Optimization of the extraction of total flavonoids and antioxidant activity of Alchornea cordifolia leaves using experimental design

This research was conducted to identify the phytochemical compounds and antioxidant activity of pepe leaves (Gymnema reticulatum Br.) crude extract in various solvent types. The experimental design used in this research was a completely randomized design with solvent type treatments consisting of 5 types : aquadest, ethyl acetate 96%, acetone 70%, ethanol 70%, and methanol 95%. The treatment was repeated three times, so that 15 experimental units were obtained. Qualitative data obtained in this research were displayed with tables, while quantitative data were analyzed by variance and if the treatment had a significant effect, then followed by Duncan's test. The results showed that the treatment of solvent types affected phytochemical compounds that was extracted from pepe leaves and had a significant effect (P<0.01) on yield, total phenol, total flavonoids, and antioxidant activity. The best results showed that 70% acetone solvents were able to extract phytochemical compounds from alkaloids, saponins, phenols, flavonoids, and steroids in the form of sterols and had the highest antioxidant activity based on 62.06% free radical inhibition percentage with IC50 values of 200.775 mg/L, yield 32.35%, total phenol 36.14 mgGAE/g extract, and total flavonoids 50.37 mgQE/g extract.

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Quantitative estimation of total phenols, total flavonoids, and antioxidant activity of methanolic extracts of Ephedra gerardiana Wall. ex Stapf across different habitats of cold desert biosphere reserve, trans Himalaya, India

Medicinal Plants - International Journal of Phytomedicines and Related Industries ◽

10.5958/0975-6892.2020.00072.6 ◽

2020 ◽

Vol 12 (4) ◽

pp. 598-608

Author(s):

Deepti Negi ◽

Sher Singh Samant

Keyword(s):

Antioxidant Activity ◽

Quantitative Estimation ◽

Biosphere Reserve ◽

Total Flavonoids ◽

Total Phenols ◽

Cold Desert ◽

Methanolic Extracts

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Antioxidant activity, total flavonoids and volatile constituents of Magonia Pubescens A.St.-Hil

Journal of Medicinal Plants Research ◽

10.5897/jmpr2015.5983 ◽

2015 ◽

Vol 9 (43) ◽

pp. 1089-1097 ◽

Cited By ~ 1

Author(s):

M R Aguiar Marcela ◽

T Santos Kamylla ◽

A Royo Vanessa ◽

S A da Fonseca Francine ◽

V Menezes Elytania ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Flavonoids ◽

Volatile Constituents

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RELATIONSHIP BETWEEN ANTIOXIDANT PROPERTIES AND NUTRITIONAL COMPOSITION OF SOME GALACTOPOIETICS HERBS USED IN INDONESIA: A COMPARATIVE STUDY

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i12.14964 ◽

2016 ◽

Vol 8 (12) ◽

pp. 236 ◽

Cited By ~ 4

Author(s):

Ade Chandra Iwansyah ◽

Rizal M. Damanik ◽

Lilik Kustiyah ◽

Muhammad Hanafi

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Total Phenolics ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Nutritional Composition ◽

Total Flavonoids ◽

Wide Range ◽

Phenolics Content

Objective: The objectives of this research were to evaluate and compare the antioxidant activity; phenolics and flavonoids contents; and chemical composition of some galactopoietic herbs used in Indonesia and to find a possible relationship between antioxidant activity; phenolics, flavonoids and chemical composition of these plants.Methods: A wide range of analytical parameters was studied, including yield; pH; water activity (aw); total color difference; phenolics and flavonoids content; moisture; ash; protein; fat; carbohydrate; fiber; calcium; zinc; magnesium; potassium; iron content; and the data were analyzed by partial least squares (PLS) regression analysis and principal component analysis (PCA) to evaluate the correlation between the parameters and classification of the plants. Folin–cioceltaeu assay, aluminum chloride assay and DPPH scavenging assay were used to evaluate of total phenolics content and free radical scavenging activity of the ethanolic extract of galactopoietic herbs.Results: All of the galactagogue herbs, viz. Plectranthus amboinicus (Lour.) (PA), Sauropus androgynus (SA), Moringa oleifera (MO), and Carica papaya leaves (CP) exhibited good results of TPC, TFC and antioxidant activity. MO contained the highest average of total phenolics content (53.89 mg GAE/g). SA with a ratio of total flavonoids to total phenolics (TF/TP) which is 1.59 contained the highest average of total flavonoids and antioxidant activity (IC50), 43.63 mg QE/g and 126.13 μg/ml, respectively, followed by MO>CA>PA. Based on PLS and PCA analysis, antioxidant activity correlated with fat, protein, phenolics content, flavonoids content and pH, but only the total flavonoids content correlated strongly with antioxidant activity.Conclusion: This provides useful data about the studied relationships and chemical patterns in some galactopoietic herbs used and provides a helpful tool for understanding much more about the interpretation of results from the experiments and the mechanism of biosynthesis in plants.

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Quality of strawberries subjected to pre-harvest UV-C radiation

Pesquisa Agropecuária Brasileira ◽

10.1590/s1678-3921.pab2021.v56.01590 ◽

2021 ◽

Vol 56 ◽

Author(s):

Izabel Camacho Nardello ◽

Rufino Fernando Flores Cantillano ◽

Jardel Araújo Ribeiro ◽

Gerson Kleinick Vignolo ◽

Marcelo Barbosa Malgarim ◽

...

Keyword(s):

Antioxidant Activity ◽

Experimental Design ◽

Vitamin C ◽

Fragaria X Ananassa ◽

Soluble Solids ◽

Soluble Solids Content ◽

Single Factor ◽

Solids Content ◽

Uv C

Abstract The objective of this work was to evaluate the influence of the use of UV-C radiation at an intensity of 0.53 KJ m-2, during strawberry (Fragaria x ananassa) cultivation, on the physicochemical and phytochemical parameters of the fruits. The used experimental design was completely randomized, in a single-factor arrangement with 11, 19, 29, and 39 UV-C applications and without UV-C application. The hue of the fruits was smaller at the end of the study period. pH varied in strawberries that received 39 applications of UV-C, and the soluble solids content differed with 19 applications. Antioxidant activity was lower in fruits with 11, 19, and 29 UV-C applications, whereas vitamin C content was lower only in fruits that received 29 applications. The use of UV-C radiation at an intensity of 0.53 KJ m-2, during strawberry cultivation, affects the physicochemical and phytochemical parameters of the fruits, but does not cause losses in their quality.

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Nutritional, Textural, and Sensory Quality of Aloe Vera Leaf Gel Powder Fortified Plain Cake

J ◽

10.3390/j4030033 ◽

2021 ◽

Vol 4 (3) ◽

pp. 430-443

Author(s):

Dhrubajyoti Singha ◽

Md. Fahad Jubayer ◽

Kumkum Devnath ◽

Delara Akhter ◽

Thottiam Vasudevan Ranganathan ◽

...

Keyword(s):

Antioxidant Activity ◽

Sensory Quality ◽

Aloe Vera ◽

Quality Characteristics ◽

Total Flavonoids ◽

Total Polyphenols ◽

Nutritional Profile ◽

Nutrient Value ◽

Freeze Dried

Aloe Vera leaves have great potential as an economic supplement with an adequate nutritional profile. The current study aimed to fortify plain (loaf) cakes with Aloe Vera leaf gel (AVG) powder. AVG was freeze-dried to produce Aloe Vera powder (ALP), and four plain (loaf) cakes were prepared with different proportions of ALP (0, 4, 6, and 8%). ALP contained significantly (p < 0.05) more protein (22.23 vs. 12.24), ash (19.83 vs. 0.64), and iron (175 vs. 3.05) than refined wheat flour (RWF). Along with total polyphenols and total flavonoids, ALP demonstrated good antioxidant activity. ALP-cakes and RWF-cakes were also evaluated for their nutritional and functional properties. The addition of 6 and 8% ALP to the formulation increased total polyphenols, total flavonoids, and antioxidant activity in plain (loaf) cakes. Hardness and chewiness increased in ALP-cakes but decreased in RWF-cakes, while cohesiveness and springiness decreased in ALP-cakes. In conclusion, the best formulation was a 4% ALP incorporated cake, and ALP can be supplemented in plain cakes at a rate of up to 8% to improve nutrient value. This is the first study to evaluate the quality characteristics of fortified plain (loaf) cakes using ALP.

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PENGARUH WAKTU MASERASI TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN PEGAGAN (Centella asiatica (L.) Urban)

Jurnal Ilmu dan Teknologi Pangan (ITEPA) ◽

10.24843/itepa.2020.v09.i04.p12 ◽

2020 ◽

Vol 9 (4) ◽

pp. 482

Author(s):

Ni Komang Trisna Rahayu ◽

I Dewa Gede Mayun Permana ◽

GA. Kadek Diah Puspawati

Keyword(s):

Antioxidant Activity ◽

Experimental Design ◽

Leaf Extract ◽

Centella Asiatica ◽

Total Flavonoid ◽

Time Duration ◽

Time Treatment ◽

Randomized Design ◽

Completely Randomized Design ◽

Effect On Yield

This research aimed to determine the effect of maceration time on the antioxidant activity of pegagan leaf extract and to determine the best maceration time with highest antioxidant activity of pegagan leaf extract. The experimental design used was Completely Randomized Design with maceration time duration there were 18, 24, 30, 36, 42, and 48 hours. All of the treatment repeated three times to obtained 18 units of experiments. The data analyzed by analyze of variance, if the treatment had a significant effect followed by The Duncan Test. The results showed that the maceration time treatment had a significant effect on yield, total phenolik, total flavonoid, total tannin and antioxidant activity of pegagan leaf extract. The 24 hour maceration time was the best treatment with antioxidant activity 66.67%, IC50 632.82 ppm, a yield of 24.30%, a total phenolik of 57.85 mgGAE / g, a total flavonoid of 105.28 mgQE / g. total tannin 54.09 mgTAE / g.

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