scholarly journals Efeitos da temperatura sobre a atividade de fungos no controle biológico de Meloidogyne javanica E M. incognita raça 3

2003 ◽  
Vol 27 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Fábio R. Alves ◽  
Vicente Paulo Campos
Keyword(s):  
Meloidogyne Javanica ◽  
Paecilomyces Lilacinus ◽  
Duddingtonia Flagrans ◽  
Paecilomyces Variotii ◽  
Meloidogyne Spp ◽  
Arthrobotrys Conoides

O efeito de Arthrobotrys conoides, Duddingtonia flagrans, Paecilomyces lilacinus, Paecilomyces variotii, Monacrosporium doedycoides e um isolado de rizobactéria na reprodução e crescimento populacional de Meloidogyne javanica e M. incognita raça 3 em tomateiro Santa Clara, suscetível à Meloidogyne spp., foi estudado em três ambientes distintos: 1) casa-de-vegetação sem controle de temperatura; 2) sala climatizada com temperatura do ar constante a 24ºC; 3) em banho-maria com temperatura do solo mantida em 29-30ºC, colocado na mesma sala climatizada caracterizada anteriormente. Maior crescimento populacional de M. javanica e de M. incognita raça 3 ocorreu em solo aquecido, comparado com aquela em casa-de-vegetação e sala climatizada, e o número de galhas causado por M. javanica e M. incognita raça 3 foi maior em solo aquecido e em sala climatizada do que em casa-de-vegetação. Em solo aquecido e em sala climatizada, A. conoides e a rizobactéria reduziram (P< 0,05) o número de galhas de M. incognita raça 3, comparado com a testemunha. Em solo aquecido, A. conoides reduziu o número de ovos de M. incognita raça 3, comparado com a testemunha. Em casa-de-vegetação, todos os antagonistas reduziram o número de ovos de M. incognita raça 3, comparado com a testemunha. Em casa-de-vegetação, todos os antagonistas reduziram o número de ovos em relação à testemunha.

Association of Duddingtonia flagrans with Microorganisms for Management of Meloidogyne javanica and Acquisition of Nutrients in Soybean

2021 ◽  
pp. 104626
Author(s):  
Huarlen Marcio Balbino ◽  
Thalita Suelen Avelar Monteiro ◽  
Raul Rodrigues Coutinho ◽  
Paulo Victor Magalhães Pacheco ◽  
Leandro Grassi de Freitas
Keyword(s):  
Meloidogyne Javanica ◽  
Duddingtonia Flagrans

scholarly journals Τhe Nematicidal Potential of Bioactive Streptomyces Strains Isolated from Greek Rhizosphere Soils Tested on Arabidopsis Plants of Varying Susceptibility to Meloidogyne spp.

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 699
Author(s):  
Christianna Meidani ◽  
Alexandros Savvidis ◽  
Evaggelia Lampropoulou ◽  
Aggeliki Sagia ◽  
Efstathios Katsifas ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Arabidopsis Thaliana ◽  
Meloidogyne Incognita ◽  
Meloidogyne Javanica ◽  
Wild Type ◽  
Biological Cycle ◽  
Second Stage ◽  
Cycle Arrest ◽  
Meloidogyne Spp ◽  
Culture Supernatants

A total of 461 indigenous Streptomycetes strains recovered from various Greek rhizosphere habitats were tested for their bioactivity. All isolates were examined for their ability to suppress the growth of 12 specific target microorganisms. Twenty-six were found to exert antimicrobial activity and were screened for potential nematicidal action. S. monomycini ATHUBA 220, S. colombiensis ATHUBA 438, S. colombiensis ATHUBA 431, and S. youssoufensis ATHUBA 546 were proved to have a nematicidal effect and thus were further sequenced. Batch culture supernatants and solvent extracts were assessed for paralysis on Meloidogyne javanica and Meloidogyne incognita second-stage juveniles (J2). The solvent extracts of S. monomycini ATHUBA 220 and S. colombiensis ATHUBA 438 had the highest paralysis rates, so these Streptomycetes strains were further on tested for nematodes’ biological cycle arrest on two Arabidopsis thaliana plants; the wild type (Col-0) and the katanin mutant fra2, which is susceptible to M. incognita. Interestingly, S. monomycini ATHUBA 220 and S. colombiensis ATHUBA 438 were able to negatively affect the M. incognita biological cycle in Col-0 and fra2 respectively, and increased growth in Col-0 upon M. incognita infection. However, they were ineffective against M. javanica. Fra2 plants were also proved susceptible to M. javanica infestation, with a reduced growth upon treatments with the Streptomyces strains. The nematicidal action and the plant-growth modulating abilities of the selected Streptomycetes strains are discussed.

Infection of Meloidogyne javanica by Paecilomyces lilacinus

Nematology ◽  
1999 ◽  
Vol 1 (2) ◽  
pp. 131-139 ◽  
Author(s):  
Rita Holland ◽  
Keith Williams ◽  
Alamgir Khan
Keyword(s):  
Body Wall ◽  
Meloidogyne Javanica ◽  
The Body ◽  
Paecilomyces Lilacinus ◽  
Adult Females ◽  
In Vitro Interaction

AbstractThe in vitro interaction of Paecilomyces lilacinus strain 251 with eggs, 3rd and 4th stage juveniles and adult females of Meloidogyne javanica was studied. Eggs of all stages, including those containing unhatched juveniles, were infected by P. lilacinus. Infection of eggs occurred following flattening of hyphae to the egg surface and formation of appressoria. Sometimes these occurred within extensive networks of hyphae of the egg surface. Hyphae later grew out of the egg to continue growing or form conidiophores. Third and 4th stage juveniles and adult females were readily infected, with hyphae and conidiophores penetrating the body wall. Die Infektion von Meloidogyne javanica durch Paecilomyces lilacinus - Es wurden die in vitro auftretenden Wechselwirkungen zwischen Paecilomyces lilacinus Stamm 251 und den Eiern, J3, J4 und adulten Weibchen von Meloidogyne javanica untersucht. Eier wurden in allen Stadien von P. lilacinus infiziert einschliesslich der ungeschlupfte J2 enthaltenden Eier. Die Infektion erfolgte anschliessend an eine Abflachung von Hyphen auf der Eioberflache und eine Appressorienbildung. Manchmal erschienen diese innerhalb eines ausgedehnten Netzwerkes von Hyphen auf der Eioberflache. Spater wuchsen Hyphen aus dem Ei heraus, wuchsen weiter oder bildeten Konidiophoren. Juvenile des dritten oder vierten Stadiums und adulte Weibchen wurden ohne weiteres befallen, wobei Hyphen und Konidiophoren durch die Korperwand drangen.

scholarly journals Heterologous Expression of the Mi-1.2 Gene from Tomato Confers Resistance Against Nematodes but Not Aphids in Eggplant

2006 ◽  
Vol 19 (4) ◽  
pp. 383-388 ◽  
Author(s):  
Fiona L. Goggin ◽  
Lingling Jia ◽  
Gowri Shah ◽  
Stephanie Hebert ◽  
Valerie M. Williamson ◽  
...  
Keyword(s):  
Genetic Background ◽  
Meloidogyne Javanica ◽  
Nematode Resistance ◽  
Macrosiphum Euphorbiae ◽  
Leucine Rich Repeat ◽  
Root Knot Nematode ◽  
Potato Aphid ◽  
Tomato Line ◽  
Meloidogyne Spp ◽  
Susceptible Tomato

The Mi-1.2 gene in tomato (Solanum lycopersicum) is a member of the nucleotide-binding leucine-rich repeat (NB-LRR) class of plant resistance genes, and confers resistance against root-knot nematodes (Meloidogyne spp.), the potato aphid (Macrosiphum euphorbiae), and the sweet potato whitefly (Bemisia tabaci). Mi-1.2 mediates a rapid local defensive response at the site of infection, although the signaling and defensive pathways required for resistance are largely unknown. In this study, eggplant (S. melongena) was transformed with Mi-1.2 to determine whether this gene can function in a genetic background other than tomato. Eggplants that carried Mi-1.2 displayed resistance to the root-knot nematode Meloidogyne javanica but were fully susceptible to the potato aphid, whereas a susceptible tomato line transformed with the same transgene was resistant to nematodes and aphids. This study shows that Mi-1.2 can confer nematode resistance in another Solanaceous species. It also indicates that the requirements for Mi-mediated aphid and nematode resistance differ. Potentially, aphid resistance requires additional genes that are not conserved between tomato and eggplant.

scholarly journals Resistência de porta-enxertos para pessegueiro e ameixeira aos nematóides causadores de galhas (Meloidogyne spp.)

2000 ◽  
Vol 30 (1) ◽  
pp. 69-72 ◽  
Author(s):  
José Carlos Fachinello ◽  
Carlos Augusto Posser Silva ◽  
Cesar Sperandio ◽  
Alexandre Couto Rodrigues ◽  
Eder Zarnott Strelow
Keyword(s):  
Meloidogyne Incognita ◽  
Meloidogyne Javanica ◽  
Meloidogyne Spp

O trabalho foi realizado com o objetivo de comparar o comportamento de cinco diferentes porta-enxertos para frutas de caroço em frente a duas espécies de nematóides do gênero Meloidogyne (Meloidogyne javanica e Meloidogyne incognita). O cultivar GF 677 foi obtido a partir do cultivo in vitro e os demais porta-enxertos a partir de sementes. Aos dois meses, as plântulas foram repicadas e inoculadas com uma mistura de Meloidogyne javanica e M. incognita aos 30, 60 e 70 dias após o plantio em canteiros incorporando-se 0,2kg de solo altamente infestado com os nematóides ao redor de cada planta. Ao final do experimento, avaliaram-se o desenvolvimento das plantas e o grau de infecção de cada cultivar. O grau de resistência dos cultivares foi estimado a partir do índice de galhas, obtido através de uma escala de grau ou nota, a qual varia de 0 a 5, em função do número de galhas ou ootecas. Os resultados obtidos demonstraram que o cultivar Okinawa não apresentou galhas no sistema radicular e na análise de crescimento foi superior aos demais cultivares. Os cultivares R-15-2 e Aldrighi foram considerados resistentes aos fitonematóides por apresentarem pequeno número de galhas no sistema radicular. Já o cultivar GF 677 apresentou maior número de galhas no sistema radicular, chegando a 126 galhas/g de raiz.

scholarly journals Heritability of Root-knot Nematode (Meloidogyne spp.) Resistance in Sweetpotatoes

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 622a-622 ◽  
Author(s):  
W. R. Maluf ◽  
S. M. Azevedo ◽  
V.P. Campos
Keyword(s):  
Meloidogyne Incognita ◽  
Meloidogyne Javanica ◽  
Broad Sense Heritability ◽  
Root Knot Nematode ◽  
Egg Masses ◽  
Substrate Removal ◽  
Meloidogyne Spp ◽  
Diverse Origin ◽  
Race 2 ◽  
Resistant Genotypes

Heritabilities for resistance to root knot nematodes (Meloidogyne javanica and Meloidogyne incognita races 1, 2, 3, and 4) were studied in a population of 226 sweetpotato clones of diverse origin. For each nematode isolate tested, 128-cell speedling trays were filled with previously inoculated substrate (30000 eggs/1000 mL substrate). Sweetpotato clones suitably tagged and identified were randomly planted in the cells (one plant/cell), with a total of four plants per clone per isolate. Ninety days after inoculation, sweetpotato plants had their roots washed for substrate removal, and treated with 150 mg·L–1 Phloxine B to stain nematode egg masses. The number of egg masses per root was recorded, and plants were accordingly assigned scores from 0 (highly resistant) to 5 (highly susceptible). Broad-sense heritability estimates were 0.87, 0.91, 0.81, 0.95, and 0.93 respectively for resistance to M. javanica and races 1, 2, 3, and 4 of M. incognita. The frequencies of resistant genotypes were higher for M. javanica and lower for M. incognita race 2. Genotypic correlations (rG) among the resistances to the various Meloidogyne isolates utilized were weak, ranging from 0.11 to 0.57, suggesting independent genetic controls. Clones could be selected, however, with high levels of resistance to all nematode isolates tested. (This work was supported by CNPq, CAPES, FAPEMIG, and FAEPE/UFLA.)

scholarly journals Meloidogyne Species Associated with Weeds in Rio Grande do Sul

2019 ◽  
Vol 37 ◽  
Author(s):  
C. BELLÉ ◽  
T.E. KASPARY ◽  
R.R. BALARDIN ◽  
R.F. RAMOS ◽  
Z.I. ANTONIOLLI
Keyword(s):  
Host Plants ◽  
Management Practices ◽  
Rio Grande ◽  
Meloidogyne Javanica ◽  
Rio Grande Do Sul ◽  
Weed Species ◽  
Root Knot Nematode ◽  
Root Knot Nematodes ◽  
Meloidogyne Spp ◽  
Meloidogyne Species

ABSTRACT: The frequency of species of root-knot nematodes (Meloidogyne spp.) was evaluated in weeds collected in different fallow farms in the State of Rio Grande do Sul, Brazil. In the samples where the nematode was found, the species of the root-knot nematode was identified by electrophoresis using the isozyme esterase. They were obtained from weeds belonging to 24 weed species from 13 different botanical families: Amaranthaceae, Asteraceae, Commelinaceae, Convovulaceae, Cyperaceae, Euphorbiaceae, Lamiaceae, Malvaceae, Oxalidaceae, Poaceae, Portulacaceae, Solanaceae, Verbenaceae. Meloidogyne javanica Est J3 (Rm: 1.0, 1.25, 1.40) was the most frequent species and occurred in 53.3% of the samples. M. arenaria with phenotype Est. A2 (Rm: 1.20, 1.30) was detected in 15.6% of the samples. M. incognita Est. I2 (Rm: 1.0, 1.1), M. ethiopica Est. E3 (Rm: 0.9, 1.15, 1.30), M. enterolobii Est. M2 (Rm: 0.7, 0.75, 0.9, 0.95) and M. hapla Est. H1 (Rm: 1.17) in 13.3%, 8.9%, 6.7% and 2.2% of the samples, respectively. Therefore, knowledge of the range of host plants to different species of the root-knot nematode can positively contribute to the adoption of management practices that allow the reduction of their populations in the soil.

scholarly journals Occurrence of Meloidogyne spp. in Cerrado Vegetations and Reaction of Native Plants to Meloidogyne javanica

2013 ◽  
Vol 162 (7-8) ◽  
pp. 449-455 ◽  
Author(s):  
Joelma G. P. Silva ◽  
Cleber Furlanetto ◽  
Maria R. A. Almeida ◽  
David B. Rocha ◽  
Vanessa S. Mattos ◽  
...  
Keyword(s):  
Native Plants ◽  
Meloidogyne Javanica ◽  
Meloidogyne Spp
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