Potensi Bakteri Pereduksi Sulfat dan Jenis Bahan Organik dalam Pengolahan Air Asam Tambang menggunakan System Constructed Wetland Tanaman akar Wangi (Vetiveria zizanioides L)

SoilREns ◽  
2021 ◽  
Vol 18 (2) ◽  
Author(s):  
Pujawati Suryatmana ◽  
Apong Sandrawati ◽  
Ikrar Nusantara Putra ◽  
Nadia N. Kamaluddin
Keyword(s):  
Organic Matter ◽  
Constructed Wetland ◽  
Mine Drainage ◽  
Block Design ◽  
Waste Product ◽  
Standard Condition ◽  
Polluted Water ◽  
Vetiveria Zizanioides ◽  
Water Ph ◽  
Reducing Bacteria

Acid mine drainage (AMD) is a waste product from coal mining process. Acidic waste will impose a serious environmental issue such as organism annihilation and destruction of environmental ecosystem. Proper AMD management is required in order to restore polluted water to the proper standard condition. The Constructed Wetland system with Akar Wangi (Vetiveria zizanioides L.) and various organic growth medium compositions and sulphate-reducing bacteria and the composition of the media for several types of organic matter with sulfate-reducing bacteria (SRB) inoculation as stimulant was deemed a promising solution. This study was designed in randomized block design (RBD) consisted of nine treatments with three replicates. The treatments were: (A) = without organic matter and without SRB inoculation (control), (B) = 100% compost, (C) = 100% sawdust, (D) = 50% compost + 50% sawdust, (E ) = no organic matter + SRB inoculation (105 MPN ml-1), (F) = 100% compost + SRB inoculation, (G) = 100% sawdust + SRB inoculation, H = 50% compost + 50% sawdust + SRB inoculation, I = 75% compost + 25% sawdust + SRB inoculation. Results showed that 100% compost and SRB application increased polluted water pH. Application of 100% sawdust with or without SRB inoculation gave the highest reduction in sulfate content. SRB also has the potential to increase the height of Akar Wangi plant.

scholarly journals Competitive Growth of Sulfate-Reducing Bacteria with Bioleaching Acidophiles for Bioremediation of Heap Bioleaching Residue

2020 ◽  
Vol 17 (8) ◽  
pp. 2715 ◽  
Author(s):  
Aung Kyaw Phyo ◽  
Yan Jia ◽  
Qiaoyi Tan ◽  
Heyun Sun ◽  
Yunfeng Liu ◽  
...  
Keyword(s):  
Organic Matter ◽  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Sulfate Reducing Bacteria ◽  
Sulfide Minerals ◽  
Sulfate Reducing ◽  
Heap Bioleaching ◽  
Waste Rocks ◽  
Sulfur Oxidizing ◽  
Reducing Bacteria

Mining waste rocks containing sulfide minerals naturally provide the habitat for iron- and sulfur-oxidizing microbes, and they accelerate the generation of acid mine drainage (AMD) by promoting the oxidation of sulfide minerals. Sulfate-reducing bacteria (SRB) are sometimes employed to treat the AMD solution by microbial-induced metal sulfide precipitation. It was attempted for the first time to grow SRB directly in the pyritic heap bioleaching residue to compete with the local iron- and sulfur-oxidizing microbes. The acidic SRB and iron-reducing microbes were cultured at pH 2.0 and 3.0. After it was applied to the acidic heap bioleaching residue, it showed that the elevated pH and the organic matter was important for them to compete with the local bioleaching acidophiles. The incubation with the addition of organic matter promoted the growth of SRB and iron-reducing microbes to inhibit the iron- and sulfur-oxidizing microbes, especially organic matter together with some lime. Under the growth of the SRB and iron-reducing microbes, pH increased from acidic to nearly neutral, the Eh also decreased, and the metal, precipitated together with the microbial-generated sulfide, resulted in very low Cu in the residue pore solution. These results prove the inhibition of acid mine drainage directly in situ of the pyritic waste rocks by the promotion of the growth of SRB and iron-reducing microbes to compete with local iron and sulfur-oxidizing microbes, which can be used for the source control of AMD from the sulfidic waste rocks and the final remediation.

scholarly journals Effectiveness of Organic Materials as Media in Sulfate Reducing Bacteria Inoculum to Changes on Acid Sulfate Soils

2021 ◽  
Vol 2 (1) ◽  
pp. 45-49
Author(s):  
Mazlina Mazlina ◽  
Asmarlaili S Hanafiah ◽  
A Rauf ◽  
Edy Sigit Sutarta
Keyword(s):  
Organic Matter ◽  
Soil Ph ◽  
Block Design ◽  
Field Capacity ◽  
Nutrient Content ◽  
Acid Sulfate Soils ◽  
Randomized Block Design ◽  
Different Types ◽  
Sulfate Soils ◽  
Reducing Bacteria

Acid sulphate soils (ASS) had low pH, low nutrients availability and also soluble aluminium and iron were high. Inoculum of sulphate reducing bacteria (SRB) which organic matter as media could increased the soil pH, nutrient content and decrease sulfur-total of acid sulfate soils. The research was conducted in a randomized block design with two replications. This study used a randomized block design with two factors and two replications. The first factor was compost inoculum (C) was taken ten treatments from without any  inoculum SRB and 9 treatment with different types and dosages of organic matter as media. The second factor was the water content condition (K) namely of K1: 100% field capacity and K2: 110% field capacity. Different types and dosages of organic matter appear to influence the changes in soil properties (tends to decrease soil sulfate and increase soil pH, and nutrient content levels in soil and plant). Inoculum SRB of palm oil empty bunches and weed gave a higher sulfate reduction compared to C0 (without inoculums) or inoculums with carrier media that used rice straw in water content 100% or 110% field capacity (FC).

scholarly journals EFISIENSI SISTEM LAHAN BASAH BUATAN ALIRAN PERMUKAAN DENGAN VARIASI DEBIT DALAM MENYISIHKAN MANGAN PADA AIR ASAM TAMBANG

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Nopi Stiyati Prihatini ◽  
Chairul Abdi ◽  
Yudha Ajie Pratama ◽  
Ihsan Noor
Keyword(s):  
Acid Mine Drainage ◽  
Constructed Wetland ◽  
Mine Drainage ◽  
Ph Value ◽  
Surface Flow ◽  
Acid Mine Water ◽  
Acid Mine ◽  
Water Ph ◽  
Ph Increase ◽  
High Concentrations

Terbentuknya air asam tambang  (AAT) merupakan salah satu dampak dari adanya aktifitas pertambangan. Air asam tambang memiliki ciri pH asam berkisar antara 3-5, warna air kuning kemerahan, dengan konsentrasi besi (Fe) dan mangan (Mn) yang tinggi. Penyisihan Mn menjadi tantangan tersendiri karena mangan hanya dapat disisihkan jika konsentrasi Fe AAT kurang dari 5 mg/L. Teknologi yang kini dikembangkan untuk menyisihkan Mn air asam tambang adalah sistem lahan basah buatan (LBB). Kinerja LBB dipengaruhi oleh debit AAT. Untuk mengetahui efesiensi penyisihan mangan (Mn) dan nilai pH pada LBB, maka pada penelitian ini digunakan Lahan Basah Buatan Aliran Permukaan (LBB-AP) berdimensi 150cm x 50 cm x 75 cm beraliran kontinyu dengan variasi debit. Pada penelitian ini akan divariasi debit AAT yaitu D0 7,1 mL/menit; D1 8,8 mL/meni; D2 10,5 mL/menit, dan D3 12,2 mL/menit. Hasil penelitian menunjukkan peningkatan pH terbaik pada D0 7,1 mL/menit periode ke 4 dengan nilai pH 4,3. Kemampuan penyisihan Mn terbaik pada LBB D0 7,1 mL/menit periode ke 1 sebesar 3,3 mg/L dengan efisiensi sebesar 62%. Kata Kunci : air asam tambang, lahan basah buatan aliran permukaan, mangan, variasi debit. The formation of acid mine drainage (AAT) is one of the impacts of mining activities. Acid mine water has a characteristic low water pH ranging from 3-5, the color of reddish-yellow water, and has high concentrations of iron (Fe) and manganese (Mn). Mn removal is a challenge because manganese can only be removed if the concentration of Fe AAT is less than 5 mg / L. One of the technologies that can be used to remove Mn from acid mine drainage is the constructed wetland system (CW). CW performance is influenced by AAT debits. To determine the efficiency of removal of manganese (Mn) and the pH value in CW, then in this study used Surface Flow Constructed Wetland (SFCW) with dimensions of 150cm x 50 cm x 75 cm with the continuous flow with variations in debit. In this study, AAT debit will be varied, namely D0 7.1 mL / min; D1 8.8 mL / min; D2 10.5 mL / min, and D3 12.2 mL / min. The results showed the best pH increase at CW D0 7.1 mL/min for the 4th period with a pH value of 4.3. The best Mn removal ability at CW D0 7.1 mL/min in the first period was 3.3 mg / L with an efficiency of 62%. Keywords: Acid mine drainage, Debit variation, Manganese, Surface flow Constructed Wetland.

scholarly journals Microbial Diversity in Anaerobic Sediments at Río Tinto, a Naturally Acidic Environment with a High Heavy Metal Content

2011 ◽  
Vol 77 (17) ◽  
pp. 6085-6093 ◽  
Author(s):  
Irene Sánchez-Andrea ◽  
Nuria Rodríguez ◽  
Ricardo Amils ◽  
José Luis Sanz
Keyword(s):  
Organic Matter ◽  
Mine Drainage ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Extreme Environment ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
High Concentration ◽  
Content Type ◽  
Tinto River ◽  
Reducing Bacteria

ABSTRACTThe Tinto River is an extreme environment located at the core of the Iberian Pyritic Belt (IPB). It is an unusual ecosystem due to its size (100 km long), constant acidic pH (mean pH, 2.3), and high concentration of heavy metals, iron, and sulfate in its waters, characteristics that make the Tinto River Basin comparable to acidic mine drainage (AMD) systems. In this paper we present an extensive survey of the Tinto River sediment microbiota using two culture-independent approaches: denaturing gradient gel electrophoresis and cloning of 16S rRNA genes. The taxonomic affiliation of theBacteriashowed a high degree of biodiversity, falling into 5 different phyla:Proteobacteria,Firmicutes,Bacteroidetes,Acidobacteria, andActinobacteria; meanwhile, all theArchaeawere affiliated with the orderThermoplasmatales. Microorganisms involved in the iron (Acidithiobacillus ferrooxidans,Sulfobacillusspp.,Ferroplasmaspp., etc.), sulfur (Desulfurellaspp.,Desulfosporosinusspp.,Thermodesulfobiumspp., etc.), and carbon (Acidiphiliumspp.,Bacillusspp.,Clostridiumspp.,Acidobacteriumspp., etc.) cycles were identified, and their distribution was correlated with physicochemical parameters of the sediments. Ferric iron was the main electron acceptor for the oxidation of organic matter in the most acid and oxidizing layers, so acidophilic facultative Fe(III)-reducing bacteria appeared widely in the clone libraries. With increasing pH, the solubility of iron decreases and sulfate-reducing bacteria become dominant, with the ecological role of methanogens being insignificant. Considering the identified microorganisms—which, according to the rarefaction curves and Good's coverage values, cover almost all of the diversity—and their corresponding metabolism, we suggest a model of the iron, sulfur, and organic matter cycles in AMD-related sediments.

Using Decomposition Kinetics to Model the Removal of Mine Water Pollutants in Constructed Wetlands

1994 ◽  
Vol 29 (4) ◽  
pp. 219-226 ◽  
Author(s):  
William J. Tarutis ◽  
Richard F. Unz
Keyword(s):  
Organic Matter ◽  
Constructed Wetlands ◽  
Mine Drainage ◽  
Term Treatment ◽  
Decomposition Kinetics ◽  
Dry Density ◽  
Organic Matter Concentration ◽  
Long Term Treatment ◽  
Input Variables ◽  
Reaction Stoichiometry

Although numerous mathematical models have been used to describe decomposition, few, if any, have been used to model the removal of pollutants in constructed wetlands. A steady-state model based on decomposition kinetics and reaction stoichiometry has been developed which simulates the removal of ferrous iron entering wetlands constructed for mine drainage treatment. Input variables for the model include organic matter concentration, reaction rate coefficient, porosity and dry density, and hydraulic detention time. Application of the model assumes complete anaerobic conditions within the entire substrate profile, constant temperature, no additional organic matter input, and subsurface flow only. For these ideal conditions, model simulations indicate that wetlands constructed with readily decomposable substrates rich in organic carbon are initially capable of removing far greater amounts of iron than wetlands built with less biodegradable substrates. However, after three to five years of operation this difference becomes negligible. For acceptable long-term treatment performance, therefore, periodic additions of decomposable organic matter will be required.

scholarly journals Yield of castor bean fertilized with sewage sludge and potassium and magnesium sulphate

2015 ◽  
Vol 19 (8) ◽  
pp. 773-779 ◽  
Author(s):  
Thâmara F. M. Cavalcanti ◽  
Geraldo R. Zuba ◽  
Regynaldo A. Sampaio ◽  
João P. Carneiro ◽  
Ely S. A. de Oliveira ◽  
...  
Keyword(s):  
Organic Matter ◽  
Sewage Sludge ◽  
Castor Bean ◽  
Block Design ◽  
Additional Treatment ◽  
Iron Copper ◽  
Maximum Value ◽  
Randomized Block Design ◽  
N Requirement ◽  
Bean Leaves

<title>ABSTRACT</title><p>The aim of this study was to evaluate the yield and nutrition of castor bean in response to fertilization with sewage sludge and potassium (K) and magnesium (Mg) sulphate. The experiment was carried out from January to July 2011. The treatments, in a randomized block design with three replicates, in a Nitosol, corresponded to a factorial scheme (2 x 4 +1): two doses of K and Mg sulphate combined with four doses of sewage sludge (0, 2.60, 5.20 and 10.40 t ha<sup>-1</sup>, dry basis), applied based on its nitrogen (N) content and the N requirement for the crop and an additional treatment with NPK. The castor bean grain yield fertilized with sewage sludge did not differ from conventional fertilization, with the maximum value achieved at a dose of 7.5 t ha<sup>-1</sup> of sewage sludge. The fertilization with sewage sludge increased zinc and copper levels in the soil to values close to or higher than those in conventional fertilization, without any influence on the concentrations in the leaf. Fertilization with K and Mg sulphate increased the levels of these cations in the soil without affecting the concentrations in the leaves. The fertilization with sewage sludge increased the contents of organic matter, sulfur, zinc, iron, copper and boron in the soil, and manganese and boron in castor bean leaves.</p>

Simultaneous oxidation and reduction treatments of polluted water by a bio-electro reactor

1996 ◽  
Vol 34 (9) ◽  
pp. 101-108 ◽  
Author(s):  
M. Kuroda ◽  
T. Watanabe ◽  
Y. Umedu
Keyword(s):  
Aqueous Solution ◽  
Organic Matter ◽  
Electric Current ◽  
Removal Efficiency ◽  
Concentration Ratio ◽  
Nitrate Removal ◽  
Hydrogen Gas ◽  
Polluted Water ◽  
Simultaneous Oxidation ◽  
Denitrifying Microorganisms

Application of a bio-electro reactor for treatment of various kinds of polluted water was investigated experimentally. Aqueous solution of nitrate, ammonium and/or organic matter were used as synthetic polluted water. Denitrification of the nitrate polluted water without organic matter proceeded effectively by utilizing hydrogen gas produced by electrolysis of water in the reactor. The bio-electro reactor was also available for the treatment of nitrate polluted water containing organic matter when the C/N concentration ratio was up to 1.0 under the condition of 100 mA of applied electric current. The nitrate removal efficiency from nitrate polluted water containing acetate at C/N=1.0 was more than 90% at 5 hours of HRT and 80% even at 2.8 h HRT. For the treatment of ammonium polluted water, nitrification and denitrification proceeded simultaneously in a bio-electro reactor where nitrifying and denitrifying microorganisms were immobilized on the electrodes. The results obtained in this study suggested that the bio-electro reactor system was capable to application for oxidation and reduction treatments of the nitrate and ammonium polluted water.

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