Isolation and Characterization of a New Streptomyces strain LG10 from an Unexploited Algerian Saharan Atlas

2021 ◽  
Vol 5 (1) ◽  
pp. 36-45
Author(s):  
Saïd Belghit ◽  
Omrane Toumatia ◽  
Mahfoud Bakli ◽  
Boubekeur Badji ◽  
Abdelghani Zitouni ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Biological Activities ◽  
Diaminopimelic Acid ◽  
Chemical Characteristics ◽  
16S Rdna Sequence ◽  
Streptomyces Strain ◽  
Isolation And Characterization ◽  
Aerial Hyphae

Abstract An actinobacterial strain named LG10 was isolated from a Saharan Atlas soil (Laghouat, Algeria). The aerial hyphae were yellowish-white on all culture media with rectiflexibiles spore chains, suggested that this bacterium attached to Streptomyces. Furthermore, LG10 contained chemical characteristics that were diagnostic for the genus Streptomyces, such as the presence of LL-diaminopimelic acid isomer (LL-DAP) and glycine amino acid. The hydrolysates of whole-cell included non-characteristic sugars. Comparative analysis of the 16S rDNA sequence displayed a similarity level of 100% with Streptomyces puniceus NRRL ISP-5058T. The antimicrobial activity of the LG10 strain was better in the culture medium MB5. Streptomyces strains are good sources of bioactive compounds with multiple biological activities.

Isolation and characterization of jadomycin L from Streptomyces venezuelae ISP5230 for solid tumor efficacy studies

2009 ◽  
Vol 81 (6) ◽  
pp. 1041-1049 ◽  
Author(s):  
David L. Jakeman ◽  
Stephanie N. Dupuis ◽  
Cathy L. Graham
Keyword(s):  
Natural Products ◽  
Solid Tumor ◽  
Culture Media ◽  
Biological Activities ◽  
Streptomyces Venezuelae ◽  
Gram Positive Bacteria ◽  
Isolation And Characterization ◽  
One Step ◽  
Efficacy Studies

Precursor-directed biosynthesis offers opportunities to modify natural products and obtain structurally complex metabolites without the need for chemical synthesis. However, such opportunities are limited owing to the inherent substrate specificity of biosynthetic enzymes. The jadomycins are a family of natural products produced by the soil microbe Streptomyces venezuelae ISP5230. Their biosynthesis contains one step that is potentially non-enzymatic, namely, the condensation of a biosynthetic aldehyde and an amino acid that leads to a uniquely substituted oxazolone ring. Variation of amino acids in the culture media enables the production of a wide array of substituted oxazolones. These analogs have been shown to have a variety of biological activities against cancer cell lines and also against Gram-positive bacteria. Herein, we report the first isolation and characterization of jadomycin L and jadomycin L aglycone from 8 L of bacterial culture for solid tumor efficacy studies.

Isolation and characterization of Actinopolyspora halophila, gen. et sp. nov., an extremely halophilic actinomycete

1975 ◽  
Vol 21 (10) ◽  
pp. 1500-1511 ◽  
Author(s):  
Margaret B. Gochnauer ◽  
Gary G. Leppard ◽  
Prayad Komaratat ◽  
Morris Kates ◽  
Thomas Novitsky ◽  
...  
Keyword(s):  
Cell Wall ◽  
Culture Medium ◽  
Halophilic Bacteria ◽  
Diaminopimelic Acid ◽  
Type Iv ◽  
Isolation And Characterization ◽  
The Family ◽  
Salt Requirement ◽  
Halophilic Actinomycete

An actinomycete, isolated as a contaminant of a culture medium containing 25% NaCl, has been classified as Actinopolyspora halophila gen. et sp. nov. in the family Nocardiaceae. The morphology and biochemical characteristics of this organism distinguish it from other members of the family Nocardiaceae and other genera possessing a type IV cell wall. It requires high NaCl concentrations for growth and can grow in saturated NaCl. The lowest concentration permitting growth in liquid medium is 12%, and on solid medium, 10%. Colonies developing at lower salt concentrations contain holes resembling viral plaques. No growth occurred in a medium containing 30% KCl instead of NaCl. This organism can grow in simple media with NH4+ salts as nitrogen source and different sugars and other compounds as carbon source. Though it has a salt requirement almost as great as the extremely halophilic rods and cocci, it differs from these in containing diaminopimelic acid and in sensitivity to lysozyme; both properties suggest that it has a mucopeptide cell wall. It also contains some phospholipids common to other actinomycetes, but does not contain any phytanyl ether linked lipids characteristic of other extremely halophilic bacteria.

Extraction, isolation and characterization of Bauhinia variegata flower

2020 ◽  
pp. 9-12
Author(s):  
Akanksha Gupta ◽  
Abhishek K Tripathi ◽  
Pushpraj S Gupta
Keyword(s):  
Biological Activities ◽  
Molecular Formula ◽  
Native Plant ◽  
Compound A ◽  
Active Constituents ◽  
Chromatographic Techniques ◽  
Isolation And Characterization ◽  
Soxhlet Apparatus ◽  
Bauhinia Variegata

Background: Bauhinia variegata Linn. is a native plant of Asia and China. B. variegata is found in tropical regions of the world. It belongs to family Leguminosae. It is used for diarrhea, hemorrhoids, constipation, piles, edema, leprosy, wounds, tumors, etc.  Objective: The objective of the present study was to perform extraction of B. variegata flower and isolation of active constituents from the extract. Materials and Methods: The ethanolic extraction of B. variegata flower was performed using the Soxhlet apparatus. The isolation of active constituents from the extract was performed using chromatographic techniques. In column chromatographic studies, n-hexane- [dichloromethane (DCM)] (2:8) was used as an eluting system and further purified through thin layer chromatography (TLC). Compound A and B were isolated through chromatographic techniques, then the molecular formula and characterization of these compounds were carried out with mass and infrared (IR) spectral analysis. Results and Discussion: The percentage yield of B. variegata ethanolic extract (BVE) was found to be 20.8% w/w. The different fractions were F1 having 12.5 grams with n-hexane, F2 (17.1 grams) with CH2Cl2, F3 (21.2 grams) with EtOAc, and F4 (13.4 grams) with EtOH. Compound A and B were isolated from the solvent fractions of n-hexane-DCM (2:8) and EtOAc-DCM (1:9), respectively. The compound A was characterized as 3-hydroxy-6-methoxy-2-phenyl-4H-chromen-4-one. The compound B was characterized as 3-hydroxy-6-methyl-2-phenyl-4H-chromen-4-one. Conclusion: Thus, B. variegata flowers possess active components that need to identify their biological activities.

scholarly journals Isolation and characterization of aristolactam alkaloids from the stem bark of Goniothalamus velutinus (Airy Shaw) and their biological activities

2018 ◽  
Vol 30 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Erum Iqbal ◽  
Linda B.L. Lim ◽  
Kamariah Abu Salim ◽  
Shaheen Faizi ◽  
Ayaz Ahmed ◽  
...  
Keyword(s):  
Biological Activities ◽  
Stem Bark ◽  
Isolation And Characterization

A review on phyto-pharmacology of Oxalis corniculata

2021 ◽  
Vol 24 ◽  
Author(s):  
Iqra Sarfraz ◽  
Azhar Rasul ◽  
Ghulam Hussain ◽  
Muhammad Ajmal Shah ◽  
Bushra Nageen ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Current Effort ◽  
Isolation And Characterization ◽  
Anti Cancer ◽  
Anti Oxidant ◽  
Anti Fungal ◽  
Warm Temperate

: Oxalis corniculata (Oxalidaceae) is a small decumbent and delicate appearing medicinal herb flourishing in warm temperate and tropical domains such as Pakistan and India. Main bioactive chemical constituents of Oxalis plant include several alkaloids, flavonoids, terpenoids, cardiac glycosides, saponins, phlobatannins along with steroids. Due to its polyphenolic, glycosides and flavonoid profile, it is proved to be protective in numerous ailments and exhibit various biological activities such as anti-fungal, anti-cancer, anti-oxidant, anti-bacterial, anti-diabetic, and cardioprotective. Moreover, bioactive phytochemicals from this plant possess significant wound healing potential. Our current effort intends to emphasize on the immense significance of this plant species, which have not been the subject matter of clinical trials and effective pharmacological studies, even though its favored usage has been stated. This review proposes that Oxalis corniculata possess potential for the cure of various diseases, however, further researches on isolation and characterization of bioactive compounds along with pre-clinical trials are compulsory to figure out its pharmacological applications.

Isolation and characterization of the ethynylestradiol-biodegrading microorganism Fusarium proliferatum strain HNS-1

2002 ◽  
Vol 45 (12) ◽  
pp. 175-179 ◽  
Author(s):  
J.H. Shi ◽  
Y. Suzuki ◽  
B.-D. Lee ◽  
S. Nakai ◽  
M. Hosomi
Keyword(s):  
Culture Medium ◽  
Polar Compounds ◽  
Sole Source ◽  
Order Rate Constant ◽  
Fusarium Proliferatum ◽  
Rrna Gene ◽  
28S Rrna ◽  
Isolation And Characterization ◽  
Phenolic Group

We cultivated hundreds of sediment, soil, and manure samples taken from rivers and farms in a medium containing ethynylestradiol (EE2) as the sole source of carbon, so that microorganisms in the samples would acclimatize to the presence of EE2. Finally, we isolated an EE2-degrading microorganism, designated as strain HNS-1, from a cowshed sample. Based on its partial nucleotide sequence (563 bp) of the 28S rRNA gene, strain HNS-1 was identified as Fusarium proliferatum. Over 15 days, F. proliferatum strain HNS-1 removed 97% of EE2 at an initial concentration of 25 mg.L−1, with a first-order rate constant of 0.6 d−1. Unknown products of EE2 degradation, which may be more polar compounds that have a phenolic group, remained in the culture medium.

scholarly journals The Quest for Phenolic Compounds from Macroalgae: A Review of Extraction and Identification Methodologies

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 847 ◽  
Author(s):  
Sónia A. O. Santos ◽  
Rafael Félix ◽  
Adriana C. S. Pais ◽  
Sílvia M. Rocha ◽  
Armando J. D. Silvestre
Keyword(s):  
Phenolic Compounds ◽  
Biological Activities ◽  
Mass Spectrometry Data ◽  
Brown Macroalgae ◽  
Isolation And Characterization ◽  
Analytical Strategies ◽  
Critical Revision ◽  
Identification Techniques ◽  
First Time

The current interest of the scientific community for the exploitation of high-value compounds from macroalgae is related to the increasing knowledge of their biological activities and health benefits. Macroalgae phenolic compounds, particularly phlorotannins, have gained particular attention due to their specific bioactivities, including antioxidant, antiproliferative, or antidiabetic. Notwithstanding, the characterization of macroalgae phenolic compounds is a multi-step task, with high challenges associated with their isolation and characterization, due to the highly complex and polysaccharide-rich matrix of macroalgae. Therefore, this fraction is far from being fully explored. In fact, a critical revision of the extraction and characterization methodologies already used in the analysis of phenolic compounds from macroalgae is lacking in the literature, and it is of uttermost importance to compile validated methodologies and discourage misleading practices. The aim of this review is to discuss the state-of-the-art of phenolic compounds already identified in green, red, and brown macroalgae, reviewing their structural classification, as well as critically discussing extraction methodologies, chromatographic separation techniques, and the analytical strategies for their characterization, including information about structural identification techniques and key spectroscopic profiles. For the first time, mass spectrometry data of phlorotannins, a chemical family quite exclusive of macroalgae, is compiled and discussed.

The isolation and characterization of subcellular fractions from pigmented and unpigmented cells of Rhodopseudomonas spheroides

1968 ◽  
Vol 170 (1020) ◽  
pp. 229-246 ◽  
Keyword(s):  
Nucleic Acid ◽  
Cytoplasmic Membrane ◽  
Sucrose Density Gradient ◽  
Subcellular Fractions ◽  
Diaminopimelic Acid ◽  
Pure Oxygen ◽  
Simple Method ◽  
Isolation And Characterization ◽  
Rhodopseudomonas Spheroides

Current ideas on the nature and development of the chromatophores of photosynthetic bacteria are reviewed. A simple method of obtaining purified chromatophores by sucrose density gradient centrifuging of cell-free extracts of Rhodopseudomonas spheroides is described. Such prepara­tions consist of about 60% protein, 20% phospholipid and 10% pigment, most of which is bacteriochlorophyll. Small quantities of carbohydrate, but only traces of nucleic acid, are found. The material was fairly homogeneous on electron microscopy. Rps. spheroides was also grown under pure oxygen in the dark. A particulate preparation from cells cultured under these conditions was similar to the chromatophores with respect to its high content of protein and of phospholipid but had a much greater content of nucleic acid and no bacteriochlorophyll. In addition, it contained amino sugars and diaminopimelic acid which are not found in chromatophores. These differences in chemical composition were correlated with the electron microscope appearances of the different subcellular fractions. It is concluded that the particulate preparation from cells grown under oxygen represents the cytoplasmic membrane of the micro-organism, but as isolated it is heavily contaminated with fragments of the cell wall from which it cannot be readily separated.

Isolation and Characterization of a Phosphate-Solubilizing Bacterium Pseudochrobactrum sp. PSB13 from Petroleum-Contaminated Drill Cuttings

2014 ◽  
Vol 955-959 ◽  
pp. 407-410
Author(s):  
Li Bin Zhao ◽  
Xin Xin Wang ◽  
Chen Li ◽  
Yu Chen ◽  
Wei An ◽  
...  
Keyword(s):  
Drilling Process ◽  
Phosphate Solubilizing Bacteria ◽  
Phenotypic Characteristics ◽  
Drill Cuttings ◽  
16S Rdna Sequence ◽  
Isolation And Characterization ◽  
Bacterium Strain ◽  
Phosphate Solubilizing ◽  
First Time

Phosphate-solubilizing bacteria were extensively studied in many environment. However, little is known about them in drill cuttings, as wastes from drilling process. A phosphate-solubilizing bacterium strain PSB13 was isolated from petroleum-contaminated drill cuttings. This strain was identified asPseudochrobactrumsp. based on its 16S rDNA sequence and phenotypic characteristics. This strain could solubilize 97.6 μg/ml phosphates in 6 days when grown in NBRIP liquid medium. The increase in solubilization of phosphate coincided with the drop in pH, which indicates organic acid was responsible for the phosphate-solubilization. Phosphate-solubilizing bacterium was reported in drill cuttings for the first time, which suggests its potential in the bioremediation of petroleum-contaminated drill cuttings.

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