to fill the knowledge gap in the field in Togo, the main objective of this study was to determine the prevalence of pre-cancerous lesions of the cervix and HR-HPV genotypes among Togolese women. Methods Samples were collected from 240 women by introducing a swab in the cervix. Then, the screening of precancerous cervical lesions using the visual inspection with acetic acid and lugol (VIA / VIL) was conducted. The HR-HPV genotypes were characterised by real-time multiplex PCR. Results Out of 240 women recruited, 128 (53.3%) were infected by HR-HPV. The most common genotypes were HPV 56 (22.7%), followed by HPV 51 (20.3%), HPV 31 (19.5%), HPV 52 (18.8%) and HPV 35 (17.2%). The least common genotypes were HPV 33 (2.3%) and HPV 16 (2.3%). Among the women, 1.3% (3/240) were positive to VIA/VIL. Conclusion This study allowed HR-HPV genotypes to be characterised for the first time in Lomé, Togo. This will help in mapping the HR-HPV genotypes in West Africa.

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