Adherence of Type 1-Fimbriated Escherichia coli to Uroepithelial Cells: More in diabetic women than in control subjects

Uropathogenic Escherichia coli (UPEC) is the most common pathogenic bacteria associated with urinary tract infection (UTI). UPEC can cause UTI by adhering to and invading uroepithelial cells. Fimbriae is the most important virulence factor of UPEC, and a potentially promising target in developing novel antibacterial treatments. In this study, the antibacterial properties and effects of the compound dictamnine, extracted from the traditional Chinese medicine Cortex Dictamni, on the bacterial morphology, cell adhesion, and invasion of UPEC were studied. Dictamnine exhibited no obvious antibacterial activity against UPEC, but significantly impeded the ability of UPEC to adhere to and invade uroepithelial cells. RT-qPCR analysis showed that treatment downregulated the expression of type 1 fimbriae, P fimbriae, and curli fimbriae adhesion genes, and also downregulated adhesion-related receptor genes of uroepithelial cells. Transmission electron microscopy showed that dictamnine destroyed the structure of the fimbriae and the surface of the bacteria became smooth. These results suggest that dictamnine may help to prevent UTI by simultaneously targeting UPEC fimbriae and urothelial adhesin receptors, and may have a potential use as a new anti-UPEC drug.

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Distribution of fimH Gene in Local Isolates of Adhesive Uropathogenic Escherichia coli

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v9i3.9 ◽

2019 ◽

Vol 9 (o3) ◽

Author(s):

Omar Abdulkareem Ali ◽

Ruqayah Qubtan Taha

Keyword(s):

Escherichia Coli ◽

Virulence Factors ◽

Biofilm Formation ◽

Uropathogenic Escherichia Coli ◽

Adhesion Assay ◽

E Coli ◽

Type 1 Fimbriae ◽

Formation Type ◽

Uroepithelial Cells

Adhesion is an influential step for bacterial vigor in clinical micro-environments, type 1 fimbriae are essential virulence factors help uropathogenic E. coli in invasion and colonization of uroepithelial cells, the first step of UTIs and biofilm formation. Type 1 fimbriae of E. coli contain FimH protein at the tip encoding via fimH gene cluster, this study was conducted for determination the fimH gene distribution in uro-pathogenic E. coli isolated from UTIs patients. The results of adhesion assay show that (83.6%) of uropathogenic E. coli were high adherent isolates. While the results of E. coli fimH gene amplification prove that, of all E. coli isolates, the fimH gene was found in (87.1%), while among high adherent isolates it was found in (92.6%), and that Shows the function of type 1 fimbriae in the colonization and infection of urinary tracts in addition to other adhesions virulence agents of uropathogenic E. coli.

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Faculty Opinions recommendation of Adaptive mutations in the signal peptide of the type 1 fimbrial adhesin of uropathogenic Escherichia coli.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1119456.575652 ◽

2008 ◽

Author(s):

Esther Bullitt

Keyword(s):

Escherichia Coli ◽

Signal Peptide ◽

Uropathogenic Escherichia Coli ◽

Adaptive Mutations ◽

Fimbrial Adhesin

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Crystal Structure of the B Subunit of Escherichia coli Heat-labile Enterotoxin Carrying Peptides with Anti-herpes Simplex Virus Type 1 Activity

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)87394-5 ◽

1999 ◽

Vol 274 (13) ◽

pp. 8764-8769

Author(s):

Dubravka Matković-Calogović ◽

Arianna Loregian ◽

Maria Rosa D'Acunto ◽

Roberto Battistutta ◽

Alessandro Tossi ◽

...

Keyword(s):

Crystal Structure ◽

Escherichia Coli ◽

Herpes Simplex Virus ◽

Herpes Simplex ◽

Virus Type ◽

Herpes Simplex Virus Type ◽

B Subunit ◽

Heat Labile ◽

Simplex Virus

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Parent Report of Mealtime Behavior and Parenting Stress in Young Children With Type 1 Diabetes and in Healthy Control Subjects

Diabetes Care ◽

10.2337/diacare.25.2.313 ◽

2002 ◽

Vol 25 (2) ◽

pp. 313-318 ◽

Cited By ~ 110

Author(s):

S. W. Powers ◽

K. C. Byars ◽

M. J. Mitchell ◽

S. R. Patton ◽

D. A. Standiford ◽

...

Keyword(s):

Type 1 Diabetes ◽

Young Children ◽

Parenting Stress ◽

Parent Report ◽

Control Subjects ◽

Mealtime Behavior ◽

Healthy Control

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Comparison of colony and stool blots for detection of enteropathogens by DNA probes

Canadian Journal of Microbiology ◽

10.1139/m91-066 ◽

1991 ◽

Vol 37 (5) ◽

pp. 407-410

Author(s):

Mônica A. M. Vieira ◽

Beatriz E. C. Guth ◽

Tânia A. T. Gomes

Keyword(s):

Escherichia Coli ◽

Key Words ◽

Sensitivity And Specificity ◽

Dna Probes ◽

Type I ◽

Enteropathogenic Escherichia Coli ◽

Key Words Dna ◽

E Coli ◽

Heat Stable

DNA probes that identify genes coding for heat-labile type I (LT-I) and heat-stable type 1 (ST-I) enterotoxins, enteropathogenic Escherichia coli adherence factor (EAF), and Shigella-like, invasiveness (INV) are used to evaluate the sensitivity and specificity of stool blots in comparison with the sensitivity and specificity of colony blots in detecting enteropathoghens. The sensitivities of the probes in stool blots are 91.7% for the LT-I probe, 76.9% for the ST-I probes, 78.9% for the EAF probe, and 45.5% for the INV probe. The specificity of all probes is higher than 95%. In general, the stool blot method identifies as many if not more LT-I-, ST-I-, and EAF-producing E. coli infections than the colony blots. Key words: DNA probes, stool blots, enteropathogens, diagnosis.

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