scholarly journals Simultaneous HPLC Determination of Lidocaine Hydrochloride and Hexachlorophene in a Suppository Product

2020 ◽  
Vol 3 (1) ◽  
pp. 27-36
Author(s):  
Cece Furwanti ◽  
Kusuma Hendrajaya ◽  
Gunawan Indrayanto
Keyword(s):  
Simultaneous Determination ◽  
Method Validation ◽  
Phosphate Buffer ◽  
Mobile Phase ◽  
Hplc Method ◽  
Lidocaine Hydrochloride ◽  
Analysis Method ◽  
New Methods ◽  
Pharmaceutical Industries

Antihemoroid® suppository has been produced commercially by PT. Kimia Farma, Indonesia. For QC purposes, a separated densitometric method for analysis of its active ingredients, lidocaine hydrochloride and hexachlorophene, was applied. The objective of this study was obtaining more efficient analysis method of LH and HC, therefore an HPLC procedure has been developed for the determination of both compounds simultaneously. AYMC-Triart C18 column was used with a gradient mobile phase consisting of acetonitrile and phosphate buffer 0.05 M (pH 6.0). Quantitative evaluation was performed at 220 nm. Method validation was performed according to the new methods of USP 41. Result showed that the HPLC method was simple, accurate, precise, and robust. The HPLC method can be applied in simultaneous determination of LH and HC in suppositories as a QC tool in the pharmaceutical industries.

scholarly journals Method validation for simultaneous quantification of whey proteins in dietary supplements by HPLC

2019 ◽  
Vol 2 (1) ◽  
pp. 32-38
Author(s):  
Hong Ngoc Nguyen Thi ◽  
Thanh Hoa Mac Thi ◽  
Thanh An Vu Thi ◽  
Thanh Ha Pham Thi ◽  
Khanh Cao Cong ◽  
...  
Keyword(s):  
Dietary Supplements ◽  
Simultaneous Determination ◽  
Method Validation ◽  
Mobile Phase ◽  
Whey Proteins ◽  
Hplc Method ◽  
Simultaneous Quantification ◽  
Precision And Accuracy ◽  
Beta Lactoglobulin

A HPLC method has been validated for simultaneous determination of Alpha-lactalbumin (α- LA) and Beta- lactoglobulin (β-LG) contents in dietary supplements by HPLC-PDA. The method was carried out in C18 column with a gradient of 0.1% TFA/ water – 0.1% TFA/ acetonitrile as mobile phase. The proteins were detected at 215 nm wavelength within 50 minutes. The method was validated in specificity, linearity, precision and accuracy; and then was applied to analyze several random dietary supplements.

scholarly journals Simultaneous Determination of Preservatives (Methyl Paraben and Propyl Paraben) in Sucralfate Suspension Using High Performance Liquid Chromatography

2011 ◽  
Vol 8 (1) ◽  
pp. 340-346 ◽  
Author(s):  
Rajesh M. Kashid ◽  
Santosh G. Singh ◽  
Shrawan Singh
Keyword(s):  
High Performance Liquid Chromatography ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Methyl Paraben ◽  
Propyl Paraben

A reversed phase HPLC method that allows the separation and simultaneous determination of the preservatives methyl paraben (M.P.) and propyl paraben (P.P.) is described. The separations were effected by using an initial mobile phase of water: acetonitrile (50:50) on Inertsil C18 to elute P.P. and M.P. The detector wavelength was set at 205 nm. Under these conditions, separation of the two components was achieved in less than 10 min. Analytical characteristics of the separation such as precision, specificity, linear range and reproducibility were evaluated. The developed method was applied for the determination of preservative M.P. and P.P. at concentration of 0.01 mg/mL and 0.1 mg/mL respectively. The method was successfully used for determining both compounds in sucralfate suspension.

scholarly journals Stability-Indicating Validated HPLC Method for Simultaneous Determination of Oral Antidiabetic Drugs from Thiazolidinedione and Sulfonylurea Groups in Combined Dosage Forms

2010 ◽  
Vol 93 (4) ◽  
pp. 1086-1092 ◽  
Author(s):  
Anna Gumieniczek ◽  
Anna Berecka ◽  
ukasz Komsta
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Uv Light ◽  
Degradation Products ◽  
Dosage Forms ◽  
Hplc Method ◽  
Base Temperature ◽  
Stability Indicating ◽  
Acid And Base

Abstract For type 2 diabetes treatment, combinations of drugs from the thiazolidinedione and sulfonylurea groups are now available in the same tablet or capsule. Therefore, a stability-indicating and validated HPLC method was developed for simultaneous determination of pioglitazone, rosiglitazone, and glipizide in combined dosage forms. The examined drugs were subjected to different conditions such as acid and base, temperature, and UV light, and degradation of pioglitazone and glipizide was observed under thermal and acidic stress. However, selectivity of the presented method for pioglitazone, rosiglitazone, and glipizide assay against their degradation products was confirmed. It was also demonstrated to be robust, resisting small deliberate changes in pH of the buffer, flow rate, and percentage of acetonitrile in the mobile phase. The presented method utilizes a LiChrospher RP18 column (125 4.0 mm), acetonitrile in phosphate buffer at pH 4.3 (40 + 60, v/v) as the mobile phase, and UV detection at 225 nm for pioglitazone/glipizide or 245 nm for rosiglitazone/glipizide. The method was validated with respect to linearity, precision, and accuracy. Finally, the elaborated procedure was applied for the QC of pioglitazone/glipizide and rosiglitazone/glipizide mixtures.

scholarly journals Simultaneous HPLC Determination of Chlordiazepoxide and Mebeverine HCl in the Presence of Their Degradation Products and Impurities

2015 ◽  
Vol 2015 ◽  
pp. 1-9
Author(s):  
Rania N. El-Shaheny ◽  
Fathalla F. Belal
Keyword(s):  
Simultaneous Determination ◽  
Degradation Product ◽  
Mobile Phase ◽  
Degradation Products ◽  
Potassium Dihydrogen Phosphate ◽  
Hplc Method ◽  
Dihydrogen Phosphate ◽  
Ir Studies ◽  
Validation Procedure

A simple, rapid, and sensitive RP-HPLC method was developed and validated for the simultaneous determination of chlordiazepoxide (CDO) and mebeverine HCl (MBV) in the presence of CDO impurity (2-amino-5-chlorobenzophenone, ACB) and MBV degradation product (veratric acid, VER). Separation was achieved within 9 min on a BDS Hypersil phenyl column (4.5 mm × 250 mm, 5 µm particle size) using a mobile phase consisting of acetonitrile: 0.1 M potassium dihydrogen phosphate: triethylamine (35 : 65 : 0.2, v/v/v) in an isocratic mode at a flow rate of 1 mL/min. The pH of the mobile phase was adjusted to 4.5 with orthophosphoric acid and UV detection was set at 260 nm. A complete validation procedure was conducted. The proposed method exhibited excellent linearity over the concentration ranges of 1.0–100.0, 10.0–200.0, 2.0–40.0, and 2.0–40.0 µg/mL for CDO, MBV, VER, and ACB, respectively. The proposed method was applied for the simultaneous determination of CDO and MBV in their coformulated tablets with mean percentage recoveries of 99.75 ± 0.62 and 98.61 ± 0.38, respectively. The results of the proposed method were favorably compared with those of a comparison HPLC method using Studentt-test and the variance ratioF-test. The chemical structure of MBV degradation product was ascertained by mass spectrometry and IR studies.

scholarly journals Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft Drinks

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Bürge Aşçı ◽  
Şule Dinç Zor ◽  
Özlem Aksu Dönmez
Keyword(s):  
Simultaneous Determination ◽  
Flow Rate ◽  
Mobile Phase ◽  
High Performance ◽  
Food Additives ◽  
Hplc Method ◽  
Soft Drinks ◽  
Phase Ratio ◽  
Potassium Sorbate

Box-Behnken design was applied to optimize high performance liquid chromatography (HPLC) conditions for the simultaneous determination of potassium sorbate, sodium benzoate, carmoisine, allura red, ponceau 4R, and caffeine in commercial soft drinks. The experimental variables chosen were pH (6.0–7.0), flow rate (1.0–1.4 mL/min), and mobile phase ratio (85–95% acetate buffer). Resolution values of all peak pairs were used as a response. Stationary phase was Inertsil OctaDecylSilane- (ODS-) 3V reverse phase column (250 × 4.6 mm, 5 μm) dimensions. The detection was performed at 230 nm. Optimal values were found 6.0 pH, 1.0 mL/min flow rate, and 95% mobile phase ratio for the method which was validated by calculating the linearity (r2>0.9962), accuracy (recoveries ≥ 95.75%), precision (intraday variation ≤ 1.923%, interday variation ≤ 1.950%), limits of detection (LODs), and limits of quantification (LOQs) parameters. LODs and LOQs for analytes were in the range of 0.10–0.19 μg/mL and 0.33–0.63 μg/mL, respectively. The proposed method was applied successfully for the simultaneous determination of the mixtures of five food additives and caffeine in soft drinks.

scholarly journals Determination of Mefenamic Acid in Pharmaceutical Drugs and Wastewater by RP-HPLC

2017 ◽  
Vol 2 (2) ◽  
pp. 85-88
Author(s):  
Mohammad Anas Alfeen
Keyword(s):  
Mefenamic Acid ◽  
Chromatographic Analysis ◽  
Mobile Phase ◽  
Pharmaceutical Preparations ◽  
Hplc Method ◽  
Treated Wastewater ◽  
Pharmaceutical Drugs ◽  
Analysis Method ◽  
Rp Hplc

This study presents a chromatographic analysis method for the determination of mefenamic acid in pharmaceutical preparations and treated wastewater from the pharmaceutical industry in East Asia and specifically (Syria). An isocratic RP-HPLC method has been developed for determination of Mefenamic acid on a Grace, alltima C18 column (250 x 4.6 mm, 5.0 μm) using a mobile phase consisting of (1%) Triethylamine aqueous buffer adjust pH = 2 by H3PO4 (85%): Methanol: Acetonitrile); (35: 20: 45 v\v\v %) at a flow rate of 2 mL/min. Detection was carried out at 220 nm. Retention time of Mefenamic acid was 7.85 ( ± 0.36) mins. The method was validated with respect to specificity, linearity, accuracy, precision, ruggedness, and robustness.  The proposed method is simple, precise, sensitive, and reproducible and is applicable for quantification of Mefenamic acid in Tablets formulations and Wastewater developed and formulated in our laboratory.

scholarly journals HPLC Method for Determination of Aspirin, Rosuvastatin, Ezetimibe and Clopidogrel in Combination Drug Products

2019 ◽  
Vol 31 (10) ◽  
pp. 2275-2283 ◽  
Author(s):  
Suresh Kumar Palacharla ◽  
G.V. Krishna Mohan
Keyword(s):  
Method Validation ◽  
Flow Rate ◽  
Mobile Phase ◽  
Drug Product ◽  
Hplc Method ◽  
Combination Drug ◽  
Injection Volume ◽  
Drug Products ◽  
Product Manufacturing

A single HPLC method for the determination of four active ingredients viz. aspirin, rosuvastatin, ezetimibe and clopidogrel in less run time is developed. HPLC method was developed and validated. X-terra C18 100 × 4.6 mm, 3.5 μ HPLC column, KH2PO4 buffer (mobile phase A) and acetonitrile (mobile phase B) were used. 20 μ injection volume, 1.0 mL/min flow rate, 230 nm and ambient column oven temperature were applied for separation. Gradient program: at 0 min mobile phase-B 13 %, at 4 min 13 %, at 8 min 44 %, 14 min 57 %, 17 min 57 %, 20 min 13 % and 25 min 13 %. Method validation was performed as per ICH guidance with precision, linearity, specificity, accuracy, ruggedness and robustness. Validation results were satisfactory and this method can be applied for regular drug product manufacturing

scholarly journals Simultaneous determination of ethinyl estradiol and drospirenone in oral contraceptive by high performance liquid chromatography

2013 ◽  
Vol 49 (3) ◽  
pp. 521-528 ◽  
Author(s):  
Viviane Benevenuti Silva ◽  
Angel Arturo Gaona Galdos ◽  
Cintia Maria Alves Mothe ◽  
Michele Bacchi Pallastrelli ◽  
Maria Segunda Aurora Prado ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Ethinyl Estradiol ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Elution Time ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

A simple, rapid, economical and reliable high performance liquid chromatographic method has been developed and successfully applied in simultaneous determination of ethinyl estradiol and drospirenone in coated tablets. The HPLC method was performed on a LiChroCART® 100RP column (125x4 mm i.d., 5 µm) with acetonitrile:water 50:50 (v/v) as mobile phase, pumped at a flow rate of 1.0 mL.min-1. The fluorescence detection for ethinyl estradiol was made at λex= 280 nm and λem= 310 nm and a UV detection for drospirenone was made at 200 nm. The elution time for ethinyl estradiol and drospirenone were 4.0 and 5.7 min, respectively. The method was validated in accordance to USP 34 guidelines. The proposed HPLC method presented advantages over reported methods and is suitable for quality control assays of ethinyl estradiol and drospirenone in coated tablets.

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