scholarly journals HEMATOLOGICAL AND BIOCHEMICAL INDICATORS OF AUSTRALIAN RED-CLAW CRAYFISH HEMOLYMPH

2021 ◽  
Vol 2021 (2) ◽  
pp. 134-143
Author(s):  
Lina Yurievna Lagutkina ◽  
Elena Evgrafova ◽  
Evgeniia Germanovna Kuzmina ◽  
Alexey Mikhaylovich Mazlov
Keyword(s):  
Physiological State ◽  
Cholesterol Content ◽  
Freshwater Crayfish ◽  
Astacus Leptodactylus ◽  
Biochemical Indicators ◽  
Cherax Quadricarinatus ◽  
Freshwater Aquaculture ◽  
Red Claw Crayfish ◽  
High Level ◽  
Australian Freshwater

The article describes Australian red claw crayfish, Cherax quadricarinatus, which is currently one of the most promising cultivation targets. However, to increase production volumes, it is necessary to improve cultivation technologies, which should be based on the data of physiological and biochemical parameters. An objective method for monitoring the physiological state of Australian red claw crayfish in the artificial conditions is determining its hematological and biochemical indicators that complement the general characteristics of hemolymph and later define its physiological norm. When giving the objects the compound feed, which the authors produced by their own recipe, there was recorded a high level of total hemolymph protein of 40.8 ± 4.5 g/l. According to the results of biochemical research, the cholesterol content in the hemolymph of Australian crayfish was 3.2 ± 0.6 mmol/l, and the concentration of lipoproteins was 0.8 ± 0.2 g/l (levels within the reference values). When considering the hemogram of hemolymph there were found the dominant types and linear dimensions of native formed elements. The diameter of agranulocytes in Australian freshwater crayfish varied from 85 to 90 μm, semi-granulocytes - from 90 to 95 μm, granulocytes - from 65 to 78 μm, transparent cells differed by greater variability in size, which ranged from 85 to 120 μm. The part of granulocytes in relation to other shaped elements exceeded 50%, for transparent cells it turned out to be above 20%, for semi-granulocytes - about 15% and for agranulocytes - about 5%, which means that the latter form the rarest group. The established ratios are specific for Cherax quadricarinatus, its hemocytic characteristics greatly differs from Astacus leptodactylus, which is the main object of comparative research. The results of comparative analysis of the cellular composition of the hemolymph of the Australian freshwater crayfish Cherax quadricarinatus (Von Martens, 1868) demonstrate the taxonomic specificity of this representative of freshwater aquaculture

scholarly journals Detection of Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV, Decapod Penstylhamaparvovirus 1) in Commodity Red Claw Crayfish (Cherax quadricarinatus) Imported into South Korea

2021 ◽  
Vol 9 (8) ◽  
pp. 856
Author(s):  
Chorong Lee ◽  
Seong-Kyoon Choi ◽  
Hye Jin Jeon ◽  
Seung Ho Lee ◽  
Young Kyoon Kim ◽  
...  
Keyword(s):  
South Korea ◽  
Procambarus Clarkii ◽  
Penaeid Shrimp ◽  
Freshwater Crayfish ◽  
Asian Countries ◽  
Necrosis Virus ◽  
Tissue Samples ◽  
Cherax Quadricarinatus ◽  
Pathogenic Viruses ◽  
Red Claw Crayfish

Freshwater crayfish, which are cultivated in aquaculture, are economically important for food and ornamental purposes. However, relatively few studies have focused on potentially pathogenic viruses in crayfish compared to in penaeid shrimp. Commodity red claw crayfish (Cherax quadricarinatus; 400 crayfish in 10 batches) and red swamp crayfish (Procambarus clarkii; 40 crayfish in 2 batches) imported into South Korea from Indonesia and China were screened by PCR to detect infectious hypodermal and hematopoietic necrosis virus (IHHNV or Decapod penstylhamaparvovirus 1). IHHNV was detected in tissue samples pooled from nine out of ten batches of red claw crayfish imported from Indonesia. Phylogenetic analysis of PCR amplicons from representative pools clustered the IHHNV strain with infectious-type II sequences commonly detected in Southeast Asian countries rather than with type III strains detected previously in whiteleg shrimp (Penaeus vannamei) cultured in South Korea. IHHNV DNA was detected most frequently in the muscle (eight batches, 66.7% samples), followed by in the hepatopancreas (five batches, 41.7% samples) and gills tissue (three batches, 25.0% samples). These data suggest that red claw crayfish could be a potential carrier of the virus and that quarantine procedures must be strengthened in South Korea to avoid importing infectious types of IHHNV in commodity crustaceans such as red claw crayfish.

scholarly journals Bioindicators of homeostasis’ constants of growing conditions of warm-water aquaculture objects in the context of obtaining marketable products

2021 ◽  
Vol 937 (3) ◽  
pp. 032032
Author(s):  
Aliya Akhmedzhanova ◽  
Elena Evgrafova ◽  
Yulia Fedorovykh ◽  
Lina Lagutkina ◽  
Sergey Ponomarev ◽  
...  
Keyword(s):  
Warm Water ◽  
Blood Protein ◽  
Total Serum Protein ◽  
Total Serum ◽  
Freshwater Crayfish ◽  
Missing Information ◽  
Total Blood ◽  
Growing Conditions ◽  
High Level ◽  
Australian Freshwater

Abstract To create recommendations regarding the technological process of growing objects of warm-water aquaculture, the health of individuals in the created conditions of maintain is a necessary study. Taking into account the similarity of the necessary conditions for the organization of growing warm-water aquaculture objects, it is important to monitor the indicators that are bioindicators of homeostasis constants with a projection on the conditions of maintain, for subsequent adjustment of satisfactory conditions of maintain. First of all, these are hematological and biochemical bioindicators that determine the general characteristics of blood in the case of fish objects of sterlet and hemolymph in the case of crustacean objects of Australian freshwater crayfish and the concentration index of total serum protein that determines the “physiological norm”. When growing sterlet, a high level of total blood protein was established at 40.14±1.73 g/l and the hemolymph of the Australian freshwater crayfish was determined to have a protein concentration of 40.8±4.5 g/l. The presented analysis of bioindicators complements the missing information for monitoring the data of representatives of warm-water aquaculture, which will be valuable for specialists engaged in breeding these objects.

INFLUENCE OF HIGH-PROTEIN FEED ADDITIVE ON THE PRODUCTIVITY OF THE MEAT BULL-CALVES

2019 ◽  
Vol 2019 (3) ◽  
pp. 6-11
Author(s):  
Батырхан Абилов ◽  
Batyrkhan Abilov ◽  
Лариса Пашкова ◽  
Larisa Pashkova
Keyword(s):  
Hematological Parameters ◽  
Physiological State ◽  
Average Daily Gain ◽  
Live Weight ◽  
Feed Additive ◽  
North Caucasus ◽  
Agricultural Enterprise ◽  
Bull Calves ◽  
Economic Indices ◽  
High Level

The article presents the data obtained as a result of carrying by employees of the Research Institute of sheep and goat breeding, a branch of the FSBSI "North Caucasus FARC", the scientific and economic experience to study the efficiency of a new fodder additive "Organic" on the productive and physiological indices of bull-calves during the completion of growing in the conditions of the farm "Pata" agricultural enterprise in the Karachay-Cherkessia Republic. The statement of the experience and all researches were carried out according to generally accepted methods. In the course of the experiment, the following indices were studied: the chemical compound of forages, the dynamics of a live weight gain in the experimental stock, hematological parameters, control slaughter data, economic indices. Completed researches have shown positive result from the use of the fodder additive "Organic" in bull-calves diets: an increase in the average daily gain of live weight by 25%, a slaughter yield by 1% and a high level of profitability – 27%. Thus, the additional introduction of "Organic" in a diet of young horned cattle in the 2nd experimental group promoted more intensive metabolism, stabilization of a physiological state, increase in productivity and, as a consequence, profitability of beef production. Researches in this direction reveal the latent fodder reserves promoting increase in the productivity of agricultural animals.

Phylogeny of the Australian freshwater crayfish Cherax destructor-complex (Decapoda : Parastacidae) inferred from four mitochondrial gene regions

2005 ◽  
Vol 19 (3) ◽  
pp. 209 ◽  
Author(s):  
Thuy T. T. Nguyen ◽  
Christopher M. Austin
Keyword(s):  
16S Rrna ◽  
Cytochrome Oxidase ◽  
Mitochondrial Gene ◽  
Large Subunit ◽  
Strong Support ◽  
Interspecific Variation ◽  
Rrna Gene ◽  
Freshwater Crayfish ◽  
Cherax Destructor ◽  
Australian Freshwater

The phylogenetic relationships among 32 individuals of Australian freshwater crayfish belonging to the Cherax destructor-complex were investigated using a dataset comprising sequences from four mitochondrial gene regions: the large subunit rRNA (16S rRNA), cytochrome oxidase I (COI), adenosine triphosphatase 6 (ATPase 6), and cytochrome oxidase III (COIII). A total of 1602 bp was obtained, and a combined analysis of the data produced a tree with strong support (bootstrap values 94–100%) for three divergent lineages, verifying the phylogenetic hypotheses of relationships within the C. destructor species-complex suggested in previous studies. Overall, sequences from the 16S rRNA gene showed the least variation compared to those generated from protein coding genes, which presented considerably greater levels of divergence. The level of divergence within C. destructor was found to be greater than that observed in other species of freshwater crayfish, but interspecific variation among species examined in the present study was similar to that reported previously.

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