scholarly journals Association between ATP citrate lyase (ACLY) gene polymorphism and fattening, slaughter and pork quality traits in polish pigs

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Arkadiusz Terman ◽  
Katarzyna Woźniak-Męch ◽  
Agnieszka Korpal ◽  
Daniel Polasik ◽  
Mirosław Tyra ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Average Daily Gain ◽  
Pork Quality ◽  
Quality Traits ◽  
Atp Citrate Lyase ◽  
Large White ◽  
Citrate Lyase ◽  
Texture Parameters ◽  
Pcr Rflp ◽  
Negative Effect

Abstract The primary aim of this study was to estimate the relationship between ATP citrate lyase (ACLY) gene polymorphism (c.*523 T>C) and fattening and pork quality traits. Investigations were carried out on 526 pigs represented by three breeds: Polish Landrace (n=269), Polish Large White (n=189) and Puławska (n=68). ACLY genotypes were determined by PCR–RFLP method. It was demonstrated that the analyzed polymorphism had significant influence (p< 0.05 and p ≤ 0.01) on several economically important traits in pigs e.g. average daily gain, average backfat thickness, lean meat percentage. The results obtained allow for application of c.*523 T>C polymorphism in breeding programs to improve the pig population in terms of fattening and slaughter traits. However, this breeding program may have a slight negative effect on meat texture parameters.

scholarly journals Analysis of FTO and PLIN2 Polymorphisms in Relation to Carcass and Meat Quality Traits in Pigs

2019 ◽  
Vol 19 (1) ◽  
pp. 71-83 ◽  
Author(s):  
Daniel Polasik ◽  
Eva-Maria Kamionka ◽  
Mirosław Tyra ◽  
Grzegorz Żak ◽  
Arkadiusz Terman
Keyword(s):  
Meat Quality ◽  
Fat Content ◽  
Longissimus Dorsi ◽  
Quality Traits ◽  
Large White ◽  
Meat Quality Traits ◽  
Pcr Rflp ◽  
Perilipin 2 ◽  
Water Holding ◽  
Intramuscular Fat Content

AbstractThe aim of this study was to analyze the association of polymorphisms in alpha-ketoglutarate-dependent dioxygenase (FTO) and perilipin 2 (PLIN2) genes with carcass and meat quality traits in pigs reared in Poland. The research covered 578 sows that belong to the following breeds: Duroc, Hampshire, Polish Landrace, Pietrain, Puławska and Polish Large White. FTO (FM244720:g.400C>G) and PLIN2 (GU461317:g.98G>A) genes variants were determined by means of PCR-RFLP and ACRS-PCR methods respectively. Association between individual genotypes and analyzed traits was calculated by means of GLM procedure for Polish Landrace, Polish Large White and Puławska breeds separately and for all six breeds together in case of FTO gene. The results showed that FTO variants were associated with weight of loin without backfat and skin (WL), loin eye area (AL) and meat percentage (MP) in Polish Large White (P≤0.05), mean backfat thickness from 5 measurements (BFT) and pH measured 45 min after slaughter in m. longissimus dorsi (pH24 ld) as well as with water holding-capacity (WHC) in Puławska breed (P≤0.01). PLIN2 genotypes, however were correlated with WL and height of the loin eye (HL) in Polish Large White and Puławska (P≤0.05), AL in Polish Large White (P≤0.01) as well as luminosity (L*) in Puławska (P≤0.05) pigs. We observed most consistent relationships of PLIN2 SNP with intramuscular fat content (IMF) and WHC. In 3 analyzed breeds GG genotype was connected with highest values of these traits (P≤0.05).

scholarly journals Effects of BF Gene Polymorphism on the Reproductive Performance of Hybrid Polish Landrace X Polish Large White Sows

2016 ◽  
Vol 66 (1) ◽  
pp. 125-131
Author(s):  
Dorota Napierała ◽  
Maria Kawęcka ◽  
Eugenia Jacyno ◽  
Beata Matysiak ◽  
Anita Kołodziej-Skalska
Keyword(s):  
Gene Polymorphism ◽  
Litter Size ◽  
Reproductive Performance ◽  
Reproductive Traits ◽  
Specific Primers ◽  
Large White ◽  
Potential Relationship ◽  
Pcr Rflp

AbstractThe aim of this study was to determine the potential relationship between variants of the BF gene and litter size in Polish Landrace x Polish Large White sows. To identify polymorphism within the BF gene, the PCR-RFLP method was applied, using specific primers and the SmaI enzyme. The researchers identified the presence of two alleles, T and C, with frequencies: 0.68 and 0.32, giving three genotypes with frequencies: 0.49, 0.37 and 0.14, respectively for TT, TC and CC. Analysis of relationships between the various genotypes of the BF gene and selected reproductive traits showed significant differences. Sows with TT genotype in the BF locus, gave birth to significantly more live piglets in the first litter (10.24) compared with sows with the CC genotype (7.13).

scholarly journals Effects of immunocastration on growth performance, body composition, meat quality, and boar taint

2017 ◽  
Vol 62 (No. 6) ◽  
pp. 249-258
Author(s):  
R. Stupka ◽  
J. Čítek ◽  
K. Vehovský ◽  
K. Zadinová ◽  
M. Okrouhlá ◽  
...  
Keyword(s):  
Growth Performance ◽  
Meat Quality ◽  
Average Daily Gain ◽  
Carcass Composition ◽  
The Other ◽  
Boar Taint ◽  
Large White ◽  
Study Objective ◽  
Negative Effect ◽  
Daily Gain

The study objective was to evaluate the effect of immunocastration in the period between the first and second vaccinations and subsequently between the second vaccination and slaughter on growth performance, carcass composition, meat quality, and boar taint, and compare results in immunocastrated males (IC), uncastrated boars (UCM), surgically castrated barrows (CM), and gilts (FE). The study included 70 pigs of the Duroc × (Large White × Landrace) crossbreed. Upon the overall assessment of the selected fattening indicators (average daily gain, feed intake), significant differences between CM and the other groups were demonstrated. Meanwhile, no significant differences were found between the IC, UCM, and FE groups. In this test, immunocastrates showed no negative effect from the second vaccination in relation to those carcass value indicators evaluated in comparison with UCM and FE. CM showed adversely lower carcass value parameters compared the other groups. No significant differences in pH, meat colour, drip loss, shear force, and intramuscular fat were found. The values of these indicators obtained for IC converged with those measured in UCM and FE. It was demonstrated that immunocastration prevented the occurrence of undesired boar taint. Androstenone decreased by 77% and skatole by 71% in IC as compared to UCM.

scholarly journals Association analysis of seven candidate genes with performance traits in Czech Large White pigs

2011 ◽  
Vol 56 (No. 8) ◽  
pp. 337-344 ◽  
Author(s):  
F. Weisz ◽  
T. Urban ◽  
P. Chalupová ◽  
A. Knoll
Keyword(s):  
Average Daily Gain ◽  
Large White ◽  
Association Analyses ◽  
Lean Meat ◽  
Meat Content ◽  
Pcr Rflp ◽  
Performance Traits ◽  
Estimated Breeding Values ◽  
Study Association ◽  
Daily Gain

&nbsp;In this study association analyses were performed between genes tagged SNP (IGF2, NAMPT, DGAT1, MYF4, MC3R, MC4R and MYOD1), performance traits (backfat thickness, lean meat content, average daily gain from birth to the end of the test, average daily gain in test) and estimated breeding values (EBVs) in a population of Czech Large White sows (n = 101). Genotyping of all SNPs was performed by SNapShot with the exception of SNP within NAMPT gene for which HpaII PCR-RFLP assay was used. The following significant associations between genes tagged SNPs and traits or EBVs were found out: DGAT1 &ndash; lean meat content (AG &gt; AA, P &le; 0.05), MC4R &ndash; EBV for lean meat content (GG &gt; AA, P &le; 0.05; GG &gt; AG, P &le; 0.05), IGF2 &ndash; EBV for reproduction (piglets born alive in the second and subsequent parity) (AG &gt; AA, P &le; 0.05) and total EBV (AG &gt; AA, P &le; 0.01) and MC3R &ndash; EBV for average daily gain (CT &gt; TT, P &le; 0.05).

scholarly journals Feedlot performance, carcass composition and pork quality from entire male and female Landrace and Large White market-weight pigs

1991 ◽  
Vol 71 (1) ◽  
pp. 29-42 ◽  
Author(s):  
A. P. Sather ◽  
S. D. M. Jones ◽  
S. Joyal
Keyword(s):  
Meat Quality ◽  
Average Daily Gain ◽  
Live Weight ◽  
Carcass Composition ◽  
Quality Traits ◽  
Feed Conversion ◽  
Feedlot Performance ◽  
Improvement Program ◽  
Large White ◽  
Meat Quality Traits

A total of 80 pigs, representing Large White and Landrace boars and gilts were slaughtered at an average live weight of 92.3 kg (80.5 – 102.5 kg). Boars were housed in groups of five pigs per pen, and were individually fed to appetite twice daily for a period of 45 min. Gilts were housed in groups of 12 pigs per pen. The diet consisted of 21% crude protein with 3300 kcal kg−1 digestible energy. Carcasses were weighed on the slaughter floor and cooled at 1 °C for 24 h prior to dissection of the left side into fat, bone and lean. There were no significant breed-by-gender interactions for growth, carcass and meat quality traits. These pigs had low average backfat, when measured ultrasonically according to Canadian Swine Improvement Program standards on the live pig (gilts 12.3 mm, boars 10.1 mm), high average daily gain (ADG: gilts 880, boars 940 g d−1) and low age adjusted to 90 kg (gilts 148, boars 145 d). Large White boars had a lower feed conversion ratio (2.18 vs. 2.32, P = 0.015) and less feed intake per day (2.05 vs. 2.14 kg d−1P = 0.020) than did Landrace boars. There were no significant differences in dissected lean yield between breeds (P = 0.389) or genders (P = 0.148). However, gilts had greater carcass grade fat and greater lean depth measurements than did boars, such that both genders had a predicted lean yield of 51.8%. Gilts (40.8 cm2) had a larger (P < 0.001) longissimus dorsi area than boars (38.0 cm2). There were no breed effects on the lean content of the four major cuts (P = 0.20), but boars had less fat (P = 0.01) and more bone than gilts (P < 0.01). There were few consistent differences in subjective or objective meat quality traits (i.e. color, drip loss) among breeds or genders. Boars had muscle with greater moisture than gilts (P < 0.001) as well as softer fat (P = 0.016). Thus, it appears possible to produce pork of high quality from lean animals and that lean boars efficiently produce carcasses of equivalent yield and quality to carcasses produced by lean gilts. Key words: Swine, boar, carcass composition, meat quality, Landrace, Large White

scholarly journals Genetic variation of loin and ham quality in Finnish Landrace and Large White pigs

2008 ◽  
Vol 16 (2) ◽  
pp. 89 ◽  
Author(s):  
M-L. SEVÓN-AIMONEN ◽  
M. HONKAVAARA ◽  
T. SERENIUS
Keyword(s):  
Meat Quality ◽  
Fixed Effects ◽  
Average Daily Gain ◽  
Genetic Correlations ◽  
Genetic Effect ◽  
Biceps Femoris ◽  
Additive Genetic Effect ◽  
Drip Loss ◽  
Quality Traits ◽  
Large White

Selection potential for meat quality of economically important loin (longissimus) and ham muscles (adductor, semimembranosus, biceps femoris) has been assessed. Ultimate pH (pHu), meat colour (lightness, redness and yellowness), drip loss and two visually scored colour traits were recorded from 483 Finnish Landrace and 494 Finnish Large White station test pigs in a half-sib design. A univariate restricted maximum likelihood procedure was used to estimate variance components. The statistical model contained age at beginning of test, sex and time lapse from slaughter to dissection as fixed effects and slaughter batch, common environment of littermates and additive genetic effect of the animal as random effects. The average pHu values in adductor and semispinalis were between 5.6 and 6.1. The pHu were on average 5.4 and 5.5 in longissimus and semimembranosus respectively, with the latter two being lower than optimum values of 5.6 to 5.9. Lightness for semimembranosus turned to be clearly lighter (62) than for other muscles. Lightness for longissimus (56) was slightly lighter than optimum (from 48 to 54). The heritability varied from zero to 0.45 for pHu, from 0.02 to 0.34 for lightness, from 0.17 to 0.56 for redness, from zero to 0.28 for yellowness and from 0.05 to 0.16 for drip loss. Heritability for redness values was considerably higher than heritability for other meat quality traits. The heritability of quality traits spoke for possibilities for genetic improvement of meat quality. Genetic correlations between quality traits (pHu and lightness) and average daily gain varied strongly among breeds and muscles. Genetic correlations between meat-% and pHu were in most cases high and unfavourable (rg from –0.36 to –0.68 except in longissimus, where it was 0.11). Genetic correlations between meat-% and lightness were unfavourable in Finnish Large White (from 0.47 to 0.92) but in Finnish Landrace estimates varied among muscles (from –0.40 to 0.47). Due to these results, the ham quality (pHu and lightness for semimembranosus) was included in the selection criteria for pork quality in the Finnish pig improvement programme.;

scholarly journals Association analysis of interleukin-18 gene with performance traits in Czech Large White pigs

2012 ◽  
Vol 60 (5) ◽  
pp. 97-102 ◽  
Author(s):  
Pavla Chalupová ◽  
T. Urban ◽  
A. Knoll
Keyword(s):  
Average Daily Gain ◽  
Backfat Thickness ◽  
Breeding Value ◽  
Interleukin 18 ◽  
Large White ◽  
Lean Meat ◽  
Meat Content ◽  
Pcr Rflp ◽  
Performance Traits ◽  
Daily Gain

This research focused on recently described MspI and VspI SNPs in interleukin-18 gene and their association with selected performance traits (backfat thickness; lean meat content, average daily gain from birth, average daily gain in test, breeding value for average daily gain, for lean meat content, for reproduction and total breeding value) in a population of 344 Czech Large White sows. Both SNPs were genotyped by PCR-RFLP. In this work, these polymorphisms were associated with backfat thickness and lean meat content. Animals with genotype AA (MspI SNP) had significantly (P < 0.05) lower backfat thickness and higher lean meat content (0.77 ± 0.02 and 62.16 ± 0.23, resp.) compared to GA (0.83 ± 0.02 and 61.40 ± 0.18, resp.). Animals with genotype AA (VspI SNP) had significantly (P < 0.05) lower lean meat content and higher backfat thickness (61.10 ± 0.36 and 0.85 ± 0.03, resp.) compared to GA (62.02 ± 0.16 and 0.77 ± 0.02, resp.). No association between IL-18 polymorphisms and other performance traits was found. Our study revealed that IL-18 could be candidate gene for backfat thickness and lean meat content in pigs.

scholarly journals Relationship between VRTN gene polymorphism and growth, slaughter and meat quality traits in three polish pig breeds

2018 ◽  
Vol 42 (5) ◽  
pp. 540-549
Author(s):  
Daniel Polasik ◽  
Mirosław Tyra ◽  
Magdalena Szyndler-Nędza ◽  
Agnieszka Korpal ◽  
Katarzyna Woźniak-Męch ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Meat Quality ◽  
Growth Traits ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Quality Traits ◽  
Carcass Yield ◽  
Texture Parameters ◽  
Carcass Parameters ◽  
Intramuscular Fat Content

ABSTRACT Earlier studies have shown that insertion/deletion polymorphism (g.20311_20312ins291) in vertnin (VRTN) gene is associated with number of vertebrate in pigs. Because vertebral number determines the size of important meat cuts and influences carcass conformation, the aim of this study was to estimate the relationship between VRTN gene polymorphism and growth, slaughter and pork quality traits including texture parameters. Investigations were carried out on 526 pigs represented three breeds: Polish Landrace, Polish Large White and Puławska. VRTN gene polymorphism was determined by PCR methodology. Association study was performed for each breed separately. It was demonstrated that the analyzed polymorphism had significant influence on several economically important porcine traits. Among growth traits VRTN genotypes were associated with test and lifetime daily gains, number of days on test (p≤0.05) and age of slaughter (p≤0.01, p≤0.05). For carcass parameters, relationships were noticed for carcass yield (p≤0.01), weight of loin and its area, average backfat thickness and weight of primary cuts (p≤0.05). Taking into consideration meat quality, VRTN polymorphism influenced intramuscular fat content, meat colour (yellowness) and pH24 (p≤0.05). For texture parameters, it was correlated with toughness, cohesiveness, chewiness (p≤0.01, p≤0.05) and firmness (p≤0.05) measured in M. longissimus dorsi and M. semimembranosus. Only intramuscular fat content, chewiness of ham, carcass yield and cohesiveness of loin showed unequivocal tendency. In case of the first trait, each breed Wt/Wt genotype was associated with its highest values (p≤0.05), while the second and third traits, Q/Q genotype was correlated with its lowest values (p≤0.01, p≤0.05). Highest loin cohesiveness was observed in animals with Wt/Q genotype (p≤0.01, p≤0.05).

scholarly journals Effect of the oestrogen receptor (ESR) gene on reproductive traits of Large White, White Meaty and Landrace pigs

2011 ◽  
Vol 50 (No. 6) ◽  
pp. 249-253 ◽  
Author(s):  
R. Omelka ◽  
M. Bauerová ◽  
J. Mlynek ◽  
B. Buchová ◽  
D. Peškovičová ◽  
...  
Keyword(s):  
Oestrogen Receptor ◽  
Reproductive Traits ◽  
Positive Association ◽  
Large White ◽  
Pcr Rflp ◽  
Negative Effect ◽  
Breeding Farms ◽  
Esr Gene

We investigated the effect of oestrogen receptor (ESR) gene on total number of born (TNB), number of born alive (NBA) and number of weaned (NW) piglets in Large White (LW), White Meaty (WM) and Landrace (L) sows from six Slovak breeding farms. Detection of ESR (Pvu II) genotypes was performed by the PCR-RFLP method. The frequencies of favourable B allele in LW, WM and L were 0.33, 0.25 and 0.08, respectively. We found a highly significant effect of ESR locus on NW (P &le; 0.01) in LW, however, we observed a negative effect of BB genotype on the trait. In WM, a positive association of B allele with TNB, NBA and NW was found but the differences were not confirmed statistically. A highly significant effect of ESR locus on TNB, NBA and NW (P &le; 0.01) was identified only in L breed. We found an increase of +0.62 &plusmn; 0.18 (TNB), +0.65 &plusmn; 0.18 (NBA) and +0.51 &plusmn; 0.16 (NW) pigs per copy of B allele. &nbsp;

CAPN1 gene as a potential marker for growth performance and carcass characteristics in pigs

2017 ◽  
Vol 57 (6) ◽  
pp. 1014
Author(s):  
K. Ropka-Molik ◽  
E. Robert ◽  
M. Tyra ◽  
K. Piórkowska ◽  
M. Oczkowicz ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Meat Quality ◽  
Carcass Characteristics ◽  
Pork Quality ◽  
Quality Traits ◽  
Nucleotide Polymorphism ◽  
Present Stage ◽  
Large White ◽  
Single Nucleotide ◽  
Potential Marker

The calcium-activated neutral proteinase 1 (CAPN1) gene, which encodes µ-calpain, is considered to be associated with the majority of meat-quality traits. The objective of the present study was to evaluate the effect of CAPN1 polymorphism on the most important slaughter, fattening and pork-quality traits. The genotype frequency of g.1429G>A polymorphism was estimated on 860 pigs represented by five breeds (Polish Landrace, Polish Large White, Pietrain, Duroc and Pulawska). In all breeds, the most frequent were pigs with an AA genotype (60–74%), with the exception of Duroc pigs where the largest number of GG animals was observed (14%). The results showed that g.1429G>A polymorphism significantly affected the number of days on test (P ≤ 0.05) and age at slaughter (P ≤ 0.01), the weight of ham and primary cuts (P ≤ 0.05, P ≤ 0.01 respectively), loin eye area (P ≤ 0.01) and average backfat thickness (P ≤ 0.05). The g.1429G>A single-nucleotide polymorphism showed no clear influence on complex pork-quality traits. At the present stage of research, the results obtained allow for the use of g.1429G>A polymorphism in CAPN1 gene as genetic marker in pig selection to improve several slaughter or fattening traits.

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