scholarly journals Comparative evaluation of anti-diabetic action and pancreatic histopathology of rats treated with two alkaloidal plant extracts

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1841-1846
Author(s):  
Bonagiri Sreedevi ◽  
Vijaya Kuchana ◽  
Shobharani S
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Bark Extract ◽  
Albino Rats ◽  
Histopathological Study ◽  
Standard Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Soxhlet Apparatus ◽  
Ethanolic Extracts

This study aimed to understand Strychnosnuxvomica and Holarrhena pubescens Stem bark extract action towards M3 receptor in controlling blood glucose levels. Strychnos nux vomica  and Holarrhena pubescens are both alkaloidal drugs can help in controlling Hyperglycemic level. This will be useful in the formulation of a new herbal drug molecule for treating diabetes. Chloroform and ethanolic extracts of selected alkaloidal plants were extracted using the soxhlet apparatus and obtained quotes were tested for acute toxicity studies and carried out anti-diabetic action on Wister albino rats for 21 days. Results obtained from Blood glucose levels and histopathological study of test groups are compared with blood glucose levels of standard group, and highly significant action was identified by the chloroform extract of Strychnos nux vomica and Holarrhena pubescens group. Moderate anti-diabetic action was observed remaining two groups of ethanolic extracts. Strychnos nux vomica and Holarrhena pubescens ethanolic extract groups are acting on M3 receptors and controlling Hyperglycemic levels.

scholarly journals PRELIMINARY PHYTOCHEMISTRY AND ANTIDIABETIC ACTIVITY OF PORTULACA GRANDIFLORA HOOK PLANT EXTRACT ON STREPTOZOTOCIN-INDUCED DIABETES IN RATS

2019 ◽  
pp. 87-90
Author(s):  
DEVI M ◽  
KOMAL S ◽  
LOGESHWARI B
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Portulaca Grandiflora ◽  
Streptozotocin Induced Diabetes

Objective: The present study was aimed to evaluate the antidiabetic activity of ethanolic extract of the whole aerial plant of Portulaca grandiflora Hook on streptozotocin (STZ)-induced diabetic rats. Methods: Experimental diabetes was induced by a single dose of intraperitoneal injection of STZ (150 mg/kg). Adult male Wister albino rats were divided into five groups; normal control, diabetic control, diabetic glibenclamide (5 mg/kg), diabetic P. grandiflora H. extract (200 mg/kg), and diabetic P. grandiflora H. extract (400 mg/kg) for 21 days and analyzed for body weight (BW) and blood glucose. Results: The STZ-treated diabetic control rats showed a significant increase in blood glucose with a concomitant decrease in BW. Oral administration of P. grandiflora H. extract (200 and 400 mg/kg) and glibenclamide (5 mg/kg) for 21 days showed a significant reduction in blood glucose levels and elevation in the bodyweight studies as compared to control and glibenclamide-treated rats. Conclusion: The results of the present study showed that a potent antidiabetic activity was present in the aerial part of plant P. grandiflora H. extract.

scholarly journals Effect of turmeric on alloxan induced diabetes mellitus in albino rats

2019 ◽  
Vol 8 (2) ◽  
pp. 264
Author(s):  
Mugdha Rajeeva Padhye ◽  
Sangita Devrao Jogdand
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Adverse Effects ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Organ Systems ◽  
Mean Blood Glucose

Background: Diabetes mellitus is a chronic metabolic disorder of glucose metabolism characterised by hyperglycaemia. Long standing diabetes mellitus leads to various complications affecting multiple organ systems. Management of diabetes mellitus includes lifestyle modification and pharmacotherapy. Pharmacotherapy of diabetes mellitus includes a wide variety of drugs that help in achieving adequate glycaemic control. Anti-diabetic medications are however associated with several adverse effects. Phytochemicals are being used extensively for the treatment of various diseases. Use of phytochemicals would minimize adverse effects due to various anti-diabetic drugs and improve patient compliance. In the present study, authors studied the effect of turmeric on alloxan induced diabetes mellitus in albino rats.Methods: Albino Wistar rats of either sex weighing 180 - 250grams were utilized for the present study. Diabetes mellitus was induced by intraperitoneal administration of alloxan. Ethanolic extract of turmeric was administered to diabetic rats daily orally for duration of 28 days. Blood glucose levels were monitored using glucometer before and after intervention with turmeric.Results: Statistically significant reduction in mean blood glucose levels (p value <0.05) was seen after intervention with turmeric in diabetic rats. There was a significant reduction in mean blood glucose levels.Conclusions: Ethanolic extract of turmeric showed antihyperglycemic effect in diabetic rats.

scholarly journals Detection of insulin-like protein and some active compounds in Bauhinia variegata Linn. leaf ethanolic extracts and the effect in reducing blood glucose levels in mice

2014 ◽  
Vol 8 (2) ◽  
pp. 76-83
Author(s):  
Khaleel Ibrahim Rashid
Keyword(s):  
Blood Glucose ◽  
High Performance ◽  
Ethanolic Extract ◽  
Diabetes In Animals ◽  
Active Compounds ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Ethanolic Extracts ◽  
High Reduction ◽  
Bauhinia Variegata

The study aims to detect the insulin-like protein and determine the active compounds in Bauhinia variegata L. leaves ethanolic extracts that help in reducing the blood glucose levels for white albino mice. The chemical detection of leaves ethanolic extract revealed the presence of tannins, terpenes, steroids, and flavonoids as active compounds. High performance liquid chromatography (HPLC) analysis method, using C18 column resulted in presence of insulin-like protein in the leaves extracts. Results showed significant reduction of blood glucose levels after 3 days of the treatment, and high reduction obtained after 6, 9, 12 days as compared with the human swine insulin used as a control. It was concluded that the crude ethanolic extracts and the partial purified insulin of B. variegata was used to examine the existence of insulin-like protein in this plant. In this study, an attempt has been made to report plant insulin that may be useful to the health professionals and scholars working in the field of pharmacology and therapeutics to develop alternative medicine to cure diabetes in animals and man.

scholarly journals Antidiabetic Effect of Pleurotus ostreatus (Jacq.ex Fr) kumm. Mushroom on Alloxan-induced Diabetic Rats.

2013 ◽  
Vol 1 (02) ◽  
pp. 31-36
Author(s):  
Imoh Johnny ◽  
Joseph Okon
Keyword(s):  
Blood Glucose ◽  
Pleurotus Ostreatus ◽  
Lethal Dose ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Reference Drug ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Treatment Dose

Antidiabetic effects of ethanolic extract of Pleurotus ostreatus (mushroom) on alloxan-induced diabetic rats was studied. The median lethal dose (LD50) of the extract was determined to be 3,472.14 mgkg-1 and a single dose of 380.0, 760.0 and 1140.0 body weight of the extract were intraperitoneally administered as the treatment dose and the blood glucose levels (BGL) examined for 7 hours and 15 hours (prolonged) at 2 and 4 hours intervals respectively. The extract exhibited significant (p 0.05 and p 0.01) reduction in the blood glucose levels of the albino rats. The extract compared favourably with the standard reference drug (metformin) which all gave their maximum BGL reduction at 5 hours duration. The confirmation of antidiabetic potentials of the Pleurotus ostreatus tuber has been justified in this study as claimed by traditional medicine practitionersin Akwa Ibom State.

Gynura divaricata Effect on Blood Glucose Levels in Alloxan-Induced Diabetes Mice

2019 ◽  
Vol 13 (01) ◽  
pp. 27-33
Author(s):  
Evi Sovia ◽  
Dian Anggraeny ◽  
Ris Kristiana ◽  
Firhan Hamdi Maulida ◽  
Mutia Susparini
Keyword(s):  
Blood Glucose ◽  
Histological Examination ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Diabetes Mice ◽  
Before And After

Gynura divaricata (GD) also known as Dewa leaf is one of the Indonesian medicinal plants which are also native to India, Thailand and China. GD is widely used as antidiabetic, antihypertensive and other diseases including several tumors. However, research on the effects of GD as antidiabetic is still not widely practiced, especially in Indonesia. The aim of this research is to investigate the effect of GD ethanolic extract on blood glucose levels of alloxan-induced diabetes mice. This research is an experimental research with pre- and post-test design using 24 male DDY strain mice. Animals experiment is divided into four groups, that are one normal group and three alloxan-induced diabetes mice group that give 1% CMC (control), 400[Formula: see text]mg/kg GD ethanolic extract and 0.65[Formula: see text]mg/kg glibenclamide, respectively. Treatment was given for 14 days. Blood glucose levels were measured before and after inducing alloxan, and after 14 days treatment. At the end of study, all of animals experiment were sacrificed for histological examination. Phytochemical analysis revealed that polyphenols, flavonoids, steroids, triterpenoids, terpenoids and and quinones were present in ethanol extract of GD. The results of this study showed significant ([Formula: see text]) decrease of blood glucose levels after GD ethanolic extract and glibenclamide treatment. The results of histological examination showed improvement in pancreas damage and significant increase in the number of beta cells in GD and glibenclamide groups. The results indicate that dewa leaf ethanolic extract has antidiabetic activity and the effectiveness is the same as glibenclamide. GD also could repair damage of pancreas caused by alloxan induction.

scholarly journals Taxithelium napalense acts against free radicals and diabetes mellitus

2017 ◽  
Vol 12 (2) ◽  
pp. 16 ◽  
Author(s):  
Vinay Bharadwaj Tatipamula ◽  
Kishore Naidu Killari ◽  
Vedula Girija Sastry ◽  
Alekhya Ketha
Keyword(s):  
Free Radicals ◽  
Blood Glucose ◽  
Video Clip ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Radical Scavenging Properties ◽  
Histopathological Studies

<p class="Abstract">In this work, <em>Taxithelium nepalense</em> ethanolic extract and its fractions were evaluated for the antidiabetic activity in rat based on phytochemical and free radical scavenging properties. The ethanolic extract, fraction IV and V significantly attenuated the blood glucose levels at 600, 200 and 200 mg/kg with 50.0, 33.5 and 42.0% inhibition respectively. The histopathological studies were manifesting the recuperation of damaged cells in liver and pancreas tissues. The outcomes of the present work affirm that the <em>T. nepalense</em> has a potency to plummet the overproduction of free radicals and blood glucose levels in the diabetic-induced rat.</p><p><strong>Video Clip of Methodology</strong>:</p><p>1 min 50 sec   <a href="https://youtube.com/v/GUZp4QY9kCs">Full Screen</a>   <a href="https://youtube.com/watch?v=GUZp4QY9kCs">Alternate</a></p>

scholarly journals WOUND HEALING POTENTIAL OF METHANOLIC EXTRACT OF FLOWERS OF BUTEA MONOSPERMA LINN. IN DIABETIC ANIMALS

2018 ◽  
Vol 8 (5-s) ◽  
pp. 306-310
Author(s):  
Shweta Panwar ◽  
Neetesh Kumar Jain ◽  
MK Gupta
Keyword(s):  
Wound Healing ◽  
Blood Glucose ◽  
Methanolic Extract ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Butea Monosperma ◽  
Glucose Levels ◽  
Wound Model ◽  
Soxhlet Apparatus ◽  
Alloxan Monohydrate

The main of our study is to evaluate the Wound Healing Potential of Methanolic Extract of Flowers of Butea monosperma Linn. in Diabetic Animals. Methanolic extract was prepared by continuous hot extraction method by soxhlet apparatus. Preliminary phytochemical screening showed the presence of flavonoids, phenolic compounds and some glycosides. Diabetes was induced by single injection of Alloxan monohydrate in Wistar albino rats and their blood glucose levels were measured. Excision wound model was used for creation of wound in diabetic animals and methanolic extract was administered in diabetic animals to observe its effect. Methanolic extract significantly lowered the blood glucose level and highly significantly showed wound contraction in diabetic animals. The preliminary phytochemical analysis of the Butea monosperma flower extract showed the presence of tannins, flavonoids and triterpenoids. As per previous literature survey, flavonoids are responsible for anti-diabetic and wound healing activity. So this wound healing effect of Butea monosperma may be due to presence of flavonoids in plant. Hence present research supports traditional claims of the plant in wound healing. Keywords: Butea monosperma, Diabetic animals, Alloxan monohydrate, Methanolic Extract, Wound Area

In vitro and in vivo anti-diabetic activity of Citrullus colocynthis pulpy flesh with seeds hydro-ethanolic extract

2020 ◽  
Vol 17 (2) ◽  
Author(s):  
Aymen Owais Ghauri ◽  
Saeed Ahmad ◽  
Tayyeba Rehman
Keyword(s):  
Blood Glucose ◽  
Normal Control ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Antioxidant Potential ◽  
Blood Glucose Monitoring ◽  
Glucose Levels ◽  
Blood Glucose Levels

AbstractBackgroundDiabetes is the one of the leading cause of morbidity and mortality. Traditionally phytotherapy is widely being used for diabetes treatment and highly valued. Citrus colocynthis has known anti-diabetic potential. However, anti-diabetic potential of hydro-ethanolic extract of C. colocynthis pulpy flesh with seeds is not reported yet.MethodsThe extract of C. colocynthis pulpy flesh with seeds was done by maceration method using 70% ethanol. To evaluate anti-diabetic and antioxidant potential of the seeded fruit in vitro, α-glucosidase and DPPH inhibition assays was done, respectively. In vivo study used streptozotocin (STZ) induced diabetes model of rats. Rats were randomized in five groups i. e. normal control, negative control, standard control, C. colocynthis 150 and 300 mg/kg. STZ was administered to all groups except normal control. After wards, plant extract and glibenclamide is continued for 14 days. Blood samples were collected from rat tail vein daily and from Cardiac puncture at the end of study. The blood glucose levels were monitored daily by using one-touch blood glucose monitoring system. The blood glucose level was monitored on 0, 1st, 5th, 8th, 11th, and 14th day of induction.ResultsHydro-ethanolic extract of C. colocynthis pulpy flesh with seeds was able to decolorize DPPH and therefore possess antioxidant potential, continuous administration for 14 days showed a marked decrease in serum glucose levels (p 0.01) it is found to be somewhat less effective as glibenclamide (standard control) (p 0.001). A time-dependent decrease in blood glucose levels was observed (351.3 ± 4 to 258 m/kg).ConclusionHydro-ethanolic extract of C. colocynthis pulpy flesh with seeds lowered the serum triglyceride and cholesterol levels in diabetic rats significantly as compared to negative control. The hypoglycemic effect of hydro-ethanolic extract of C. colocynthis pulpy flesh with seeds is may be due to α-glucosidase inhibition potential.

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