scholarly journals Association analysis of interleukin-18 gene with performance traits in Czech Large White pigs

Author(s):  
Pavla Chalupová ◽  
T. Urban ◽  
A. Knoll

This research focused on recently described MspI and VspI SNPs in interleukin-18 gene and their association with selected performance traits (backfat thickness; lean meat content, average daily gain from birth, average daily gain in test, breeding value for average daily gain, for lean meat content, for reproduction and total breeding value) in a population of 344 Czech Large White sows. Both SNPs were genotyped by PCR-RFLP. In this work, these polymorphisms were associated with backfat thickness and lean meat content. Animals with genotype AA (MspI SNP) had significantly (P < 0.05) lower backfat thickness and higher lean meat content (0.77 ± 0.02 and 62.16 ± 0.23, resp.) compared to GA (0.83 ± 0.02 and 61.40 ± 0.18, resp.). Animals with genotype AA (VspI SNP) had significantly (P < 0.05) lower lean meat content and higher backfat thickness (61.10 ± 0.36 and 0.85 ± 0.03, resp.) compared to GA (62.02 ± 0.16 and 0.77 ± 0.02, resp.). No association between IL-18 polymorphisms and other performance traits was found. Our study revealed that IL-18 could be candidate gene for backfat thickness and lean meat content in pigs.

2011 ◽  
Vol 56 (No. 8) ◽  
pp. 337-344 ◽  
Author(s):  
F. Weisz ◽  
T. Urban ◽  
P. Chalupová ◽  
A. Knoll

&nbsp;In this study association analyses were performed between genes tagged SNP (IGF2, NAMPT, DGAT1, MYF4, MC3R, MC4R and MYOD1), performance traits (backfat thickness, lean meat content, average daily gain from birth to the end of the test, average daily gain in test) and estimated breeding values (EBVs) in a population of Czech Large White sows (n = 101). Genotyping of all SNPs was performed by SNapShot with the exception of SNP within NAMPT gene for which HpaII PCR-RFLP assay was used. The following significant associations between genes tagged SNPs and traits or EBVs were found out: DGAT1 &ndash; lean meat content (AG &gt; AA, P &le; 0.05), MC4R &ndash; EBV for lean meat content (GG &gt; AA, P &le; 0.05; GG &gt; AG, P &le; 0.05), IGF2 &ndash; EBV for reproduction (piglets born alive in the second and subsequent parity) (AG &gt; AA, P &le; 0.05) and total EBV (AG &gt; AA, P &le; 0.01) and MC3R &ndash; EBV for average daily gain (CT &gt; TT, P &le; 0.05).


2009 ◽  
Vol 54 (No. 10) ◽  
pp. 443-447 ◽  
Author(s):  
J. Zrůstová ◽  
A. Knoll ◽  
T. Urban ◽  
S. Čepica

Visfatin encoded by the <I>NAMPT</I> gene <I>(nicotinamide phosphoribosyltransferase,</I> formerly also known as <I>PBEF – pre-B cell colony-enhancing factor</I>) is suggested to play a role in lipid metabolism and pathophysiology of diabetes mellitus type 2. A new microsatellite <I>SCZ004</I> was detected within intron 9 of the <I>NAMPT</I> gene. In Czech Large White (<I>n</I> = 95) frequencies of alleles <I>282, 286, 287, 299</I>, and <I>304</I> were 0.02, 0.39, 0.07, 0.04, and 0.48, respectively. Allele <I>286</I> was predominant also in Landrace (<I>n</I> = 11) and Black Pied Prestice (<I>n</I> = 11) breeds. Association analysis was carried out between previously reported SNP AM999341:g.669T>C in intron 9 of the <I>NAMPT</I> gene and backfat thickness, average daily gain and lean meat content in Czech Large White (<I>n</I> = 215), Black Pied Prestice (<I>n</I> = 96) and Landrace (<I>n</I> = 105). The <I>CC</I> genotype was associated with higher backfat thickness (<I ≤ 0.01) in Black Pied Prestice, however in Czech Large White <I>CC</I> was associated with lower backfat thickness when compared to <I>TT</I> and <I>CT</I> genotypes (<I ≤ 0.05). In Czech Large White, <I>CC</I> genotype was associated with higher lean meat content when compared to <I>CT</I> (not significant) and <I>TT</I> genotype (<I ≤ 0.05) while in Landrace <I>CC</I> had the lowest lean meat content when compared to <I>CT</I> and <I>TT</I> genotypes but only the difference between <I>CC</I> and <I>CT</I>T was statistically significant (<I ≤ 0.05). In Black Pied Prestice no association with lean meat content was found. Average daily gain was not associated with the SNP in any breed.


2006 ◽  
Vol 82 (4) ◽  
pp. 455-462 ◽  
Author(s):  
J. Wolf ◽  
D. Peškovičová ◽  
E. Žáková ◽  
E. Groeneveld

AbstractThe data sets consisted of field performance data from 54 848 purebred and 16 175 crossbred animals (Czech data set, CZ) and 16 610 purebred and 9 228 crossbred animals (Slovak data set, SK). Animals from the following breeds were included: Duroc, Hampshire, Piétrain, sire line of Large White (CZ) or Yorkshire (SK), Czech Meat pig (CZ) or Slovak Meat pig (SK), Belgian Landrace (SK). Two-trait animal models were calculated for average daily gain from birth to the end of the field test (ADG) and lean meat content (LM, only in CZ) or backfat thickness (BF, only in SK). The models included additive breed and breed heterotic effects. Piétrain was the breed with the highest LM and the lowest BF. The additive genetic breed effect was about 1·5% LM (CZ) in comparison with Large White or −0·4 mm BF (SK) in comparison to Yorkshire. The sire line of Large White (CZ) or the Yorkshire breed (SK) clearly exceeded all the remaining sire breeds in ADG (on average by 30 to 50 g/day). There was a clear tendency to negative heterosis in LM in all crossbred combinations (CZ). In BF, heterotic effects between −0·2 mm and +0·3 mm were estimated, mostly not being significant (SK). The estimates of the heterotic effects for ADG were positive throughout. Higher values up to 40 g/day (7%) were observed in the Czech data set. Though heterotic breed effects are of some importance especially for ADG, their inclusion in the equations for breeding value estimation will have only a minor impact on the predicted breeding values.


Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 331
Author(s):  
Pavel Nevrkla ◽  
Eva Václavková ◽  
Miroslav Rozkot

The study was designed for the comparison of barrows and gilts of two contrasting genotypes in terms of growth ability, carcass value and meat quality. A total of 76 pigs (from 7 ± 1 kg to 95 ± 6 kg) of the indigenous breed Prestice Black-Pied (PB) and the commercial hybrid of Large White × Landrace × Large Whitesireline (CH) were included in this study. The groups were balanced regarding genotype and sex. Live weight at the age of 79 days (p < 0.001), live weight at slaughter (p < 0.01), average daily gain (p < 0.001), weight of carcass and left carcass side (p < 0.05) and ham proportion (p < 0.001) were higher in the CH group. The values of the given parameters were higher in barrows than in gilts. The effect of genotype and sex interaction was observed in lean meat content (p < 0.05) and backfat thickness (p < 0.01). The CH gilts showed the highest lean meat content and the lowest backfat thickness compared to the lowest values in the PB barrows. The value of pH45 (p < 0.01) and intramuscular fat content were higher in the PB group. The lightness (p < 0.001), yellowness (p < 0.01), drip loss (p < 0.05) and protein content were lower in the PB group. Higher values of lightness (p < 0.05), yellowness (p < 0.05) and cholesterol content (p < 0.05) were observed in the boars. Oxidative stability of the meat (malondialdehyde content) was more favorable in the PB pigs. The effect of genotype and sex interaction was confirmed for the pH24 value (the highest value was found in the PB barrows and the lowest in the CH barrows, p < 0.001) and for the dry matter content (lowest in the PB gilts and highest in the CH barrows, p < 0.05). Overall, the PB pigs were characterized by inferior growth and carcass parameters, however, with more favorable parameters for meat quality.


2004 ◽  
Vol 78 (1) ◽  
pp. 61-66 ◽  
Author(s):  
A. T. Kanengoni ◽  
K. Dzama ◽  
M. Chimonyo ◽  
J. Kusina ◽  
S. M. Maswaure

AbstractA study was conducted to compare the performance of Mukota, Large White (LW) and LW ✕ Mukota F1pigs given increasing levels of maize cob meal. Sixteen female weaners of each genotype were given, ad libitum, diets containing 0, 100, 200 and 300 g maize cob meal per kg of diet for 14 weeks. The diets were designed to contain similar levels of protein (ca. 160 g crude protein per kg) and energy (ca. 9 MJ metabolizable energy per kg). Average daily food intake per unit metabolic body weight (ADFI per kg M0·75), average daily gain (ADG) and food conversion ratio (FCR) were determined. The pigs were slaughtered and cold dressed mass (CDM) and backfat thickness were determined for each pig. The ADFI per kg M0·75was similar among the four diets for the three genotypes. The rate of decline in ADG was higher (P < 0·05) in the LW than in the other two genotypes. The FCR in the Mukota was poorer (P < 0·05) than that in the LW and the F1crosses. The Mukota had the highest (P < 0·05) backfat thickness (K5 and K7·5) values of the three genotypes across the four diets, followed by the F1crosses. The CDM values for the LW and the F1crosses were similar and were higher (P < 0·05) than those for the Mukota. The findings indicate that F1crosses and the Mukota were better able to utilize diets containing high levels of maize cob meal than LW pigs.


2009 ◽  
Vol 54 (No. 8) ◽  
pp. 359-364 ◽  
Author(s):  
Z. Vykoukalová ◽  
A. Knoll ◽  
S. Čepica

Perilipin, encoded by the <I>PLIN</I> gene, is a lipid droplet-associated phosphoprotein that functions as a key regulator of triacylglycerol storage and hydrolysis in adipocytes. In this study, structure and variability of the porcine <I>PLIN</I> gene were characterised. PCR fragments encompassing exons 1 to 9 and interspersed introns were sequenced and the obtained sequence was deposited in EMBL/GenBank/DDBJ databases (AM931171). Seven silent polymorphisms and one nonsynonymous polymorphism were detected in the coding sequence. The nonsynonymous polymorphism g.627G>A causing an amino acid substitution p.Val3Ile was found only in Meishan and Meishan × Piétrain cross. Associations were studied between loci g.4119A>G and g.7966T>C, and average daily gain (ADG), backfat thickness (BFT) or lean meat content (LMC) in 166 gilts from two herds. Due to supposed complete linkage disequilibrium between loci g.4119A>G and g.7966T>C only genotype combinations <I>AA-TT, AG-TC</I> and <I>GG-CC</I> were detected. A significant difference (<I>P</I> = 0.0290) between <I>GG-CC</I> and <I>AG-TC</I> genotype combinations for BFT in herd 2 and a suggestive difference (<I>P</I> = 0.0516) between <I>GG-CC</I> and <I>AA-TT</I> genotypes for ADG in herd 1 were detected.


2009 ◽  
Vol 78 (2) ◽  
pp. 267-272 ◽  
Author(s):  
Dorota Stępień-Poleszak ◽  
Arkadiusz Pietruszka ◽  
Maria Kawęcka

The study was aimed at defining leptin gene polymorphism and its potential association with values of particular features of fattening and slaughter value of gilts of Line 990. The study included a total of 208 gilts. The polymorphic locus in LEP gene was identified by the restriction enzyme HinfI in 3469 position, by using the polymerase chain reaction-restriction fragment-length polymorphism (PCR–RFLP) method. Two alleles of LEP gene were identified: T (0.94) and C (0.06), resulting in two genotypes: TC (0.12) and TT (0.88). We did not observe any gilts of CC genotype. The analysis of values of fattening and slaughter features, depending on LEP genotype did not reveal significant differences in body mass increase, daily gain from day 63 to 180, and daily gain from birth to day 180, feed conversion per 1 kg body mass and the loin-eye thickness. Significant differences between the LEP genotypes were present for such features as the backfat thickness at points P2 (p ⪬ 0.05) and P4 (p ⪬ 0.01) and average backfat thickness (p ⪬ 0.01) in favour of TT genotype. We noted higher average values of lean meat content in carcass in favour of TT homozygotes, compared to the heterozygotes (p ⪬ 0.05). The investigation contributes additonal information regarding LEP gene polymorphism in gilts of Line 990. Knowledge of LEP genotypes may be useful to improve the slaughter value in gilts primarily due to less fatness. Due to the lack of individuals representing CC genotype in our results, research should continue on a larger population.


2020 ◽  
Vol 60 ◽  
pp. 5-11
Author(s):  
I. V. Verbuch ◽  
H. V. Bratkovska

Goal. To accelerate the selection process and create populations of pigs with high level of productivity to assess the most effective modern methods breeding value of large white pigs in the SE "DG" Pasichna "Institute of Feed and Agriculture Podillya NAAS" Starosinyavsky district of Khmelnytsky region on the basis of a combined assessment of repair young for their own productivity and assessment meat qualities by MD Berezovsky and B. Tyler and the most promising method BLUP. Methods. Comparison, zootechnical and biometric analyzes. Results. The breeding value of pigs of large white breed of the breeding farm of SE "Pasichna" ICSGP NAAS "Starosinyavsky district of Khmelnytsky region by modern effective methods, namely: BLUP. According to the average indicators of the combined assessment of own productivity of animals at control fattening, taking into account their live weight on the date of birth and weaning, average daily gain, live weight and age of 100 kg, body length and fat thickness, boars significantly outweighed pigs. Compared with pigs, their live weight at the date of birth and weaning (at the age of 30 days) was higher by 0.1 and 0.81 kg. The age of reaching a live weight of 100 kg exceeded by 5.43 days, the average daily gain of 0.016 kg. Live weight and body length of pigs on the date of fat thickness measurement was greater by 4.04 kg and 1.03 cm. Lifetime fat thickness at three measurement points at the level of 6–7 thoracic vertebrae, on the buttocks and in the midpoint of the back between withers and buttocks was thinner by 0.92; 0.94 and 1.06 mm. Evaluation indices of fattening and meat qualities by growth energy and fat thickness (Ia) and average daily gain and fat thickness (Iv) in boars indicate their higher level by 2.07 and 7.67 points compared to pigs and are equal to, respectively, 95.12 ± 1.95 and 152.96 ± 2.38 points. The coefficient of variability (Cv, %) of indicators of own productivity and estimated indices of fattening and meat qualities of repair boars ranged from 1.92% (body length on the date of fat thickness measurement, cm) to 15.12% (age of live weight 100 kg, days), repair pigs – from 4.63% (body length on the date of fat thickness measurement, cm) to 28.19% (index of assessment for growth energy and fat thickness, (Ia), points). The best average values of indicators of breeding value of animals are found on the basis of modern, most promising method BLUP, because it is considered in terms of mathematics, statistics and selection is the most accurate, well-founded, has the best theoretical basis and the lowest error variance. The average value of the BLUP index (parent lines) for boars of large white breed is 101.71 ± 1.681, pigs – 96.94 ± 0.724 points. At the same time, boars outweighed pigs by 4.77 points. The most positive correlation between live weight of piglets at weaning with their body length (r = 0.16), average daily gain (r = 0.19) and fat thickness (r = 0.24), the average correlation between the indices of fattening and meat qualities according to MD Berezovsky and B. Tyler (r = 0.56) P > 0.99 and the weak correlation between live weight of piglets at weaning and the BLUR index (r = 0,21) P > 0.99. It should be noted that there are almost no correlations between the fattening and meat quality indices of pigs and the BLUP index. Conclusions. In the conditions of breeding and specialized farms for the production of commercial pork in the region, to determine the breeding value of animals for further reproduction of their own herd and sale to other breeding farms of Ukraine, to systematically assess repair young animals for their own productivity and clear qualities of pigs and the most perfect BLUP index.


2018 ◽  
Vol 58 (10) ◽  
pp. 1915
Author(s):  
Damian Knecht ◽  
Anna Jankowska-Mąkosa ◽  
Sebastian Środoń ◽  
Kamil Duziński

The aim of the present study was to demonstrate the influence of housing and feeding systems on selected fattening and slaughter parameters of finishers with different genotypes. The experimental population consisted of 1200 finishers in three genetic variants from the Landrace (L), Large White (LW), Duroc (D) and Pietrain (P) breeds: 400 finishers L × [D × P] genotype, 400 finishers LW × [D × P] genotype and 400 finishers [L × LW] × [D × P] genotype. Subgroups were isolated for each genetic variant with the experimental factors: housing system (slatted floor or deep litter) and feeding system (dry or liquid). Selected fattening parameters were analysed: final liveweight (kg), mortality (%), average daily gain (g/day) and feed conversion ratio (kg/kg gain). Additionally, slaughter parameters were analysed: carcass weight (kg), height of longissimus dorsi (LD) muscle (mm), backfat thickness (mm) and lean meat content (%). The housing system strongly affected the final liveweight, average daily gain and carcass weight. To a lesser degree, this factor determined the mortality, feed conversion ratio, height of LD muscle, backfat thickness and lean meat content. The feeding system substantially affected almost all fattening parameters, except for the mortality. Statistical analysis of slaughter parameters showed that the impact of the feeding system was confirmed statistically only in terms of carcass weight. Genotype largely determined the final liveweight, average daily gain, carcass weight and lean meat content. Taking into account interactions of all factors, the most favourable effect in terms of production was to fattening four-way crossbreeds [L × LW] × [D × P] on slatted floors and fed by liquid feeding.


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