Estimation of total Flavonoids content and Studies on antioxidant activity of different extracts of Cadaba farinosa Forsk by Nitric oxide scavenging and FRAP techniques

2021 ◽  
Vol 13 (03) ◽  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Total Flavonoids ◽  
Total Flavonoids Content

scholarly journals Drying Temperature Effect on Total Phenols and Flavonoids Content, and Antioxidant Activity of Borassus aethiopum Mart Ripe Fruits’ Pulp

2017 ◽  
Vol 6 (2) ◽  
pp. 50 ◽  
Author(s):  
Tagouelbe Tiho ◽  
Jean Claude N'guessan Yao ◽  
Casimir Yao Brou ◽  
Augustin Amissa Adima
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Acetone ◽  
Total Flavonoids ◽  
Distilled Water ◽  
Total Phenols ◽  
Aqueous Methanol ◽  
Drying Temperature ◽  
Total Flavonoids Content ◽  
Main Effects ◽  
Borassus Aethiopum

We assessed the total phenols content (TPC) by Folin-Ciocalteux method, total flavonoids content (TFC), and antioxidant activity (AOA) using ABTS radical cation of Borassus aethiopum Mart ripe fruits; uniquely its pulp was considered. Fresh and dried at 40, 50, 60, 70 and 80oC pulps were analyzed, through maceration and decoction, and distilled water (DW), aqueous acetone (DW+Aceto, DW/acetone, 30/70, v/v) and aqueous methanol (DW+Meth) prepared in the same way were used. Though 80oC product extracted through decoction delivered the highest extracts of TPC with DW, DW+Aceto and DW+Meth, 654.434, 780.066 and 729.934 mg GAE/g respectively; we could not retain it as the best. Very likely, the sugars were caramelizing and the derived product had an intense brown color. Whereas, at 70oC, TPCs were 447.866, 337.334 and 327.066 mg GAE/g, respectively, by decoction and in the same above solvents order. The best extract obtained with DW was statistically different from that of DW + Aceto and DW + Meth, which were not significantly different from each other, with Duncan least squares means comparison test in a confidence interval of 99%. The corresponding TFCs and AOAs were (59.34, 68.34, and 51.66 mg QE/g) and (0.4404, 0.52088, and 0.6524 µmol TE/g). The flour has a nice chocolate color. Except 60oC, an increase of the drying temperature leaded to a rising amount of TPC. Taking each factor, the extraction mode and drying temperature were main effects for extracts (p< 0.0001). The overall regression analysis showed significant correlation coefficient between TPC and AOA, with R2=0.8394.

scholarly journals Variation of Bioactive Compounds and Antioxidant Activity of Jujube (Ziziphus jujuba) Fruits at Different Stages of Ripening

2018 ◽  
Vol 46 (1) ◽  
pp. 134-137 ◽  
Author(s):  
Sina COSMULESCU ◽  
Ion TRANDAFIR ◽  
Violeta NOUR ◽  
Gheorghe ACHIM ◽  
Mihai BOTU ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Organic Acids ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Ripening Stage ◽  
Total Flavonoids Content ◽  
Ziziphus Jujuba

Fruits of two jujube (Ziziphus jujuba Mill.) cultivars were collected at four stages of ripening and analyzed in terms of chemical compounds. The cultivars were grown under the climatic conditions of the Sub-Carpathian area Ramnicu-Valcea in Romania (45°6′17″N, 24°22′32″E). Total phenols content, total flavonoids content, organic acids from jujube fruits and related antioxidant activities were investigated during the ripening stages. The antioxidant activity of alcoholic extracts was investigated by the 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. Total phenolic content was determined by using the Folin-Ciocalteu assay, while determination of flavonoids was done by aluminium nitrate colorimetric method, and organic acids were separated by reversed phase chromatography. The results obtained have indicated significant differences (p < 0.05) found in bioactive compounds and antioxidant activity, depending on cultivars and ripening stage. Total phenolic content ranged from 1634.44 to 472.38 mg GAE/100 g in methanolic extracts of the two varieties studied, while total flavonoids content ranged from 19.9 to 48.5 mg QE/100 g. As regards the influence of ripening stage, the results show that the highest antioxidant activity was recorded in the stage of white maturity. The impact of maturity stage was significant on organic acid distribution of jujube extracts, and ascorbic acid is the major organic acid found in jujube fruits, followed by malic acid and lactic acid. Jujube fruits are rich in bioactive compounds and can be a complement in human’s healthy eating.

scholarly journals Antioxidant Activity of Extract from Ultrasonic-Assisted Extraction of Durian Peels

2018 ◽  
Vol 5 (2) ◽  
Author(s):  
Bambang Kunarto ◽  
Ely Yuniarti Sani
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolics ◽  
Extraction Process ◽  
Extraction Time ◽  
Total Flavonoids ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Total Flavonoids Content ◽  
Phenolics Content ◽  
Ultrasonic Assisted Extraction

The increase in durian production results in the accumulation of durian peel waste. The bioactive component of durian peel has the potential to be used as an antioxidant. Thus, there is a need to carry out an extraction process to obtain  bioactive compounds from durian peel. However, conventional extraction methods cause damage to phenolic compounds due to oxidation, hydrolysis and ionization reactions during the extraction process. Therefore, durian peel extraction was carried out using ultrasonic assisted extraction method (UAE) in this study. The purpose of this study was to investigate the effect of varying ratios of durian peel to ethanol solvents and extraction time on the yield, total phenolics content, total flavonoids content and antioxidant activity. The results of the research data were analyzed using a two-factor completely randomized design, which included variations of the ratio of durian peel to ethanol solvents and extraction time The Duncan’s New Multiple Range Test (DNMRT) was carried out as a follow up test to determine the differences in each treatment at a significance level of 0.05. The results showed that the best treatment for peel extraction using ultrasonic assisted extraction was a 1: 9 ratio of durian peel to ethanol at an extraction time of 20 minutes. The extraction of durian peel under this condition gave the highest yield of 12.77 ± 0.16%, antioxidant activity (IC50) of 38.33 ± 0.12 ppm, total phenolic content of 63.30 ± 0.08 mgGAE / g and total flavonoids content of 47.53 ± 0.48 mgQE / g. In addition, total phenolics content and total flavonoid content showed a strong correlation to the antioxidant activity of durian peel extract.

scholarly journals HPLC profiling of selected phenolic acids and flavonoids in Salvia eigii, Salvia hierosolymitana and Salvia viridis growing wild in Jordan and their in vitro antioxidant activity

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9769
Author(s):  
Hala I. Al-Jaber ◽  
Ashok K. Shakya ◽  
Zaha A. Elagbar
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Rosmarinic Acid ◽  
Salvianolic Acid B ◽  
Total Flavonoids ◽  
High Concentration ◽  
Total Flavonoids Content ◽  
Concentration Levels ◽  
In Vitro Antioxidant Activity

Background Salvia eigii., Salvia hierosolymitana and Salvia viridis are native to the Mediterranean region, and are used in traditional medicine for the treatment of many ailments. In the current investigation, the methanolic extracts obtained from the air dried aerial parts of S. eigii, S. hierosolymitana and S. viridis from Jordan were screened for their total phenolics content (TPC), total flavonoids content (TFC) and their in vitro antioxidant activity. Additionally, the presence of four bioactive phenolic acids including gallic acid, caffeic acid, rosmarinic acid and salvianolic acid B and other seven flavonoids including luteolin-7-O-glucoside, apigenin, apigenin-7-O-glucoside, rutin, nariginin, hesperidin and quercetin was determined using Liquid chromatography-Electron Spray Ionization-Tandom Mass Spectrometry (LC-ESI-MS/MS). Methods Antioxidant activity of the obtained three extracts were examined via the DPPH•, ABTS• + radical scavenging methods in addition to Ferrous Ion Chelating (FIC) effect. TFC and TPC of the extracts were measured using the aluminum chloride colorimetric method and the Folin-Ciocalteau method, respectively. The presence and concentration of the selected 11 compounds was further determined through LC-ESI-MS/MS. Results The results indicated that three Salvia species had high total flavonoids content expressed in mg quercetin/g dry extract (S. heirosolymitana: 770.85 ±  5.26; S. eigii: 520.60 ±  6.24, S. viridis: 311.36 ±  4.41). S. heirosolymitana had the highest DPPH• activity (0.184 ±  1.22 × 10−2 mg/ml) and FIC effect (0.354 ±  0.018 mg/ml). S. heirosolymitana had slightly higher ABTS• + scavenging activity than S. eigii (0.176 ±  1.16 × 10−2 mg/ml; 0.183 ±  0.031 mg/ml, respectively). All 11 compounds were detected in the extracts of the three Salvia species. Luteolin-7-O-glucoside was detected in high concentration levels in the three species (1756.73, 21651.36, and 26125.14 mg/kg dry plant; S. eigii, S. hierosolyimitana and S. viridis, respectively), yet rosmarinic acid had the highest contribution to both S. hierosolymitana (27124.93 mg/kg) and S. eigii (15783.33 mg/kg). Notably, S. hierosolymitana and S. viridis contained salvianolic acid B (896.11; 890.9 mg/kg). Conclusions The three Salvia species exhibited good antioxidant activity, especially S. heirosolymitana due to its high TPC, TFC, and the presence of high concentration levels of romarinic acid and other phenolic acids and flavonoids. This is the first phytochemical and antioxidant evaluation of S. eigii, S. hierosolymitana and S. viridis from Jordan. Prior to this investigation, no phytochemical investigation on S. eigii was reported.

scholarly journals Pigments, Polyphenols and Antioxidant Activity of Leaf Extracts from Four Wild Rose Species Grown in Sicily

2018 ◽  
Vol 46 (2) ◽  
pp. 402-409 ◽  
Author(s):  
Francesca D'ANGIOLILLO ◽  
Michele Massimo MAMMANO ◽  
Giancarlo FASCELLA
Keyword(s):  
Antioxidant Activity ◽  
Morphological Characteristics ◽  
Natural Antioxidants ◽  
Total Flavonoids ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Dpph Method ◽  
Total Anthocyanins ◽  
Total Flavonoids Content ◽  
Promising Source

Leaf methanolic extracts of four wild Rose species (Rosa canina L., R. corymbifera Borkh., R. micrantha Borrer ex Sm and R. sempervirens L.) grown in Sicily were analysed in order to evaluate differences among indigenous roses which could be used such as source of bioactive compounds and natural antioxidants. Leaves were harvested during two different periods (June and October) and their morphological characteristics were described. Leaf content of chlorophylls, total anthocyanins, total flavonoids, total polyphenols (Folin-Ciocalteu method) and antioxidant activity (DPPH method) were determined. Results showed that the contents of chlorophylls and carotenoids were higher in leaves of all species harvested in June than in those harvested in October. On the contrary, total anthocyanins of R. micrantha and R. corymbifera leaves were higher in October. Total polyphenol content was higher in leaves of all species were harvested in October. No difference in total flavonoids content was recorded between the two harvest periods but only among the four species. Antioxidant activity was generally high in leaf extracts of all species in both periods and, particularly, in R. micrantha leaves harvested in June. These outcomes seem to support the assessment that leaves harvested from some Sicilian wild roses may be used as promising source of healthy compounds and that their content varies with the species and harvest period.

scholarly journals Glucosinolate profiles by HPLC-DAD, phenolic compositions and antioxidant activity of Eruca vesicaria longirostris: Impact of plant part and origin

2016 ◽  
Vol 5 (5) ◽  
pp. 528-539
Author(s):  
Saoussen Bouacida ◽  
Hayet Ben Haj koubaier ◽  
Ahmed Snoussi ◽  
Marie Laure Fauconnier ◽  
Nabiha Bouzouita
Keyword(s):  
Antioxidant Activity ◽  
Statistical Analysis ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Plant Part ◽  
The Other ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Flavonoids Content ◽  
Seed Extracts

The glucosinolate profiles, phenol and flavonoid contents and the antioxidant activity of Eruca vesicaria longirostris were studied for different organs and origins. Eleven desulpho-glucosinolates (DS-GLSs) were isolated and quantified by lipid chromatography- DAD. Similarity between profiles was obtained. Total DS-GLS content, expressed as sinigrin equivalents (SE) revealed a certain variabilily ranging between (76.07-45.61), (27.01-13.53), (4.52 -18.01), (9.39-3.37) and (1.16-13.99) µmol /g DW for seeds, flowers, leaves, roots and stems, respectively. Results showed that seeds are rich in phenolics as they contain highest amounts of phenolics ranging from 27.6±0.5 to 33.47±0.5 mg GAE/g extract as compared to all other parts. Leaves and flowers had a significantly higher total phenolic content than stems and roots in all samples (p < 0.05). According to statistical analysis, the investigated seed extracts with values between (16.20±0.10-18.50±0.10 mg QE/g) exhibited the highest total flavonoids content, followed by leaves (13.00±0.40-15.80±0.30mg QE/g), flowers (10.40±0.40-12.90±0.90 mg QE/g) and stems (7.80±0.20- 9.80±0.70 mg QE/g). Antioxidant activity tested by DPPH, ABTS and FRAP assays, was higher for seeds, leaves and flowers than the other studied organs. These organs were characterized by a significantly high content in glucoerucin, nasturtin and epiprogroitrin, respectively.

scholarly journals Bioactive Content of Rose Hips of Different Wildly Grown Rosa dumalis Genotypes

2016 ◽  
Vol 44 (2) ◽  
pp. 472-476 ◽  
Author(s):  
Sevket ALP ◽  
Sezai ERCISLI ◽  
Tunde JURIKOVA ◽  
Ozlem CAKIR ◽  
Sadiye GOZLEKCI
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Fresh Weight ◽  
Total Carotenoids ◽  
Treatment And Prevention ◽  
Bioactive Content ◽  
Total Flavonoids Content ◽  
Genotype E

A rose hip is the fruit of a rose plant and mostly belongs to Rosa canina and Rosa dumalis. These species are genuine found as wild in natural conditions, while in some countries are cultivated for their hips that are picked and used in a wide variety of preparations. Because they contain a variety of antioxidants, carotenoids, flavonoids, polyphenols, leucoanthocyanins and catechins, rose hips are considered to be a good cancer preventative. The Rosa genus has been the focus of many recent studies due to its potential benefic effects on treatment and prevention of several diseases. However, there are few in vitro studies concerning its composition and antioxidant capacity. The aim of the present study was to determine bioactive content, including ascorbic acid, total phenolics, total flavonoids, total carotenoids and antioxidant activity of Rosa dumalis genotypes growing wild in Erzurum province located eastern Anatolia. The results revealed that fruits of Rosa dumalis different genotypes were rich rich in terms of vitamin C, which ranged from 402 to 511 mg/100 g fresh weight base. The total phenolic content varied from 297 to 403 mg/100 g fresh weight. The genotype 'E-09' had the highest total flavonoids content (229 mg quercetin equivalent/100 g fresh weight) and the lowest value was attributed to genotype 'E-04' with 143 mg/ quercetin equivalent/100 g fresh weight. The antioxidant activity of the genotypes was between 12.9-28.6 μg Trolox/ml samples. The results revealed that there was enough diversity among Rosa dumalis genotypes for bioactive content and promising genotypes, with high bioactive content, were determined, which can become study material for future breeding activities.

scholarly journals Bioactive Compounds, Antioxidant Activity and Nutritional Quality of Different Culinary Aromatic Herbs

2017 ◽  
Vol 45 (1) ◽  
pp. 179-184 ◽  
Author(s):  
Violeta NOUR ◽  
Ion TRANDAFIR ◽  
Sina COSMULESCU
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Acid Content ◽  
Nutritional Quality ◽  
Radical Scavenging ◽  
Ascorbic Acid Content ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Flavonoids Content ◽  
Aromatic Herbs

In the present work, four of the most widely used culinary herbs (parsley, dill, lovage and celery leaves) in the Romanian traditional cuisine were assessed for some nutritional quality parameters (moisture content, titratable acidity, ascorbic acid content and mineral content), total phenolics content, total flavonoids content and antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. In addition, high-performance liquid chromatography (HPLC) coupled with diode-array detection was applied for the identification and quantification of some individual phenolic compounds in the selected aromatic herbs. Although parsley showed the highest ascorbic acid content (206.32 mg/100 g fw), it registered the lowest antioxidant activity. Fresh lovage recorded the highest total phenolic content (577.04 mg GAE/100 g), total flavonoids content (298.38 mg QE/100 g) and antioxidant activity, followed by dill and celery leaves. Among the identified phenolic acids, ellagic acid was predominant in lovage and celery leaves whereas sinapic and vanillic acids were prevalent in dill. Among flavonoids, myricetin was predominant in parsley and celery leaves whereas rutin was predominant in dill and lovage.

scholarly journals Phytochemical composition and antioxidant activity of extracts of some medicinal plants in Tunisia

2016 ◽  
Vol 4 (2) ◽  
pp. 159
Author(s):  
Khaled Athmouni ◽  
Taheni Belghith ◽  
Abdelfattah El Fek ◽  
Habib Ayadi
Keyword(s):  
Vitamin D ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Mineral Analysis ◽  
Phytochemical Composition ◽  
Aerial Parts ◽  
Total Flavonoids Content

The aim of this study was to investigate the chemical composition of the best known plants biomarkers from the locality of Gafsa (Tunisia). Majority of investigated plants had high levels of phenolics and exhibited noteworthy antioxidant activity. The plants showing the highest antioxidant activities were P. angustifolia, T. garganica, G. alypum and L. multifida. P. angustifolia demonstrated by far the most potent antioxidant activity, as well as total phenolic and total flavonoids content. In flavonoid structure of the aerial parts of the related species, different amounts of rutin, myricetin, morin, quercetin and kaempferol together with ergosterol, stigma sterol and b-sitosterol as phytosterols, vitamin D, vitamin K, a-tocopherol and retinol as vitamins, and arabinose, fructose, glucose, sucrose and maltose as sugars were found. The mineral analysis was related to the existence of Ca, Na, K, Fe, Mn, Zn and Mg with different amounts in all of the nine tested species.

scholarly journals Estimation of flavonoids and screening of in vitro antioxidant activity of various extracts of aerial parts of Blepharis maderaspatensis by hydroxyl radical and total antioxidant activity

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1204-1209
Author(s):  
Jambula Dinesh Babu ◽  
Bhuvaneswari Santharam ◽  
Kottai Muthu Arumugam
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Bioactive Compound ◽  
Antioxidant Potential ◽  
Total Flavonoids ◽  
Total Antioxidant Activity ◽  
Aerial Parts ◽  
Total Antioxidant ◽  
Total Flavonoids Content

Antioxidants are substances that hamper or inhibit the oxidation of oxidizable substrates in the cells. The aerial parts of Blepharis maderaspatensis dry powder was extracted with various solvents (PE, EA and methanol) through Soxhlet extractor. The aerial parts of different concentrates (Pet.ether, ethyl acetate and methanol) of Blepharis maderaspatensis was evaluated for its in-vitro antioxidant potential by hydroxyl radical, total antioxidant activity taking ascorbate used as standard for the both methods and total flavonoids content was estimated as equivalent to rutin. The methanolic concentrates of Blepharis maderaspatensis & standard exhibited antioxidant potential possessing IC50 210µg/ml & 62µg/ml (hydroxyl radical) 200µg/ml & 57µg/ml (Total antioxidant activity) respectively. An IC50 value was originate that methanolic concentrates of Blepharis maderaspatensis more efficient in hydroxyl radical, total antioxidant activity compared EA & PE concentrates. The methanolic and EA concentrates of Blepharis maderaspatensis showed the total flavonoids content (11.230±0.22 and 2.858±0.56 mg of rutin equivalent/g of powder) respectively. The difference in scavenging potential of the extracts can be due to variation in the percentage of bioactive compound flavonoids present in methanolic extracts. Invitro antioxidant studies obviously show methanolic concentrates of Blepharis maderaspatensis have better antioxidant activity due to the presence of total flavonoids content. The findings from the study reveal that the antioxidative potentials of methanolic concentrates Blepharis maderaspatensis could serve as free radical inhibitors, possibly acting as natural antioxidants.

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