scholarly journals Combined effect of photobiomodulation with a matrix metalloproteinase inhibitor on the rate of relapse in rats

2015 ◽  
Vol 86 (2) ◽  
pp. 206-213 ◽  
Author(s):  
Sang-Hyun Lee ◽  
Kyung-A Kim ◽  
Stephanie Anderson ◽  
Yoon-Goo Kang ◽  
Su-Jung Kim
Keyword(s):  
Matrix Metalloproteinase ◽  
Relapse Rate ◽  
Combined Effect ◽  
Low Level Laser Therapy ◽  
Control Group ◽  
Protein Levels ◽  
Mmp Inhibitor ◽  
Irradiation Group ◽  
Level Laser ◽  
Relapse Rates

ABSTRACT Objective:  To investigate combined effect of photobiomodulation with a matrix metalloproteinase (MMP) inhibitor on the relapse rate in relation to MMP expression in rats. Materials and Methods:  Fifty-two rats were divided into four groups according to the treatment modality: control group, irradiation group, doxycycline group, and irradiation with doxycycline group. During a relapse period of 5 days after orthodontic movement, maxillary central incisors were treated by low-level laser therapy (LLLT) as a photobiomodulation and/or doxycycline as a synthetic MMP inhibitor. Relapse rate was evaluated in association with MMP expression at the gene and protein levels. Results:  Relapse rates were increased by LLLT (1.57-fold) and decreased by doxycycline (0.83-fold) compared with the control, showing positive correlation with the levels of expression for all MMPs in the periodontal ligament (PDL). LLLT concomitant with doxycycline administration resulted in no significant differences of relapse rate and MMP expression from the control. Conclusions:  The combined effect of photobiomodulation with an MMP inhibitor around the relapsing teeth proved to be antagonistic to PDL remodeling activity during relapse. This study suggests a basis for developing a novel biologic procedure targeting the MMP-dependent PDL remodeling to control the relapse rate.

Assessment of healing of dental implant surgical site following Low-Level Laser Therapy using Bio-Clinical parameters: An Exploratory Study

2020 ◽  
Author(s):  
VARSHA PALLED ◽  
DR. JITENDRA RAO ◽  
DR. RAGHUWAR DAYAL SINGH ◽  
DR. SHUCHI TRIPATHI ◽  
DR. KALPANA SINGH ◽  
...  
Keyword(s):  
Laser Therapy ◽  
Test Group ◽  
Low Level Laser Therapy ◽  
Control Group ◽  
Clinical Parameters ◽  
Implant Stability ◽  
Low Level ◽  
Implant Stability Quotient ◽  
Low Level Laser ◽  
Level Laser

The purpose of this study was to evaluate whether Low-Level Laser Therapy (LLLT) improves the healing of the implant surgical site with clinical and biochemical parameters.Thirty patients with an edentulous space spanning a single tooth were selected. The patients were randomly allocated to two groups - the control group and the test group. The test group received laser energy at a power of 2J/cm 2 with a total of 4-6J energy over each implant. Clinical parameters (Implant Stability Quotient, probing index, modified sulcus bleeding index)and osteoprotegerin (OPG) were assessed at baseline and follow-up intervals (2 weeks, 6 weeks, and 3 months).The test group showed significantly higher implant stability quotient compared to thecontrol group at 2 weeks(57.93±3.95 and 35.67±3.08; p<0.01) and 3months(58.86±3.75 and 67.06±3.78; p<0.01). A significant rise in OPG levels of the test group(686.30±125.36pg/ml at baseline and 784.25±108.30pg/ml at 3months;p<0.01) was seen contrary to significant decline in the control group (839.50±249.08pg/ml at baseline,415.30±78.39pg/ml at 3months;p<0.01). Within the limitations of the study, the study suggests that the healing of peri-implant hard and soft tissues may be enhanced with the use of LLLT as an explicit modality during the post-operative period.

scholarly journals Effect of low-level laser (Ga-Al-As 655 nm) on skeletal muscle fatigue induced by electrical stimulation in rats

2006 ◽  
Vol 101 (1) ◽  
pp. 283-288 ◽  
Author(s):  
Rodrigo Álvaro B. Lopes-Martins ◽  
Rodrigo Labat Marcos ◽  
Patrícia Sardinha Leonardo ◽  
Antônio Carlos Prianti ◽  
Marcelo Nicolas Muscará ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Electrical Stimulation ◽  
Muscle Contractions ◽  
Muscular Fatigue ◽  
Low Level Laser Therapy ◽  
Control Group ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Significant Difference

We investigated whether low-level laser therapy (LLLT) can reduce muscular fatigue during tetanic contractions in rats. Thirty-two male Wistar rats were divided into four groups receiving either one of three different LLLT doses (0.5, 1.0, and 2.5 J/cm2) or a no-treatment control group. Electrical stimulation was used to induce six tetanic muscle contractions in the tibial anterior muscle. Contractions were stopped when the muscle force fell to 50% of the initial value for each contraction (T50%). There was no significant difference between the 2.5 J/cm2 laser-irradiated group and the control group in mean T50% values. Laser-irradiated groups (0.5 and 1.0 J/cm2) had significantly longer T50% values than the control group. The relative peak force for the sixth contraction in the laser-irradiated groups were significantly higher at 92.2% (SD 12.6) for 0.5 J/cm2, 83.2% (SD 20.5) for 1.0 J/cm2, and 82.9% (SD 18.3) for 2.5 J/cm2 than for the control group [50% (SD 15)]. Laser groups receiving 0.5 and 1.0 J/cm2 showed significant increases in mean performed work compared with both the control group and their first contraction values. Muscle damage was indirectly measured by creatine kinase levels in plasma. A distinct dose-response pattern was found in which 1.0 and 2.5 J/cm2 LLLT groups had significantly lower creatine kinase levels than the 0.5 J/cm2 LLLT group and the control group. We conclude that LLLT doses of 0.5 and 1.0 J/cm2 can prevent development of muscular fatigue in rats during repeated tetanic contractions.

scholarly journals Orthokeratology and Low-Intensity Laser Therapy for Slowing the Progression of Myopia in Children

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Fen Xiong ◽  
Tian Mao ◽  
Hongfei Liao ◽  
Xiaoqin Hu ◽  
Lei Shang ◽  
...  
Keyword(s):  
Laser Therapy ◽  
Low Level Laser Therapy ◽  
Control Group ◽  
Myopia Progression ◽  
Level Laser ◽  
Spherical Equivalent Refraction ◽  
Treatment Changes ◽  
Low Intensity Laser Therapy ◽  
Intensity Laser ◽  
Myopia Control

Orthokeratology (OK) is widely used to slow the progression of myopia. Low-level laser therapy (LLLT) provides sufficient low energy to change the cellular function. This research is aimed at verifying the hypothesis that LLLT treatment could control myopia progression and comparing the abilities of OK lenses and LLLT to control the refractive error of myopia. Eighty-one children (81 eyes) who wore OK lenses, 74 children (74 eyes) who underwent LLLT treatment, and 74 children (74 eyes) who wore single-vision distance spectacles for 6 months were included. Changes in axial length (AL) were 0.23 ± 0.06   mm for children wearing spectacles, 0.06 ± 0.15   mm for children wearing OK lens, and − 0.06 ± 0.15   mm for children treated with LLLT for 6 months. Changes in subfoveal choroidal thickness (SFChT) observed at the 6-month examination were − 16.84 ± 7.85   μ m , 14.98 ± 22.50   μ m , and 35.30 ± 31.75   μ m for the control group, OK group, and LLLT group, respectively. Increases in AL at 1 month and 6 months were significantly associated with age at LLLT treatment. Changes in AL were significantly correlated with the baseline spherical equivalent refraction (SER) and baseline AL in the OK and LLLT groups. Increases in SFChT at 1 month and 6 months were positively associated with age at enrolment for children wearing OK lens. At 6 months, axial elongation had decelerated in OK lens-wearers and LLLT-treated children. Slightly better myopia control was observed with LLLT treatment than with overnight OK lens-wearing. Evaluations of age, SER, and AL can enhance screening for high-risk myopia, improve the myopia prognosis, and help determine suitable control methods yielding the most benefits.

scholarly journals A Randomized Comparative Study between High-Intensity and Low-Level Laser Therapy in the Treatment of Chronic Nonspecific Low Back Pain

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Walid Kamal Abdelbasset ◽  
Gopal Nambi ◽  
Saud F. Alsubaie ◽  
Ahmed M. Abodonya ◽  
Ayman K. Saleh ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Low Back Pain ◽  
Back Pain ◽  
Laser Therapy ◽  
High Intensity ◽  
Low Level Laser Therapy ◽  
Control Group ◽  
Low Back ◽  
Level Laser

Objectives. Chronic nonspecific low back pain (chronic nsLBP) is one of the most common musculoskeletal disorders leading to disabilities and physical inactivity. Laser therapy was used in chronic nsLBP treatment; however, no previous studies have assessed the impacts of high-intensity laser therapy (HILT) versus low-level laser therapy (LLLT) on chronic nsLBP. This study compared the effects of HILT versus LLLT on individuals suffering from chronic nsLBP. Methods. The study was a randomized control trial. Sixty individuals with chronic nsLBP were enrolled in this study between May and November 2019. All participants were clinically diagnosed with chronic nsLBP. They were assigned randomly into three groups, 20 in each group. The first group received a program of LLLT, the second group received a program of HILT, and the third did not receive laser therapy (control group). Pain severity, disability, lumbar mobility, and quality of life were assessed before and after 12-week intervention. Results. Both LLLT and HILT groups showed a significant improvement of the Oswestry Disability Index (ODI), visual analogue scale (VAS), lumbar range of motion (ROM), and European Quality of Life (EuroQol) scores ( p > 0.05 ), while the control group did not show significant changes ( p > 0.05 ). Comparison among the three study groups postintervention showed significant differences in the outcome measures ( p > 0.05 ), while comparison between the LLLT and HILT groups showed nonsignificant differences ( p > 0.05 ). Conclusion. There are no different influences of LLLT versus HILT on chronic nsLBP patients. Both LLLT and HILT reduce pain and disability and improve lumbar mobility and quality of life in chronic nsLBP patients.

ASSESSMENT ON RESULTS OF SURGICAL EXTRACTION OF  IMPACTED LOWER THIRD MOLARS WITH POSTOPERATIVE LOW - LEVEL LASER THERAPY

2018 ◽  
Vol 8 (6) ◽  
pp. 130-137
Author(s):  
Huong Nguyen Thi Mai ◽  
Tai Tran Tan ◽  
Khanh Hong Quoc
Keyword(s):  
Laser Therapy ◽  
Third Molar ◽  
Low Level Laser Therapy ◽  
Control Group ◽  
Low Level ◽  
Impacted Third Molar ◽  
Low Level Laser ◽  
Level Laser ◽  
Surgical Extraction ◽  
Wisdom Teeth

Background: The most frequently performed surgical procedure in dentistry is impacted third molar extraction with difficulty varies according to the location of the tooth. Laser therapy after surgery can accelerate cell and tissue reconstruction along with relieve post-operative pain. The objective of this study was to investigate the clinical and radiographic characteristics of impacted lower third molar and to evaluate the results of surgical extraction of impacted lower third molar with post-surgical low-level laser therapy (LLLT). Subjects and Methods: Clinical and radiographic data from 90 patients (average age 28.13 ± 5.38) subjected to a surgical extraction of lower third molar were pooled and divided randomly into 3 groups: group 1 received LLLT immediately after surgery intraorally, group 2 treated with LLLT immediately after the extraction extraorally. Patients received routine management with nonactivated laser were inserted in the control group. Assessments of pain, swelling and trismus level were carried out at 24, 48 hours and on the 7th day after surgery. Results: Correlation of wisdom teeth to ramus and adjacent teeth mainly type II (88.9%), type III accounted for 11.1%. Relative depth of wisdom teeth in the bone mainly position B (81.1%), position C (18.9%). Correlation of wisdom teeth axis to adjacent teeth: horizontal (58.9%), mesioangular impactions (40%) and distoangular impaction (1.1%). There were statistically significant decreases in the level of pain, edema and interincisal opening between the laser-treated groups and the control group on the 1st and 2nd postoperative day. Conclusions: Intraoral and extraoral post-surgical low-level laser therapy treatment was useful in reducing swelling, pain and trismus compared to placebo group in impacted third molar surgery. Key words: Impacted lower third molar, low- level laser therapy

scholarly journals Application of a Low-Level Laser Therapy and the Purified Protein from Natural Latex (Hevea brasiliensis) in the Controlled Crush Injury of the Sciatic Nerve of Rats: A Morphological, Quantitative, and Ultrastructural Study

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Fernando José Dias ◽  
João Paulo Mardegan Issa ◽  
Mamie Mizusaki Iyomasa ◽  
Joaquim Coutinho-Netto ◽  
Ricardo Alexandre Junqueria Calzzani ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Laser Therapy ◽  
Ultrastructural Analysis ◽  
Crush Injury ◽  
Low Level Laser Therapy ◽  
Control Group ◽  
Low Level ◽  
Injured Nerve ◽  
Low Level Laser ◽  
Level Laser

This study analyzed the effects of a low-level laser therapy (LLLT, 15 J/cm2, 780 nm wavelength) and the natural latex protein (P1, 0.1%) in sciatic nerve after crush injury (15 Kgf, axonotmesis) in rats. Sixty rats (male, 250 g) were allocated into the 6 groups (n=10): CG—control group; EG—nerve exposed; IG—injured nerve without treatment; LG—crushed nerve treated with LLLT; PG—injured nerve treated with P1; and LPG—injured nerve treated with LLLT and P1. After 4 or 8 weeks, the nerve samples were processed for morphological, histological quantification and ultrastructural analysis. After 4 weeks, the myelin density and morphological characteristics improved in groups LG, PG, and LPG compared to IG. After 8 weeks, PG, and LPG were similar to CG and the capillary density was higher in the LG, PG, and LPG. In the ultrastructural analysis the PG and LPG had characteristics that were similar to the CG. The application of LLLT and/or P1 improved the recovery from the nerve crush injury, and in the long term, the P1 protein was the better treatment used, since only the application of LLLT has not reached the same results, and these treatments applied together did not potentiate the recovery.

Equine wound healing: influence of low level laser therapy on an equine metacarpal wound healing model

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Henry W. Jann ◽  
Kenneth Bartels ◽  
Jerry W. Ritchey ◽  
Mark Payton ◽  
John M. Bennett
Keyword(s):  
Wound Healing ◽  
Laser Therapy ◽  
Low Level Laser Therapy ◽  
Energy Output ◽  
Control Group ◽  
Skin Wounds ◽  
Full Thickness ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

AbstractTo evaluate the effects of low level laser therapy (LLLT) on healing of full thickness symmetrical skin wounds in horses. LLLT is a therapeutic modality using the application of light, usually a low power laser or light emitting diode in the power range of 1 mW to 12 W that, in practical terms, promotes tissue regeneration as well as reducing inflammation and pain.Experimental study.Healthy horses (n=8).Full thickness, 2.5 cm square skin wounds were created in the mid-metacarpal region on one leg of eight normal horses. LLLT was used on limbs assigned to the experimental group and limbs assigned to the control group were allowed to heal without treatment. LLLT was administered using a line generated optical scanner with a dual diode laser system (model EML; Erchonia Laser Healthcare, McKinney, TX, USA) at a wavelength of 635 nm and an energy output of 17 mW per diode. Wound size was measured for an 80-day period post operatively. Eighty days after surgery incisional biopsies were examined histologically.Wounds treated with LLLT healed faster than the control wounds (LLLT increased the rate of wound healing.

scholarly journals Laser Management of Peri-Implantitis: A Comparison between Photodynamic Therapy Combined with Hydrogen Peroxide (OHLLT) and OHLLT + Er:YAG Laser. A Retrospective Controlled Study

2021 ◽  
Vol 11 (15) ◽  
pp. 6771
Author(s):  
Gianluigi Caccianiga ◽  
Gérard Rey ◽  
Paolo Caccianiga ◽  
Alessandro Leonida ◽  
Marco Baldoni ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Photodynamic Therapy ◽  
Bone Loss ◽  
Laser Therapy ◽  
Er:Yag Laser ◽  
Test Group ◽  
Low Level Laser Therapy ◽  
Controlled Study ◽  
Control Group ◽  
Level Laser

In peri-implantitis, treatment lasers are effectively used to enhance implant surfaces’ decontamination of bacteria. The type of lasers commonly used and tested in dentistry are Nd:YAG, Er:YAG and diodes. The Er:YAG laser is considered to be effective in removing biofilm from implant surfaces. Photodynamic therapy (PDT), realized with low-level laser therapy (LLLT) and a photosensitizer, seems to have limited efficacy in peri-implantitis management, while diode lasers used with high frequency and power (Wiser, Doctor Smile), performed with hydrogen peroxide 10 vol. 3% (OHLLT), seems to lead to a deep sanitization of implant surfaces, without any thermal effect. The aim of our retrospective controlled study is to compare an oxygen high-level laser therapy (OHLLT) protocol with OHLLT with an Er:YAG laser (Pluser, Doctor Smile) in order to maximize bacterial removal and to evaluate if an Er:YAG laser could improve hard tissue regeneration with respect to OHLLT alone. Two hundred and ten implants affected by peri-implantitis were divided into two groups: 88 implants were treated with OHLLT alone (control group) while 122 underwent OHLLT and Er:YAG treatment (test group). Mean bone loss before (T0) and after treatment (T1) (with a follow-up of 5 years) was registered for all implants. The collected data were analyzed with the Statistical Package for Social Sciences (SPSS) version 11.5. No statistically significant differences were found. In the OHLLT group, we noticed a mean bone loss after treatment of 2.1 mm in the upper arch and 2.4 mm in the mandible. In the OHLLT + Er:YAG group, the results indicate a mean bone loss of 2.0 mm in the upper arch and 2.5 mm in the mandible. Both groups showed a mean bone loss after treatment of 2.3 mm. According to the Kolmogorov–Smirnov test, overall, data followed a normal distribution (value of the K-S test statistic = 0.0912; p = 0.36112). OHLLT plays a central role in enhancing clinical results in peri-implantitis treatment, leading to an effective bacterial decontamination. Our results showed no statistically significant differences between OHLLT and OHLLT + Er:YAG laser; thus, the potential advantageous role of Er:YAG has been downgraded with the analysis of our study.

Low-level laser therapy on the rat’s gastrocnemius morphometry submitted to a rheumatoid arthritis model

2021 ◽  
Vol 19 (2) ◽  
pp. 111-115
Author(s):  
Estéfani Marin ◽  
◽  
Jacqueline Lumy Fuse ◽  
Larissa Pereira Lopes ◽  
Morgana Neves ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Laser Therapy ◽  
Wistar Rats ◽  
Low Level Laser Therapy ◽  
Lesion Group ◽  
Control Group ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Significant Difference

Introduction. Rheumatoid arthritis (RA) is a chronic, systemic, autoimmune inflammatory disease of unknown origin, mainly affecting synovial joints and related structures, including the adjacent musculature, generating great disability and reduction in quality of life. Aim. This study was designed to investigate the effect of low-level laser therapy (LLLT) on gastrocnemius of Wistar rats subjected to an experimental model of RA. Material and methods. Forty male Wistar rats were used, separated into: acute and chronic, being subdivided into Control Group (CG): without intervention, Lesion Group (LG): submitted to lesion, Laser Control Group (LCG): without lesion and with treatment, and Laser Lesion Group (LLG): submitted to lesion and LLLT. The treatment with LLLT occurred in four points of the right knee, wavelength of 660 nm, energy density of 5 J/cm2, energy per point of 0.003 J. Morphometric analysis was performed using a 40x magnification photomicrograph and analyzed using the Image-Pro-Plus 6.0 program. Results. As result of the acute group there was a difference only for muscle mass, being higher in CG. For the chronic group there was significant difference for cross-sectional area, larger and smaller diameter, again with the control group obtaining higher values than the others, for the number of nuclei LG was lower than CG and LCG, but LLG was not different from any of them. Conclusion. It is concluded that treatment with LLLT was not very effective in reversing the harmful effects of RA on the gastrocnemius muscle.

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