The Effect of an Artificial Substrate on Damselfly Predation

1985 ◽  
Vol 4 (4) ◽  
pp. 219-222
Author(s):  
Joseph C. Coughlan ◽  
Fred W. Rabe ◽  
Flash Gibson
Keyword(s):  
Artificial Substrate

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

The Preparation of an Artificial Reagent for the One-Stage Factor VIII Assay

1970 ◽  
Vol 23 (02) ◽  
pp. 306-312 ◽  
Author(s):  
D Nyman
Keyword(s):  
Factor Viii ◽  
Artificial Substrate ◽  
One Stage ◽  
The One

SummaryA method for preparation of an artificial substrate for the one-stage factor VIII assay is described. Standardization of the test is given. The method is specific and reproducible.

In Situ Coupling of Catalytic Centers into Artificial Substrate Mesochannels as Super‐Active Metalloenzyme Mimics

Small ◽  
2021 ◽  
pp. 2101455
Author(s):  
Jian Yang ◽  
Ke Li ◽  
Chunzhong Li ◽  
Jinlou Gu
Keyword(s):  
Artificial Substrate

Infection of cucumber seedling roots by Pseudomonas amygdali pv. lachrymans following artificial substrate inoculation

2021 ◽  
Author(s):  
Shengping Zhang ◽  
Xianglong Meng ◽  
Ying Cheng ◽  
Yanan Wang ◽  
Tongle Hu ◽  
...  
Keyword(s):  
Artificial Substrate ◽  
Cucumber Seedling ◽  
Seedling Roots ◽  
Pseudomonas Amygdali

scholarly journals An artificial test substrate for evaluating electron microscopic immunocytochemical labeling reactions.

1987 ◽  
Vol 35 (8) ◽  
pp. 909-916 ◽  
Author(s):  
G D Gagne ◽  
M F Miller
Keyword(s):  
Electron Microscope ◽  
Hepatitis B ◽  
Horseradish Peroxidase ◽  
Polyclonal Antibodies ◽  
Hepatitis B Surface Antigen ◽  
Immunogold Labeling ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
Electron Microscopic ◽  
Immunocytochemical Studies

We describe an artificial substrate system for optimization of labeling parameters in electron microscope immunocytochemical studies. The system involves use of blocks of glutaraldehyde-polymerized BSA into which a desired antigen is incorporated by a simple soaking procedure. The resulting antigen-impregnated artificial substrate can then be fixed and embedded identically to a piece of tissue. The BSA substrate can also be dried and then sectioned for immunolabeling with or without chemical fixation and without exposing the antigen to dehydrating agents and embedding resins. The effects of various fixation and embedding procedures can thus be evaluated separately. Other parameters affecting immunocytochemical labeling, such as antibody and conjugate concentration, can also be evaluated. We used this system, along with immunogold labeling, to determine quantitatively the optimal fixation and embedding conditions for labeling of hepatitis B surface antigen (HbsAg), human IgG, and horseradish peroxidase. Using unfixed and unembedded HBsAg, we were able to detect antigen concentrations below 20 micrograms/ml. We have shown that it is not possible to label HBsAg within resin-embedded cells using conventional aldehyde fixation protocols and polyclonal antibodies.

scholarly journals Unusual Farnesyl Pyrophosphate Synthetase Reaction of an Artificial Substrate with Ni2+

1986 ◽  
Vol 15 (11) ◽  
pp. 1885-1888 ◽  
Author(s):  
Yuji Maki ◽  
Gotaro Watanabe ◽  
Akio Saito ◽  
Tanetoshi Koyama ◽  
Kyozo Ogura
Keyword(s):  
Artificial Substrate ◽  
Farnesyl Pyrophosphate

Assessment of the Possibility of Biological Recultivation Tail Deposits in the Yenisei North

2021 ◽  
Vol 25 (5) ◽  
pp. 39-45
Author(s):  
A.Kh. Sariev ◽  
N.Yu. Cherbakova ◽  
N.Yu. Terentyeva
Keyword(s):  
Heavy Metals ◽  
Soil Cover ◽  
Artificial Substrate ◽  
Fertilizer N ◽  
Drainage Layer ◽  
Average Productivity ◽  
Plant Soil ◽  
Disturbed Areas ◽  
Storage Facilities

The assessment of the possibility of creating a plant-soil cover on an artificially created seal-gravel drainage layer with a rocky bed (h=110¸150 cm), on the disturbed areas of the adjacent areas of tailings storage facilities. Research for 2017–2019 shows that when sowing recult-vants with sowing standards of 150–200 kg/ha and doses of fertilizer N60P60K60 and N120P60K60 on an artificial substrate it is possible to form meadow formations with a density of grass 10–12000 pc/m2 and average productivity of 21–24 c/ha. A plant-soil-blooded blood with a turf layer of 8–10 cm is created with the simultaneous overlap of heavy metals of man-made substrates and elimination of pulp sands dusting, which contributes to the improvement of the microclimate of the environment.

Rate assay of with 4-nitrophenyl as an artificial substrate

1996 ◽  
Vol 251 (1) ◽  
pp. 53-64 ◽  
Author(s):  
Hideto Shibata ◽  
Tatsuhiko Yagi
Keyword(s):  
Artificial Substrate

scholarly journals Effects of periphyton on monoculture of Puntius gonionotus

2018 ◽  
Vol 8 (2) ◽  
pp. 13-23
Author(s):  
MI Hoque ◽  
AKMF Rahman ◽  
MA Mansur ◽  
S Rahman
Keyword(s):  
Experimental Period ◽  
Artificial Substrate ◽  
Total Alkalinity ◽  
Cow Dung ◽  
Fish Production ◽  
Chemical Parameters ◽  
Physico Chemical ◽  
Triple Super Phosphate ◽  
Average Size ◽  
Experimental Ponds

An experiment was carried out on the effects of periphyton on monoculture of Thai sharputi, Puntius gonionotus at the Department of Fisheries Management, Bangladesh Agricultural University, Mymensingh during 7th August to 8th November. In treatment-1 bamboo poles were used as artificial substrate for periphyton production and in treatment-2 there was no artificial substrate (control). Each of the six ponds was stocked with 150 fingerlings of average size 6.41 cm and 3.60 g. The ponds were fertilized fortnightly with manure (cow dung) at a rate of 10 kg decimal-1, urea 60 g decimal-1 and triple super phosphate 90 g decimal-1. During the experimental period, the ranges of physico-chemical parameters viz. air temperature (31.0-35.50C), water temperature (29-320C), water depth (0.56-0.84 m), transparency (32-63 cm), dissolved oxygen (3.5-7.8 mg L-1), pH (6.8-7.9), total alkalinity (44-92 mg L-1), free CO2 (1.5-4.0 mg L-1), phosphate-phosphorus (0.31-1.07 mg L-1) and nitrate-nitrogen (1.12-2.30 mg L-1) were within the productive range and more or less similar in the ponds under treatments-1 and 2. Among the observed biological parameters, there were 35 genera of phytoplankton composed of five groups and 13 genera of zooplankton composed of four groups in the experimental ponds. Thirty three genera under the groups of Bacillariophyceae, Chlorophyceae, Cyanophyceae and Euglenophyceae formed the periphyton on bamboo poles in the experimental ponds. Net fish production of the ponds with periphyton under treatment-1 was about 1.5 times higher than those of the ponds without periphyton (treatment-2). By analysis of variance, it was found that the net fish production of Thai sharputi under treatment-1 was significantly higher than that under treatment-2 (p< 0.05). Finally, it can be concluded that periphyton is one of the preferable food item of Thai sharputi and it is also suggested that growth and production of Thai sharputi can be increased if arrangement is made for periphyton production. Int. J. Agril. Res. Innov. & Tech. 8 (2): 13-23, December, 2018

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