In vitro Propagation of Nothofagus obliqua (Fagaceae).

1995 ◽  
Vol 43 (6) ◽  
pp. 601 ◽  
Author(s):  
Pastur GJ Martinez ◽  
ME Arena
Keyword(s):  
In Vitro Propagation ◽  
Shoot Culture ◽  
Shoot Multiplication ◽  
Culture Conditions ◽  
Multiplication Rate ◽  
Nothofagus Obliqua ◽  
Low Salt ◽  
Broadleaved Tree ◽  
Induction Stage

Suitable culture conditions were investigated for in vitro propagation through shoot culture of seedlings and the acclimatization of the rooted plantlets of Nothofagus obliqua. Explant size and leaf expansion were critical for culture initiation. A medium with a low salt concentration and the addition of L-glutamine, as broadleaved tree medium, was suitable for shoot multiplication and it was influenced by both cytokinin and auxin. A multiplication rate of x5 has been obtained with 0.55 micro M benzyladenine (BA), and 0.09 micro M BA and 0.09 micro M IBA at day 63. Rooted shoots (51.9%) were obtained with 0.61 IBA and 1 week darkness at the induction stage. Rooted plants were then successfully acclimatised and 75% survival rate was obtained after 4 months.

scholarly journals In vitro propagation of Rosa ‘Konstancin’ (R. rugosa × R. beggeriana), a plant with high nutritional and pro-health value

2018 ◽  
Vol 30 (2) ◽  
pp. 259-267 ◽  
Author(s):  
Agnieszka Wojtania ◽  
Bożena Matysiak
Keyword(s):  
In Vitro Propagation ◽  
Photosynthetic Activity ◽  
Shoot Culture ◽  
Bud Break ◽  
Multiplication Rate ◽  
Shoot Cultures ◽  
Old Field ◽  
Health Value

Abstract The aim of the study was to develop an efficient micropropagation system for Rosa ‘Konstancin’, an interspecific hybrid between R. rugosa and R. beggeriana, whose fruits have high pro-health value. Shoot cultures were initiated from shoot buds collected in May and August from 15-year-old field-grown Rosa ‘Konstancin’ shrubs. The effect and interaction of different concentrations of phytohormones, sucrose and iron sources on in vitro initiation, multiplication and rooting of shoots were studied. The time of collecting explants from donor plants significantly affected the initiation of shoot culture of Rosa ‘Konstancin’. Considerably higher frequency of bud break (100%) was obtained in explants isolated in August as compared to those collected at the end of May (30%). All buds developed into single shoots after 2-4 weeks of growing on the basal Murashige and Skoog medium containing 2.2 µM BAP, 0.3 µM GA3 and 88 mM of sucrose. The highest multiplication rate (4.8 shoots/explant) in a 5-week period was obtained on MS medium containing 50% of nitrogen salts, 3.1 µM BAP, 0.9 µM GA3 and 58 mM sucrose. High rooting frequency (100%) and quality of rooted plantlets was obtained on a medium containing 0.5 µM IBA, 138 µM Fe-EDDHA and 88 mM sucrose. Fe-EDDHA had a beneficial effect on the growth and photosynthetic activity of Rosa ‘Konstancin’ plantlets, which were successfully acclimatized ex vitro, with a more than 90% survival rate.

scholarly journals OPTIMASI NAA DAN BAP TERHADAP PERTUMBUHAN DAN PERKEMBANGAN TUNAS MIKRO TANAMAN KANTONG SEMAR (Nepenthes mirabilis) SECARA IN VITRO

2015 ◽  
Vol 5 (2) ◽  
pp. 29
Author(s):  
ROSMAINA ROSMAINA ◽  
DINNI ARYANI
Keyword(s):  
In Vitro Culture ◽  
Research Design ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Alternative Method ◽  
Shoot Number ◽  
Root Explant ◽  
Induction Stage

Conventional propagation of Nepenthes was difficult to do. To overcome the problems were required alternative method such as in vitro propagation. The objective of this research was to obtain the best treatment of BAP + NAA on shoot multiplication of Nepenthes through in vitro culture. The research design used Randomized Completely Design consist of seven treatments, e.g. 1) ½ MS0 (control); 2) ½ MS + 1 ppm BAP + 0.5 ppm NAA; 3) ½ MS + 1 ppm BAP + 1 ppm NAA; 4) ½ MS + 1.5 ppm BAP + 0.5 ppm NAA; 5) ½ MS + 1.5 ppm BAP + 1 ppm NAA; 6) ½ MS + 2 ppm BAP + 0.5 ppm NAA dan 7) ½ MS + 2 ppm BAP + 1 ppm NAA. The parameter observed were number of shoot, number of nodul, number of leafs, number of pitcher and number of root. The result of this research showed that treatment of ½ MS + 1 ppm BAP + 1 ppm NAA is the best treatment compared to others. At induction stage, this treatment can produce the number of shoot, number of nodul, and number of root were 1.6 shoots/explant, 10.8 nodul/explant and 3.6 root/explant, respectively. At subculture, this treatment can produce the number of shoot, number of leafs, and number of pitcher were 5.8 shoots/explant, 12.4 leafs/explant and 5.2 pitcher/explant, respectively.

scholarly journals Perbanyakan in vitro tanaman kina (Cinchona ledgeriana Moens) melalui tunas aksiler dan apikal In vitro propagation of cinchona (Cinchona ledgeriana Moens) from axillary and apical buds

2016 ◽  
Vol 77 (1) ◽  
Author(s):  
Imron RIYADI ◽  
J. S. TAHARDI TAHARDI
Keyword(s):  
In Vitro Propagation ◽  
Shoot Growth ◽  
Shoot Multiplication ◽  
Shoot Length ◽  
Multiplication Rate ◽  
Subsequent Growth ◽  
Apical Buds ◽  
Murashige Skoog ◽  
Cinchona Ledgeriana

AbstractThe use of the appropriate source of nodalbud explants in culture can increase theeffectiveness and efficiency of shootmultiplication. An experiment was conducted todetermine and compare the rate of in vitro shootmultiplication from apical and axillary budsin cinchona (Cinchona ledgeriana Moens) andtheir subsequent growth and development. Theplant material used was Cinchona ledgerianaoriginating from the Indonesian Tea andCinchona Research Institute, Gambung, WestJava. Explants were taken from apical andaxillary nodes from in vitro germinated seedlings.The cultures were incubated at 26 0 C and 60%relative humidity under a 14-h photoperiod withlight intensity of 30 µmol photon/m 2 /sec.provided by cool-white fluorescent tubes (TL40 W) for 4 - 8 weeks. The parameters observedwere shoot multiplication rate, shoot growth anddevelopment such as shoot length, leaf numberand rooting frequency. Apical and axillary nodesproduced shoots at different multiplication rateson Murashige-Skoog (MS) standard mediumcontaining 30 g/L sucrose and supplemented with1 – 5 mg/L BA in combination with 0.1 mg/L IBA.Furthermore, shoots or plantlets of cinchonagrew and developed on the same mediacontaining 5 – 10 mg/L IAA combined with0.5 mg/L IBA. The results showed that shootmultiplication rate was higher in axillary than inapical nodes. The highest multiplication rate inaxillary nodes was 24.6 shootlets with 3 mg/LBA treatment, whereas in apical nodes it was17.2 shootlets with 5 mg/L BA treatment for eightweeks. The highest rooting frequency ofcinchona plantlet was 90%, achieved with 5 mg/LIAA in combination with 0.5 mg/L IBA. Theplantlets were successfully acclimatized andtransplanted to the fieldAbstrakSumber eksplan berupa nodus/tunas padakultur in vitro umum digunakan untuk multi-plikasi tunas. Penelitian ini bertujuan untukmembandingkan tingkat multiplikasi antara tunasapikal dengan tunas aksiler tanaman kina Ledgersecara in vitro. Bahan tanaman yang digunakanadalah kina Ledger (Cinchona ledgeriana Moens)yang berasal dari Pusat Penelitian Teh dan Kina,Gambung, Jawa Barat. Eksplan berupa nodus/tunas apikal dan aksiler asal biji yang dikecam-bahkan secara in vitro. Kultur tersebut diinku-basikan dalam ruang terang pada intensitascahaya 30 μmol foton/m 2 /detik dengan periodepenyinaran 14 jam pada suhu 260 C dankelembaban relatif + 60% selama 4 – 8 minggu.Parameter yang diamati adalah perbandinganmultiplikasi tunas dan pertumbuhan tunas yangmeliputi rata-rata tinggi tunas, jumlah daun danfrekuensi pengakaran. Nodus apikal maupunaksiler menghasilkan tunas dengan tingkatMurashige-Skoog (MS) standar yang me-ngandung sukrosa30 g/L dan ditambahkan BA1 – 5 mg/L dikombinasikan IBA 0,1 mg/L.Selanjutnya tunas/planlet kina tersebut berhasiltumbuhdan berkembang pada medium sama yangdiberi IAA 5 – 10 mg/L dikombinasikan denganIBA 0,5 mg/L. Hasil penelitian menunjukkanbahwa tingkat multiplikasi tunas aksiler lebihtinggi dari pada tunas apikal. Multiplikasi tunasaksiler menghasilkan jumlah tunas rata-ratatertinggi sebesar 24,6 tunas per eksplan padaperlakuan BA 3 mg/L sedangkan multiplikasitunas apikal tertinggi sebesar 17,2 tunas pereksplan pada perlakuan BA 5 mg/L pada umurdelapan minggu. Frekuensi pengakaran planletkina tertinggi mencapai 90% pada perlakuan IAA10 mg/L yang dikombinasikan dengan IBA 0,5mg/L. Planlet yang dihasilkan telah berhasildiaklimatisasi dan dipindahkan ke tempatpersemaian lapang.

scholarly journals Perbanyakan in vitro tanaman kina (Cinchona ledgeriana Moens) melalui tunas aksiler dan apikal In vitro propagation of cinchona (Cinchona ledgeriana Moens) from axillary and apical buds

2016 ◽  
Vol 77 (1) ◽  
Author(s):  
Imron RIYADI ◽  
J. S. TAHARDI TAHARDI
Keyword(s):  
In Vitro Propagation ◽  
Shoot Growth ◽  
Shoot Multiplication ◽  
Shoot Length ◽  
Multiplication Rate ◽  
Subsequent Growth ◽  
Apical Buds ◽  
Murashige Skoog ◽  
Cinchona Ledgeriana

AbstractThe use of the appropriate source of nodalbud explants in culture can increase theeffectiveness and efficiency of shootmultiplication. An experiment was conducted todetermine and compare the rate of in vitro shootmultiplication from apical and axillary budsin cinchona (Cinchona ledgeriana Moens) andtheir subsequent growth and development. Theplant material used was Cinchona ledgerianaoriginating from the Indonesian Tea andCinchona Research Institute, Gambung, WestJava. Explants were taken from apical andaxillary nodes from in vitro germinated seedlings.The cultures were incubated at 26 0 C and 60%relative humidity under a 14-h photoperiod withlight intensity of 30 µmol photon/m 2 /sec.provided by cool-white fluorescent tubes (TL40 W) for 4 - 8 weeks. The parameters observedwere shoot multiplication rate, shoot growth anddevelopment such as shoot length, leaf numberand rooting frequency. Apical and axillary nodesproduced shoots at different multiplication rateson Murashige-Skoog (MS) standard mediumcontaining 30 g/L sucrose and supplemented with1 – 5 mg/L BA in combination with 0.1 mg/L IBA.Furthermore, shoots or plantlets of cinchonagrew and developed on the same mediacontaining 5 – 10 mg/L IAA combined with0.5 mg/L IBA. The results showed that shootmultiplication rate was higher in axillary than inapical nodes. The highest multiplication rate inaxillary nodes was 24.6 shootlets with 3 mg/LBA treatment, whereas in apical nodes it was17.2 shootlets with 5 mg/L BA treatment for eightweeks. The highest rooting frequency ofcinchona plantlet was 90%, achieved with 5 mg/LIAA in combination with 0.5 mg/L IBA. Theplantlets were successfully acclimatized andtransplanted to the fieldAbstrakSumber eksplan berupa nodus/tunas padakultur in vitro umum digunakan untuk multi-plikasi tunas. Penelitian ini bertujuan untukmembandingkan tingkat multiplikasi antara tunasapikal dengan tunas aksiler tanaman kina Ledgersecara in vitro. Bahan tanaman yang digunakanadalah kina Ledger (Cinchona ledgeriana Moens)yang berasal dari Pusat Penelitian Teh dan Kina,Gambung, Jawa Barat. Eksplan berupa nodus/tunas apikal dan aksiler asal biji yang dikecam-bahkan secara in vitro. Kultur tersebut diinku-basikan dalam ruang terang pada intensitascahaya 30 μmol foton/m 2 /detik dengan periodepenyinaran 14 jam pada suhu 260 C dankelembaban relatif + 60% selama 4 – 8 minggu.Parameter yang diamati adalah perbandinganmultiplikasi tunas dan pertumbuhan tunas yangmeliputi rata-rata tinggi tunas, jumlah daun danfrekuensi pengakaran. Nodus apikal maupunaksiler menghasilkan tunas dengan tingkatMurashige-Skoog (MS) standar yang me-ngandung sukrosa30 g/L dan ditambahkan BA1 – 5 mg/L dikombinasikan IBA 0,1 mg/L.Selanjutnya tunas/planlet kina tersebut berhasiltumbuhdan berkembang pada medium sama yangdiberi IAA 5 – 10 mg/L dikombinasikan denganIBA 0,5 mg/L. Hasil penelitian menunjukkanbahwa tingkat multiplikasi tunas aksiler lebihtinggi dari pada tunas apikal. Multiplikasi tunasaksiler menghasilkan jumlah tunas rata-ratatertinggi sebesar 24,6 tunas per eksplan padaperlakuan BA 3 mg/L sedangkan multiplikasitunas apikal tertinggi sebesar 17,2 tunas pereksplan pada perlakuan BA 5 mg/L pada umurdelapan minggu. Frekuensi pengakaran planletkina tertinggi mencapai 90% pada perlakuan IAA10 mg/L yang dikombinasikan dengan IBA 0,5mg/L. Planlet yang dihasilkan telah berhasildiaklimatisasi dan dipindahkan ke tempatpersemaian lapang.

scholarly journals Establishment of a Rapid Plant Regeneration System in Physalis angulata L. through Axillary Meristems

2015 ◽  
Vol 7 (4) ◽  
pp. 471-474
Author(s):  
Owk ANIEL KUMAR ◽  
Songa RAMESH ◽  
Sape SUBBA TATA
Keyword(s):  
Shoot Culture ◽  
Shoot Multiplication ◽  
Multiple Shoot ◽  
Herbaceous Plant ◽  
Axillary Meristem ◽  
Regeneration System ◽  
Axillary Meristems ◽  
Meristem Explants ◽  
Physalis Angulata

An optimal plant propagation method of Physalis angulata L., a medicinally important herbaceous plant species has been developed using axillary meristem explants. Shoot bud proliferation was initiated from axillary meristem explants cultured on MS medium supplemented with various concentrations of 0.5-2.5mg/L/(BAP)/(Zeatin)/(KIN). The maximum in vitro response of shooting frequency of explants (88.1%) and shoots per explant (42) was achieved with medium containing 1.0mg/L BAP. Multiple shoot culture was established by repeated subculturing of the shoot buds of axillary meristems on shoot multiplication medium. Among the subculture media BAP in combination with 1.5mg/L (IAA)+0.25mg/L(GA3) produced maximum shoots per explant (128±0.29) after two weeks of culture. Effective in vitro shoot elongation and rooting was achieved on 1.0mg/L(GA3) and 1.0mg/L(IBA), respectively. Most of the generated shoots were successfully transferred to soil under field conditions. The survival percentage of the transferred plants on soil was found to be 90 per cent.  This protocol can be used for commercial propagation and for future genetic improvement studies.

scholarly journals Assessment of In-vitro culture through Nodal explants of Dendrocalamus hamiltonii

2021 ◽  
Vol 2 (1) ◽  
pp. 130-133
Author(s):  
Abha Jha ◽  
◽  
Sunila Das ◽  
Keyword(s):  
Experimental Study ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Shoot Multiplication ◽  
Growth Hormones ◽  
Nodal Explants ◽  
Bud Break ◽  
Multiplication Rate ◽  
Dendrocalamus Hamiltonii

The present experimental study was aimed to overcome the traditional methods of propagation that limit the number of propagules by in-vitro regeneration through nodal explants of Dendrocalamus hamiltonii with a comparative study of growth regulators during the shooting and rooting process. Dendrocalamus hamiltonii is distributed from the Himalayas (Nepal) to the northern part of Burma. Collection of explants was done from different selected sites of CPTs. There was the use of HgCl2 and Ca (OCl)2 as sterilizing agents in different concentrations and its effect was visualized during the sprouting stage. Culm explants were cultured in a bottle containing White media (Wm) supplemented with BA and Kinetin for sprouting and IAA, IBA, NAA for rooting. There is also the use of IAA+IBA+NAA in combined form as a supplementary solution 0.1% HgCl2 treatment for 20-minute results into77.80% aseptic buds and 72% bud -break. Among the used growth-hormones, BA with concentration 0.25mg/l and 0.50mg/l respectively were appropriate for shoot-multiplication rate, 4.01±0.3 and 4.3±0.4 were ideal observation incorporation with BA (1.00mg/l) and BA (1.50mg/l) respectively. Maximum sprouting rate14.77±3.37with application of BA (2.00mg/l) and maximum shoot length4.3±0.4 is observed at BA (1.50mg/l). The applications of rooting hormone IAA+IBA+NAA in the concentration of 1.0 mg/l results in 72.5±0.3(rooting) and 11.1±0.3 (av. No. of the root).

scholarly journals An efficient in vitro propagation protocol for snowdrop anemone (Anemone sylvestris L.) 

2017 ◽  
Vol 44 (No. 4) ◽  
pp. 186-194 ◽  
Author(s):  
Jana Šedivá ◽  
Pavla Zahumenická ◽  
Eloy Fernández Cusimamani
Keyword(s):  
Plant Growth ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Growth Characteristics ◽  
Root Induction ◽  
Shoot Induction ◽  
Free Medium ◽  
In Vitro Production ◽  
Vitro Production

This study investigated in vitro production of diploid (AS2) and tetraploid (AS4) cytotypes of snowdrop anemone. The effect of plant growth regulators (PGRs) on in vitro shoot multiplication and rooting was investigated. The effect of activated charcoal (AC) on root induction was also studied. Ploidy level affected growth characteristics during multiplication and rooting. Shoot induction in AS4 was higher on medium supplemented with cytokinin (3.2–3.6), while the AS2 clone formed the most shoots on PGR-free medium (3.6). The highest rooting percentage was achieved on PGR-free medium in both genotypes (AS2 clone, 100% and AS4 clone, 93.3%). The addition of AC to the PGR media largely increased root induction and root length. Rooted plantlets were successfully acclimatised in the greenhouse with 100% survival. Thus, the described micropropagation protocol represents a rapid and effective in vitro propagation method for utilisation in horticulture and conservation programmes of snowdrop anemone.

High in vitro shoot proliferation in the apple cultivar Jonagold induced by benzyladenine analogues

2002 ◽  
Vol 50 (2) ◽  
pp. 191-195 ◽  
Author(s):  
K. Magyar-Tábori ◽  
J. Dobránszki ◽  
E. Jámbor-Benczúr
Keyword(s):  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Length ◽  
Multiplication Rate ◽  
Apple Cultivar ◽  
High Concentration ◽  
Suppression Effect ◽  
High Concentrations ◽  
In Vitro Shoot Proliferation

The in vitro shoot multiplication of apple cv. Jonagold was tested on media containing benzyladenine, benzyladenine riboside or meta-topolin in different concentrations (from 0.0 to 5.0 mg l-1). The optimal concentration for the best multiplication varied according to the type of cytokinin. The highest multiplication rate (on average 6.9 and 5.9 new shoots per explant) was achieved using 5.0 mg l-1 meta-topolin or 2.0 mg l-1 benzyladenine riboside. The longest shoots were formed on media containing benzyladenine riboside at a concentration of 0.5 mg l-1. The length of newly developed shoots was strongly suppressed by high concentrations of different cytokinins, but the suppression effect of a high concentration of meta-topolin on shoot length was less than that of benzyladenine or benzyladenine riboside. In this study meta-topolin and benzyladenine riboside proved to be effective cytokinins to induce adequate shoot proliferation, while benzyladenine was the least active cytokinin

scholarly journals Micropropagation of Bacopa monnieri using Cyanobacterial Liquid Medium

1970 ◽  
Vol 20 (2) ◽  
pp. 225-231 ◽  
Author(s):  
Meenakshi Banerjee ◽  
Priyanka Modi
Keyword(s):  
Plant Tissue ◽  
In Vitro Propagation ◽  
Liquid Culture ◽  
Culture Media ◽  
Shoot Multiplication ◽  
Axillary Node ◽  
Bacopa Monnieri ◽  
Shoot Initiation ◽  
Aulosira Fertilissima

Hot extract of Aulosira fertilissima (cyanobacterium) added in different proportions to MS as a liquid culture media for the in vitro propagation of Bacopa monnieri (L.) Pennell. Maximum numbers of shoots were induced from axillary node in MS media (40 ml) + Aulosira extract (60 ml) and maximum shoot multiplication was observed when Kn (1.0 mg/l) was added in the shoot initiation media (mentioned above). Surprisingly rooting was also found to be best in the same combination of MS + cyanobacterial extract that was used for initiation and multiplication of shoots. On an average within a period of three subcultures (2 - 3 months) the nodal explants generated 400 shoots.  Rooted plantlets were successfully transferred to the field, after acclimation in the net house.   Key words: Baccopa monnieri, Cyanobacterial extract, Regeneration, Acclimation   D.O.I. 10.3329/ptcb.v20i2.6917   Plant Tissue Cult. & Biotech. 20(2): 225-231, 2010 (December)

scholarly journals In vitro Propagation of Thuja occidentalis Through Apical Shoot Culture

1970 ◽  
Vol 16 (1) ◽  
pp. 5-9
Author(s):  
MH Kabir ◽  
PK Roy ◽  
Golam Ahmed
Keyword(s):  
In Vitro Propagation ◽  
Shoot Culture ◽  
Shoot Length ◽  
Shoot Cultures ◽  
In Vitro Plant Regeneration ◽  
Thuja Occidentalis ◽  
Apical Shoot ◽  
Half Strength ◽  
Morphological Variants

In vitro plant regeneration of Thuja occidentalis was obtained in apical shoot cultures from field grown plants. Hormone free MS medium 100% explants produced shoots. The average number of shoots per explant was 6.57 ± 0.45 and the average shoot length of 4.5 ± 0.27 cm were recorded in this medium. Shoots rooted well when they were transferred into half strength MS with 1.0 mg/l IBA. The average number of root per shoot was 3.92 ± 0.28 and the average root length of 3.64 ± 0.38 cm were observed in this medium. No morphological variants were observed during the passage of in vitro culture.Key words: In vitro, Propagation, Thuja occidentalis, Apical shootDOI = 10.3329/ptcb.v16i1.1099Plant Tissue Cult. & Biotech. 16(1): 5-9, 2006 (June)

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