Latex sensitization in patients with myelomeningocele: contribution of microarray technique

AbstractChanges in the level of cellular proteins in cells inoculated with equine influenza virus H7N7 and H3N8 were studied with microarray technique. H3N8 induced pro-apoptotic proteins while H7N7 induced both pro- as well as anti-apoptotic factors. The higher level of some cytoskeleton components and proteins involved in the protein quality control was recorded. Relatively high number of proteins involved in the regulation of transcription was down-regulated. The pattern of changes observed for H7N7 and H3N8 may reflect differences in the biological properties of both serotypes.

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An electronic DNA microarray technique for detection and differentiation of viable Campylobacter species

The Analyst ◽

10.1039/b603315f ◽

2006 ◽

Vol 131 (8) ◽

pp. 907 ◽

Cited By ~ 4

Author(s):

Hai Zhang ◽

Zhilong Gong ◽

Odell Pui ◽

Yanming Liu ◽

Xing-Fang Li

Keyword(s):

Dna Microarray ◽

Campylobacter Species ◽

Microarray Technique

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Whole-Genome Analysis of Genes Regulated by the Bacillus subtilis Competence Transcription Factor ComK

Journal of Bacteriology ◽

10.1128/jb.184.9.2344-2351.2002 ◽

2002 ◽

Vol 184 (9) ◽

pp. 2344-2351 ◽

Cited By ~ 110

Author(s):

Mitsuo Ogura ◽

Hirotake Yamaguchi ◽

Kazuo Kobayashi ◽

Naotake Ogasawara ◽

Yasutaro Fujita ◽

...

Keyword(s):

Transcription Factor ◽

Bacillus Subtilis ◽

Genetic Transformation ◽

Dna Microarray ◽

Genome Analysis ◽

Transformation Efficiency ◽

The Other ◽

Whole Genome ◽

Whole Genome Analysis ◽

Microarray Technique

ABSTRACT The Bacillus subtilis competence transcription factor ComK is required for establishment of competence for genetic transformation. In an attempt to study the ComK factor further, we explored the genes regulated by ComK using the DNA microarray technique. In addition to the genes known to be dependent on ComK for expression, we found many genes or operons whose ComK dependence was not known previously. Among these genes, we confirmed the ComK dependence of 16 genes by using lacZ fusions, and three genes were partially dependent on ComK. Transformation efficiency was significantly reduced in an smf disruption mutant, although disruption of the other ComK-dependent genes did not result in significant decreases in transformation efficiency. Nucleotide sequences similar to that of the ComK box were found for most of the newly discovered genes regulated by ComK.

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Differential expression of genes in human normal and breast cancer cells determined by microarray technique

Nature Genetics ◽

10.1038/14353 ◽

1999 ◽

Vol 23 (S3) ◽

pp. 59-59

Author(s):

Suxing Liu ◽

Qun Wu ◽

Paul Kirschmeier ◽

Terri McClanahan ◽

Johnathan Greene ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Differential Expression ◽

Breast Cancer Cells ◽

Expression Of Genes ◽

Microarray Technique ◽

Differential Expression Of Genes

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Gene Expression Profile of Colon Mucosa after Cytotoxic Insult in wt and Apc-Mutated Pirc Rats: Possible Relation to Resistance to Apoptosis during Carcinogenesis

BioMed Research International ◽

10.1155/2016/1310342 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Angelo Pietro Femia ◽

Cristina Luceri ◽

Maura Lodovici ◽

Stefania Crucitta ◽

Giovanna Caderni

Keyword(s):

Gene Expression ◽

Gene Expression Profile ◽

Expression Profile ◽

Antioxidant Status ◽

Genotoxic Stress ◽

Rt Pcr ◽

Induced Genes ◽

Stress Related Genes ◽

Microarray Technique ◽

Unsupervised Cluster Analysis

Apc-mutated Pirc rats, spontaneously developing intestinal tumours, are resistant to 1,2-dimethylhydrazine- (DMH-) induced colon apoptosis. To understand this phenomenon, we analyzed the expression of genotoxic stress-related genes Mgmt, Gsta1, and Gstp1 in the colon of wt and Pirc rats in basal conditions and 24 h after DMH; plasmatic oxidant/antioxidant status was also evaluated. After DMH, Mgmt expression was increased in both genotypes but significantly only in wt rats; Gsta1 expression was significantly increased in both genotypes. Gstp1 expression did not vary after DMH but was lower in Pirc rats. Moreover, for each genotype, we studied by microarray technique whole gene expression profile after DMH. By unsupervised cluster analysis, 28 genes were differentially modulated between the two genotypes. Among them were interferon-induced genes Irf7, Oas1a, Oasl2, and Isg15 and the transcription factor Taf6l, overexpressed in DMH-treated wt rats and unchanged in Pirc rats. RT-PCR confirmed their overexpression in DMH-treated wt rats and showed a slighter variation in DMH-treated Pirc rats. Taken together, despite a blunted induction of Irf7, Oas1a, and Mgmt, defective apoptosis in Pirc rats 24 h after DMH is not mirrored by major differences in gene expression compared with wt rats.

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