Evaluation of the effects of Salvia officinalis essential oil on plasma biochemistry, gut mucus and quantity of acidic and neutral mucins in the chicken gut

Abstract In this study the effects of Salvia officinalis L. essential oil on the thickness of the gut mucus layer and quantity of neutral and acidic mucins in chickens were observed. Oneday-old chickens of Isa Brown breed were divided into five groups (n = 9) and fed 11 weeks as follows: control group (C): basal diet; experimental groups (E1 - E4) - same as C + sage essential oil (EO) in concentrations of 0.01%; 0.025%; 0.05% and 0.1%, respectively. The thickness of the mucus layer in the duodenum significantly increased in E3(0.05% sage EO) compared to E1 (0.01 % sage EO). In E3 (0.05% sage EO) the number of goblet cells containing acidic and neutral mucins was significantly decreased in the duodenum and jejunum and increased in the ileum compared to C. Feeding the diet supplemented with Se and 0.01% sage EO (E1) decreased plasma cholesterol level in comparison with E3(0.05 % sage EO). The addition of 0.05% (E3) and 0.1% sage EO (E4) to the diet caused a decrease in calcium plasma level compared to E2 (0.025 % sage EO). Plasma glucose level was significantly decreased in groups fed 0.05% (E3) and 0.025% sage EO (E2) compared with 0.01% sage (E1). The weight of internal organs was not affected by the diets. Our results suggest that effects of sage EO on the adherent mucus layer dynamics and mucin type distribution in the chicken intestine are dependent on sage EO dose and intestinal segment and there is still a need for further studies in order to obtain a plausible explanation.

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Effect of dietary Salvia officinalis essential oil and sodium selenite supplementation on antioxidative status and blood phagocytic activity in broiler chickens

Acta Veterinaria Brno ◽

10.2754/avb201382010043 ◽

2013 ◽

Vol 82 (1) ◽

pp. 43-48 ◽

Cited By ~ 10

Author(s):

Miroslav Ryzner ◽

Jana Takáčová ◽

Klaudia Čobanová ◽

Iveta Plachá ◽

Katarína Venglovská ◽

...

Keyword(s):

Essential Oil ◽

Phagocytic Activity ◽

Broiler Chickens ◽

Antioxidant Status ◽

Sodium Selenite ◽

Total Antioxidant Status ◽

Basal Diet ◽

Salvia Officinalis ◽

Control Group ◽

Antioxidative Status

Selected indicators of the antioxidant status and blood phagocytic activity were investigated in broiler chickens fed diets enriched with essential oil from Salvia officinalis and/or sodium selenite for 5 weeks. Twenty four one-day-old Ross 308 hybrid broilers were randomly divided into four groups according to dietary treatments. The control group was fed unsupplemented basal diet, whereas the feeds of three treatment groups were supplemented with essential oil (0.05%), sodium selenite (0.4 mg Se/kg dry matter) or their combination, respectively. The intake of diets containing essential oil alone or together with Se significantly elevated (P < 0.05) the total antioxidant status in plasma. The combination of essential oil and sodium selenite added to the basal diet resulted in significant reduction (P < 0.05) of superoxide dismutase activity in erythrocytes, increased activities of glutathione peroxidase in liver (P < 0.001) and duodenal mucosa (P < 0.05) compared to the control. Higher malondialdehyde concentrations in kidney tissues (P < 0.01) of chickens supplemented with sodium selenite were reduced by addition of essential oil to their diet. Blood phagocytic activity was significantly (P < 0.001) increased in both groups supplemented with selenium. The presented results demonstrate that Salvia officinalis essential oil and sodium selenite jointly added to diets had a more positive effect on the antioxidant status of broilers than when both additives were administered separately.

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Contribution to the Optimization of a Gas Chromatographic Method by QbD Approach used for Analysis of Essential Oils from Salvia officinalis

Revista de Chimie ◽

10.37358/rc.19.6.7266 ◽

2019 ◽

Vol 70 (6) ◽

pp. 2015-2020

Author(s):

Silvia Robu ◽

Aurelia Romila ◽

Olimpia Dumitriu Buzia ◽

Adrian Florin Spac ◽

Camelia Diaconu ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Plant Material ◽

Mobile Phase ◽

Chromatographic Method ◽

Salvia Officinalis ◽

Salvia Officinalis L ◽

Gas Chromatographic Method

Numerous articles on Salvia officinalis L. have been published regarding the composition of their essential oil. The considerable variation found may be due to the quality of the plant material as well as to the methods used for analysis. A simple GC-MS method was developed and optimized in the QbD approach, for the determination of sage essential oils. The optimization of GC-MS analysis was performed using different mobile phase flows, injection volumes, split ratios and temperature programs. The optimized method proved to be simple and can be successfully applied for the determination of sage essential oils.

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