scholarly journals Genetically modified soybean plants and their ecosystem

2004 ◽  
pp. 39-44
Author(s):  
Mirjana Milosevic ◽  
Marija Zlokolica ◽  
Petar Sekulic ◽  
Mirjana Jarak ◽  
Ksenija Taski
Keyword(s):  
Animal Feed ◽  
Genetically Modified ◽  
Genetically Modified Plants ◽  
Plant Genome ◽  
Genetically Modified Soybean ◽  
New Genes ◽  
Cultivated Plant ◽  
Polymerase Chain ◽  
Soybean Plants ◽  
Soil Microbiological Activity

Transgenic plants are developed by introgressing new genes using methods of molecular genetics and genetic engineering. The presence of these genes in plant genome is identified on the basis of specific oligonucleotides primers, and the use of PCR (Polymerase Chain Reaction) and DNA fragments multiplication. Genetically modified plants such as soybean constitute a newly created bioenergetic potential whose gene expression can cause disturbance of the biological balance ecosystem, soil structure and soil microbiological activity. Genetically modified plants may acquire monogenic or polygenic traits causing genetic and physiological changes in these plants, which may elicit a certain reaction of the environment including changes of microbiological composition of soil rhizosphere. The aim of introgressing genes for certain traits into a cultivated plant is to enhance its yield and intensify food production. There are more and more genetically modified plant species such as soybean, corn, potato, rice and others and there is a pressure to use them as human food and animal feed. Genetically modified soybean plants with introgressed gene for resistance to total herbicides, such as Round-up, are more productive than non-modified herbicide-sensitive soybeans.

Evaluation of polymerase chain reaction and DNA isolation protocols for detection of genetically modified soybean

2007 ◽  
Vol 42 (10) ◽  
pp. 1249-1255 ◽  
Author(s):  
Cibele dos Santos Ferrari ◽  
Luciana Lehmkuhl Valente ◽  
Fábio Cristiano Angonesi Brod ◽  
Caroline Tagliari ◽  
Ernani Sebastião Sant'Anna ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Isolation ◽  
Genetically Modified ◽  
Chain Reaction ◽  
Genetically Modified Soybean ◽  
Polymerase Chain

scholarly journals Risk Assessment of Genetically Modified Plants: A Review

2020 ◽  
pp. 1-10
Author(s):  
Hamza Armghan Noushahi ◽  
Mubashar Hussain
Keyword(s):  
Risk Assessment ◽  
Animal Feed ◽  
Genetically Modified ◽  
Genetically Engineered ◽  
Genetically Modified Plants ◽  
Human Consumption ◽  
Biotechnology Firms ◽  
Governmental Organizations ◽  
Non Governmental Organizations ◽  
Gm Plants

The health of genetically engineered foods/plants, which is one of the significant issues has been raised in recent years. Various non-governmental organizations and customers recommended that all GM foods before authorization for human consumption should be subject to long-term animal feed studies. The fundamental purpose of this review is to assess the new potential harmful impact/safety assessment of genetically engineered plants for the use of humans. A balance in the number of research groups, depending on their research, a variety of GM crops (maize and soybeans in particular) are varied as for traditional non-genetically modified plants. It is worth remembering that most of the experiments were carried out in biotechnology firms that sell these GM plants. In this review, we discussed in detail the risk assessment of genetically modified plants.

scholarly journals 082 Infection and Transformation of Rhododendron by Agrobacterium tumefaciens Strain B6

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 455C-455 ◽  
Author(s):  
R.R. Tripepi ◽  
M.W. George ◽  
T. Sripo ◽  
S.A. Johnsen ◽  
A.B. Caplan
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetically Modified ◽  
Green Fluorescence Protein ◽  
Plant Genome ◽  
Wild Type ◽  
Bacterial Strains ◽  
Green Fluorescence ◽  
Physical Presence ◽  
Fluorescence Protein ◽  
Polymerase Chain

The objective of this study was to determine if selected strains of Agrobacterium could infect microshoots of Rhododendron catawbiense. Fifteen microshoot stems of R. catawbiense var. album `America', `Joe Paterno', and `Cunningham's White' were inoculated with two drops (about 25 μL) of wild type Agrobacterium tumefaciens strains C58 or B6 or with wild type A. rhizogenes strain E8/73. Five control shoots were inoculated with 1.2 mM KH2PO4 buffer. Microshoots were grown on woody plant medium (WPM) supplemented with 4.9 μM 2iP. Six weeks after inoculation galls that formed were excised from the microshoots and placed on WPM that lacked plant growth regulators but contained 300 mg·L-1 cefotaxime. In another study, these wild-type bacterial strains were genetically modified by inserting the pBINm-gfp5-ER plasmid, which contained genes coding for NPTII and green fluorescence protein (GFP), into the bacteria. These modified strains were inoculated on 15 stems of the three rhododendron cultivars and one variety. Calluses that formed were excised, placed on basal WPM with cefotaxime, and allowed to proliferate. Wild type C58 induced galls to form on `Joe Paterno', R.c. album, and `Cunningham's White' stems, whereas wild type B6 caused galls to form only on the latter two types of rhododendron. Wild-type E8/73 failed to induce gall formation on the rhododendrons. Only genetically modified B6 caused galls to form on only `Cunningham's White' microshoots (seven of 15 inoculated stems). Three of these galls fluoresced green under ultraviolet light. Physical presence of the NPTII and GFP genes in the plant genome was determined by polymerase chain reaction. This study demonstrated that R. catawbiense is susceptible to Agrobacterium infection, and this plant can be genetically transformed.

scholarly journals Genetically Modified Soybean Lines Exhibit Less Transcriptomic Variation Compared to Natural Narieties

2020 ◽  
Author(s):  
Yan Long ◽  
Wentao Xu ◽  
Caiyue Liu ◽  
Mei Dong ◽  
Xinwu Pei ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Animal Feed ◽  
Genetically Modified ◽  
Safety Evaluation ◽  
Human Consumption ◽  
Unintended Effects ◽  
Genetically Modified Soybean ◽  
Kegg Pathways ◽  
Whole Seed ◽  
Gm Soybean

Abstract BackgroundGenetically modified (GM) soybeans provide a huge amount of food for human consumption and animal feed. However, the possibility of unexpected effects of transgenesis has increased food safety concerns. High-throughput sequencing profiling provides a powerful approach to directly evaluate unintended effects caused by foreign genes.ResultsIn this study, we performed transcriptomic analyses to evaluate differentially expressed genes (DEGs) in individual soybean tissues, including cotyledon (C), germ (G), hypocotyl (H), and radicle (R), instead of using the whole seed, from four GM and three non-GM soybean lines. A total of 3,351 DEGs were identified among the three non-GM soybean lines. When the GM lines were compared with their non-GM parents, 1,836 to 4,551 DEGs were identified. Furthermore, Gene Ontology (GO) analysis of the DEGs showed more abundant categories of GO items (199) among non-GM lines than between GM lines and the non-GM natural varieties (166). Results of Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that most KEGG pathways were the same for the two types of comparisons.ConclusionsThe study successfully employed RNA sequencing to assess the differences in gene expression among four tissues of seven soybean varieties, and the results suggest that transgenes do not induce massive transcriptomic alterations in transgenic soybeans compared with those that exist among natural varieties. This work thus provides important support for safety evaluation of genetically modified soybeans based on seed tissues.

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