scholarly journals Production of Disease-Free Olive Seedlings with Artificial Intelligence and Biotechnological Methods

2021 ◽  
Vol 2 (3) ◽  
pp. 79-84
Author(s):  
S. Galatali ◽  
M. A. Balci ◽  
O. Akguller ◽  
E. Kaya

It is an inevitable fact that the applications of machine learning and artificial intelligence technologies in agricultural biotechnology approaches, whose applications are increasing rapidly in almost every field, will have an important place in determining the future fate of agriculture. Applications in which artificial intelligence is adapted to biotechnological processes such as breeding, in vitro culture studies, germplasm preservation, disease-free plant production, genetic transformation, and other genetic analyzes are becoming increasingly common. In the current study is highlighted the potential benefits between artificial intelligence and agricultural technologies.  As with many plant species, viral diseases have negative effects on fruit yield, life span, and quality of olive varieties, which are important in economy. Elimination of viruses from the plant with traditional methods is quite laborious, takes a lot of time, and often fails to produce successful results. However, new protocols have been developed to eliminate persistent pathogens. These protocols include techniques such as heat application (thermotherapy), chemical therapy (chemotherapy), tissue culture methods (meristem culture). When these techniques are applied alone or together, it may be possible to obtain anti-virus plants.  Artificial intelligence technology will make it possible to benefit from the method to be used in the most efficient way by revealing which of these biotechnological methods can be used in the most effective and optimal conditions, and the possible advantages and disadvantages as a result of comparing with others.

2020 ◽  
pp. 1-11
Author(s):  
Jianye Zhang

This article analyzes the reform of information services in university physical education based on artificial intelligence technology and conducts in-depth and innovative research on it. In-depth analysis of the relationship between big data and the development and application of information technology such as the Internet, Internet of Things, cloud computing, to clarify the difference and connection between big data, informatization and intelligence. Artificial intelligence will bring opportunities for changes in data collection, management decision-making, governance models, education and teaching, scientific research services, evaluation and evaluation of physical education in our university. At the same time, big data education management in colleges and universities faces many challenges such as the balance of privacy and freedom, data hegemony, data junk, data standards, and data security, and they have many negative effects. In accordance with the requirements of educational modernization, centering on the goal of intelligent and humanized education management, it aims existing issues in college physical education management.


PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e85989 ◽  
Author(s):  
Jorge Gago ◽  
Lourdes Martínez-Núñez ◽  
Mariana Landín ◽  
Jaume Flexas ◽  
Pedro P. Gallego

2019 ◽  
Vol 25 ◽  
pp. 298-303
Author(s):  
S. V. Pykalo ◽  
O. A. Demydov ◽  
T. V. Yurchenko ◽  
N. I. Prokopik ◽  
O. V. Humeniuk

Aim. To investigate the regenerative ability of promising winter common wheat lines in shoot apical meristem culture. Methods. Plant tissue culture methods, statistical evaluation of data. Results. The processes of morphogenesis in culture of apical meristem of 3-days seedlings of lines of winter common wheat were investigated and it was established that the frequency of callusogenesis and shoot regeneration in the studied forms is determined by the genotype of explant. Two types of callus with morphophysiological properties were identified: morphogenic and nonmorphogenic callus. The formation of regenerated plants from wheat calli took place through both gemmorizogenesis and somatic embryogenesis. Conclusions. The line Erytrospermum 60068 was characterized the highest regeneration potential and it can be recommended for further biotechnology of wheat. Obtained technology of vigorous regenerated plant production of winter common wheat lines in shoot apical meristem culture can be used in cell selection and genetic engineering experiments. Keywords: winter common wheat, apical meristem, genotype, callus, shoot regeneration.


Author(s):  
Zeliha Çiftçi ◽  
Mizgin Ay ◽  
Ebru Sakar

Known as the world’s most healthy and natural source of vegetable oil, the history of olives dates back to 10,000 years ago. The homeland of olives, a member of the Oleacea family, is Upper Mesopotamia and Southern Asia, including Southeastern Anatolia and Syria. Olives, BC It started to be cultivated on the eastern shores of the Mediterranean in the year 3000 and is one of the first fruit species cultivated in the Mediterranean region. In this respect, olive has an important place in the economy, nutrition and culture of Mediterranean countries. Currently, in most olive growing countries, olive, leafy stem or cuttings are rooted or by propagating stem shoots from seed or clonal stem. However, the so-called table olives are very difficult or completely impossible to root. The olives, which are very difficult to root, should be supported with biotechnological approaches such as micropropagation method in order to increase the product productivity. So far, many fruit species have been propagated in vitro using tissue culture methods and at the same time, some olive varieties have been successfully propagated by micro-propagation method. It made in tissue culture in the world and Turkey Olives have been compiled resources to work for the researchers in this study.


2021 ◽  
Vol 18 (4) ◽  
pp. 679-691
Author(s):  
Hoang Thanh Tung ◽  
Hoang Dac Khai ◽  
Do Manh Cuong ◽  
Le Van Thuc ◽  
Le The Bien ◽  
...  

Artichoke (Cynara scolymus L.), a high economic value crop, was brought to Vietnam by the French in the late 19th century. The artichoke was mainly planted in Lam Dong, Lao Cai, Vinh Phuc provinces, etc. At present, the disease situation of Artichoke plants and the lack of disease-free seedlings result in insufficient source of Artichoke for producers. Artichoke plants are mainly vegetative propagation and pathogens easily transferred from mother to daughter plants. Therefore, low propagation rate and fungal infection are two main factors hindering the expansion and development of Artichoke cultivation (in Lam Dong). Therefore, studying and evaluating the situation of fungi and viruses as well as establishing the in vitro propagation procedures in order to produce high number of disease-free seedlings are urgent for the current Artichoke shortage. In this study, samples of purple and white Artichoke varieties, which suspected fungal manifestations, were collected to assessment of fungi and viruses in artichoke. In addition, in vitro propagation by cultivating apical meristem was applied to produce disease-free seedlings. The recorded results showed that, 19 strains of mold were identified on purple and white Artichoke belonged to nine genera including Mucor (M. sp., M. circinelloides, M. fragilis, M. irregularis, and M. racemosus), Alternaria (Alternaria sp., A. alterinata, A. gaisen, A. tenuissima, and A. tillandsiae), Fusarium (F. acuminatum and F. solani), Cylindrobasidium (Cylindrobasidium sp1 and Cylindrobasidium sp2), Actinomucor elegans, Curvalaria clavata, Plectosphaerella oligotrophica, Phoma herbarum, Rhizomucor variabillis; meanwhile, the Tomato mosaic virus (ToMV) was isolated only purple Artichoke. Shoot explants obtained from apical meristem culture were completely disease-free and used for micropropagation at the next stage.


1970 ◽  
Vol 16 (1) ◽  
pp. 31-36 ◽  
Author(s):  
AKMN Huda ◽  
B Sikdar ◽  
B Sikdar

The growth of meristem was observed on semisolid MS medium supplemented with 0.05 mg/l Kn + 0.1 mg/l GA3. After three weeks, meristems were transferred to MS supplemented with BA, Kn, IBA, NAA and IAA singly or in combination for shoot elongation and root initiation. Among different treatments for shoot initiation with elongation were obtained in MS supplemented with 1.0 mg/l BA + 0.1 mg/l IBA + 0.3 mg/l GA3. On the other hand good rooting was observed when 0.5 mg/l IBA and 0.1 mg/l NAA were used to fortify MS semisolid medium. Ten weeks old in vitro plantlets were successfully planted in soil through gradual acclimation.Key words: Apical meristem, Bitter gourd, In vitro, Plant production DOI = 10.3329/ptcb.v16i1.1103Plant Tissue Cult. & Biotech. 16(1): 31-36, 2006 (June)


Horticulturae ◽  
2021 ◽  
Vol 7 (5) ◽  
pp. 111
Author(s):  
Andrea Copetta ◽  
Miriam Bazzicalupo ◽  
Arianna Cassetti ◽  
Ilaria Marchioni ◽  
Carlo Mascarello ◽  
...  

Mertensia maritima is a commercially interesting herb with edible leaves and flowers, characterized by oyster flavor and taste. Plant propagation and traditional cultivation are challenging for this species. Therefore, the main purpose of the present study was to establish successful protocols aimed at ensuring oyster plant shoot propagation, rooting and in vivo acclimatization. Both micropropagation and rooting were tested, comparing the traditional in vitro solid substrate in jar vs. the liquid culture in a temporary immersion system (TIS) bioreactor (Plantform™). A Murashige and Skoog (MS) medium added with 4-µM thidiazuron (TDZ) and 1-µM α-naphthaleneacetic acid (NAA) was employed for micropropagation, while a half-strength MS medium supplemented with 4-µM indole−3-butyric acid (IBA) was used for rooting. Different acclimatization conditions in the greenhouse or in growth chamber were tested. Morphometric and microscopical analyses were performed on the oyster plant leaves at the propagation, rooting and acclimatization stages both in a jar and in a TIS. Micropropagation in a TIS allowed to obtain large shoots, while a great number of shoots was observed in the jar. M. maritima shoots rooted in TIS produced more developed roots, leaves with more developed waxy glands and well-formed stomata; moreover, the plants coming from the TIS showed the best acclimatization performances.


2018 ◽  
Vol 5 (2) ◽  
pp. 13-17
Author(s):  
O. Klyachenko ◽  
O. Nekrut

Aim. To obtain planting material of Miscanthus × giganteus in a large amount in order to study the peculiari- ties of morphogenesis of isolated meristem culture. Methods. The explants were sterilized using 70 % etha- nol and 0.1 % mercury bichloride, after that, the methods of plant cultivation and reproduction in vitro and statistical processing of the obtained data was performed. Results. The study presents the results of obtaining the regenerate plants from dormant buds of 3-year-old rhizomes of Miscanthus × giganteus. The Murashige and Skoog medium supplemented with 6-benzylaminopurine (BAP) (0.75 mg/L) and kinetin (1.2 mg/L) was found to be the best for regeneration of microsprouts, providing the maximum value of the regeneration index (95 %) and contributing to the development of the main sprouts and tillering. The obtained sprouts were rooted in Murashige and Skoog medium supplemented with half-dose of macro- and micronutrients without growth regulators. In doing so, rooting index made up 95.0–97.0 %. The best substrate for the adaptation of miscan- thus plants in vivo was found to be a mixture of peat, sand, and perlite in a ratio of 2 : 1 : 1, respectively. In this substrate, plant establishment reached 91 %. Conclusions. The peculiarities of morphogenesis in Miscan- thus × giganteus isolated meristem were studied. At the same time, the index of sprouts regeneration varied from 90.0 to 100.0 %. In addition, the development of the main sprout and multiple tillering occurred in 85.0– 100.0 % explant. The highest survivability of micro-plants (91–95 %) was provided in the peat-sand-perlite substrate in a ratio of 2 : 1 : 1.


2006 ◽  
Vol 5 (4) ◽  
pp. 443-448 ◽  
Author(s):  
Suriyan Cha-um ◽  
Nguyen Thi-Thanh Hie . ◽  
Chalermpol Kirdmanee .

Sign in / Sign up

Export Citation Format

Share Document