scholarly journals PENGARUH MEDIA LIMBAH PERTANIAN PADAT TERHADAP PERTUMBUHAN JAMUR Purpureocillium lilacinum (Syn. Paecilomyces lilacinus)

2021 ◽  
Vol 9 (3) ◽  
pp. 397
Author(s):  
Maruf Kurniawan ◽  
I Gede Swibawa ◽  
Solikhin Solikhin ◽  
Yuyun Fitriana
Keyword(s):  
Paecilomyces Lilacinus ◽  
Purpureocillium Lilacinum ◽  
Meloidogyne Spp

Pengaruh Media Limbah Pertanian Padat terhadap Pertumbuhan Jamur Purpureocillium lilacinum (Syn. Paecilomyces lilacinus). Jamur P. lilacinum (Syn. P. lilacinus) adalah jamur parasit telur nematoda puru akar (Meloidogyne spp.). Selain  sebagai musuh alami nematoda, jamur P. lilacinum (Syn. P. lilacinus) juga berperan sebagai dekomposer bahan organik. Limbah pertanian banyak yang digunakan untuk menumbuhkan jamur, sehingga berpotensi digunakan sebagai bahan pembawa pembuatan bionematisida berbahan aktif jamur. Penelitian ini bertujuan untuk mempelajari pertumbuhan jamur P. lilacinum (Syn. P. lilacinus) pada limbah pertanian padat kulit ubi ubikayu, bonggol pisang, beras dan campurannya. Rancangan Acak Kelompok (RAK) dengan 5 ulangan diterapkan dalam percobaan menggunakan jamur isolat dengan kode B4100. Data dianalisis ragam dan dilanjutkan dengan pemisahan nilai tengah menggunakan uji BNT pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa isolat P. lilacinum (Syn. P. lilacinus) tumbuh pada media limbah pertanian padat yang derajat keasamannya dimodifikasi dan nutrisinya ditambah. Pertumbuhan jamur yang paling baik dengan produksi spora yang paling tinggi terjadi pada media beras

scholarly journals PENGARUH DOSIS BIONEMATISIDA JAMUR Purpureocillium lilacinum (Syn. Paecilomyces lilacinus) ISOLAT B01TG BERBAHAN PEMBAWA LIMBAH PERTANIAN TERHADAP KEEFEKTIFANNYA DALAM MENGENDALIKAN Meloidogyne spp.

2021 ◽  
Vol 9 (2) ◽  
pp. 189
Author(s):  
Ambar Fiandani ◽  
I Gede Swibawa ◽  
Yuyun Fitriana ◽  
Purnomo Purnomo
Keyword(s):  
Paecilomyces Lilacinus ◽  
Purpureocillium Lilacinum ◽  
Meloidogyne Spp

Penelitian ini bertujuan untuk mempelajari pengaruh dosis bionematisida jamur Purpurecilum lilacinum (Syn. Paecilomyces lilacinus) isolat B01TG berbahan pembawa limbah pertanian terhadap keefektifannya untuk mengendalikan serangan nematoda puru akar  Meloidogyne spp.  Percobaan yang berlangsung bulan Februari - Juni 2019 di rumah kaca Fakultas Pertanian Universitas Lampung, menggunakan Rancangan Acak Lengkap (RAL), dengan 5 perlakuan  tingkat dosis bionematisida dan 5 ulangan.  Dosis bionematisida yang diuji yaitu 0 g,  5 g, 10 g, 20 g dan 40 g per tanaman.  Bionematiaida diaplikasikan 3 hari sebelum tanaman tomat ditransplanting.  Satu minggu setelah transplanting, tanaman tomat kemudian  diinfestasi 2000 telur nematoda puru akar.  Data pertumbuhan tanaman yang meliputi tinggi tanaman, jumlah daun, bobot biomassa basah dan produksi, serta tingkat kerusakan akar dianalisis ragam dan dilanjutkan dengan pemisahan nilai tengah menggunakan uji BNT pada taraf nyata 5%.  Hasil penelitian menunjukkan bahwa dosis bionematisida mempengaruhi pertumbuhan tanaman dan kerusakan akar. Pertumbuhan tanaman yang diberi perlakuan 40 g bionematisida lebih baik daripada pertumbuhan tanaman yang diberi  bionematiaida dengan dosis yang lebih rendah. Kerusakan akar tanaman tomat akibat serangan Meloidogyne spp. lebih rendah pada tanaman tomat yang diberikan perlakuan 20 g dan 40 g  daripada kerusakan akar tanaman yang diberi perlakuan bionematisida dengan dosis yang lebih rendah.

scholarly journals PERTUMBUHAN JAMUR Purpureocillium lilacinum (Syn. Paecilomyces lilacinus) ISOLAT TANGGAMUS PADA MEDIA CAMPURAN KULIT UBI UBIKAYU DAN BONGGOL PISANG

2021 ◽  
Vol 9 (2) ◽  
pp. 321
Author(s):  
Oded Saputra ◽  
I Gede Swibawa ◽  
Solikhin Solikhin ◽  
Yuyun Fitriana
Keyword(s):  
Paecilomyces Lilacinus ◽  
Purpureocillium Lilacinum

Limbah pertanian berupa kulit ubi dan bonggol pisang dalam budidaya ubikayu dan pisang di Lampung melimpah. Limbah tersebut  dapat dimanfaatkan sebagai bahan pembawa dalam pembuatan bionematisida berbahan aktif jamur. Penelitian ini bertujuan untuk mempelajari pertumbuhan jamur Purpureocillium lilacinum (Syn. Paecilomyces lilacinus) isolat B01TG berasal dari Tanggamus pada media campuran kulit ubi ubikayu dan bonggol pisang. Penelitian dilaksanakan bulan Januari - Juni 2019 di Laboratorium Bioteknologi, Fakultas Pertanian, Universitas Lampung. Dalam penelitian ini dilakukan dua percobaan yaitu percobaan pertumbuhan jamur pada media ekstrak limbah pertanian dan percobaan pertumbuhan jamur pada media limbah pertanian padat. Limbah pertanian yang dicobakan meliputi bonggol pisang, kulit ubi ubikayu, beras dan kulit udang. Percobaan pertama menggunakan rancangan acak lengkap (RAL), sedangkan percobaan kedua menggunakan rancangan acak kelompok (RAK). Percobaan pertama dan kedua masing-masing terdiri atas 6 perlakuan dan 5 ulangan. Pertumbuhan jamur P. lilacinum (Syn. P. lilacinus) pada media agar dan ekstrak limbah pertanian campuran beras dan kulit udang tertinggi mencapai 84,4%, sedangkan pertumbuhan jamur yang tertinggi pada media padat mencapai 96,4% terjadi pada campuran bonggol pisang, kulit ubi ubikayu, beras dan kulit udang. Kerapatan spora tertinggi mencapai 2,058 x 107 spora/ml pada suspensi pengenceran 10-3 dihasilkan oleh media campuran bonggol pisang, beras dan kulit udang.

scholarly journals Posaconazole in the treatment of refractory Purpureocillium lilacinum (former Paecilomyces lilacinus) keratitis: the salvation when nothing works

2019 ◽  
Vol 12 (4) ◽  
pp. e228645 ◽  
Author(s):  
Mariana Almeida Oliveira ◽  
Analia Carmo ◽  
Andreia Rosa ◽  
Joaquim Murta
Keyword(s):  
Case Report ◽  
Clinical Condition ◽  
Penetrating Keratoplasty ◽  
Contact Lenses ◽  
Clinical Signs ◽  
Paecilomyces Lilacinus ◽  
Purpureocillium Lilacinum ◽  
History Of ◽  
History Of Pain

We report a case of a 41-year-old woman, wearer of contact lenses who was presented to the emergency room with a 2-month history of pain and red eye. She presented with a severe keratitis refractory to quinolones, fortified antibiotics and clotrimazole. Due to the risk of perforation, a tectonic penetrating keratoplasty (PK) was performed. Clinical signs of keratitis recurrence were observed and cultures were positive for Purpureocillium lilacinum (former Paecilomyces lilacinus). The patient did not improve on topical amphotericin B and intracameral voriconazole. Worsening of clinical condition required a new PK. Oral posaconazole was initiated postoperatively and suspended at the fourth postoperative month. The cornea remains clear until the last follow-up visit, 12 months after the second graft. To our knowledge, this is the second case report that documents the effectiveness of oral posaconazole in a refractory P. lilacinus keratitis, resistant to other second-generation triazoles and conventional antifungals.

scholarly journals SELECTION OF INDIGENOUS STRAINS (Paecilomyces lilacinus) PARASITIZE Meloidogyne spp. ISOLATED FROM BA RIA – VUNG TAU PROVINCE

2018 ◽  
Vol 55 (1A) ◽  
pp. 27
Author(s):  
Nguyen Thi Thuy Duong
Keyword(s):  
Forest Soils ◽  
Black Pepper ◽  
Paecilomyces Lilacinus ◽  
Egg Masses ◽  
Parasitizing Female ◽  
Meloidogyne Spp ◽  
Selection Of

Twenty two Paecilomyces lilacinus strains were isolated from forest soils and black pepper rhizospheres in Ba Ria –Vung Tau Province. The ability to degrade chitin of PB 3.3, PB 2.9, QT2, and QT5 strains was high. The ability to degrade casein of PB 1.3, PB 2.10, KL5, and KL6 strains was efficient. And then, these strains were parasitized females and egg masses of Meloidogyne spp. in vitro. In female parasitism test, the rates of parasitizing female nematodes reached more than 50 % after treating for 2 days. Four strains of PB 2.10, PB 1.3, KL6 and QT5 belonged to the first group achieved the highest parasitic (> 90 %) effects on female after 3 days of incubation. In egg masses parasitism test, three strains of PB 1.3, PB 2.10 and QT5 exhibited 83.33 %, 75 % and 75 % parasitism on egg masses after 11 days of incubation. The rates of parasitizing female were higher than egg masses. Three selected strains from the experiments were PB 1.3, PB 2.10 and QT5. 

scholarly journals Effect of Paecilomyces lilacinus on tomato plants and the management of root knot nematodes

2019 ◽  
Vol 17 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Shahriyar Ahmed ◽  
Mohammad Shahjahan Monjil
Keyword(s):  
Plant Pathology ◽  
Plant Growth ◽  
Growth Parameters ◽  
Research Work ◽  
Paecilomyces Lilacinus ◽  
Growth Reduction ◽  
Root Knot Nematodes ◽  
Tomato Plants ◽  
Egg Masses ◽  
Meloidogyne Spp

Effect of Paecilomyces lilacinus on tomato plant growth and the management of root knot nematodes in tomato was studied. The research work was conducted in Microbiology & Bio-control Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh and in Net-house of Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, during the period from July, 2016 to October, 2017. In net-house pot culture experiment, four different treatments were used viz., T1: Inoculation of egg masses (10 egg masses/plant) of Nematodes (Meloidogyne spp.), T2: Application of P. lilacinus, T3: Application of egg masses of Nematodes and P. lilacinus simultaneously, and T4: Control (non-treated). Spore suspension (10×105 Conc.) of P. lilacinus was mixed with the soil before transplantation and Meloidogyne spp. was inoculated on three days after transplantation. Application of P. lilacinus in soil enhanced the plant growth parameters of tomato plants. Inoculation of Meloidogyne spp. reduced plant growth and the reduction was increased with the increase of inoculum density of Meloidogyne spp. Maximum plant growth reduction was recorded when Meloidogyne spp. was inoculated alone. The maximum plant growth was recorded in case of application of P. lilacinus to soil. A high percentage (85%) of egg masses of Meloidogyne spp. was infected by P. lilacinus when applied together J. Bangladesh Agril. Univ. 17(1): 9–13, March 2019

Improving suppression of Meloidogyne spp. by Purpureocillium lilacinum strain 251

Nematology ◽  
2014 ◽  
Vol 16 (6) ◽  
pp. 711-717 ◽  
Author(s):  
Gita Parajuli ◽  
Gita Parajuli ◽  
Robert Kemerait ◽  
Gita Parajuli ◽  
Robert Kemerait ◽  
...  
Keyword(s):  
Cover Crops ◽  
Soil Surface ◽  
Bare Soil ◽  
Parasitic Nematodes ◽  
Plant Parasitic Nematodes ◽  
Crimson Clover ◽  
Purpureocillium Lilacinum ◽  
Soil Temperatures ◽  
Meloidogyne Spp ◽  
Nematode Eggs

The fungus Purpureocillium lilacinum (syn. Paecilomyces lilacinus) is marketed for control of plant-parasitic nematodes in several countries. Our objectives in this study were to determine whether suppression of Meloidogyne spp. by P. lilacinum strain 251 was affected by the crop plant and whether growing winter cover crops such as rye and crimson clover would improve suppression of M. incognita on cotton by the fungus. All experiments were conducted in a glasshouse using non-sterilised field soil. To determine the relative efficacy of P. lilacinum on cotton (Gossypium hirsutum), peanut (Arachis hypogaea) and maize (Zea mays), four concentrations of P. lilacinum (NemOut™) were applied in furrow: 336, 252, 168 and 0 g ha−1. Although the fungus reduced numbers of eggs of Meloidogyne spp. on all crops, percentage suppression was lower on maize than on cotton and peanut at all inoculum levels of P. lilacinum. When rye and crimson clover were grown in pots for 30 days and then killed with a herbicide prior to applying P. lilacinum and M. incognita and planting cotton, the fungus failed to suppress numbers of nematode eggs when the surface residues of the cover crops were removed. However, when the residues were left on the soil surface, percentage suppression (49% for clover and 63% for rye) was greater than when the soil was left fallow (36%). The residues could have created conditions that were more conducive than bare soil to the fungus, such as lower soil temperatures and increase moisture retention.

scholarly journals Efeitos da temperatura sobre a atividade de fungos no controle biológico de Meloidogyne javanica E M. incognita raça 3

2003 ◽  
Vol 27 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Fábio R. Alves ◽  
Vicente Paulo Campos
Keyword(s):  
Meloidogyne Javanica ◽  
Paecilomyces Lilacinus ◽  
Duddingtonia Flagrans ◽  
Paecilomyces Variotii ◽  
Meloidogyne Spp ◽  
Arthrobotrys Conoides

O efeito de Arthrobotrys conoides, Duddingtonia flagrans, Paecilomyces lilacinus, Paecilomyces variotii, Monacrosporium doedycoides e um isolado de rizobactéria na reprodução e crescimento populacional de Meloidogyne javanica e M. incognita raça 3 em tomateiro Santa Clara, suscetível à Meloidogyne spp., foi estudado em três ambientes distintos: 1) casa-de-vegetação sem controle de temperatura; 2) sala climatizada com temperatura do ar constante a 24ºC; 3) em banho-maria com temperatura do solo mantida em 29-30ºC, colocado na mesma sala climatizada caracterizada anteriormente. Maior crescimento populacional de M. javanica e de M. incognita raça 3 ocorreu em solo aquecido, comparado com aquela em casa-de-vegetação e sala climatizada, e o número de galhas causado por M. javanica e M. incognita raça 3 foi maior em solo aquecido e em sala climatizada do que em casa-de-vegetação. Em solo aquecido e em sala climatizada, A. conoides e a rizobactéria reduziram (P< 0,05) o número de galhas de M. incognita raça 3, comparado com a testemunha. Em solo aquecido, A. conoides reduziu o número de ovos de M. incognita raça 3, comparado com a testemunha. Em casa-de-vegetação, todos os antagonistas reduziram o número de ovos de M. incognita raça 3, comparado com a testemunha. Em casa-de-vegetação, todos os antagonistas reduziram o número de ovos em relação à testemunha.

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