THE EFFECT OF CULTURE MEDIA TYPE AND PLANT GROWTH REGULATORS ON CALLUS INDUCTION OF OIL PALM (Elaeis guineensis Jacq) PISIFERA TYPE

Oil palm has a high economic value since it is one of the highest vegetable oil-producing plants compared to other oil-producing plants. The provision of good planting material for oil palm plantations is one of the determining factors to increase productivity. The parent Pisifera type of oil palm is important to develop the high-yielding oil palm Tenera varieties. The provision of oil palm material could be done through generative system with seeds and also could be done through vegetative approaches using tissue culture techniques. It aims to get plants that are genetically the same as their parents (true to type). The purpose of this study was to see the effect of different type of tissue culture media and plant growth regulators (PGR’s) on the callus induction stage of Pisifera type oil palm. The results show that the treatment using Murasige and Skoog (MS) culture media is able to give a better effect when compared to the use of Eeuwens and Blake (Y3) basic media. While the use of PGR treatment (H6 = NAA 6 mg/l + 2.4-D 0.5 mg/l) showed a callus growth percentage of 76%, which was better than (H1 = Kin 0.1 mg/l + 2.4-D 100 mg/l) by 42%.

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Effect of culture media, plant growth regulators and genotypes on growth and developmental stages of oil palm (Elaeis guineensis Jacq.) zygotic embryos

Indian Journal of Agricultural Research ◽

10.18805/a-5129 ◽

2019 ◽

Author(s):

D.S. Sparjanbabu ◽

P. Naveen Kumar ◽

M.S.R. Krishna ◽

D. Ramajayam ◽

B. Kalyana Babu ◽

...

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Seed Production ◽

Growth Regulators ◽

Oil Palm ◽

Elaeis Guineensis ◽

Culture Media ◽

Zygotic Embryos ◽

Elaeis Guineensis Jacq

Oil palm (Elaeis guineensis Jacq.) has become the largest traded vegetable oil in the world due to its innumerable uses and its huge economic importance. Which increases the requirement of quality seedling but the conventional seed production limits the commercial seed production with its long germination period and low germination rate. In this context in vitro germination of zygotic embryo could be a promising alternative. Hence in this study the effect of culturing media, plant growth regulators and genotypes were assessed on in vitro regeneration and plantlet development of oil palm zygotic embryos. Zygotic embryos of four elite genotypes were cultured on MS, N6 and Y3 media, with or without plant growth regulators (NAA, BA and GA3-0.2mg/lt). There was no effect of culture media, plant growth regulators and genotypes on germination of zygotic embryos. Whereas on the growth and development of in vitro plantlets, there was a significant effect of culture media and genotypes. Where culture media N6 performs well and Y3 has shown more abnormality. In case of genotypes, genotypes G1 and G2 shown better performance when compared to genotypes G3 and G4. The effect of plant growth regulators on germination and plant growth was non significant.

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The possibility of using Glycyrrhiza glabra L. Callus extracts as an alternative to plant growth regulators in plant tissue culture experiments

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2008.2.1.30 ◽

2008 ◽

Vol 2 (1) ◽

pp. 71-80

Author(s):

Kadhim M. Ibrahim ◽

Ashwaq S. Abed ◽

E. A. Abbod ◽

Kholood W. Alsamarae ◽

Nabeel K. ALani

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Callus Induction ◽

Callus Tissue ◽

Culture Media ◽

Water Extract ◽

Shoot Proliferation ◽

Soya Bean ◽

Tissue Extracts

Liquorice plant is considered one of the important medicinal and economical plants. It is rich with many compounds, minerals, vitamins, and even plant hormones. This research is aimed to study the possibility of using callus tissue extracts as an alternative to plant growth regulators added to the culture media. A factorial experiment was implemented to find out the appropriate combination between 2, 4-D and BA for callus induction on Liquoricenode explants. It was found that a combination of 2 mg/l 2, 4-D with 2.5 mg/l BA is the best one for callus induction and maintenance using MS medium. Water and alcoholic extracts were prepared from callus tissue at concentrations (0, 2, 4, 6, 8 or 10) ml/l then added to culture medium as an alternative to plant growth regulators. The effect of these concentrations on growth and development of tissues and organs for some plants was studied using soya bean, potato and wheat plants for this purpose. Results showed that water extract induced shoot proliferation from potato single nodes. Both types of extracts increased soya bean callus fresh weight significantly. It was found also that water extract was more effective than alcoholic one in increasing vegetative and root parts in germinating wheat seeds.

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Effects of different plant growth regulators on in vitro callus induction in physic nut (Jatropha curcus L.)

Journal of Applied and Natural Science ◽

10.31018/jans.v7i1.559 ◽

2015 ◽

Vol 7 (1) ◽

pp. 30-37 ◽

Cited By ~ 2

Author(s):

Sunil Kumar ◽

Virendra Kumar ◽

Manoj Kumar Sharma ◽

Narendra Kumar ◽

Anil Kumar ◽

...

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Callus Induction ◽

Culture Media ◽

Nodal Segments ◽

Leaf Lamina ◽

Physic Nut ◽

Cotyledonary Nodes

Jatropha (Jatropha curcas L.) is an oil bearing crop growing in tropical and subtropical parts of the world. The present study was undertaken to investigate the effects of different plant growth regulators on in vitro callus induction in physic nut (J. curcus). In the present study, it was observed that all the explants viz., leaf lamina, petioles, nodal segments and cotyledonary nodes showed good callus induction responses on various culture media thus tried. Leaf lamina and petioles showed 100.0% callus induction responses on different MS media supplemented with auxins and cytokinins alone or in combinations whereas, nodal segments and cotyledonary nodes showed maximum 89.6% and 83.9% callus induction respectively. The presence of 2, 4-D in culture media with auxins or cytokinins was essential for good callus growth. Among different explants tried, leaf lamina was the best responding explants and MS-13 media supplemented with 5×10-6 M NAA and 10-5 M 2, 4-D is the best callusing and growth supporting medium. However, the regenerative competence of the callus tissues can differ depending on the type of explants used because certain types of plant tissues have more favorable regeneration responses than others. Callus induction rate from all explant types was highest than other reports. The results obtained in the present study would facilitate the high callus induction and regeneration responses in J. curcus for its improvement using biotechnological tools.

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Effects of Plant Growth Regulators, Light Conditions and Culture Media on Multiple Shoot and Microtuber Formation in the Tissue Culture of Ise-imo (Dioscorea opposita)

Japanese Journal of Crop Science ◽

10.1626/jcs.77.481 ◽

2008 ◽

Vol 77 (4) ◽

pp. 481-488

Author(s):

Takanari Sakai ◽

Katsu Imai

Keyword(s):

Tissue Culture ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Culture Media ◽

Multiple Shoot ◽

Light Conditions ◽

Dioscorea Opposita

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Application of Plant Extracts in Micropropagation and Cryopreservation of Bleeding Heart: An Ornamental-Medicinal Plant Species

Agriculture ◽

10.3390/agriculture11060542 ◽

2021 ◽

Vol 11 (6) ◽

pp. 542

Author(s):

Dariusz Kulus ◽

Natalia Miler

Keyword(s):

Tissue Culture ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Plant Extracts ◽

The Other ◽

White Gold ◽

Natural Extracts ◽

Other Hand ◽

The Impact

Lamprocapnos spectabilis (L.) Fukuhara (bleeding heart) is valued both in the horticultural and pharmaceutical markets. Despite its great popularity, information on the in vitro tissue culture technology in this species is limited. There is also little knowledge on the application of plant extracts in the tissue culture systems of plants other than orchids. The aim of this study is to compare the utility of traditional plant growth regulators (PGRs) and natural extracts—obtained from the coconut shreds, as well as oat, rice, and sesame seeds—in the micropropagation and cryopreservation of L. spectabilis ‘Gold Heart’ and ‘White Gold’. The biochemical analysis of extracts composition is also included. In the first experiment related to micropropagation via axillary buds activation, the single-node explants were cultured for a 10-week-long propagation cycle in the modified Murashige and Skoog medium fortified either with 1.11 µM benzyladenine (BA) and 1.23 µM indole-3-butritic acid (IBA) or with 10% (v/v) plant extracts. A PGRs- and extract-free control was also considered. In the cryopreservation experiment, the same 10% (v/v) extracts were added into the medium during a seven-day preculture in the encapsulation-vitrification cryopreservation protocol. It was found that the impact of natural additives was cultivar- and trait-specific. In the first experiment, the addition of coconut extract favoured the proliferation of shoots and propagation ratio in bleeding heart ‘Gold Heart’. Rice extract, on the other hand, promoted callus formation in ‘White Gold’ cultivar and was more effective in increasing the propagation ratio in this cultivar than the conventional plant growth regulators (4.1 and 2.6, respectively). Sesame extract suppressed the development of the explants in both cultivars analysed, probably due to the high content of polyphenols. As for the second experiment, the addition of plant extracts into the preculture medium did not increase the survival level of the cryopreserved shoot tips (sesame and oat extracts even decreased this parameter). On the other hand, coconut extract, abundant in simple sugars and endogenous cytokinins, stimulated a more intensive proliferation and growth of shoots after rewarming of samples. Analysing the synergistic effect of conventional plant growth regulators and natural extracts should be considered in future studies related to L. spectabilis.

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The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

Notulae Scientia Biologicae ◽

10.15835/nsb335931 ◽

2011 ◽

Vol 3 (3) ◽

pp. 97-100

Author(s):

Naimeh SHARIFMOGHADAM ◽

Abbas SAFARNEJAD ◽

Sayed Mohammad TABATABAEI

Keyword(s):

Tissue Culture ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Base Material ◽

Culture Technique ◽

Induction Medium ◽

Root Induction ◽

Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

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