POSSIBLE ENHANCEMENT OF PHOSPHATIDYL INOSITOL (PI) DEPENDS ON ITS METABOLIC PATHWAY COMPONENTS AS SECOND MESSENGERS: EVIDENCE FROM THE GROWTH AND PI LEVELS OF NORMAL, PI GROWN, AND UV EXPOSED SACCHAROMYCES CEREVISIAE.

2018 ◽  
Vol 7 (1) ◽  
pp. 26-31 ◽  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Pathway ◽  
Second Messengers ◽  
Phosphatidyl Inositol

Effects of metabolic pathway gene copy numbers on the biosynthesis of (2S)-naringenin in Saccharomyces cerevisiae

2021 ◽  
Vol 325 ◽  
pp. 119-127
Author(s):  
Hongbiao Li ◽  
Song Gao ◽  
Siqi Zhang ◽  
Weizhu Zeng ◽  
Jingwen Zhou
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Pathway ◽  
Gene Copy ◽  
Copy Numbers ◽  
Pathway Gene ◽  
Gene Copy Numbers

Genetically Engineered Saccharomyces cerevisiae Strain that Can Ultilize Both Xylose and Glucose for Fermentation

2013 ◽  
Vol 448-453 ◽  
pp. 1637-1643
Author(s):  
Jing Ping Ge ◽  
Lu Yan Zhang ◽  
Wen Xiang Ping ◽  
Meng Yun Zhang ◽  
Yan Shen ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Pathway ◽  
Ethanol Yield ◽  
Transformation Method ◽  
Fuel Ethanol ◽  
Genetically Engineered ◽  
Lithium Acetate ◽  
Expression Plasmid ◽  
Primary Problem

The primary problem in producing fuel ethanol through microorganism fermentation with lignocellulose is the strain. We constructed a URA3-directed low copy integration-expression plasmid pZMYBX1 and rDNA-directed high copy integration-expression plasmid pZMYX2. Using the lithium acetate transformation method, we co-transformed the linearized plasmid pZMYBX1 (StuI) and pZMYX2 (HpaI) into theS. cerevisiaecells. Ultimately, we obtain three recombinants: HDY-ZMYWBG1, HDY-ZMYWBG2 and HDY-ZMYWBG3. The ethanol yield for HDY-ZMYWBG1 and HDY-ZMYWBG3 are 0.368 g/g and 0.365 g/g, respectively, which are higher than the 0.330 g/g yield for W5. This findings show that the xylose metabolic pathway could be introduced into theS. cerevisiaeto produce an alternative strain for the production of biological ethanol from lignocellulose substrate.

Solubilization of microsomal-associated phosphatidylserine synthase and phosphatidylinositol synthase from Saccharomyces cerevisiae

1981 ◽  
Vol 27 (11) ◽  
pp. 1140-1149 ◽  
Author(s):  
George M. Carman ◽  
Jonathan Matas
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Specific Activity ◽  
Phosphatidyl Inositol ◽  
Maximum Activity ◽  
Enzymatic Activities ◽  
Ph Optimum ◽  
Phosphatidylserine Synthase ◽  
Phosphatidylinositol Synthase ◽  
Microsome Fraction ◽  
Triton X

Membrane-associated cytidine 5′-diphospho-1,2-diacyl-sn-glycerol (CDP-diacylglycerol):L-serine O-phosphatidyltransferase (phosphatidylserine synthase, EC 2.7.8.8.) and CDP-diacylglycerol: myo-inositol phosphatidyltransferase (phosphatidyl-inositol synthase, EC 2.7.8.11) were solubilized from the microsomal fraction of Saccharomyces cerevisiae. A variety of detergents were examined for their ability to release phosphatidylserine synthase and phosphatidylinositol synthase activities from the microsome fraction. Both enzymes were solubilized from the microsome fraction with Renex 690 in yields over 80% with increases in specific activity of 1.6-fold. Both solubilized enzymatic activities were dependent on manganese ions and Triton X-100 for maximum activity. The pH optimum for each reaction was 8.0. The apparent Km values for CDP-diacylglycerol and serine for the phosphatidylserine synthase reaction were 0.1 and 0.25 mM, respectively. The apparent Km values for CDP-diacylglycerol and inositol for the phosphatidylinositol synthase reaction were 70 μM and 0.1 mM, respectively. Thiore-active agents inhibited both enzymatic activities. Both solubilized enzymatic activities were thermally inactivated at temperatures above 30 °C.

Evolution of a Saccharomyces cerevisiae metabolic pathway in Escherichia coli

2007 ◽  
Vol 9 (2) ◽  
pp. 152-159 ◽  
Author(s):  
Isabelle Meynial Salles ◽  
Nynne Forchhammer ◽  
Christian Croux ◽  
Laurence Girbal ◽  
Philippe Soucaille
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Metabolic Pathway

Signalling functions for sphingolipid long-chain bases in Saccharomyces cerevisiae

2005 ◽  
Vol 33 (5) ◽  
pp. 1170-1173 ◽  
Author(s):  
K. Liu ◽  
X. Zhang ◽  
C. Sumanasekera ◽  
R.L. Lester ◽  
R.C. Dickson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Kinase ◽  
Second Messengers ◽  
Dependent Protein Kinase ◽  
Yeast Saccharomyces Cerevisiae ◽  
Signalling Molecules ◽  
Cellular Processes ◽  
Wide Range ◽  
Dependent Protein ◽  
Long Chain

Over the past several years, studies of sphingolipid functions in the baker's yeast Saccharomyces cerevisiae have revealed that the sphingoid LCBs (long-chain bases), dihydrosphingosine and PHS (phytosphingosine), are important signalling molecules or second messengers under heat stress and during non-stressed conditions. LCBs are now recognized as regulators of AGC-type protein kinase (where AGC stands for protein kinases A, G and C) Pkh1 and Pkh2, which are homologues of mammalian phosphoinositide-dependent protein kinase 1. LCBs were previously shown to activate Pkh1 and Pkh2, which then activate the downstream protein kinase Pkc1. We have recently demonstrated that PHS stimulates Pkh1 to activate additional downstream kinases including Ypk1, Ypk2 and Sch9. We have also found that PHS acts downstream of Pkh1 and partially activates Ypk1, Ypk2 and Sch9. These kinases control a wide range of cellular processes including growth, cell wall integrity, stress resistance, endocytosis and aging. As we learn more about the cellular processes controlled by Ypk1, Ypk2 and Sch9, we will have a far greater appreciation of LCBs as second messengers.

scholarly journals Ancient balancing selection maintains incompatible versions of a conserved metabolic pathway in yeast

2019 ◽  
Author(s):  
James Boocock ◽  
Meru J Sadhu ◽  
Joshua S Bloom ◽  
Leonid Kruglyak
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Pathway ◽  
Genetic Interaction ◽  
Balancing Selection ◽  
Sensu Stricto ◽  
The Other ◽  
Reference Allele ◽  
Metabolic Genes ◽  
Species Cluster ◽  
Metabolic Strategies

AbstractDifferences in nutrient availability have led to the evolution of diverse metabolic strategies across species, but within species these strategies are expected to be similar. Here, we discovered that the galactose metabolic pathway in the yeast Saccharomyces cerevisiae exists in two functionally distinct, incompatible states maintained by ancient balancing selection. We identified a genetic interaction for growth in galactose among the metabolic genes GAL2, GAL1/10/7, and PGM1. We engineered strains with all allelic combinations at these loci and showed that the reference allele of PGM1 is incompatible with the alternative alleles of the other genes. We observed a strong signature of ancient balancing selection at all three loci and found that the alternative alleles diverged from the reference alleles before the birth of the Saccharomyces sensu stricto species cluster 10-20 million years ago. Strains with the alternative alleles are found primarily in galactose-rich dairy environments, and they grow faster in galactose, but slower in glucose, revealing a tradeoff on which balancing selection may have acted.

Metabolic Pathway Deficiencies are Compensated by Higher Energy Dissipation to Maintain Microbiological Growth rate in Saccharomyces cerevisiae

2020 ◽  
Vol 159 ◽  
pp. S64
Author(s):  
Rafael Bento ◽  
Cátia Marques ◽  
Carlos Bernardes ◽  
Gonçalo Justino ◽  
Manuel Piedade ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Energy Dissipation ◽  
Metabolic Pathway

Increase ethyl acetate production in Saccharomyces cerevisiae by genetic engineering of ethyl acetate metabolic pathway

2019 ◽  
Vol 46 (6) ◽  
pp. 801-808 ◽  
Author(s):  
Jian Dong ◽  
Pengfei Wang ◽  
Xiaomeng Fu ◽  
Shengsheng Dong ◽  
Xiao Li ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Engineering ◽  
Ethyl Acetate ◽  
Metabolic Pathway ◽  
Acetate Production
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