scholarly journals Roles of culture media and oxygen transfer in the scale-up from shake flasks to pneumatic bioreactor of the plant growth-promoting bacterium Rhizobium phaseoli

2021 ◽  
Vol 20 (2) ◽  
pp. 1093-1111
Author(s):  
R.I. García-Cabrera ◽  
◽  
N.A. Valdez-Cruz ◽  
A. Daniel-Vázquez ◽  
A. Blancas-Cabrera ◽  
...  
Keyword(s):  
Plant Growth ◽  
Oxygen Transfer ◽  
Culture Media ◽  
Scale Up ◽  
Rhizobium Phaseoli ◽  
Plant Growth Promoting ◽  
Shake Flasks ◽  
Growth Promoting

Scale-up from shake flasks to pilot-scale production of the plant growth-promoting bacterium Azospirillum brasilense for preparing a liquid inoculant formulation

2013 ◽  
Vol 97 (22) ◽  
pp. 9665-9674 ◽  
Author(s):  
Mauricio A. Trujillo-Roldán ◽  
Norma A. Valdez-Cruz ◽  
César F. Gonzalez-Monterrubio ◽  
Eduardo V. Acevedo-Sánchez ◽  
Carlos Martínez-Salinas ◽  
...  
Keyword(s):  
Plant Growth ◽  
Azospirillum Brasilense ◽  
Scale Up ◽  
Pilot Scale ◽  
Scale Production ◽  
Pilot Scale Production ◽  
Plant Growth Promoting ◽  
Shake Flasks ◽  
Growth Promoting ◽  
Inoculant Formulation

scholarly journals Effect of plant growth-promoting rhizobacterial composite culture on the growth of chickpea seedlings

Biologija ◽  
2018 ◽  
Vol 64 (2) ◽  
Author(s):  
Jitendra Nag ◽  
Biplab Dash ◽  
Anup Kumar Singh ◽  
Tapas Chowdhury ◽  
Shyam Bihari Gupta ◽  
...  
Keyword(s):  
Plant Growth ◽  
Mixed Culture ◽  
Culture Media ◽  
Culture Collection ◽  
Pot Experiment ◽  
Seedling Test ◽  
Departmental Culture ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Traits

In the present study, 20 soil and plant samples from 13 villages of Raipur, Durg, and Balod District of Chhattisgarh (India) were collected from chickpea fields. From these samples, a total of 86 isolates including 16 Rhizobium, 40 Azotobacter, 29 Azosprillum, and one PSB were obtained on selective culture media. All the isolates were screened for their plant growth-promoting traits. Three (GmR8, ASL3 & ASL4) out of 86 were finally selected for further studies. One Azotobacter isolate, i.e., Azo137, was selected from the departmental culture collection. Finally, four isolates including GmR8 (Rhizobium), ASL3, ASL4 (Azospirillum), and Azo137 were selected for composite culture formulations. GmR and ASL4 were siderophore-producing isolates, whereas ASL3 and Azo137 were IAA producer along with their ability to fix nitrogen. Five composite cultures were prepared randomly and tested for effect on the growth of chickpea (the seedling test and the pot experiment). Among all the composite culture groups, C2 (GmR8, Azo137, ASL4) significantly increased the root (10.84 cm) and shoot (8.10 cm) length, whereas biomass (3.60 g) was the highest in the case of C1 (GmR8, Azo137, ASL3, ASL4) of seedlings as compared to the control (6.80 cm, 2.60 cm, and 3.30 g, respectively). Overall, the study revealed a better performance of composite or mixed culture over individual bacteria.

scholarly journals Amelioration of Saline Stress on Chia (Salvia hispanica L.) Seedlings Inoculated With Halotolerant Plant Growth-Promoting Bacteria Isolated From Hypersaline Environments

2021 ◽  
Vol 3 ◽  
Author(s):  
María Florencia Yañez-Yazlle ◽  
Neli Romano-Armada ◽  
Verónica Beatriz Rajal ◽  
Verónica Patricia Irazusta
Keyword(s):  
Plant Growth ◽  
Culture Media ◽  
Saline Stress ◽  
Plant Growth Promoting Bacteria ◽  
Initial Growth ◽  
Hypersaline Environments ◽  
Salvia Hispanica ◽  
Plant Growth Promoting ◽  
Pgp Activities ◽  
Growth Promoting

The rhizosphere and microbiome of halotolerant plants could be crucial for alleviating salinity stress during plant growth. The aims of this work were (1) to isolate bacteria from rhizosphere and bulk soil samples from the Salar del Hombre Muerto (Catamarca, Argentina), (2) to characterize different plant growth-promoting (PGP) activities produced by these bacterial isolates, and (3) to evaluate their effect on the initial growth of chia (Salvia hispanica L.) under saline stress. A total of 667 microorganisms were isolated, using different culture media with NaCl, and their abilities for nitrogen fixation, phosphate solubilization, siderophores production, and indole-3-acetic acid production were evaluated. Thirteen strains were selected for showing all the tested PGP activities; they belonged to the genera Kushneria, Halomonass, Pseudomonas, Planomicrobium, and Pseudarthrobacter. The strains Kushneria sp. and Halomonas sp. showed the highest salinity tolerance (from 50 to 2,000 mM NaCl) and biomass and biofilm production. Chia seeds were treated with six of the first 13 selected strains to evaluate their plant growth-promoting effect under saline stress (without and with 50 and 100 mM NaCl). Halomonas sp. 3R.12 and Kushneria sp. T3.7 produced heavier seedlings with a balanced shoot/root length ratio, while Pseudomonas sp. AN23 showed the best effect upon chia seedlings, with a morphological response similar to non-stressed seedlings. On the other hand, seedlings displayed no responses when inoculated with Planomicrobium sp. 3S.31 and Pseudarthrobacter sp. ER25. This study contributes to the knowledge on microorganisms from hypersaline environments as plant growth promoters for their use in the production of salt-sensitive crops, among other potential uses.

scholarly journals Bacteria Isolated from Wastewater Irrigated Agricultural Soils Adapt to Heavy Metal Toxicity While Maintaining Their Plant Growth Promoting Traits

2021 ◽  
Vol 13 (14) ◽  
pp. 7792
Author(s):  
Abdul Wahab Ajmal ◽  
Saleha Saroosh ◽  
Shah Mulk ◽  
Muhammad Nadeem Hassan ◽  
Humaira Yasmin ◽  
...  
Keyword(s):  
Plant Growth ◽  
Agricultural Soils ◽  
Culture Media ◽  
Growth Promotion ◽  
Metal Resistance ◽  
Rrna Gene ◽  
Gene Sequence Analysis ◽  
Plant Growth Promoting ◽  
Growth Promoting

The present study explored the plant growth promotion and bioremediation potential of bacteria inhabiting wastewater irrigated agricultural soils. Thirty out of 75 bacterial isolates (40%), 29/75 (39%) and 28/75 (37%) solubilized Zn, K and PO4 during plate essays respectively. Fifty-six percent of the isolates produced siderophores, while 30% released protease in vitro. Seventy-four percent of bacteria resisted Pb, Ni and Cd at various concentrations added to the culture media plates. Sixteen out of 75 (26%) isolates were able to fix N in Nbf medium. Among these 16 N fixers, N fixing nifH, nifD and nifK genes was detected through PCR in 8, 7 and 1 strain respectively using gene specific primers designed in the study with Enterobacter sp. having all three (nifHKD) genes. Isolated bacteria showed resemblance to diverse genera such as Bacillus, Pseudomonas, Enterobacter, Citrobacter, Acinetobacter, Serratia, Klebsiella and Enterococcus based on 16S rRNA gene sequence analysis. In addition to showing the best mineral solubilization and metal resistance potential, Citrobacter sp. and Enterobacter sp. also removed 87%, 79% and 43% and 86%, 78% and 51% of Ni, Cd and Pb, respectively, from aqueous solution. These potent bacteria may be exploited both for bioremediation and biofertilization of wastewater irrigated soils leading to sustainable agriculture.

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Capsicum Frutescens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

Isolation and identification of plant growth-promoting bacteria associated with tall fescue

2009 ◽  
pp. 211-216 ◽  
Author(s):  
J. Monk ◽  
E. Gerard ◽  
S. Young ◽  
K. Widdup ◽  
M. O'Callaghan
Keyword(s):  
New Zealand ◽  
Plant Growth ◽  
Tall Fescue ◽  
Festuca Arundinacea ◽  
Plant Growth Promoting Bacteria ◽  
Beneficial Bacteria ◽  
Isolation And Identification ◽  
Naturally Occurring ◽  
Plant Growth Promoting ◽  
Growth Promoting

Tall fescue (Festuca arundinacea) is a useful alternative to ryegrass in New Zealand pasture but it is slow to establish. Naturally occurring beneficial bacteria in the rhizosphere can improve plant growth and health through a variety of direct and indirect mechanisms. Keywords: rhizosphere, endorhiza, auxin, siderophore, P-solubilisation

Sign in / Sign up

Export Citation Format

Share Document