4 Cases Study of IgA Vasculitis That Serum D-dimer Level is Correlated with Disease Activity

Aims: To evaluate D-Dimer serum levels in patients with chronic urticaria and its correlation with disease activity. Settings and Design: Single centre Cross sectional prospective observational age & sex matched case-control study at Dermatology OPD of a tertiary referral centre. Methods and Material: This study was conducted from January 2018 to June 2019. We in-cluded 33 patients with CU and 30 controls . They were recruited from urticaria clinic. All cases were subjected to history taking, general and dermatological examination. The serum levels of D-Dimer were measured by Semiquantitative, immunofiltration kits. Statistical analysis: Data was analysed by Statistical Package for Social Sciences (SPSS) ver-sion 21.0. Tests used were Independent t test/Mann-Whitney Test, Chi-Square test/Fisher’s Exact test, Spearman rank correlation coefficient, Kolmogorov- Smirnov test.. Results: Patients with active CU had elevated D-Dimer serum levels (p<0.0001) when com-pared with the control group (papulo-squamous disorder). Of 33 CSU patients, D-dimer level was elevated in 19 patients (57.58%). There was statistically significant positive correlation between disease severity (UAS7) and plasma D-dimer level (p <.0001, r =0.935). Conclusions: This study showed elevated D-dimer levels in more than half of Indian patients with CSU. There was a positive correlation between plasma D-dimer levels and the severity of disease activity. Investigation for plasma D-dimer level may be an alternative objective way to evaluate disease severity in patients with CSU. Limitations: Low sample size . Semi quantitative method was used instead of ELISA for D-Dimer.

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P40 S100A8/A9 and S100A12 may be biomarkers for juvenile-onset systemic lupus erythematosus

Rheumatology ◽

10.1093/rheumatology/kez416.007 ◽

2019 ◽

Vol 58 (Supplement_4) ◽

Author(s):

Sean Joseph Donohue ◽

Angela Midgley ◽

Ian Bruce ◽

Michael W Beresford ◽

Christian M Hedrich

Keyword(s):

Disease Activity ◽

Conflicts Of Interest ◽

Renal Involvement ◽

S100 Protein ◽

S100 Proteins ◽

Healthy Controls ◽

Iga Vasculitis ◽

Juvenile Onset ◽

Global Disease Activity ◽

Serum And Urine

Abstract Background Juvenile-onset SLE (JSLE) is a systemic autoimmune disease affecting young people before the age of 17 years. Despite recent progress in understanding the pathogenesis of JSLE, it remains unclear. The lack of reliable biomarkers makes the assessment of disease activity and organ involvement challenging. A family of pro-inflammatory molecules known as S100 proteins have recently emerged as potential biomarkers for monitoring disease activity in an adult-onset SLE and various (auto-) inflammatory conditions. We investigated the usefulness of S100A8/A9 and S100A12 serum and urine levels as biomarkers for renal involvement and/or disease activity in JSLE. Methods Serum and urine from JSLE patients (n = 60), matched healthy controls (n = 53) and patients with IgA vasculitis (disease controls, n = 9) were collected. S100A8/A9 and S100A12 concentrations in serum and urine samples were quantified using the Meso Scale Discovery Immunoassay [serum; median ng/mL (I.Q.R), urine; median ng/mmol creatinine (I.Q.R)]. Furthermore, S100 protein concentrations in serum and urine were compared between groups and between JSLE patients with active (BILAG scores A, B, C) and inactive (BILAG scores D, E) renal disease. Potential effects of age, sex and global disease activity (SLEDAI scores) on S100 protein concentrations were tested. Results S100A8/A9 levels were significantly increased in the serum of JSLE patients [1577.80ng/mL (2313.97)] when compared to healthy controls [732.53 ng/mL (1003.47); p < 0.001)] and patients with IgA vasculitis [531.23 ng/mL (824.50); p < 0.05)]. This, however, was not reflected in patients’ urine samples. S100A12 concentrations were significantly increased in both the serum [41.12 ng/mL = (106.06); p < 0.05] and urine [90.12ng/mmol creatinine (348.44); p < 0.05] when compared to healthy controls [serum;17.75ng/mL(47.16), urine; 14.88ng/mmol creatinine (198.36)]. No differences were seen between S100A12 protein levels in serum or urine of JSLE patients when compared to patients with IgA vasculitis. No differences were observed between S100 protein concentrations in the serum or urine of patients with JSLE with active vs. inactive renal disease, between ages or sexes. Also, global disease activity (SLEDAI) did not correlate with serum or urine S100 protein concentrations. Conclusion JSLE patients exhibit increased concentrations of S100A8/A9 and S100A12 proteins in the serum, independent of clinical disease activity. Furthermore, increased urine concentrations of S100A12 in JSLE patients are independent of current renal involvement or activity. Based on the small molecular weight of S100 proteins, this may suggest renal loss of elevated serum S100A12 (21 kDa) through the kidneys rather than being the result of kidney inflammation or damage. Thus, S100 proteins may help to distinguish JSLE patients from healthy controls and patients with the differential diagnosis IgA vasculitis, supporting previous reports in adult-onset SLE. Longitudinal assessment of S100 proteins for an individual patient and/or in combination with other biomarkers may be helpful in assessing disease activity in JSLE patients. Conflicts of Interest The authors declare no conflicts of interest.

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Baseline D‐dimer plasma levels correlate with disease activity but not with the response to omalizumab in chronic spontaneous urticaria

Allergy ◽

10.1111/all.13936 ◽

2019 ◽

Vol 74 (12) ◽

pp. 2538-2538 ◽

Cited By ~ 2

Author(s):

Riccardo Asero ◽

Angelo V. Marzano ◽

Silvia Ferrucci ◽

Giovanni Genovese ◽

Massimo Cugno

Keyword(s):

Disease Activity ◽

Plasma Levels ◽

Chronic Spontaneous Urticaria ◽

D Dimer

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Clinical significance of blood platelets and mean platelet volume in patients with ulcerative colitis

Journal of International Medical Research ◽

10.1177/03000605211009715 ◽

2021 ◽

Vol 49 (4) ◽

pp. 030006052110097

Author(s):

Zhitao Chen ◽

Yaqi Lu ◽

Jie Wu ◽

Heng Zhang

Keyword(s):

Ulcerative Colitis ◽

Disease Activity ◽

Clinical Significance ◽

Mean Platelet Volume ◽

Blood Platelets ◽

Large Cell ◽

Platelet Volume ◽

The Difference ◽

Extent Of Disease ◽

D Dimer

Objectives This retrospective study aimed to investigate and analyze the clinical significance of blood platelets (PLTs) and mean platelet volume (MPV) in patients with ulcerative colitis (UC). Methods A total of 132 patients with UC and 208 healthy subjects were enrolled. PLTs, MPV, platelet-large cell rate (P-LCR), C-reactive protein (CRP), fibrinogen (FIB) and D-dimer were analyzed. Correlations were calculated between disease activity/extent of disease and MPV, PLT, and CRP levels in patients with UC. Results PLT levels were significantly higher while MPV and P-LCR were lower in patients with UC compared with controls. Disease activity was positive correlated with CRP (r = 0.564) and PLT (r = 0.307) but negatively correlated with MPV (r = −0.351). Extent of disease was positively correlated with CRP (r = 0.312) but showed no correlation with PLTs and MPV. FIB and D-dimer were higher in patients with UC, but the difference in FIB levels was not statistically significant. Conclusions PLTs and MPV are potential biomarkers for UC disease activity. These data may help clinicians to attain a more comprehensive judgment of the general condition of patients with UC.

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D-Dimer levels are correlated with disease activity in Crohn's patients

Oncotarget ◽

10.18632/oncotarget.19250 ◽

2017 ◽

Vol 8 (38) ◽

pp. 63971-63977 ◽

Cited By ~ 2

Author(s):

Junwu Zhang ◽

Zhen Guo ◽

Wei Yang ◽

Zhongliang Zhu ◽

Wanzhong Kong ◽

...

Keyword(s):

Disease Activity ◽

D Dimer

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Plasma Fibrinogen, D-dimer, and Fibrin Degradation Product as Biomarkers of Rheumatoid Arthritis

10.21203/rs.3.rs-49004/v1 ◽

2020 ◽

Author(s):

Li Xue ◽

Ming Li ◽

Li Tao ◽

Xueyi Li ◽

Wei Wang ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Disease Activity ◽

Roc Curve ◽

Degradation Product ◽

Plasma Fibrinogen ◽

Active Group ◽

Fibrin Degradation Product ◽

Logistical Regression ◽

Inactive Group ◽

D Dimer

Abstract Objective: This study aimed to assess the role of coagulation-related indicators such as plasma ﬁbrinogen (FIB), D-dimer, and fibrin degradation product (FDP) in rheumatoid arthritis (RA) and their association with disease activity.Methods: Data from 105 RA patients and 102 age- and gender- matched healthy controls were collected in the retrospective study. Disease activity score in 28 joints based on C-reactive protein (DAS28-CRP) was used to divide the RA patients into inactive group (DAS28-CRP ≤ 2.7) and active group (DAS28-CRP > 2.7). The association between plasma FIB, D-dimer, and FDP and DAS28-CRP was evaluated by spearman correlation. Receiver operating characteristic (ROC) curve was applied to determine the area under curve (AUC) value. The prognostic value of plasma FIB, D-dimer, and FDP in the RA disease activity was tested by logistical regression analysis.Results: RA patients showed higher FAR levels of plasma FIB, D-dimer, and FDP than the controls (P < 0.01). Plasma FIB, D-dimer, and FDP were also increased in active group of RA patients than those in inactive group (P < 0.001). Spearman analysis showed that plasma FIB, D-dimer, and FDP were positively related with DAS28-CRP (P < 0.001) in RA patients. ROC curve analyses revealed that the AUC of D-dimer was higher than ESR and RF, and that of FDP was higher than RF in RA patients. In addition, the optimal cut-oﬀ value of plasma FIB, D-dimer, and FDP for RA diagnosis was 286 mg/dL, 470 μg/L, and 1.45 mg/L, respectively. Logistical regression analyses showed that D-dimer (odds ratio = 2.862, 95% conﬁdence interval: 1.851-4.426, P < 0.001) was a predictor for RA disease activity.Conclusions: FIB, D-dimer, and FDP were increased in RA patients and positively correlated with the disease activity of RA. D-dimer may act as a novel inﬂammatory parameter for predicting disease activity in RA patients.

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The Involvement of Neutrophil Extracellular Traps in Disease Activity Associated With IgA Vasculitis

Frontiers in Immunology ◽

10.3389/fimmu.2021.668974 ◽

2021 ◽

Vol 12 ◽

Author(s):

Xiu-Qi Chen ◽

Li Tu ◽

Jia-Sen Zou ◽

Shi-Qun Zhu ◽

Yan-Jun Zhao ◽

...

Keyword(s):

Disease Activity ◽

Drug Withdrawal ◽

Neutrophil Extracellular Traps ◽

Dnase I ◽

Healthy Children ◽

Healthy Controls ◽

Iga Vasculitis ◽

Extracellular Traps ◽

Circulating Levels

ObjectivesThis aim of this study was to determine whether neutrophil extracellular traps (NETs) are involved in the pathogenesis of IgA vasculitis (IgAV) and investigate whether the circulating NETs levels are associated with disease activity in children.MethodsWe performed a case-control study and collected blood samples from 193 children with different stages of IgAV (61 were at the onset stage, 64 at the remission stage, 43 at the active stage, and 25 were undergoing drug withdrawal). A total of 192 healthy children were recruited as controls. Circulating cell free DNA (cf-DNA) was obtained from the plasma and quantified by using the Quant-iT PicoGreen DNA quantification kit. NETs-associated myeloperoxidase-DNA (MPO-DNA), citrullinated-histone H3 (cit-H3), neutrophil elastase (NE), and the deoxyribonuclease I (DNase I) concentrations were measured using enzyme-linked immunosorbent assays. The presence of NETs in the kidney and gastrointestinal tissues of onset and active IgAV patients was determined by multiple immunofluorescence staining in 15 IgAV nephritis patients and 9 IgAV patients without IgAV nephritis, respectively. NETs degradation potency of collected sera samples from IgAV patients were checked in vitro. Relationships between circulating levels of cf-DNA with MPO-DNA, NE, and DNase I and the patients were analyzed.ResultsCirculating levels of cf-DNA in onset and active IgAV patients were significantly higher than those in remission and drug withdrawal patients as well as healthy controls. The results were similar for MPO-DNA and NE. The levels of circulating cf-DNA correlated significantly with MPO-DNA, NE and DNase I. A significantly decreased degradation of NETs from the onset and active IgAV patients was observed, but was normal in healthy controls. Furthermore, presence of NETs was also confirmed in all renal and gastrointestinal tissues obtained from the onset and active IgAV patients but not control samples.ConclusionsOur data showed that NETs were released into the circulation of IgAV patients and are involved in the disease activity. The circulating levels of NETs maybe used to assess disease severity in children with IgAV.

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