Iron Determination in Whole Blood Samples of Dystrophic Mouse Strains Using X-Ray Fluorescence Spectrometry

A standard-free method developed by ourselves has been applied to bio-medical samples of less than 1 mg or less than 1 μl, to which an ordinary internal standard method can not be accurately applied. As the result, correct values of potassium concentration in NIST-Bovine liver samples of nearly 0.5 mg were obtained. Furthermore, the standard-free method has been applied to small quantity of serum and whole-blood samples, and satisfactory results were obtained. For samples which easily peel off from a backing film, such as serum and whole blood, a carbon tape with an adhesive agent was also used as a backing film, and it is found that this method is also applicable. Moreover, the standard-free method was confirmed to be effective for a case where a specially designed x-ray absorber is used.

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Steadiness/stability of antiepileptic drugs in serum, plasma and whole-blood samples under various storage methods

Neuropediatrics ◽

10.1055/s-0030-1265593 ◽

2010 ◽

Vol 41 (02) ◽

Author(s):

N Shazi ◽

A Böss ◽

HJ Merkel ◽

F Scharbert ◽

D Hannak ◽

...

Keyword(s):

Antiepileptic Drugs ◽

Whole Blood ◽

Blood Samples ◽

Storage Methods ◽

Whole Blood Samples

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A New Method to Assess Platelet Activation and Leukocyte-platelet Aggregates in Whole Blood Samples by Flow Cytometry for Clinical Studies

10.1055/s-0039-1680171 ◽

2019 ◽

Author(s):

O. Hidiatov ◽

R. Jouni ◽

I. Marini ◽

A. Straub ◽

T. Bakchoul

Keyword(s):

Flow Cytometry ◽

Platelet Activation ◽

Whole Blood ◽

Clinical Studies ◽

New Method ◽

Blood Samples ◽

Platelet Aggregates ◽

Whole Blood Samples

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Collection of Human Liver Biopsy and Whole Blood Samples From T1DM, TP or PP Patients for Potential Use for Insulin Producing Cells in Future Clinical Studies

Case Medical Research ◽

10.31525/ct1-nct04038931 ◽

2019 ◽

Author(s):

Keyword(s):

Liver Biopsy ◽

Whole Blood ◽

Clinical Studies ◽

Human Liver ◽

Blood Samples ◽

Insulin Producing Cells ◽

Whole Blood Samples ◽

Potential Use

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Simultaneous detection of Epstein - Barr virus and Cytomegalovirus DNA in Mini-pooling of Whole blood Samples from Iraqi blood donors by Multiplex Real-Time PCR Assay

10.36295/asro.2020.231441 ◽

2020 ◽

Vol 23 (14) ◽

Author(s):

Ghinwa S. Majid ◽

Ahmed S. Abdulamir ◽

Abbas M. Ahmed ◽

Iman M. Aufi ◽

Orooba I. Abdullah

Keyword(s):

Real Time Pcr ◽

Whole Blood ◽

Epstein Barr Virus ◽

Blood Donors ◽

Simultaneous Detection ◽

Pcr Assay ◽

Blood Samples ◽

Barr Virus ◽

Whole Blood Samples ◽

Epstein Barr

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Determination of 19 Psychoactive Substances in Premortem and Postmortem Whole Blood Samples Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Separations ◽

10.3390/separations8060078 ◽

2021 ◽

Vol 8 (6) ◽

pp. 78

Author(s):

Sevasti Karampela ◽

Jessica Smith ◽

Irene Panderi

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Formic Acid ◽

Whole Blood ◽

High Performance ◽

Tandem Mass ◽

Psychoactive Substances ◽

Blood Samples ◽

Whole Blood Samples

An ever-increasing need exists within the forensic laboratories to develop analytical processes for the qualitative and quantitative determination of a broad spectrum of new psychoactive substances. Phenylethylamine derivatives are among the major classes of psychoactive substances available on the global market and include both amphetamine analogues and synthetic cathinones. In this work, an ultra-high-performance liquid chromatography-positive ion electrospray ionization tandem mass spectrometric method (UHPLC-ESI-MS/MS) has been developed and fully validated for the determination of 19 psychoactive substances, including nine amphetamine-type stimulants and 10 synthetic cathinone derivatives, in premortem and postmortem whole blood. The assay was based on the use of 1 mL premortem or postmortem whole blood, following solid phase extraction prior to the analysis. The separation was achieved on a Poroshell 120 EC-C18 analytical column with a gradient mobile phase of 0.1% formic acid in acetonitrile and 0.1% formic acid in water in 9 min. The dynamic multiple reaction monitoring used in this work allowed for limit of detection (LOD) and lower limit of quantitation (LOQ) values of 0.5 and 2 ng mL−1, respectively, for all analytes both in premortem and postmortem whole blood samples. A quadratic calibration model was used for the 12 quantitative analytes over the concentration range of 20–2000 ng mL−1, and the method was shown to be precise and accurate both in premortem and postmortem whole blood. The method was applied to the analysis of real cases and proved to be a valuable tool in forensic and clinical toxicology.

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