scholarly journals Genotyping of the prevalent Chlamydia trachomatis strains involved in cervical infections in women in Ahvaz, Iran

2010 ◽  
Vol 59 (9) ◽  
pp. 1023-1028 ◽  
Author(s):  
Behrouz Taheri Beni ◽  
Hossein Motamedi ◽  
Mohammad Roayaei Ardakani
Keyword(s):  
Chlamydia Trachomatis ◽  
Clinical Symptoms ◽  
Age Groups ◽  
Lower Abdominal Pain ◽  
Rflp Analysis ◽  
The Other ◽  
Pcr Rflp ◽  
First Time ◽  
Genotype F ◽  
Genotype E

To determine the prevalence of cervical Chlamydia trachomatis genotypes in Iran for the first time and their association with three clinical symptoms/signs, i.e. abnormal vaginal discharge, lower abdominal pain (LAP) and swab-induced bleeding, and patient age, 620 cervical specimens were obtained from women with symptomatic genital infection referred to gynaecological clinics and 108 C. trachomatis-positive specimens were genotyped by direct omp1 gene PCR-RFLP analysis. Eight genotypes were identified. The most prevalent genotype was E (31.5 %), followed by F (23.1 %), D/Da (13 %), K (9.2 %), I (8.3 %), G (7.5 %), H (5.5 %) and J (1.9 %). For analysing the association of C. trachomatis genotypes with symptoms/signs and age, P-values were separately evaluated for genogroups and genotypes. The analysis of genogroups showed that women infected with genogroup F/G manifested the symptom of LAP significantly more often than those infected with the other genogroups (P=0.02), while the analysis of genotypes revealed that women infected with genotype F reported LAP slightly more often than women infected with the other genotypes (P=0.08). No significant correlation between genogroups and age was found; however, genotype E was somewhat less prevalent among women aged 25–34 years than among other age groups (P=0.08).

scholarly journals Characterisation of avian Pasteurella multocida strains with PCR-RFLP analysis of the ompH gene

2013 ◽  
Vol 61 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Boglárka Sellyei ◽  
Éva Ivanics ◽  
Tibor Magyar
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Pasteurella Multocida ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
The Other ◽  
Pcr Rflp ◽  
H Gene ◽  
Geographic Regions ◽  
Type Strains ◽  
Restriction Fragment Length

The 16 somatic serotype type strains and 60 field isolates of Pasteurella multocida, representing various avian species and geographic regions in Hungary, were characterised by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the ompH gene with DraI restriction endonuclease. The type strains yielded eight different (I-VIII) profiles. Strains whose PCR fragment was uncut by DraI (profile IV) could be differentiated with HindIII and PvuII restriction endonucleases. Five of the eight PCR-RFLP profiles (I, III, V, VI and VII) were detected among the field strains. Only a correlation of limited strength was found between the classical somatic serotypes and the PCR-RFLP profiles. However, the results confirmed that molecular methods could confidently distinguish serotype A:1 strains from the other serotypes. Moreover, the specific relationship between somatic serotypes and PCR-RFLP types among isolates from turkey raises the possibility of the existence of host-specific clones within the P. multocida population.

scholarly journals Estrus Behaviour and Reproductive Traits of Pulawska Gilts Associated with Selected Gene Polymorphisms

2021 ◽  
Vol 21 (1) ◽  
pp. 159-172
Author(s):  
Marek Babicz ◽  
Marcin Pastwa ◽  
Magdalena Szyndler-Nędza ◽  
Anna Kozubska-Sobocińska ◽  
Barbara Danielak-Czech ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Gene Polymorphisms ◽  
Rflp Analysis ◽  
Reproductive Traits ◽  
The Other ◽  
Molecular Analyses ◽  
Native Breed ◽  
Hair Roots ◽  
Pcr Rflp ◽  
Pcr Conditions

Abstract Searching for the associations between the gene polymorphism and the reproductive traits is essential in defining the genetic native breed specificity, which distinguishes them from the other breeds. The aim of our study was to determine the associations between mutations in the PRL, PRLR, PTGS2, FUT1 genes and sexual and periparturient activity in native Pulawska gilts. The analysis included 72 animals which gave birth to the first litter. Evaluation of the productive value of gilts accounted for indicators of sexual and periparturient activity as well as reproductive traits. The biological material for molecular analyses was obtained from the hair roots of the gilts. The genotype was verified by PCR RFLP analysis. The primers and PCR conditions were determined on the basis of available literature data. Statistically significant differences (P≤0.05) were found at the PRL locus: gilts of AA genotypes (Ins/Ins) at the PRL locus were characterised by longest farrowing duration compared to gilts of AB genotype (P≤0.05). The analysis of PRLR gene showed that gilts of TT genotype revealed a tendency for later occurrence of estrus signs (first and second estrus) and for the markedly longest farrowings (P≤0.05). With regard to PTGS2 and FUT1 loci, no significant differences were found in the parameters of sexual and periparturient activity of the gilts. However, gilts of FUT1 GG genotype gave birth to and reared the largest first litters (P≤0.05). The results of the studies expand the knowledge about the genetic structure and productivity of Pulawska gilts.

scholarly journals Abiotrophia elegans Strains Comprise 8% of the Nutritionally Variant Streptococci Isolated from the Human Mouth

1999 ◽  
Vol 37 (8) ◽  
pp. 2553-2556 ◽  
Author(s):  
Setsuko Sato ◽  
Taisei Kanamoto ◽  
Masakazu Inoue
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Rflp Analysis ◽  
The Other ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Biochemical Tests ◽  
Pcr Rflp ◽  
Adh Activity

Ninety-one isolates of nutritionally variant streptococci (NVS) that were previously isolated from the human mouth were regarded as consisting of 7 Streptococcus defectivus isolates, 78Streptococcus adjacens isolates, and 6 Gemella morbillorum isolates. However, recent references to the taxonomic reclassification of NVS, from S. defectivusto Abiotrophia defectiva and from S. adjacensto Abiotrophia adiacens, and the newly introduced speciesAbiotrophia elegans as a third Abiotrophiaspecies, emphasize the need for genetic analyses for identification of NVS. When PCR-restriction fragment length polymorphism (RFLP) and phylogenetic distances were examined based on 16S rRNA gene sequences, the results indicated that 7 of the 91 NVS isolates were closely related to A. elegans. These seven isolates consisted of four isolates previously identified as G. morbillorum and three isolates previously identified as S. adjacens. Two isolates previously identified as G. morbillorum were related to A. adiacens. In biochemical tests, A. elegans and the seven isolates related to it possessed arginine dihydrolase (ADH) activity but the other Abiotrophia species did not. As a result, A. elegans strains comprised 8% of the 91 NVS isolates. Our findings suggest that A. elegans, A. adiacens, and A. defectiva exist in the human mouth in proportions of about 1:11:1 and that A. elegans can be genetically distinguished from the other twoAbiotrophia species by PCR-RFLP analysis of 16S rRNA gene sequences and can be biochemically distinguished by ADH activity.

Genetic polymorphisms of cytochrome P450 enzymes: CYP2C9, CYP2C19, CYP2D6, CYP3A4, and CYP3A5 in the Croatian population

2017 ◽  
Vol 32 (1) ◽  
Author(s):  
Lana Ganoci ◽  
Tamara Božina ◽  
Nikica Mirošević Skvrce ◽  
Mila Lovrić ◽  
Petar Mas ◽  
...  
Keyword(s):  
Rflp Analysis ◽  
The Other ◽  
Published Data ◽  
Cytochrome P450 Enzymes ◽  
Chain Reaction ◽  
Allelic Variants ◽  
Mediterranean Populations ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
European Populations

AbstractBackground:Data on the frequency of pharmacogenetic polymorphisms in the Croatian population are limited. We determined and analyzed frequencies for the most importantMethods:2637 subjects were included. Genotyping was performed by real-time polymerase chain reaction (PCR) using TaqMan® DME or TaqMan® SNP Genotyping Assays, and by PCR, and PCR-RFLP analysis.Results:ForConclusions:The frequency of the CYP allelic variants, genotypes, and predicted phenotypes in the Croatian population is in accordance with the other European populations, between the values of published data for Middle European and Mediterranean populations.

Molecular epidemiology ofBartonellaspecies isolated from ground squirrels and other rodents in northern California

2016 ◽  
Vol 144 (9) ◽  
pp. 1837-1844 ◽  
Author(s):  
A. C. ZIEDINS ◽  
B. B. CHOMEL ◽  
R. W. KASTEN ◽  
A. M. KJEMTRUP ◽  
C.-C. CHANG
Keyword(s):  
Rflp Analysis ◽  
Ground Squirrels ◽  
The Other ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Northern California ◽  
Wild Rodents ◽  
Neotoma Fuscipes ◽  
Pcr Rflp ◽  
California Ground Squirrels

SUMMARYBartonellaspp. are endemic in wild rodents in many parts of the world. A study conducted in two northern California counties (Sonoma and Yolo) sampling California ground squirrels (Otospermophilus beecheyi) and four other rodent species (Peromyscus maniculatus, P. boylii, P. trueiandNeotoma fuscipes) led to the isolation of small Gram-negative bacilli which were identified asBartonellaspp. based on colony morphology, polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and partial gene sequencing. Overall,Bartonellaspp. were isolated from the blood of 71% (32/45) of the ground squirrels and one third (22/66) of the other rodents. PCR–RFLP analysis of thegltA and 16S rRNA genes yielded seven unique profiles, four for the ground squirrels and three for the other rodents. Isolates from each PCR–RFLP profiles were submitted for partial sequencing. Ground squirrel isolates were most closely related toB. washoensis, whereas the other rodent isolates were closest toB. vinsoniisubsp.vinsoniiandB. vinsoniisubsp.arupensis. Two of these three species or subspecies are known zoonotic agents.

scholarly journals On the Helminth Fauna of the Muskrat (Ondatra zibethicus (Linnaeus, 1766)) in the Barnim District of Brandenburg State/Germany

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2444
Author(s):  
Rolf K. Schuster ◽  
Peter Specht ◽  
Siegfried Rieger
Keyword(s):  
Flood Control ◽  
Age Groups ◽  
The Other ◽  
Intermediate Hosts ◽  
Opisthorchis Felineus ◽  
Larval Stages ◽  
Burrowing Activity ◽  
Fur Animals ◽  
First Time ◽  
Plagiorchis Elegans

The muskrat is a neozoon species that has occupied many countries of continental North Europe after its introduction from north America as fur animals. Due to its burrowing activity it damages river and canal banks and structures of flood control. For this reason, the eradication of this alien species is recommended. Muskrats are also of parasitological interest since they can act as suitable intermediate hosts for Echinococcus multilocularis. On the other hand, little is known on the other helminths that infect muskrats. A total of 130 muskrats of different age groups trapped in different habitats in the Barnim district of the Brandenburg state by a professional hunter were examined for parasites and seven trematodes (Echinostoma sp., Notocotylus noyeri, Plagiorchis elegans, Plagiorchis arvicolae, Psilosostoma simillimum, P. spiculigerum, Opisthorchis felineus and four larval cestode species (Hydatigera taeniaeformis, Taenia martis, Taenia polyacantha, Taenia crassiceps) were detected. Larval stages of E. multilocularis were not found. O. felineus was found for the first time in muskrats in Germany. All the named parasites were present in Europe prior to the introduction of muskrats. With a prevalence of 48.9%, Strobilocercus fasciolaris, the larval stage of the cat tapeworm, H. taeniaeformis, was the most frequent parasite found in adult muskrats.

scholarly journals Analysis of ACE, AGTR1, eNOS, MTHFR, MTRR and APOE genes polymorphisms in the population of North-West of Russia

2004 ◽  
Vol 2 (3) ◽  
pp. 32-35
Author(s):  
Oleg S Glotov ◽  
Andrey S Glotov ◽  
Olga A Tarasenko ◽  
Tatyana E Ivashchenko ◽  
Vladislav S Baranov
Keyword(s):  
High Frequency ◽  
Middle Age ◽  
Low Frequency ◽  
Rflp Analysis ◽  
The Other ◽  
North West ◽  
Multifactorial Diseases ◽  
Pcr Rflp ◽  
Obvious Similarity ◽  
European Populations

Estimation of population-specific frequencies of relevant polymorphisms in normal control population remains the major prerequisite of these studies. The polymorphisms of seven genes (ACE, AGTR1, eNOS, MTHFR, MTRR, APOE) associated with some multifactorial diseases (cardiovascular diseases, diabetes) were studied by PCR-RFLP analysis in North-West population of Russia. The group consisted of unrelated individuals of middle age (25-45) (59 men and 58 women). Distribution of relevant polymorphisms frequencies for MTHFR, MTRR, APOE, eNOS and AGTR1 genes was similar in our samples compared to these ones in some and European populations (Spain and Germany). Low frequency of D/D genotype and high frequency of I/I genotype were found in Russian compared to Spain population (D/D - 29,6 and 41%, I/I - 26,1 and 15%, respectively). No significant statistical differences in distribution of ACE polymorphisms were found between Russian and Scotland populations (D/D - 31%, I/I - 20%). This population specific differences for the polymorphisms of some genes and its obvious similarity for the other ones were disclosed

scholarly journals Genetic Heterogeneity ofAlicyclobacillusStrains Revealed by RFLP Analysis ofvdcRegion andrpoBGene

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Agnieszka Dekowska ◽  
Jolanta Niezgoda ◽  
Barbara Sokołowska
Keyword(s):  
Cluster Analysis ◽  
16S Rdna ◽  
Genetic Heterogeneity ◽  
Reference Strain ◽  
Rflp Analysis ◽  
The Other ◽  
16S Rdna Gene ◽  
Restriction Patterns ◽  
Pcr Rflp ◽  
Reference Strains

PCR-RFLP targeting of the 16S rDNA andrpoBgenes, as well as thevdcregion, was applied to identify and differentiate between the spoilage and non-spoilageAlicyclobacillusspecies. Eight reference strains and 75 strains isolated from spoiled juices, juice concentrates, drinks, its intermediates, and fresh apples were subject to study. Hin6I restriction patterns of the 16S rDNA gene enabled distinguishing between all the species analyzed, while therpoBgene andvdcgene cluster analysis also revealed that there were two major types among theA. acidoterrestrisisolates, one similar to the reference strainA. acidoterrestrisDSM 2498, and the other similar to the reference strainA. acidoterrestrisATCC 49025. Heterogeneity was also observed among theA. acidocaldariusisolates. RFLP analysis of the 16S rDNA andrpoBgenes, as well asvdcregion, can be used successfully in the identification and research of intraspecies heterogeneity of theAlicyclobacillusspecies.

scholarly journals Detection of Stolbur phytoplasma on blackberry: A new natural host in Serbia

Genetika ◽  
2011 ◽  
Vol 43 (3) ◽  
pp. 559-568 ◽  
Author(s):  
Slobodan Kuzmanovic ◽  
Mira Starovic ◽  
Snezana Pavlovic ◽  
Veljko Gavrilovic ◽  
Goran Aleksic ◽  
...  
Keyword(s):  
Elongation Factor ◽  
Rflp Analysis ◽  
Late Summer ◽  
Type Ii ◽  
Rdna Genes ◽  
The Czech Republic ◽  
Tuf Gene ◽  
Pcr Rflp ◽  
Stolbur Phytoplasma ◽  
First Time

During the late summer of 2007, a severe phytoplasma-like disease was observed for the first time in blackberry plants (Rubus fruticosus), commercial cv. Cacanska beztrna. Redness and downward rolling of leaves were symptoms observed in three localities in Central Serbia. The presence of Stolbur phytoplasma, belonging to the taxonomic subgroup 16SrXII-A, in diseased samples was confirmed by the PCR - RFLP analysis of 16S rDNA genes and elongation factor Tu (tuf) gene. A sequence analysis of the tuf gene confirmed homology with phytoplasmas stolbur tuf-type II detected previously in Italian grapevines and red clovers in the Czech Republic. This is the first report of Stolbur phytoplasma 16SrXII-A group tuf-type II on blackberries in Serbia.

scholarly journals COVID-19 lockdown in Spain: Psychological impact is greatest on younger and vulnerable children

2021 ◽  
pp. 135910452110550
Author(s):  
Josep Matalí-Costa ◽  
Ester Camprodon-Rosanas
Keyword(s):  
Psychological Functioning ◽  
Social Impact ◽  
Clinical Symptoms ◽  
Psychological Symptoms ◽  
Psychological Impact ◽  
Age Groups ◽  
Online Questionnaire ◽  
Vulnerable Children ◽  
Increased Risk ◽  
First Time

In 2020, quarantine home confinement measures were implemented in Spain to control a pandemic for the first time. The objective of this study is to assess both the psychological and social impact of that ‘lockdown’ on different age groups of children and adolescents and also the different strategies adopted by families in that situation. We collected data via an online questionnaire answered by 850 participants, parents of young people aged between 4 and 18, between 24th April and 8th May 2020. Our results show that living in a home smaller than 80 m2 is associated with an increased risk of presenting clinical symptoms of emotional or behavioural distress, with an odds ratio of 2.54 (95% CI: 1.07–6.02), p = 0.034; while increased age is a predictor of better psychological functioning during lockdown: coefficient of 0.25 (95% CI: 0.07–0.41), p = 0.004. Moreover, age is also a marker of higher scores on the scale of psychological symptoms, with a coefficient of 0.02 (95% CI: 0.01–0.04), p = .005. To conclude, younger children present poorer behavioural and emotional functioning and more symptoms of emotional distress than adolescents during lockdown. Living in a small home is a risk factor for presenting clinical symptoms. These findings should be taken into consideration when planning for future home confinement episodes and when considering preventative strategies in the realm of mental health care.

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