scholarly journals Genotypic correlation between Salmonella Enteritidis isolates from broiler breeders and hatchery flocks

2021 ◽  
Vol 71 (4) ◽  
pp. 392-402
Author(s):  
İnci Başak Müştak ◽  
Hamit Kaan Müştak ◽  
Seyyide Sarıçam İnce
Keyword(s):  
Salmonella Enteritidis ◽  
Sequence Type ◽  
Farm Worker ◽  
Pfge Analysis ◽  
Broiler Breeder ◽  
Black Sea Region ◽  
Group I ◽  
Environmental Materials ◽  
Single Genotype ◽  
Cross Transmission

Abstract In this study, Salmonella Enteritidis strains isolated from dust and environmental materials from different flocks located in Turkey’s Western Black Sea region were examined by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). A total of 59 S. Enteritidis strains isolated from broiler breeder and hatchery flocks, and one S. Enteritidis strain isolated from a stool sample of a farm worker were examined. PFGE analysis revealed two major PFGE groups and nine different macro restriction profiles. It was determined that 85% (51/60) of the strains were close to each other and comprised Group I. All S. Enteritidis strains had the same sequence type (ST): ST11. Isolation of strains with a single genotype suggests that there may be a cross transmission between the flocks.

scholarly journals Mobile Colistin Resistance Gene mcr-1 Detected on an IncI2 Plasmid in Salmonella Typhimurium Sequence Type 19 from a Healthy Pig in South Korea

2021 ◽  
Vol 9 (2) ◽  
pp. 398
Author(s):  
Dong Chan Moon ◽  
Su-Jeong Kim ◽  
Abraham Fikru Mechesso ◽  
Hee Young Kang ◽  
Hyun-Ju Song ◽  
...  
Keyword(s):  
South Korea ◽  
Salmonella Typhimurium ◽  
Bacterial Infections ◽  
Salmonella Enteritidis ◽  
Sequence Type ◽  
Intestinal Epithelial ◽  
Colistin Resistance ◽  
Salmonella Gallinarum ◽  
Additional Resistance ◽  
Food Animals

Colistin is considered the last resort for the treatment of multi-drug resistant Gram-negative bacterial infections. We studied colistin resistance and the mcr-1 gene carriage in Salmonella isolates recovered from food animals in South Korea between 2010 and 2018. Colistin resistance was found in 277 isolates, predominantly in Salmonella Enteritidis (57.1%) and Salmonella Gallinarum (41.9%). However, the mcr-1 gene was identified in only one colistin-resistant Salmonella Typhimurium (MIC = 16 µg/mL) isolated from a healthy pig. The mcr-1 carrying isolate presented additional resistance to multiple antimicrobials. The strain belonged to sequence type (ST)19 and carried various virulence factor genes that are associated with adhesion and invasion of Salmonella into intestinal epithelial cells, as well as its survival in macrophages. The mcr-1 gene was identified on an IncI2 plasmid and it was also transferred to the E. coli J53 recipient strain. The mcr-1-carrying plasmid (pK18JST013) in this study was closely related to that previously reported in S. Indiana (pCFSA664-3) from chicken in China. This is the first report of mcr-1 carrying S. Typhimurium in South Korea. The finding indicates the importance of regular screening for the presence of the mcr-1 gene in S. Typhimurium in food animals to prevent the spread to humans.

Genome sequence of a clinical Salmonella Enteritidis sequence type 11 strain from South Africa

2019 ◽  
Vol 19 ◽  
pp. 164-166 ◽  
Author(s):  
Roxanne Rule ◽  
Mohamed Said ◽  
Nontombi Mbelle ◽  
John Osei Sekyere
Keyword(s):  
South Africa ◽  
Genome Sequence ◽  
Salmonella Enteritidis ◽  
Sequence Type

scholarly journals A common Salmonella Enteritidis sequence type from poultry and human gastroenteritis in Ibagué, Colombia

Biomédica ◽  
2019 ◽  
Vol 39 ◽  
pp. 50-62 ◽  
Author(s):  
Luz Clemencia Fandiño ◽  
Noel Verjan
Keyword(s):  
Multilocus Sequence Typing ◽  
Salmonella Enteritidis ◽  
Sequence Type ◽  
Salmonella Spp

Introducción. Salmonella Enteritidis es una de las mayores causas de salmonelosis en el mundo, siendo los huevos contaminados y la carne de pollo cruda sus principales fuentes de infección. En Ibagué, Colombia, se identificaron los principales serovares circulando en granjas, superficies de huevos y canales de pollo, sin embargo, se desconoce si esos serovares son responsables de gastroenteritis. Objetivo. Evaluar la relación genética entre aislamientos de Salmonella Enteritidis de aves de corral y humanos con gastroenteritis mediante multilocus sequence typing (MLST). Materiales y métodos. Se aisló Salmonella spp., de casos clínicos de gastroenteritis (n=110). Se realizó test de sensibilidad antibiótica, seguido de serotipificación y tipificación por medio de MLST y se comparó S. Enteritidis de humanos frente a S. Enteritidis de granjas ponedoras y de huevo comercializado (n=6). Resultados. Se aislaron 10 cepas de Salmonella spp., a partir de heces de humanos con gastroenteritis. Se obtuvo una prevalencia de Salmonella spp. de 9.09%, siendo S. Enteritidis (n=4), S. Typhymurium (n=2), S. Newport (n=1), S. Grupensis (n=1), S. Uganda (n=1) y S. Braenderup (n=1) los serotipos presentes en pacientes con gastroenteritis. El MLST indico que un tipo de secuencia común (ST11) de S. Enteritidis estuvo presente en todas las tres fuentes y mostraron el mismo patrón de resistencia antibiótica. Conclusión. S. Enteritidis ST11 constituye un vínculo entre el consumo/manipulación de huevos contaminados y gastroenteritis humana en Ibagué. Son necesarios estudios complementarios para conocer si otros serovares de Salmonella aislados de carne de pollo cruda también se asocian con la gastroenteritis humana.

Genetic Diversity of Cronobacter sakazakii Isolates Collected from a Swiss Infant Formula Production Facility

2013 ◽  
Vol 76 (5) ◽  
pp. 883-887 ◽  
Author(s):  
ANDREA MÜLLER ◽  
ROGER STEPHAN ◽  
CLAUDIA FRICKER-FEER ◽  
ANGELIKA LEHNER
Keyword(s):  
Infant Formula ◽  
Monitoring Program ◽  
Sequence Type ◽  
Powdered Infant Formula ◽  
Cronobacter Sakazakii ◽  
Production Facility ◽  
Pfge Analysis ◽  
O Antigen ◽  
Selection For ◽  
Prevalent Species

In this study, 141 Cronobacter isolates that were collected based on a hygienic monitoring program performed in a powdered infant formula production facility in Switzerland between September 2011 and October 2012 were further characterized. Isolates were identified to the species level by molecular methods, and strains of Cronobacter sakazakii were further subtyped by applying PCR-based O-antigen serotyping, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE). C. sakazakii was the most prevalent species identified (93.6%). Among this collection of isolates, representatives of all but one O-antigen serotype (serotype O5) were recognized. MLST analysis of 19 selected isolates revealed that most of the typeable isolates belonged to sequence type (ST) 4. Correlations between ST4 and serotype O2 and between ST83 and serotype O7 were observed. PFGE analysis revealed clusters with multiple isolates, including strains from samples collected at different time points and sampling sources. Generally, the observed heterogeneity among strains collected over the 13 months of the monitoring program was high, suggesting a constant flux among strains rather than a selection for persisting organisms.

Control, prevention and eradication of Salmonella enteritidis infection in broiler and broiler breeder flocks

1989 ◽  
Vol 125 (22) ◽  
pp. 545-548 ◽  
Author(s):  
S. McIlroy ◽  
R. McCracken ◽  
S. Neill ◽  
J. O'Brien
Keyword(s):  
Salmonella Enteritidis ◽  
Broiler Breeder

scholarly journals Intraspecies Genomic Groups in Enterococcus faecium and Their Correlation with Origin and Pathogenicity

2002 ◽  
Vol 68 (3) ◽  
pp. 1381-1391 ◽  
Author(s):  
Marc Vancanneyt ◽  
Angiolella Lombardi ◽  
Christian Andrighetto ◽  
Edo Knijff ◽  
Sandra Torriani ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Enterococcus Faecium ◽  
Fragment Length Polymorphism ◽  
Random Amplified Polymorphic Dna ◽  
Clinical Samples ◽  
Pfge Analysis ◽  
Group I ◽  
Dna Reassociation ◽  
Rapd Pcr ◽  
Vancomycin Resistant

ABSTRACT Seventy-eight Enterococcus faecium strains from various sources were characterized by random amplified polymorphic DNA (RAPD)-PCR, amplified fragment length polymorphism (AFLP), and pulsed-field gel electrophoresis (PFGE) analysis of SmaI restriction patterns. Two main genomic groups (I and II) were obtained in both RAPD-PCR and AFLP analyses. DNA-DNA hybridization values between representative strains of both groups demonstrated a mean DNA-DNA reassociation level of 71%. PFGE analysis revealed high genetic strain diversity within the two genomic groups. Only group I contained strains originating from human clinical samples or strains that were vancomycin-resistant or beta-hemolytic. No differentiating phenotypic features between groups I and II were found using the rapid ID 32 STREP system. The two groups could be further subdivided into, respectively, four and three subclusters in both RAPD-PCR and AFLP analyses, and a high correlation was seen between the subclusters generated by these two methods. Subclusters of group I were to some extent correlated with origin, pathogenicity, and bacteriocinogeny of the strains. Host specificity of E. faecium strains was not confirmed.

Effect of Vaccinating Breeder Chickens with a Killed Salmonella Vaccine on Salmonella Prevalences and Loads in Breeder and Broiler Chicken Flocks

2011 ◽  
Vol 74 (5) ◽  
pp. 727-734 ◽  
Author(s):  
R. D. BERGHAUS ◽  
S. G. THAYER ◽  
J. J. MAURER ◽  
C. L. HOFACRE
Keyword(s):  
Confidence Interval ◽  
Broiler Chicken ◽  
Salmonella Enteritidis ◽  
Most Probable Number ◽  
Sample Type ◽  
Broiler Breeder ◽  
Probable Number ◽  
Commercial Broiler ◽  
Antibody Titers ◽  
Matched Design

The objective of this study was to evaluate the effect of vaccination of breeder chickens on Salmonella prevalences and loads in breeder and broiler chicken flocks. Chickens housed on six commercial breeder farms were vaccinated with a killed Salmonella vaccine containing Salmonella Typhimurium, Salmonella Enteritidis, and Salmonella Kentucky. Unvaccinated breeders placed on six additional farms served as controls. Eggs from vaccinated and unvaccinated breeder flocks were kept separately in the hatchery, and the resulting chicks were used to populate 58 commercial broiler flock houses by using a pair-matched design. Vaccinated breeder flocks had significantly higher Salmonella-specific antibody titers than did the unvaccinated breeder flocks, although they did not differ significantly with respect to environmental Salmonella prevalences or loads. Broiler flocks that were the progeny of vaccinated breeders had significantly lower Salmonella prevalences and loads than broiler flocks that were the progeny of unvaccinated breeders. After adjusting for sample type and clustering at the farm level, the odds of detecting Salmonella in samples collected from broiler flocks originating from vaccinated breeders were 62% lower (odds ratio [95% confidence interval] = 0.38 [0.21, 0.68]) than in flocks from unvaccinated breeders. In addition, the mean load of culture-positive samples was lower in broilers from vaccinated breeders by 0.30 log most probable number per sample (95% confidence interval of −0.51, −0.09; P = 0.004), corresponding to a 50% decrease in Salmonella loads. In summary, vaccination of broiler breeder pullets increased humoral immunity in the breeders and reduced Salmonella prevalences and loads in their broiler progeny, but did not significantly decrease Salmonella in the breeder farm environment.

scholarly journals Commonality among Fluoroquinolone-Resistant Sequence Type ST131 ExtraintestinalEscherichia coliIsolates from Humans and Companion Animals in Australia

2011 ◽  
Vol 55 (8) ◽  
pp. 3782-3787 ◽  
Author(s):  
Joanne L. Platell ◽  
Rowland N. Cobbold ◽  
James R. Johnson ◽  
Anke Heisig ◽  
Peter Heisig ◽  
...  
Keyword(s):  
Host Species ◽  
Companion Animals ◽  
Virulence Gene ◽  
Multidrug Resistant ◽  
Companion Animal ◽  
Sequence Type ◽  
Fluoroquinolone Resistance ◽  
Resistance Mutations ◽  
Pfge Analysis ◽  
Animal Isolates

ABSTRACTEscherichia colisequence type 131 (ST131), an emergent multidrug-resistant extraintestinal pathogen, has spread epidemically among humans and was recently isolated from companion animals. To assess for human-companion animal commonality among ST131 isolates, 214 fluoroquinolone-resistant extraintestinalE. coliisolates (205 from humans, 9 from companion animals) from diagnostic laboratories in Australia, provisionally identified as ST131 by PCR, selectively underwent PCR-based O typing andblaCTX-M-15detection. A subset then underwent multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE) analysis, extended virulence genotyping, antimicrobial susceptibility testing, and fluoroquinolone resistance genotyping. All isolates were O25b positive, except for two O16 isolates and one O157 isolate, which (along with six O25b-positive isolates) were confirmed by MLST to be ST131. Only 12% of isolates (25 human, 1 canine) exhibitedblaCTX-M-15. PFGE analysis of 20 randomly selected human and all 9 companion animal isolates showed multiple instances of ≥94% profile similarity across host species; 12 isolates (6 human, 6 companion animal) represented pulsotype 968, the most prevalent ST131 pulsotype in North America (representing 23% of a large ST131 reference collection). Virulence gene and antimicrobial resistance profiles differed minimally, without host species specificity. The analyzed ST131 isolates also exhibited a conserved, host species-independent pattern of chromosomal fluoroquinolone resistance mutations. However, eight (89%) companion animal isolates, versus two (10%) human isolates, possessed the plasmid-borneqnrBgene (P< 0.001). This extensive across-species strain commonality, plus the similarities between Australian and non-Australian ST131 isolates, suggest that ST131 isolates are exchanged between humans and companion animals both within Australia and intercontinentally.

scholarly journals Strains of the Group I Lineage of Acidovorax citrulli, the Causal Agent of Bacterial Fruit Blotch of Cucurbitaceous Crops, are Predominant in Brazil

2016 ◽  
Vol 106 (12) ◽  
pp. 1486-1494 ◽  
Author(s):  
Gustavo M. Silva ◽  
Ricardo M. Souza ◽  
Lichun Yan ◽  
Rui S. Júnior ◽  
Flavio H. V. Medeiros ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Effect Of Temperature ◽  
Pfge Analysis ◽  
Bacterial Fruit Blotch ◽  
Acidovorax Citrulli ◽  
Group I ◽  
Pathogenicity Tests ◽  
Group Ii

Bacterial fruit blotch (BFB), caused by the seedborne bacterium Acidovorax citrulli, is an economically important threat to cucurbitaceous crops worldwide. Since the first report of BFB in Brazil in 1990, outbreaks have occurred sporadically on watermelon and, more frequently, on melon, resulting in significant yield losses. At present, the genetic diversity and the population structure of A. citrulli strains in Brazil remain unclear. A collection of 74 A. citrulli strains isolated from naturally infected tissues of different cucurbit hosts in Brazil between 2000 and 2014 and 18 A. citrulli reference strains from other countries were compared by pulsed-field gel electrophoresis (PFGE), multilocus sequence analysis (MLSA) of housekeeping and virulence-associated genes, and pathogenicity tests on seedlings of different cucurbit species. The Brazilian population comprised predominantly group I strains (98%), regardless of the year of isolation, geographical region, or host. Whole-genome restriction digestion and PFGE analysis revealed that three unique and previously unreported A. citrulli haplotypes (assigned as haplotypes B22, B23, and B24) occurred in Brazil. The greatest diversity of A. citrulli (four haplotypes) was found among strains collected from the northeastern region of Brazil, which accounts for more than 90% of the country’s melon production. MLSA clearly distinguished A. citrulli strains into two well-supported clades, in agreement with observations based on PFGE analysis. Five Brazilian A. citrulli strains, representing different group I haplotypes, were moderately aggressive on watermelon seedlings compared with four group II strains that were highly aggressive. In contrast, no significant differences in BFB severity were observed between group I and II A. citrulli strains on melon and squash seedlings. Finally, we observed a differential effect of temperature on in vitro growth of representative group I and II A. citrulli haplotypes. Specifically, of 18 group II strains tested, all grew at 40 and 41°C, whereas only 3 of 15 group I strains (haplotypes B8[P], B3[K], and B15) grew at 40°C. Three strains representing haplotype B8(P) were the only group I strains that grew at 41°C. These results contribute to a better understanding of the genetic diversity of A. citrulli associated with BFB outbreaks in Brazil, and reinforce the efficiency of MLSA and PFGE analysis for assessing population structure. This study also provides the first evidence to suggest that temperature might be a driver in the ecological adaptation of A. citrulli populations.

Sign in / Sign up

Export Citation Format

Share Document