scholarly journals Association analysis of seven candidate genes with performance traits in Czech Large White pigs

2011 ◽  
Vol 56 (No. 8) ◽  
pp. 337-344 ◽  
Author(s):  
F. Weisz ◽  
T. Urban ◽  
P. Chalupová ◽  
A. Knoll

 In this study association analyses were performed between genes tagged SNP (IGF2, NAMPT, DGAT1, MYF4, MC3R, MC4R and MYOD1), performance traits (backfat thickness, lean meat content, average daily gain from birth to the end of the test, average daily gain in test) and estimated breeding values (EBVs) in a population of Czech Large White sows (n = 101). Genotyping of all SNPs was performed by SNapShot with the exception of SNP within NAMPT gene for which HpaII PCR-RFLP assay was used. The following significant associations between genes tagged SNPs and traits or EBVs were found out: DGAT1 – lean meat content (AG > AA, P ≤ 0.05), MC4R – EBV for lean meat content (GG > AA, P ≤ 0.05; GG > AG, P ≤ 0.05), IGF2 – EBV for reproduction (piglets born alive in the second and subsequent parity) (AG > AA, P ≤ 0.05) and total EBV (AG > AA, P ≤ 0.01) and MC3R – EBV for average daily gain (CT > TT, P ≤ 0.05).

Author(s):  
Pavla Chalupová ◽  
T. Urban ◽  
A. Knoll

This research focused on recently described MspI and VspI SNPs in interleukin-18 gene and their association with selected performance traits (backfat thickness; lean meat content, average daily gain from birth, average daily gain in test, breeding value for average daily gain, for lean meat content, for reproduction and total breeding value) in a population of 344 Czech Large White sows. Both SNPs were genotyped by PCR-RFLP. In this work, these polymorphisms were associated with backfat thickness and lean meat content. Animals with genotype AA (MspI SNP) had significantly (P < 0.05) lower backfat thickness and higher lean meat content (0.77 ± 0.02 and 62.16 ± 0.23, resp.) compared to GA (0.83 ± 0.02 and 61.40 ± 0.18, resp.). Animals with genotype AA (VspI SNP) had significantly (P < 0.05) lower lean meat content and higher backfat thickness (61.10 ± 0.36 and 0.85 ± 0.03, resp.) compared to GA (62.02 ± 0.16 and 0.77 ± 0.02, resp.). No association between IL-18 polymorphisms and other performance traits was found. Our study revealed that IL-18 could be candidate gene for backfat thickness and lean meat content in pigs.


2009 ◽  
Vol 54 (No. 10) ◽  
pp. 443-447 ◽  
Author(s):  
J. Zrůstová ◽  
A. Knoll ◽  
T. Urban ◽  
S. Čepica

Visfatin encoded by the <I>NAMPT</I> gene <I>(nicotinamide phosphoribosyltransferase,</I> formerly also known as <I>PBEF – pre-B cell colony-enhancing factor</I>) is suggested to play a role in lipid metabolism and pathophysiology of diabetes mellitus type 2. A new microsatellite <I>SCZ004</I> was detected within intron 9 of the <I>NAMPT</I> gene. In Czech Large White (<I>n</I> = 95) frequencies of alleles <I>282, 286, 287, 299</I>, and <I>304</I> were 0.02, 0.39, 0.07, 0.04, and 0.48, respectively. Allele <I>286</I> was predominant also in Landrace (<I>n</I> = 11) and Black Pied Prestice (<I>n</I> = 11) breeds. Association analysis was carried out between previously reported SNP AM999341:g.669T>C in intron 9 of the <I>NAMPT</I> gene and backfat thickness, average daily gain and lean meat content in Czech Large White (<I>n</I> = 215), Black Pied Prestice (<I>n</I> = 96) and Landrace (<I>n</I> = 105). The <I>CC</I> genotype was associated with higher backfat thickness (<I ≤ 0.01) in Black Pied Prestice, however in Czech Large White <I>CC</I> was associated with lower backfat thickness when compared to <I>TT</I> and <I>CT</I> genotypes (<I ≤ 0.05). In Czech Large White, <I>CC</I> genotype was associated with higher lean meat content when compared to <I>CT</I> (not significant) and <I>TT</I> genotype (<I ≤ 0.05) while in Landrace <I>CC</I> had the lowest lean meat content when compared to <I>CT</I> and <I>TT</I> genotypes but only the difference between <I>CC</I> and <I>CT</I>T was statistically significant (<I ≤ 0.05). In Black Pied Prestice no association with lean meat content was found. Average daily gain was not associated with the SNP in any breed.


2009 ◽  
Vol 54 (No. 8) ◽  
pp. 359-364 ◽  
Author(s):  
Z. Vykoukalová ◽  
A. Knoll ◽  
S. Čepica

Perilipin, encoded by the <I>PLIN</I> gene, is a lipid droplet-associated phosphoprotein that functions as a key regulator of triacylglycerol storage and hydrolysis in adipocytes. In this study, structure and variability of the porcine <I>PLIN</I> gene were characterised. PCR fragments encompassing exons 1 to 9 and interspersed introns were sequenced and the obtained sequence was deposited in EMBL/GenBank/DDBJ databases (AM931171). Seven silent polymorphisms and one nonsynonymous polymorphism were detected in the coding sequence. The nonsynonymous polymorphism g.627G>A causing an amino acid substitution p.Val3Ile was found only in Meishan and Meishan × Piétrain cross. Associations were studied between loci g.4119A>G and g.7966T>C, and average daily gain (ADG), backfat thickness (BFT) or lean meat content (LMC) in 166 gilts from two herds. Due to supposed complete linkage disequilibrium between loci g.4119A>G and g.7966T>C only genotype combinations <I>AA-TT, AG-TC</I> and <I>GG-CC</I> were detected. A significant difference (<I>P</I> = 0.0290) between <I>GG-CC</I> and <I>AG-TC</I> genotype combinations for BFT in herd 2 and a suggestive difference (<I>P</I> = 0.0516) between <I>GG-CC</I> and <I>AA-TT</I> genotypes for ADG in herd 1 were detected.


2006 ◽  
Vol 82 (4) ◽  
pp. 455-462 ◽  
Author(s):  
J. Wolf ◽  
D. Peškovičová ◽  
E. Žáková ◽  
E. Groeneveld

AbstractThe data sets consisted of field performance data from 54 848 purebred and 16 175 crossbred animals (Czech data set, CZ) and 16 610 purebred and 9 228 crossbred animals (Slovak data set, SK). Animals from the following breeds were included: Duroc, Hampshire, Piétrain, sire line of Large White (CZ) or Yorkshire (SK), Czech Meat pig (CZ) or Slovak Meat pig (SK), Belgian Landrace (SK). Two-trait animal models were calculated for average daily gain from birth to the end of the field test (ADG) and lean meat content (LM, only in CZ) or backfat thickness (BF, only in SK). The models included additive breed and breed heterotic effects. Piétrain was the breed with the highest LM and the lowest BF. The additive genetic breed effect was about 1·5% LM (CZ) in comparison with Large White or −0·4 mm BF (SK) in comparison to Yorkshire. The sire line of Large White (CZ) or the Yorkshire breed (SK) clearly exceeded all the remaining sire breeds in ADG (on average by 30 to 50 g/day). There was a clear tendency to negative heterosis in LM in all crossbred combinations (CZ). In BF, heterotic effects between −0·2 mm and +0·3 mm were estimated, mostly not being significant (SK). The estimates of the heterotic effects for ADG were positive throughout. Higher values up to 40 g/day (7%) were observed in the Czech data set. Though heterotic breed effects are of some importance especially for ADG, their inclusion in the equations for breeding value estimation will have only a minor impact on the predicted breeding values.


2018 ◽  
Vol 58 (10) ◽  
pp. 1915
Author(s):  
Damian Knecht ◽  
Anna Jankowska-Mąkosa ◽  
Sebastian Środoń ◽  
Kamil Duziński

The aim of the present study was to demonstrate the influence of housing and feeding systems on selected fattening and slaughter parameters of finishers with different genotypes. The experimental population consisted of 1200 finishers in three genetic variants from the Landrace (L), Large White (LW), Duroc (D) and Pietrain (P) breeds: 400 finishers L × [D × P] genotype, 400 finishers LW × [D × P] genotype and 400 finishers [L × LW] × [D × P] genotype. Subgroups were isolated for each genetic variant with the experimental factors: housing system (slatted floor or deep litter) and feeding system (dry or liquid). Selected fattening parameters were analysed: final liveweight (kg), mortality (%), average daily gain (g/day) and feed conversion ratio (kg/kg gain). Additionally, slaughter parameters were analysed: carcass weight (kg), height of longissimus dorsi (LD) muscle (mm), backfat thickness (mm) and lean meat content (%). The housing system strongly affected the final liveweight, average daily gain and carcass weight. To a lesser degree, this factor determined the mortality, feed conversion ratio, height of LD muscle, backfat thickness and lean meat content. The feeding system substantially affected almost all fattening parameters, except for the mortality. Statistical analysis of slaughter parameters showed that the impact of the feeding system was confirmed statistically only in terms of carcass weight. Genotype largely determined the final liveweight, average daily gain, carcass weight and lean meat content. Taking into account interactions of all factors, the most favourable effect in terms of production was to fattening four-way crossbreeds [L × LW] × [D × P] on slatted floors and fed by liquid feeding.


2013 ◽  
Vol 58 (No. 9) ◽  
pp. 429-436 ◽  
Author(s):  
E. Krupa ◽  
J. Wolf

Genetic parameters for total number of piglets born per litter, number of piglets weaned per litter, lean meat content, and average daily gain from birth till the end of the field test were estimated for Czech Large White (445 589 records) and Czech Landrace (149 057 records) pigs using a four-trait animal model. The following heritabilities were estimated (first number: Large White, second number: Landrace): 0.10 &plusmn; 0.004 and 0.09 &plusmn; 0.007 for total number born; 0.09 &plusmn; 0.005 and 0.07 &plusmn; 0.008 for number weaned; 0.39 &plusmn; 0.004 and 0.36 &plusmn; 0.009 for lean meat content; 0.21 &plusmn; 0.004 and 0.18 &plusmn; 0.006 for daily gain. The highest genetic correlation (approximately 0.85 in both breeds) was estimated between both litter size traits. In Czech Landrace, all remaining genetic correlations were &lt; 0.20 in their absolute value. Negative correlations of approximately ‑0.25 were estimated in Czech Large White between daily gain and both reproduction traits. All remaining correlations in Czech Large White were also &lt; 0.20 in their absolute value. The estimated non-zero correlations between production and reproduction traits are, besides of other arguments, one reason to recommend a joint genetic evaluation of production and reproduction traits. If more than one litter trait is included in the genetic evaluation, repeatability models should be used instead of separate treating the first and the second and subsequent litters; this is because of the high correlations among litter size traits which are expected to cause numerical problems if multi-parity models are used. &nbsp;


Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 331
Author(s):  
Pavel Nevrkla ◽  
Eva Václavková ◽  
Miroslav Rozkot

The study was designed for the comparison of barrows and gilts of two contrasting genotypes in terms of growth ability, carcass value and meat quality. A total of 76 pigs (from 7 ± 1 kg to 95 ± 6 kg) of the indigenous breed Prestice Black-Pied (PB) and the commercial hybrid of Large White × Landrace × Large Whitesireline (CH) were included in this study. The groups were balanced regarding genotype and sex. Live weight at the age of 79 days (p < 0.001), live weight at slaughter (p < 0.01), average daily gain (p < 0.001), weight of carcass and left carcass side (p < 0.05) and ham proportion (p < 0.001) were higher in the CH group. The values of the given parameters were higher in barrows than in gilts. The effect of genotype and sex interaction was observed in lean meat content (p < 0.05) and backfat thickness (p < 0.01). The CH gilts showed the highest lean meat content and the lowest backfat thickness compared to the lowest values in the PB barrows. The value of pH45 (p < 0.01) and intramuscular fat content were higher in the PB group. The lightness (p < 0.001), yellowness (p < 0.01), drip loss (p < 0.05) and protein content were lower in the PB group. Higher values of lightness (p < 0.05), yellowness (p < 0.05) and cholesterol content (p < 0.05) were observed in the boars. Oxidative stability of the meat (malondialdehyde content) was more favorable in the PB pigs. The effect of genotype and sex interaction was confirmed for the pH24 value (the highest value was found in the PB barrows and the lowest in the CH barrows, p < 0.001) and for the dry matter content (lowest in the PB gilts and highest in the CH barrows, p < 0.05). Overall, the PB pigs were characterized by inferior growth and carcass parameters, however, with more favorable parameters for meat quality.


Author(s):  
K. V. Gowrimanokari ◽  
R. Thiagarajan ◽  
R. Venkataramanan ◽  
H. Gopi

The present study was undertaken to study the effect of inbreeding on pre-weaning and sow performance traits of Large White Yorkshire pig population. Data on 7596 Large White Yorkshire pigs maintained at PGRIAS, Kattupakkam from the period of 1990 to 2015, were subjected to least square analysis to study the effect of inbreeding and other non-genetic factors viz., period, season, sex, parity, litter size at birth on various traits using general linear model. Inbreeding was calculated as Wright’s path co-efficient. The mean inbreeding co-efficient of LWY population studied was 2.45 %. Among 7596 animals in the pedigree, 2088 were inbred and the range of individual inbreeding co-efficient (F) was from 0 to 37.5 per cent. The average inbreeding in the inbred population was 8.92 %. The effect of inbreeding was found to be significant for weaning weight, pre-weaning average daily gain and litter weight at weaning, where the traits were found to increase with inbreeding (enhancement). The absence of depressive effect on fitness traits observed in the present study could be due to lower levels of inbreeding, which was possible due to periodic introduction of breeding animals and planned mating.


2004 ◽  
Vol 78 (1) ◽  
pp. 61-66 ◽  
Author(s):  
A. T. Kanengoni ◽  
K. Dzama ◽  
M. Chimonyo ◽  
J. Kusina ◽  
S. M. Maswaure

AbstractA study was conducted to compare the performance of Mukota, Large White (LW) and LW ✕ Mukota F1pigs given increasing levels of maize cob meal. Sixteen female weaners of each genotype were given, ad libitum, diets containing 0, 100, 200 and 300 g maize cob meal per kg of diet for 14 weeks. The diets were designed to contain similar levels of protein (ca. 160 g crude protein per kg) and energy (ca. 9 MJ metabolizable energy per kg). Average daily food intake per unit metabolic body weight (ADFI per kg M0·75), average daily gain (ADG) and food conversion ratio (FCR) were determined. The pigs were slaughtered and cold dressed mass (CDM) and backfat thickness were determined for each pig. The ADFI per kg M0·75was similar among the four diets for the three genotypes. The rate of decline in ADG was higher (P < 0·05) in the LW than in the other two genotypes. The FCR in the Mukota was poorer (P < 0·05) than that in the LW and the F1crosses. The Mukota had the highest (P < 0·05) backfat thickness (K5 and K7·5) values of the three genotypes across the four diets, followed by the F1crosses. The CDM values for the LW and the F1crosses were similar and were higher (P < 0·05) than those for the Mukota. The findings indicate that F1crosses and the Mukota were better able to utilize diets containing high levels of maize cob meal than LW pigs.


2003 ◽  
Vol 77 (1) ◽  
pp. 67-72 ◽  
Author(s):  
D. N. D’Souza ◽  
B. P. Mullan

AbstractSixty crossbred (Large White × Landrace × Duroc) pigs were used to compare the growth performance, carcass and pork quality characteristics of entire, surgically castrated and immunologically castrated male pigs from two Western Australian commercial genotypes (genotype A : ‘lean’ genotype and genotype B : propensity for increased subcutaneous fat deposition). Pigs from genotype B had a higher average daily gain and a superior food conversion ratio compared with pigs from genotype A. Entire males had lower backfat compared with surgically and immunologically castrated male pigs. Pork from entire males was drier, tougher and had lower overall acceptability scores compared with surgically or immunologically castrated male pigs. Pork from genotype A pigs had a lower m. longissimus thoracis (LT) ultimate pH and higher drip loss compared with pork from genotype B pigs. However, consumer taste panel assessment indicated that pork from genotype A pigs was more tender, had higher juiciness and overall acceptability scores compared with pork from genotype B pigs. Within genotype A, the consumer taste panel preferred pork from surgically castrated pigs compared with either entire or immunologically castrated pigs. Within genotype B, the consumer taste panel preferred pork from immunologically castrated pigs compared with either entire or surgically castrated male pigs. These results indicate that interactions between genotype and castration method can significantly influence eating quality of pork.


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