scholarly journals Heterologous demineralised bone matrix is efficient for the repair of critical-sized rat calvarial defects

2020 ◽  
Vol 5 (3) ◽  
pp. 24-34
Author(s):  
A. V. Veremeev ◽  
R. N. Bolgarin ◽  
V. G. Nesterenko ◽  
A. A. Andreev-Andrievskiy
Keyword(s):  
Bone Repair ◽  
Bone Matrix ◽  
Microcomputed Tomography ◽  
Demineralised Bone Matrix ◽  
Calvarial Defect ◽  
Sprague Dawley ◽  
Mineral Density ◽  
Calvarial Defects ◽  
Demineralised Bone ◽  
Bone Autograft

Aim. To evaluate the efficacy of heterologous demineralised bone matrix (DBM) for the replacement of bone defects using a critical-sized rat calvarial defect model. Materials and Methods. For the experiments, we used 48 Sprague-Dawley rats (4.5 to 6 months of age). Critical-sized (8 mm diameter) calvarial defect was filled by the bone autograft, heterologous DBM, or comparator product (Geistlich BioOss®) or remained unfilled (negative control). Upon 4 or 12 weeks, rats were euthanised with the subsequent investigation of the defect and adjacent tissues by means of hematoxylin and eosin staining (mineralized tissue area to the defect area ratio) and microcomputed tomography (volume, thickness, and mineral density of the repaired tissue). Results. In our experimental setting, bone autograft was the most efficient in bone repair. Heterologous DBM and comparator product were equally efficient in filling the defect and did not show any statistically significant differences regarding any of the parameters. Microcomputed tomography and routine histological examination demonstrated concordant results. Conclusion. Heterologous DBM is efficient for the repair of critical-sized rat calvarial defects.

scholarly journals Native Bovine Hydroxyapatite Powder, Demineralised Bone Matrix Powder, and Purified Bone Collagen Membranes Are Efficient in Repair of Critical-Sized Rat Calvarial Defects

Materials ◽  
2020 ◽  
Vol 13 (15) ◽  
pp. 3393 ◽  
Author(s):  
Alexey Veremeev ◽  
Roman Bolgarin ◽  
Vladimir Nesterenko ◽  
Alexander Andreev-Andrievskiy ◽  
Anton Kutikhin
Keyword(s):  
Bone Mineral ◽  
Bone Repair ◽  
Bone Matrix ◽  
Bone Collagen ◽  
Demineralised Bone Matrix ◽  
Bovine Bone ◽  
Mineral Density ◽  
Artificial Defect ◽  
Demineralised Bone ◽  
Mineral Powder

Here we evaluated the efficacy of bone repair using various native bovine biomaterials (refined hydroxyapatite (HA), demineralised bone matrix (DBM), and purified bone collagen (COLL)) as compared with commercially available bone mineral and bone autografts. We employed a conventional critical-sized (8 mm diameter) rat calvarial defect model (6-month-old male Sprague–Dawley rats, n = 72 in total). The artificial defect was repaired using HA, DBM, COLL, commercially available bone mineral powder, bone calvarial autograft, or remained unfilled (n = 12 animals per group). Rats were euthanised 4 or 12 weeks postimplantation (n = 6 per time point) with the subsequent examination to assess the extent, volume, area, and mineral density of the repaired tissue by means of microcomputed tomography and hematoxylin and eosin staining. Bovine HA and DBM powder exhibited excellent repair capability similar to the autografts and commercially available bone mineral powder while COLL showed higher bone repair rate. We suggest that HA and DBM powder obtained from bovine bone tissue can be equally applied for the repair of bone defects and demonstrate sufficient potential to be implemented into clinical studies.

scholarly journals Xenogeneic bone mineral is efficient for the repair of critical-sized rat calvarial defects

2021 ◽  
Vol 6 (1) ◽  
pp. 16-26
Author(s):  
A. V. Veremeev ◽  
R. N. Bolgarin ◽  
V. G. Nesterenko ◽  
A. A. Andreev-Andrievskiy
Keyword(s):  
Bone Graft ◽  
Bone Mineral ◽  
Bone Repair ◽  
3D Structure ◽  
Autologous Bone Graft ◽  
Bone Volume ◽  
Calvarial Defect ◽  
Sprague Dawley ◽  
Mineral Density ◽  
Autologous Bone

Aim. To evaluate the efficiency of bone repair on a critical-sized rat calvarial defect model using our original xenogeneic bone mineral, widely established Geistlich Bio-Oss®, and autologous bone graft.Materials and Methods. We created a critical-sized calvarial defect in Sprague-Dawley rats (n = 48) and then divided them into 4 groups (unfilled defect, autologous bone graft, Geistlich BioOss® and our original xenogeneic bone mineral, 12 rats per group). Rats were sacrificed upon 4 and 12 months (6 rats per time point) with the following excision of the implant and adjacent tissues. 3D structure, extent of mineralisation, and bone volume were measured by means of microcomputed tomography. Microanatomy of the explants and adjacent tissue was investigated by haematoxylin and eosin staining.Results. The highest and the lowest bone volume was expectedly detected when the defect was filled with the autologous bone graft or remained unfilled, respectively. Replacement of the defect by the original bone mineral entailed better regeneration as compared to Geistlich Bio-Oss. Bone mineral density, bone thickness and the extent of mineralisation did not differ significantly between the experimental groups and were close to the positive control values, indicating efficient bone repair.Conclusions. Original xenogeneic bone mineral promotes induction of bone regeneration as compared to Geistlich Bio-Oss®, a commercially available bone mineral widely used in the clinical practice.

scholarly journals Histological remodelling of demineralised bone matrix allograft in posterolateral fusion of the spine – an ex vivo study

BMC Surgery ◽  
2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Samy Bouaicha ◽  
Brigitte von Rechenberg ◽  
Georg Osterhoff ◽  
Guido A Wanner ◽  
Hans-Peter Simmen ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Bone Matrix ◽  
Posterolateral Fusion ◽  
Demineralised Bone Matrix ◽  
Demineralised Bone ◽  
Ex Vivo Study

Safety and efficacy of commercially available demineralised bone matrix preparations: A critical review of clinical studies

Injury ◽  
2007 ◽  
Vol 38 ◽  
pp. S13-S21 ◽  
Author(s):  
Georgios I. Drosos ◽  
Konstantinos I. Kazakos ◽  
Pavlos Kouzoumpasis ◽  
Dionisios-Alexandros Verettas
Keyword(s):  
Critical Review ◽  
Clinical Studies ◽  
Bone Matrix ◽  
Demineralised Bone Matrix ◽  
Safety And Efficacy ◽  
Demineralised Bone

scholarly journals Effect of platelet-rich plasma combined with demineralised bone matrix on bone healing in rabbit ulnar defects

2017 ◽  
Vol 58 (9) ◽  
pp. 551-556 ◽  
Author(s):  
V Galanis ◽  
A Fiska ◽  
S Kapetanakis ◽  
K Kazakos ◽  
T Demetriou
Keyword(s):  
Bone Healing ◽  
Platelet Rich Plasma ◽  
Bone Matrix ◽  
Demineralised Bone Matrix ◽  
Demineralised Bone

Demineralised bone matrix in veterinary orthopaedics: A review

2010 ◽  
Vol 23 (06) ◽  
pp. 393-399 ◽  
Author(s):  
P. Myint ◽  
J. F. Innes
Keyword(s):  
Cortical Bone ◽  
Experimental Animal ◽  
Basic Science ◽  
Animal Studies ◽  
Bone Matrix ◽  
Bone Allograft ◽  
Demineralised Bone Matrix ◽  
Demineralised Bone ◽  
Human Use

SummaryDemineralised bone matrix (DBM) is commonly used in human orthopaedics as an allograft prepared from cortical bone. As such, there is a background of literature on the basic science, experimental animal studies and clinical human use of DBM. Because canine DBM is now increasingly available and used in veterinary orthopaedics, this review aims to update the veterinary orthopaedic specialist with the properties and activities of this bone allograft product.

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