Diabetogenic Potential of Dexamethasone and Effect of Annona Muricata Methanolic Bark Extract as Post-exposure Therapy in Albino Rats

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 131-LB
Author(s):  
TUNBOSUN A. OLADIPO ◽  
YEMISI D. ADEOYE ◽  
OLUWAMAYOWA O. OLADIPO ◽  
JOHNNY O. OLUKUNLE
Keyword(s):  
Exposure Therapy ◽  
Bark Extract ◽  
Albino Rats ◽  
Annona Muricata ◽  
Post Exposure

scholarly journals Diabetogenic potential of dexamethasone and effect Annona muricata methanolic bark extract as post-exposure therapy in albino rats

2020 ◽  
Vol 46 (2) ◽  
pp. 72-79
Author(s):  
T. A. Oladipo ◽  
O. E. Adeleye ◽  
J. Olorode ◽  
A. A. Adebiyi ◽  
O. O. Omobowale ◽  
...  
Keyword(s):  
Blood Sugar ◽  
Coagulation Necrosis ◽  
Bark Extract ◽  
Albino Rats ◽  
Therapeutic Effects ◽  
Annona Muricata ◽  
Post Exposure ◽  
Histopathological Lesions ◽  
Sugar Levels ◽  
Group B

Diabetes mellitus (DM) is a metabolic disorder leading to high level of morbidity and mortality in human population and it has been identified as the leading cause of death from non-infectious diseases. The use of dexamethasone has been on the increase due to wide array of therapeutic effects it has and the use has mostly been without prescription, since it is a non- prescription drug. Therefore, the potential of dexamethasone to induce DM was studied. Some of the drugs currently used in the treatment of diabetes have their own problematic effects and also expensive, Annona muricata methanolic bark extract(AMMBE) was used in the treatment of dexamethasone-induced diabetes in rats. The research was carried out on albino rats in Federal College of Animal Health and Production Technology, Ibadan and lasted for twenty-eight (28) days. The rats were allotted into four groups (A, B, C and D). Group B, C and D were induced with glucocorticoid (Dexamethasone) (2mgkg-1) daily for seven (7) days intraperitoneally, while group A which was the positive control were given distilled water throughout without induction with glucocorticoid. Group B (the negative control) was induced with glucocorticoid with noAMMBE administration. Group C, was induced and treated with AMMBE at 400 mgkg-1for 14 days. While Group D, was induced with glucocorticoid and were treated with the standard drug (glibenclamide) at 2.5mgkg-1body weight of the rats daily for 14 days. Organ samples of liver, kidney and pancreas were collected for histopathological lesions evaluation. The result showed that dexamethasone induced diabetes after seven (7) days of intraperitoneal administration of 2 mgkg-1 body weights with the glucometer readings in most of the albino rats up to and above 129 mgdL-1. The average blood sugar levels in induced groups (B, C and D) were 132.0±4.05, 129.0±1.41 and130.0±2.93, respectively which were not statistically significant (P>0.05). After administration of AMMBE, the average blood sugar level for group B (126.0±1.41) was significantly different (P<0.05) from C (91.0±1.72) and D (87.0±2.97). Clinical signs of alopecia, dehydration, writhing, paw-licking were observed. There were massive losses of pancreatic cell mass grossly afterinduction with dexamethasone. Histopathological lesions observed ranges from no visible lesion in the control and glibenclamide treated groups to accentuation of hepatocytes in the AMMBE treated rats, and marked vacuolar degeneration of hepatocytes in periportal areas to centrilobular area with Kidney degeneration and multifocal coagulation necrosis of tubular epithelium in group induced with dexamethasone but untreated (group B)Diabetogenic potential of dexamethasone and effect Annona muricata methanolic bark extract degeneration, multifoci coagulation and necrosis. The blood sugar levels post exposure to AMMBE and Glibenclamide showed reduction in the sugar levels. It could be concluded that dexamethasone has the potential of inducing diabetes when its use is prolonged and AMMBE has antidiabetic effect which could be fully explored.

scholarly journals Evaluation of spermatogenic activity of Annona muricata methanolic bark extract in Albino rats

2018 ◽  
Vol 49 (2) ◽  
pp. 83-90
Author(s):  
Tunbosun Oladipo ◽  
Adeniyi Adebiyi ◽  
Abiodun Adetomiwa ◽  
Oluwamayowa Oladipo ◽  
Olushola Adeleye ◽  
...  
Keyword(s):  
Bark Extract ◽  
Albino Rats ◽  
Annona Muricata

Post-exposure cortisol administration does not augment the success of exposure therapy: A randomized placebo-controlled study

2019 ◽  
Vol 99 ◽  
pp. 174-182 ◽  
Author(s):  
Friederike Raeder ◽  
Christian J. Merz ◽  
Martin Tegenthoff ◽  
Oliver T. Wolf ◽  
Jürgen Margraf ◽  
...  
Keyword(s):  
Exposure Therapy ◽  
Controlled Study ◽  
Post Exposure

Post-exposure therapy of inhalational anthrax in the common marmoset

2011 ◽  
Vol 38 (1) ◽  
pp. 60-64 ◽  
Author(s):  
Michelle Nelson ◽  
Anthony J. Stagg ◽  
Daniel J. Stevens ◽  
Mark A. Brown ◽  
Peter C. Pearce ◽  
...  
Keyword(s):  
Exposure Therapy ◽  
Common Marmoset ◽  
Post Exposure ◽  
The Common

scholarly journals Comparative evaluation of anti-diabetic action and pancreatic histopathology of rats treated with two alkaloidal plant extracts

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1841-1846
Author(s):  
Bonagiri Sreedevi ◽  
Vijaya Kuchana ◽  
Shobharani S
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Bark Extract ◽  
Albino Rats ◽  
Histopathological Study ◽  
Standard Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Soxhlet Apparatus ◽  
Ethanolic Extracts

This study aimed to understand Strychnosnuxvomica and Holarrhena pubescens Stem bark extract action towards M3 receptor in controlling blood glucose levels. Strychnos nux vomica  and Holarrhena pubescens are both alkaloidal drugs can help in controlling Hyperglycemic level. This will be useful in the formulation of a new herbal drug molecule for treating diabetes. Chloroform and ethanolic extracts of selected alkaloidal plants were extracted using the soxhlet apparatus and obtained quotes were tested for acute toxicity studies and carried out anti-diabetic action on Wister albino rats for 21 days. Results obtained from Blood glucose levels and histopathological study of test groups are compared with blood glucose levels of standard group, and highly significant action was identified by the chloroform extract of Strychnos nux vomica and Holarrhena pubescens group. Moderate anti-diabetic action was observed remaining two groups of ethanolic extracts. Strychnos nux vomica and Holarrhena pubescens ethanolic extract groups are acting on M3 receptors and controlling Hyperglycemic levels.

Pharmacological Evaluation of Antipyretic, Analgesic and Anti-inflammatory Activities of Ethanolic Extract of Cassia fistula

2021 ◽  
Author(s):  
D.K. Sharma ◽  
S.K. Sharma ◽  
M.K. Lonare ◽  
Rajdeep Kaur ◽  
V.K. Dumka
Keyword(s):  
Analgesic Activity ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Control Group ◽  
Albino Rats ◽  
Hot Plate ◽  
Antipyretic Activity ◽  
Tail Immersion ◽  
Anti Inflammatory

Background: The antipyretic, analgesic and anti-inflammatory activities of two concentrations (100 and 200 mg/kg) of ethanolic extract of leaf, bark, flower and fruit pulp of C. fistula were determined in male wistar albino rats. Methods: Antipyretic activity was assessed by E. coli endotoxin induced pyrexia. Analgesic activity was assessed by hot plate, tail immersion and acetic acid induced writhing test. Anti-inflammatory activity was evaluated by carrageenan-induced rat paw edema assay. Result: Significant (p less than 0.05) antipyretic activity was exhibited from 2h onwards by bark extract @ 200 mg/kg and from 3h onwards by bark extract @100 mg/kg and leaves extract @ 200mg/kg as compared to control group. Significant (p less than 0.05) analgesic activity was shown by extract of bark @ 200 mg/kg as it is evident by increase in reflex time in hot plate (90,120,180 min), tail immersion test (120,180 min) and inhibition of writhing (32.12%). Significant (p less than 0.05) anti-inflammatory activity was exhibited from 3h post administration by bark @ 200 and leaves @ 100 and 200 mg/kg.

scholarly journals EFFECT OF ETHANOL STEM BARK EXTRACT OF BERLINIA GRANDIFLORA HUTCH AND DALZ ON MARKER LIVER ENZYMES IN RATS TREATED WITH CCl4

2018 ◽  
Vol 10 (10) ◽  
pp. 69
Author(s):  
Martin Ntiamoah Donkor ◽  
Samuel Yawson Ayikanle ◽  
Samuel Adoesomdonkor
Keyword(s):  
Olive Oil ◽  
Liver Enzymes ◽  
Day Treatment ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Bark Extract ◽  
Albino Rats ◽  
Significant Activity ◽  
Stem Bark Extract ◽  
The Impact

Objective: The objective of this work was to validate the traditional claim of the use of the stem bark of Berlinia grandiflora in treating liver problems by investigating the effect of its ethanol stem bark extract on maker liver enzymes in vivo.Methods: Groups of Wistar albino rats (6 in each group) were daily treated with CCl4 (2 ml/kg b.w., diluted with olive oil 1:1 v/v, intravenously), followed by oral administration of ethanol stem bark extract of Berlinia grandiflora (ESBG) at doses 100, 300 and 900 mg/kg b.w. respectively, 3 h after the administration of the CCl4. Serum biochemical parameters were measured 24 h at the end of the 7-day treatment period and compared to a group intoxicated with CCl4 (2 ml/kg b.w., diluted with olive oil 1:1 v/v, intravenously) alone.Results: Treatment of rats with ESBG (at doses 100, 300 and 900 mg/kg b.w. orally) reduced the impact of CCl4-induced hepatotoxicity on liver maker enzymes Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP) and total bilirubin (TB), with the 900 mg/kg b.w. showing a significant reduction (p<0.05) compared to the group intoxicated with CCl4. The extract reversed the impact of the CCl4-induced hepatotoxicity by increasing the concentration of the serum Total protein (TP) and albumin (ALB), with the 900 mg/kg b.w. dose showing a significant increment (p<0.05) compared to the group intoxicated with the CCl4.Conclusion: The investigation of the effect of Berlinia grandiflora on CCl4 induced liver damage revealed that the ethanol extract of the stem bark of the plant was able to reverse the hepatotoxicity, with the 900 mg/kg b.w. dose showing a significant activity. 

Attenuation of Helicteres isora L. bark extracts on streptozotocin-induced alterations in glycogen and carbohydrate metabolism in albino rats

2009 ◽  
Vol 28 (11) ◽  
pp. 689-696 ◽  
Author(s):  
G. Kumar ◽  
G. Sharmila Banu ◽  
AG Murugesan
Keyword(s):  
Skeletal Muscle ◽  
Carbohydrate Metabolism ◽  
Glycogen Content ◽  
Bark Extract ◽  
Albino Rats ◽  
Glucose Levels ◽  
Helicteres Isora ◽  
Insulin Dependent ◽  
Hypoglycemic Drug ◽  
Normal Controls

The present study was undertaken to assess the effect of Helicteres isora L. on four important enzymes of carbohydrate metabolism (glucokinase [GK], hexokinase [HK] phosphofructokinase [PFK] and fructose-1, 6-bisphosphatase [FBP]) along with glycogen content of insulin-dependent (skeletal muscle and liver) and insulin-independent tissues (kidneys and brain) in streptozotocin (STZ; 60 mg/kg)-induced model of diabetes for 30 days. Administration of bark extracts (100, 200 mg/kg) for 30 days led to decrease in plasma glucose levels by approximately 9.60% and 22.04% and 19.18% and 33.93% on 15th and 30th day, respectively, of the experiment. Liver and two-kidney weight expressed as percentage of body weight significantly increased in diabetics (P < 0.05) versus normal controls. Renal glycogen content increased by 10 folds while hepatic and skeletal muscle glycogen content decreased by 75% and 68% in diabetic controls versus controls. H. isora did not affect glycogen content in any tissue. The decreased activities of PFK, GK, FBP and HK in diabetic controls were 40%, 50%, 50% and 60% and bark extract of H. isora partially corrected this alteration. The efficacy of the bark extract was comparable with Tolbutamide, a well-known hypoglycemic drug.

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